Citrus leaf rugose virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Ilarvirus

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q66108|Q66108_9BROM ATP-dependent helicase OS=Citrus leaf rugose virus OX=37126 PE=3 SV=1
MM1 pKa = 7.75DD2 pKa = 4.28TLLHH6 pKa = 5.93FVKK9 pKa = 10.75FSKK12 pKa = 10.67VLSFLDD18 pKa = 3.62VNATFEE24 pKa = 4.42IPLSDD29 pKa = 3.67EE30 pKa = 3.97QIKK33 pKa = 10.44YY34 pKa = 8.44IQLWMLRR41 pKa = 11.84DD42 pKa = 3.77VVIPAMKK49 pKa = 10.34FSWCGFILLDD59 pKa = 3.72KK60 pKa = 11.44LEE62 pKa = 4.47MEE64 pKa = 5.79LEE66 pKa = 4.38RR67 pKa = 11.84IYY69 pKa = 11.07QNDD72 pKa = 3.83YY73 pKa = 10.7SPPILMHH80 pKa = 6.29VPSQLVTDD88 pKa = 4.58EE89 pKa = 4.5LADD92 pKa = 4.9FIDD95 pKa = 4.14EE96 pKa = 4.32FGEE99 pKa = 4.47VPEE102 pKa = 4.18EE103 pKa = 4.43AYY105 pKa = 10.72LSPEE109 pKa = 3.85EE110 pKa = 4.12RR111 pKa = 11.84EE112 pKa = 4.55KK113 pKa = 10.58IHH115 pKa = 6.98EE116 pKa = 4.1PTFLNHH122 pKa = 5.48EE123 pKa = 4.26HH124 pKa = 6.79WGSEE128 pKa = 3.96SSGSSFLDD136 pKa = 4.35EE137 pKa = 5.26IEE139 pKa = 4.04MLYY142 pKa = 11.05DD143 pKa = 3.39EE144 pKa = 4.66TVKK147 pKa = 11.13DD148 pKa = 4.43DD149 pKa = 3.57VVQDD153 pKa = 3.72VSADD157 pKa = 3.56MPKK160 pKa = 10.39DD161 pKa = 3.75YY162 pKa = 11.15QIMWNDD168 pKa = 3.57GAVDD172 pKa = 3.98AVWSSKK178 pKa = 10.98FEE180 pKa = 4.04VEE182 pKa = 4.53EE183 pKa = 4.07PPKK186 pKa = 10.65PKK188 pKa = 10.38FKK190 pKa = 10.29PQQTDD195 pKa = 4.19RR196 pKa = 11.84ICDD199 pKa = 3.41PVIIQDD205 pKa = 4.7AINDD209 pKa = 3.63IFPFHH214 pKa = 7.83HH215 pKa = 6.62EE216 pKa = 3.71MDD218 pKa = 3.45DD219 pKa = 3.86TYY221 pKa = 11.15FQTWVEE227 pKa = 4.15TQDD230 pKa = 3.07ISLEE234 pKa = 4.18VSKK237 pKa = 10.96CWLDD241 pKa = 3.26ASNFKK246 pKa = 10.96DD247 pKa = 3.78FTKK250 pKa = 10.69GQQTYY255 pKa = 10.28AVPAYY260 pKa = 10.45QSGATSKK267 pKa = 10.53RR268 pKa = 11.84VNTQRR273 pKa = 11.84EE274 pKa = 3.87TLLAVKK280 pKa = 9.35KK281 pKa = 10.43RR282 pKa = 11.84NMNIPEE288 pKa = 4.22LQSTFDD294 pKa = 4.09LDD296 pKa = 4.86AEE298 pKa = 4.38VATCFKK304 pKa = 10.54RR305 pKa = 11.84FCTHH309 pKa = 5.51VVDD312 pKa = 4.41IPRR315 pKa = 11.84SKK317 pKa = 10.69RR318 pKa = 11.84LPQMLGTEE326 pKa = 3.92IQFFSDD332 pKa = 3.37YY333 pKa = 10.92LAGKK337 pKa = 9.45NPPLSEE343 pKa = 4.07YY344 pKa = 10.43QGPLTLQSLDD354 pKa = 3.65KK355 pKa = 10.5YY356 pKa = 8.34MHH358 pKa = 5.5MVKK361 pKa = 9.98TIVKK365 pKa = 8.75PVEE368 pKa = 4.24DD369 pKa = 3.84NSLKK373 pKa = 10.63FEE375 pKa = 4.58RR376 pKa = 11.84PLCATITYY384 pKa = 8.26HH385 pKa = 6.97KK386 pKa = 9.85KK387 pKa = 10.32GIVMQSSPLFLSAMSRR403 pKa = 11.84LFYY406 pKa = 10.61VLKK409 pKa = 10.64SKK411 pKa = 10.19IHH413 pKa = 5.86IPSGKK418 pKa = 7.2WHH420 pKa = 6.3QLFTLDD426 pKa = 4.7AAAFDD431 pKa = 3.55AAKK434 pKa = 9.66WFKK437 pKa = 10.72EE438 pKa = 3.7VDD440 pKa = 3.71FSKK443 pKa = 10.64FDD445 pKa = 3.3KK446 pKa = 11.19SQGEE450 pKa = 4.19LHH452 pKa = 6.89HH453 pKa = 6.79KK454 pKa = 6.68VQKK457 pKa = 10.35MIFDD461 pKa = 4.56LLKK464 pKa = 10.61LPPEE468 pKa = 4.1FVEE471 pKa = 4.27MWFTSHH477 pKa = 5.59EE478 pKa = 3.92RR479 pKa = 11.84SYY481 pKa = 10.12ITDD484 pKa = 3.39RR485 pKa = 11.84DD486 pKa = 3.85TGVGFAVDD494 pKa = 4.05FQRR497 pKa = 11.84RR498 pKa = 11.84TGDD501 pKa = 3.07ANTYY505 pKa = 10.16LGNTLVTLICLARR518 pKa = 11.84VYY520 pKa = 10.32DD521 pKa = 3.84LCDD524 pKa = 3.54PKK526 pKa = 10.22ITFVIASGDD535 pKa = 3.51DD536 pKa = 3.52SLIGSVEE543 pKa = 4.01EE544 pKa = 4.07LPRR547 pKa = 11.84APEE550 pKa = 3.79HH551 pKa = 6.87LFTSLFNFEE560 pKa = 5.0AKK562 pKa = 10.15FPHH565 pKa = 5.93NQPFICSKK573 pKa = 10.38ILVSVDD579 pKa = 3.52LVCGGRR585 pKa = 11.84EE586 pKa = 4.21VIAVPNPAKK595 pKa = 10.65LLIRR599 pKa = 11.84MGRR602 pKa = 11.84RR603 pKa = 11.84DD604 pKa = 3.97CQYY607 pKa = 10.86QALDD611 pKa = 3.78DD612 pKa = 5.92LYY614 pKa = 10.84TSWLDD619 pKa = 3.25VIYY622 pKa = 10.19YY623 pKa = 10.13FRR625 pKa = 11.84DD626 pKa = 3.06SRR628 pKa = 11.84VCEE631 pKa = 4.08KK632 pKa = 10.94VADD635 pKa = 4.0LCAYY639 pKa = 8.84RR640 pKa = 11.84QTRR643 pKa = 11.84RR644 pKa = 11.84SSMYY648 pKa = 10.48LLSALLSLPSCFANKK663 pKa = 10.08KK664 pKa = 9.67KK665 pKa = 10.73FKK667 pKa = 10.18LLCYY671 pKa = 9.95HH672 pKa = 6.46LTEE675 pKa = 4.43EE676 pKa = 4.15EE677 pKa = 4.58CLRR680 pKa = 11.84KK681 pKa = 9.9AKK683 pKa = 10.1PSSITNGHH691 pKa = 5.06VEE693 pKa = 3.77KK694 pKa = 10.15EE695 pKa = 3.9QRR697 pKa = 11.84VNGCRR702 pKa = 11.84DD703 pKa = 2.91SRR705 pKa = 11.84IIQARR710 pKa = 11.84SEE712 pKa = 4.11IPAEE716 pKa = 3.9EE717 pKa = 4.11SRR719 pKa = 11.84RR720 pKa = 11.84IQKK723 pKa = 10.0NSPRR727 pKa = 11.84PDD729 pKa = 3.2KK730 pKa = 11.18GFQAYY735 pKa = 8.14PRR737 pKa = 11.84RR738 pKa = 11.84KK739 pKa = 9.91SRR741 pKa = 11.84GCWMAYY747 pKa = 8.26VPKK750 pKa = 10.18ILQAHH755 pKa = 6.85DD756 pKa = 3.41SSGRR760 pKa = 11.84IPDD763 pKa = 3.29AVTRR767 pKa = 11.84KK768 pKa = 9.53SFSSIRR774 pKa = 11.84GSLGLEE780 pKa = 4.07PDD782 pKa = 4.09PSCGIGVGARR792 pKa = 11.84TTSSFSGKK800 pKa = 6.96NTRR803 pKa = 11.84PRR805 pKa = 11.84HH806 pKa = 5.02RR807 pKa = 11.84FRR809 pKa = 11.84VEE811 pKa = 3.75GVHH814 pKa = 5.51QSSRR818 pKa = 11.84GYY820 pKa = 9.7PADD823 pKa = 3.44LPAYY827 pKa = 9.56NRR829 pKa = 11.84RR830 pKa = 11.84SSS832 pKa = 3.07

Molecular weight: 95.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q66107|Q66107_9BROM RNA-directed RNA polymerase 2a OS=Citrus leaf rugose virus OX=37126 PE=4 SV=1
MM1 pKa = 7.85ANRR4 pKa = 11.84SNAIEE9 pKa = 4.22VNGVWYY15 pKa = 10.55NRR17 pKa = 11.84ADD19 pKa = 3.66NAPVANARR27 pKa = 11.84GRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84PTNRR35 pKa = 11.84SRR37 pKa = 11.84NWAQGQRR44 pKa = 11.84SQPQRR49 pKa = 11.84MVVGSMPIDD58 pKa = 4.19TITWKK63 pKa = 10.63SFPGEE68 pKa = 3.8QWHH71 pKa = 6.15EE72 pKa = 3.73FSGFSFPDD80 pKa = 2.71AWGSAKK86 pKa = 9.61IAYY89 pKa = 10.09ASMRR93 pKa = 11.84TEE95 pKa = 3.75LGKK98 pKa = 10.39IKK100 pKa = 10.35SLHH103 pKa = 6.01HH104 pKa = 4.54TTKK107 pKa = 10.27VYY109 pKa = 10.84SVMYY113 pKa = 10.59GFTCKK118 pKa = 10.28ADD120 pKa = 3.74GYY122 pKa = 11.26AGFMDD127 pKa = 4.58NFDD130 pKa = 4.09SANATGPVAPNRR142 pKa = 11.84IRR144 pKa = 11.84VKK146 pKa = 9.99AAKK149 pKa = 9.54YY150 pKa = 9.35CARR153 pKa = 11.84QLVLPPGSTVADD165 pKa = 3.6LRR167 pKa = 11.84DD168 pKa = 3.39NYY170 pKa = 10.82NFVWEE175 pKa = 4.74FDD177 pKa = 3.53AAPAAGTANIISVTKK192 pKa = 9.59FYY194 pKa = 11.26VSTVPLPGIKK204 pKa = 9.85PPPNFLVCEE213 pKa = 4.23MDD215 pKa = 4.76DD216 pKa = 3.58KK217 pKa = 11.87

Molecular weight: 24.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2384

217

1051

596.0

67.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.299 ± 0.834

1.971 ± 0.179

7.005 ± 0.346

5.201 ± 0.507

4.404 ± 0.623

4.488 ± 0.417

2.391 ± 0.234

5.705 ± 0.296

6.04 ± 0.33

8.263 ± 0.754

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.894 ± 0.506

4.027 ± 0.635

5.076 ± 0.527

2.894 ± 0.564

6.334 ± 0.31

8.347 ± 0.203

5.663 ± 0.37

7.341 ± 0.464

1.552 ± 0.21

3.104 ± 0.321
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski