Chitinophagaceae bacterium IBVUCB1
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3055 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z5SFG6|A0A1Z5SFG6_9BACT Uncharacterized protein OS=Chitinophagaceae bacterium IBVUCB1 OX=1985173 GN=CAP35_12890 PE=4 SV=1
MM1 pKa = 8.23 VIEE4 pKa = 4.25 TEE6 pKa = 4.06 IKK8 pKa = 10.3 DD9 pKa = 3.59 VLFNYY14 pKa = 9.15 PVTFEE19 pKa = 5.2 DD20 pKa = 3.35 VDD22 pKa = 3.96 NEE24 pKa = 4.08 ILSEE28 pKa = 4.01 TFYY31 pKa = 10.9 IVTWNHH37 pKa = 5.49 SEE39 pKa = 4.04 PFIMAWDD46 pKa = 3.67 FDD48 pKa = 3.57 EE49 pKa = 4.4 WQLVIQSKK57 pKa = 9.74 APHH60 pKa = 5.5 MAKK63 pKa = 10.32 LFEE66 pKa = 4.05 YY67 pKa = 10.99 ALFKK71 pKa = 11.36 VIDD74 pKa = 4.43 DD75 pKa = 4.31 YY76 pKa = 12.36 NNGFCNFQQ84 pKa = 3.14
Molecular weight: 10.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.825
IPC2_protein 4.075
IPC_protein 3.973
Toseland 3.795
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.859
Rodwell 3.808
Grimsley 3.706
Solomon 3.935
Lehninger 3.884
Nozaki 4.075
DTASelect 4.228
Thurlkill 3.834
EMBOSS 3.872
Sillero 4.088
Patrickios 1.914
IPC_peptide 3.935
IPC2_peptide 4.075
IPC2.peptide.svr19 3.992
Protein with the highest isoelectric point:
>tr|A0A1Z5SCG0|A0A1Z5SCG0_9BACT Uncharacterized protein OS=Chitinophagaceae bacterium IBVUCB1 OX=1985173 GN=CAP35_07225 PE=4 SV=1
MM1 pKa = 7.74 RR2 pKa = 11.84 KK3 pKa = 8.93 GVSMRR8 pKa = 11.84 GIGYY12 pKa = 9.48 LLTISVSSVVRR23 pKa = 11.84 YY24 pKa = 9.38 IRR26 pKa = 11.84 RR27 pKa = 11.84 ISAVIAKK34 pKa = 7.33 PTHH37 pKa = 6.35 AEE39 pKa = 3.91 CQQVYY44 pKa = 9.63 EE45 pKa = 4.1 VDD47 pKa = 3.38 EE48 pKa = 4.21 MQTYY52 pKa = 10.12 IGSKK56 pKa = 8.9 QEE58 pKa = 3.75 VNRR61 pKa = 11.84 VYY63 pKa = 10.92 IMYY66 pKa = 9.86 CINRR70 pKa = 11.84 ATRR73 pKa = 11.84 EE74 pKa = 4.12 VISFIVGRR82 pKa = 11.84 RR83 pKa = 11.84 TKK85 pKa = 10.83 EE86 pKa = 3.82 NLNKK90 pKa = 10.21 LVNAVLEE97 pKa = 4.14 LSPKK101 pKa = 10.23 RR102 pKa = 11.84 ICTDD106 pKa = 2.63 GLNIYY111 pKa = 9.97 RR112 pKa = 11.84 SIISPPLHH120 pKa = 6.38 SVSKK124 pKa = 10.6 YY125 pKa = 6.82 GTNRR129 pKa = 11.84 IEE131 pKa = 4.37 RR132 pKa = 11.84 KK133 pKa = 9.62 NLSLRR138 pKa = 11.84 TDD140 pKa = 4.0 LKK142 pKa = 10.25 RR143 pKa = 11.84 LSRR146 pKa = 11.84 RR147 pKa = 11.84 TICFSRR153 pKa = 11.84 SLPMLVACLRR163 pKa = 11.84 IYY165 pKa = 10.69 FWGG168 pKa = 3.71
Molecular weight: 19.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 9.75
IPC_protein 10.452
Toseland 10.35
ProMoST 10.116
Dawson 10.54
Bjellqvist 10.292
Wikipedia 10.76
Rodwell 10.76
Grimsley 10.628
Solomon 10.599
Lehninger 10.555
Nozaki 10.379
DTASelect 10.277
Thurlkill 10.409
EMBOSS 10.76
Sillero 10.467
Patrickios 10.394
IPC_peptide 10.599
IPC2_peptide 9.428
IPC2.peptide.svr19 8.451
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3055
0
3055
1028320
38
3714
336.6
37.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.967 ± 0.044
1.044 ± 0.016
5.29 ± 0.031
5.275 ± 0.056
4.372 ± 0.031
6.799 ± 0.05
1.982 ± 0.024
7.199 ± 0.037
6.74 ± 0.052
8.889 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.733 ± 0.026
5.643 ± 0.044
3.783 ± 0.027
3.83 ± 0.027
4.013 ± 0.031
5.92 ± 0.043
6.324 ± 0.083
6.489 ± 0.03
1.202 ± 0.017
4.506 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here