Dentipellis fragilis
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11715 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y9Z0W0|A0A4Y9Z0W0_9AGAM Acetate--CoA ligase OS=Dentipellis fragilis OX=205917 GN=EVG20_g3933 PE=3 SV=1
MM1 pKa = 7.34 FATLFTVTLLSAGAIKK17 pKa = 10.46 GALAGLAINSPTLVQCEE34 pKa = 4.09 DD35 pKa = 3.6 AHH37 pKa = 6.88 VSWASTKK44 pKa = 10.43 GPYY47 pKa = 10.08 NLIVTPGDD55 pKa = 3.95 EE56 pKa = 4.39 PCGDD60 pKa = 4.68 AIVDD64 pKa = 4.35 LGDD67 pKa = 3.93 HH68 pKa = 7.39 DD69 pKa = 4.75 GTTMTYY75 pKa = 10.61 SVALPAGQKK84 pKa = 10.12 VLLSLQDD91 pKa = 3.73 ANGDD95 pKa = 3.93 EE96 pKa = 4.53 AWSQEE101 pKa = 3.91 LTVQAGSNTSCLPALLQVSSSISTSSVVPTVSSAKK136 pKa = 9.53 FALSSTVVPLTAVTPATTLSAFALPSSDD164 pKa = 3.42 PAAAAPAPSDD174 pKa = 3.54 TVAGDD179 pKa = 4.38 DD180 pKa = 4.41 SDD182 pKa = 4.59 SDD184 pKa = 4.01 SPSVVGGAANAASNPFSGALAMHH207 pKa = 6.27 QLYY210 pKa = 8.27 TPAMLLSALATAFFVALL227 pKa = 4.01
Molecular weight: 22.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.554
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.783
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.745
Nozaki 3.923
DTASelect 4.215
Thurlkill 3.643
EMBOSS 3.783
Sillero 3.91
Patrickios 0.896
IPC_peptide 3.783
IPC2_peptide 3.884
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|A0A4Y9XNJ3|A0A4Y9XNJ3_9AGAM Uncharacterized protein OS=Dentipellis fragilis OX=205917 GN=EVG20_g10886 PE=4 SV=1
MM1 pKa = 7.84 PLQAPMRR8 pKa = 11.84 PSDD11 pKa = 3.75 AVSPAAAHH19 pKa = 6.79 RR20 pKa = 11.84 KK21 pKa = 6.57 LTPAARR27 pKa = 11.84 PNSFHH32 pKa = 6.87 NKK34 pKa = 6.68 QHH36 pKa = 5.06 TTNIPRR42 pKa = 11.84 TQTRR46 pKa = 11.84 SRR48 pKa = 11.84 RR49 pKa = 11.84 WQQNWNGTTTKK60 pKa = 10.31 NDD62 pKa = 3.27 RR63 pKa = 11.84 AAGCTGLDD71 pKa = 3.32 ARR73 pKa = 11.84 HH74 pKa = 5.77 VLWSLLSTNVMAGAVDD90 pKa = 3.44 PHH92 pKa = 6.53 TYY94 pKa = 9.5 ICAGQPCGACVKK106 pKa = 10.26 DD107 pKa = 3.4 LRR109 pKa = 11.84 GRR111 pKa = 11.84 NVGEE115 pKa = 3.89 KK116 pKa = 10.29 RR117 pKa = 11.84 EE118 pKa = 4.28 ACVGRR123 pKa = 11.84 AGPSEE128 pKa = 4.1 ISQVIPIPKK137 pKa = 9.27 ISIMQGARR145 pKa = 11.84 ARR147 pKa = 11.84 AFAFPDD153 pKa = 3.05 KK154 pKa = 10.08 KK155 pKa = 10.97 ARR157 pKa = 11.84 IISYY161 pKa = 6.83 TSRR164 pKa = 11.84 RR165 pKa = 11.84 HH166 pKa = 5.04 SAPPHH171 pKa = 6.11 DD172 pKa = 4.16 TLISNPDD179 pKa = 3.55 TGTMTEE185 pKa = 4.54 DD186 pKa = 2.98 EE187 pKa = 4.72 RR188 pKa = 11.84 EE189 pKa = 4.19 RR190 pKa = 11.84 PTATAEE196 pKa = 3.97 NDD198 pKa = 3.0 NDD200 pKa = 3.53 IANVWRR206 pKa = 11.84 APTPYY211 pKa = 10.47 ASSRR215 pKa = 11.84 SDD217 pKa = 3.09 AFAPDD222 pKa = 3.48 TQEE225 pKa = 3.46 QQQRR229 pKa = 11.84 HH230 pKa = 4.64 QHH232 pKa = 5.3 HH233 pKa = 6.62 CHH235 pKa = 5.72 YY236 pKa = 10.49 APHH239 pKa = 7.2 ALRR242 pKa = 11.84 GANSLATHH250 pKa = 7.44 LYY252 pKa = 6.11 PTSGTPRR259 pKa = 11.84 APPPQAQTPRR269 pKa = 11.84 AARR272 pKa = 11.84 GAAPRR277 pKa = 11.84 PPPRR281 pKa = 11.84 GPSRR285 pKa = 11.84 PPRR288 pKa = 11.84 TSGARR293 pKa = 11.84 TRR295 pKa = 11.84 QTGASQLGMVTVTVTGVEE313 pKa = 4.2 CWSRR317 pKa = 11.84 SCNVNGDD324 pKa = 3.53 RR325 pKa = 11.84 EE326 pKa = 4.07 RR327 pKa = 11.84 DD328 pKa = 3.22 VDD330 pKa = 4.2 SFSLVSFRR338 pKa = 11.84 QLAAPAEE345 pKa = 4.24 MGMEE349 pKa = 3.62 IAEE352 pKa = 4.15 FAFTFKK358 pKa = 9.97 FTSRR362 pKa = 11.84 GLGDD366 pKa = 4.15 PGAKK370 pKa = 9.22 RR371 pKa = 11.84 CIRR374 pKa = 11.84 SRR376 pKa = 11.84 SRR378 pKa = 11.84 TEE380 pKa = 3.53 TMRR383 pKa = 11.84 ISILRR388 pKa = 11.84 SDD390 pKa = 3.98 LASYY394 pKa = 10.12 CRR396 pKa = 11.84 NNANMQTSTTYY407 pKa = 10.7 HH408 pKa = 5.4 EE409 pKa = 5.32 AKK411 pKa = 10.34 RR412 pKa = 11.84 SMCLCLCPATQPGTTQTTYY431 pKa = 11.67 ALRR434 pKa = 11.84 TRR436 pKa = 11.84 RR437 pKa = 11.84 RR438 pKa = 11.84 GIRR441 pKa = 11.84 SDD443 pKa = 3.03 KK444 pKa = 10.76 HH445 pKa = 4.83 KK446 pKa = 11.03 APAKK450 pKa = 9.77 RR451 pKa = 11.84 APEE454 pKa = 3.71 PHH456 pKa = 7.08 LLRR459 pKa = 11.84 EE460 pKa = 3.97 QADD463 pKa = 3.41 IEE465 pKa = 4.46 VADD468 pKa = 4.06 RR469 pKa = 11.84 RR470 pKa = 11.84 RR471 pKa = 11.84 GRR473 pKa = 11.84 EE474 pKa = 3.36 HH475 pKa = 7.02 RR476 pKa = 11.84 LAVEE480 pKa = 3.95 ARR482 pKa = 11.84 RR483 pKa = 11.84 LRR485 pKa = 11.84 EE486 pKa = 3.97 EE487 pKa = 4.04 QEE489 pKa = 4.36 HH490 pKa = 5.64 VSARR494 pKa = 11.84 HH495 pKa = 6.55 DD496 pKa = 3.99 GSQHH500 pKa = 5.79 SISRR504 pKa = 11.84 VASRR508 pKa = 11.84 EE509 pKa = 3.84 LGHH512 pKa = 6.25 WVSHH516 pKa = 6.55 IGRR519 pKa = 11.84 TRR521 pKa = 11.84 RR522 pKa = 11.84 RR523 pKa = 11.84 KK524 pKa = 9.94 RR525 pKa = 11.84 EE526 pKa = 3.54 EE527 pKa = 3.57 AKK529 pKa = 10.38 IGEE532 pKa = 4.26 KK533 pKa = 9.24 TVYY536 pKa = 10.52 SKK538 pKa = 7.4 THH540 pKa = 6.23 RR541 pKa = 11.84 CGSDD545 pKa = 2.97 PAXGVGCKK553 pKa = 10.37 NSDD556 pKa = 3.55 SAA558 pKa = 5.01
Molecular weight: 61.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.357
IPC2_protein 9.472
IPC_protein 10.321
Toseland 10.599
ProMoST 10.35
Dawson 10.701
Bjellqvist 10.452
Wikipedia 10.921
Rodwell 10.789
Grimsley 10.745
Solomon 10.833
Lehninger 10.804
Nozaki 10.628
DTASelect 10.423
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.643
Patrickios 10.467
IPC_peptide 10.847
IPC2_peptide 9.765
IPC2.peptide.svr19 8.601
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11715
0
11715
5974488
38
4685
510.0
56.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.399 ± 0.022
1.365 ± 0.009
5.552 ± 0.014
5.635 ± 0.02
3.552 ± 0.014
6.408 ± 0.017
2.773 ± 0.01
4.507 ± 0.017
4.099 ± 0.017
9.207 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.042 ± 0.008
3.063 ± 0.012
7.01 ± 0.027
3.628 ± 0.014
6.771 ± 0.02
8.674 ± 0.026
5.965 ± 0.013
6.312 ± 0.014
1.397 ± 0.009
2.533 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here