Pseudomonas phage NV1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Vicosavirus; Pseudomonas virus NV1

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L0HPM7|A0A2L0HPM7_9CAUD Uncharacterized protein OS=Pseudomonas phage NV1 OX=2079543 GN=NV1_p30 PE=4 SV=1
MM1 pKa = 7.93NMEE4 pKa = 4.42EE5 pKa = 5.23LILLIDD11 pKa = 3.53EE12 pKa = 4.25TRR14 pKa = 11.84ANEE17 pKa = 4.13GATTAKK23 pKa = 10.46VLEE26 pKa = 4.13FLQNKK31 pKa = 9.2DD32 pKa = 3.23NPLKK36 pKa = 10.72EE37 pKa = 4.05RR38 pKa = 11.84GIVLANAGAYY48 pKa = 9.63ASSEE52 pKa = 4.28VYY54 pKa = 10.21YY55 pKa = 10.34CGPDD59 pKa = 3.21GHH61 pKa = 6.82RR62 pKa = 11.84DD63 pKa = 3.49KK64 pKa = 11.63VSLYY68 pKa = 11.0DD69 pKa = 3.28DD70 pKa = 5.37FYY72 pKa = 10.71WEE74 pKa = 3.9RR75 pKa = 11.84HH76 pKa = 3.78EE77 pKa = 4.09TRR79 pKa = 11.84TFGEE83 pKa = 3.96ILEE86 pKa = 4.58TTIEE90 pKa = 4.42HH91 pKa = 5.96YY92 pKa = 11.1LEE94 pKa = 4.02LHH96 pKa = 6.76EE97 pKa = 6.19DD98 pKa = 3.57LDD100 pKa = 3.71VDD102 pKa = 3.68YY103 pKa = 11.15DD104 pKa = 4.34LVVEE108 pKa = 5.47TDD110 pKa = 4.38GFWNWVATSEE120 pKa = 4.19DD121 pKa = 3.38AKK123 pKa = 11.41ALVILDD129 pKa = 3.79FVEE132 pKa = 4.14NALAEE137 pKa = 4.32ATHH140 pKa = 6.9DD141 pKa = 3.41WW142 pKa = 3.63

Molecular weight: 16.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L0HPR5|A0A2L0HPR5_9CAUD Putative terminase small subunit OS=Pseudomonas phage NV1 OX=2079543 GN=NV1_p64 PE=1 SV=1
MM1 pKa = 7.73LSPKK5 pKa = 10.17QLMIGKK11 pKa = 8.15EE12 pKa = 3.83KK13 pKa = 11.09NMASRR18 pKa = 11.84ALTAGEE24 pKa = 4.1WMDD27 pKa = 3.65LGYY30 pKa = 10.26IIRR33 pKa = 11.84TGEE36 pKa = 3.77RR37 pKa = 11.84SHH39 pKa = 6.69CRR41 pKa = 11.84NARR44 pKa = 11.84GRR46 pKa = 11.84ALFFRR51 pKa = 11.84DD52 pKa = 3.46QVRR55 pKa = 11.84VRR57 pKa = 11.84HH58 pKa = 5.3YY59 pKa = 10.75AVAPRR64 pKa = 11.84GQWVFVPYY72 pKa = 10.68NN73 pKa = 3.21

Molecular weight: 8.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

13737

60

1057

214.6

23.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.896 ± 0.542

1.005 ± 0.14

6.086 ± 0.255

6.442 ± 0.329

3.778 ± 0.18

8.481 ± 0.331

2.002 ± 0.221

4.841 ± 0.19

5.933 ± 0.256

8.051 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.693 ± 0.138

4.666 ± 0.266

4.484 ± 0.236

4.419 ± 0.277

5.387 ± 0.256

5.38 ± 0.268

5.583 ± 0.269

7.112 ± 0.206

1.58 ± 0.15

3.181 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski