Amino acid dipepetide frequency for Pseudomonas phage NV1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.901AlaAla: 9.901 ± 1.873
0.801AlaCys: 0.801 ± 0.275
4.514AlaAsp: 4.514 ± 0.79
6.261AlaGlu: 6.261 ± 0.764
2.912AlaPhe: 2.912 ± 0.509
9.319AlaGly: 9.319 ± 1.128
1.456AlaHis: 1.456 ± 0.361
4.15AlaIle: 4.15 ± 0.552
5.824AlaLys: 5.824 ± 0.812
6.407AlaLeu: 6.407 ± 0.797
2.475AlaMet: 2.475 ± 0.379
3.494AlaAsn: 3.494 ± 0.589
4.077AlaPro: 4.077 ± 0.748
4.077AlaGln: 4.077 ± 0.698
3.422AlaArg: 3.422 ± 0.481
4.15AlaSer: 4.15 ± 0.438
4.878AlaThr: 4.878 ± 0.637
6.698AlaVal: 6.698 ± 0.84
1.165AlaTrp: 1.165 ± 0.328
2.985AlaTyr: 2.985 ± 0.48
0.0AlaXaa: 0.0 ± 0.0
Cys
0.874CysAla: 0.874 ± 0.231
0.218CysCys: 0.218 ± 0.138
0.728CysAsp: 0.728 ± 0.198
0.582CysGlu: 0.582 ± 0.207
0.291CysPhe: 0.291 ± 0.13
0.874CysGly: 0.874 ± 0.334
0.364CysHis: 0.364 ± 0.146
0.291CysIle: 0.291 ± 0.129
1.019CysLys: 1.019 ± 0.352
0.728CysLeu: 0.728 ± 0.272
0.218CysMet: 0.218 ± 0.139
0.51CysAsn: 0.51 ± 0.175
0.364CysPro: 0.364 ± 0.156
0.146CysGln: 0.146 ± 0.108
0.51CysArg: 0.51 ± 0.193
0.655CysSer: 0.655 ± 0.22
0.582CysThr: 0.582 ± 0.224
0.655CysVal: 0.655 ± 0.297
0.291CysTrp: 0.291 ± 0.14
0.146CysTyr: 0.146 ± 0.098
0.0CysXaa: 0.0 ± 0.0
Asp
4.15AspAla: 4.15 ± 0.515
0.801AspCys: 0.801 ± 0.246
3.567AspAsp: 3.567 ± 0.542
5.096AspGlu: 5.096 ± 0.564
2.475AspPhe: 2.475 ± 0.437
5.533AspGly: 5.533 ± 0.801
1.31AspHis: 1.31 ± 0.32
3.422AspIle: 3.422 ± 0.571
3.64AspLys: 3.64 ± 0.503
4.222AspLeu: 4.222 ± 0.452
1.31AspMet: 1.31 ± 0.243
2.402AspAsn: 2.402 ± 0.363
3.058AspPro: 3.058 ± 0.52
2.839AspGln: 2.839 ± 0.431
3.203AspArg: 3.203 ± 0.499
3.276AspSer: 3.276 ± 0.494
3.422AspThr: 3.422 ± 0.397
4.586AspVal: 4.586 ± 0.646
1.31AspTrp: 1.31 ± 0.272
1.238AspTyr: 1.238 ± 0.319
0.0AspXaa: 0.0 ± 0.0
Glu
7.207GluAla: 7.207 ± 0.938
0.437GluCys: 0.437 ± 0.167
4.295GluAsp: 4.295 ± 0.428
3.422GluGlu: 3.422 ± 0.632
2.621GluPhe: 2.621 ± 0.419
5.242GluGly: 5.242 ± 0.654
1.602GluHis: 1.602 ± 0.376
2.621GluIle: 2.621 ± 0.514
2.985GluLys: 2.985 ± 0.462
7.135GluLeu: 7.135 ± 0.774
1.238GluMet: 1.238 ± 0.327
1.82GluAsn: 1.82 ± 0.387
2.038GluPro: 2.038 ± 0.374
2.912GluGln: 2.912 ± 0.521
4.878GluArg: 4.878 ± 0.506
2.621GluSer: 2.621 ± 0.514
3.349GluThr: 3.349 ± 0.487
5.096GluVal: 5.096 ± 0.682
0.874GluTrp: 0.874 ± 0.262
2.038GluTyr: 2.038 ± 0.337
0.0GluXaa: 0.0 ± 0.0
Phe
2.475PheAla: 2.475 ± 0.479
0.364PheCys: 0.364 ± 0.163
2.111PheAsp: 2.111 ± 0.303
2.038PheGlu: 2.038 ± 0.367
1.82PhePhe: 1.82 ± 0.308
3.494PheGly: 3.494 ± 0.414
0.728PheHis: 0.728 ± 0.226
1.82PheIle: 1.82 ± 0.356
2.038PheLys: 2.038 ± 0.477
3.494PheLeu: 3.494 ± 0.446
0.728PheMet: 0.728 ± 0.221
2.257PheAsn: 2.257 ± 0.379
1.82PhePro: 1.82 ± 0.387
2.111PheGln: 2.111 ± 0.358
1.966PheArg: 1.966 ± 0.449
1.893PheSer: 1.893 ± 0.321
2.038PheThr: 2.038 ± 0.29
3.203PheVal: 3.203 ± 0.462
0.582PheTrp: 0.582 ± 0.211
0.801PheTyr: 0.801 ± 0.256
0.0PheXaa: 0.0 ± 0.0
Gly
7.79GlyAla: 7.79 ± 0.96
1.238GlyCys: 1.238 ± 0.333
4.586GlyAsp: 4.586 ± 0.602
4.514GlyGlu: 4.514 ± 0.53
2.33GlyPhe: 2.33 ± 0.46
6.479GlyGly: 6.479 ± 0.816
1.893GlyHis: 1.893 ± 0.384
4.368GlyIle: 4.368 ± 0.564
5.751GlyLys: 5.751 ± 0.678
6.479GlyLeu: 6.479 ± 0.776
2.257GlyMet: 2.257 ± 0.424
4.15GlyAsn: 4.15 ± 0.585
2.912GlyPro: 2.912 ± 0.528
4.15GlyGln: 4.15 ± 0.457
3.931GlyArg: 3.931 ± 0.487
6.989GlySer: 6.989 ± 0.786
5.46GlyThr: 5.46 ± 0.671
5.97GlyVal: 5.97 ± 0.652
1.238GlyTrp: 1.238 ± 0.28
2.33GlyTyr: 2.33 ± 0.346
0.0GlyXaa: 0.0 ± 0.0
His
1.092HisAla: 1.092 ± 0.297
0.364HisCys: 0.364 ± 0.194
0.874HisAsp: 0.874 ± 0.232
1.238HisGlu: 1.238 ± 0.308
0.801HisPhe: 0.801 ± 0.212
1.529HisGly: 1.529 ± 0.439
0.218HisHis: 0.218 ± 0.127
1.092HisIle: 1.092 ± 0.344
1.529HisLys: 1.529 ± 0.333
2.111HisLeu: 2.111 ± 0.458
0.364HisMet: 0.364 ± 0.144
0.801HisAsn: 0.801 ± 0.255
1.019HisPro: 1.019 ± 0.29
1.092HisGln: 1.092 ± 0.274
1.238HisArg: 1.238 ± 0.28
0.946HisSer: 0.946 ± 0.236
0.874HisThr: 0.874 ± 0.303
1.092HisVal: 1.092 ± 0.24
0.874HisTrp: 0.874 ± 0.329
0.874HisTyr: 0.874 ± 0.241
0.0HisXaa: 0.0 ± 0.0
Ile
3.64IleAla: 3.64 ± 0.624
0.218IleCys: 0.218 ± 0.151
3.058IleAsp: 3.058 ± 0.419
3.349IleGlu: 3.349 ± 0.473
1.456IlePhe: 1.456 ± 0.316
5.315IleGly: 5.315 ± 0.575
0.946IleHis: 0.946 ± 0.251
2.766IleIle: 2.766 ± 0.425
3.203IleLys: 3.203 ± 0.635
3.203IleLeu: 3.203 ± 0.52
1.529IleMet: 1.529 ± 0.28
2.038IleAsn: 2.038 ± 0.375
2.766IlePro: 2.766 ± 0.511
1.674IleGln: 1.674 ± 0.321
2.694IleArg: 2.694 ± 0.473
2.766IleSer: 2.766 ± 0.448
3.276IleThr: 3.276 ± 0.484
2.621IleVal: 2.621 ± 0.409
0.51IleTrp: 0.51 ± 0.205
1.383IleTyr: 1.383 ± 0.311
0.0IleXaa: 0.0 ± 0.0
Lys
6.407LysAla: 6.407 ± 0.833
0.364LysCys: 0.364 ± 0.206
4.004LysAsp: 4.004 ± 0.602
4.514LysGlu: 4.514 ± 0.397
2.33LysPhe: 2.33 ± 0.347
4.222LysGly: 4.222 ± 0.526
0.946LysHis: 0.946 ± 0.265
2.475LysIle: 2.475 ± 0.393
3.567LysLys: 3.567 ± 0.647
5.315LysLeu: 5.315 ± 0.678
1.31LysMet: 1.31 ± 0.307
2.839LysAsn: 2.839 ± 0.532
2.33LysPro: 2.33 ± 0.473
1.602LysGln: 1.602 ± 0.389
2.621LysArg: 2.621 ± 0.568
3.13LysSer: 3.13 ± 0.366
2.912LysThr: 2.912 ± 0.425
4.732LysVal: 4.732 ± 0.604
1.092LysTrp: 1.092 ± 0.287
2.621LysTyr: 2.621 ± 0.459
0.0LysXaa: 0.0 ± 0.0
Leu
8.081LeuAla: 8.081 ± 0.938
0.801LeuCys: 0.801 ± 0.231
5.169LeuAsp: 5.169 ± 0.54
4.586LeuGlu: 4.586 ± 0.608
3.276LeuPhe: 3.276 ± 0.517
6.188LeuGly: 6.188 ± 0.714
0.728LeuHis: 0.728 ± 0.227
4.368LeuIle: 4.368 ± 0.595
4.441LeuLys: 4.441 ± 0.467
6.771LeuLeu: 6.771 ± 0.824
2.257LeuMet: 2.257 ± 0.481
4.222LeuAsn: 4.222 ± 0.496
3.422LeuPro: 3.422 ± 0.556
3.494LeuGln: 3.494 ± 0.522
4.878LeuArg: 4.878 ± 0.72
4.15LeuSer: 4.15 ± 0.608
4.441LeuThr: 4.441 ± 0.562
5.46LeuVal: 5.46 ± 0.69
1.092LeuTrp: 1.092 ± 0.271
2.694LeuTyr: 2.694 ± 0.473
0.0LeuXaa: 0.0 ± 0.0
Met
2.621MetAla: 2.621 ± 0.353
0.51MetCys: 0.51 ± 0.224
1.238MetAsp: 1.238 ± 0.358
1.383MetGlu: 1.383 ± 0.377
0.801MetPhe: 0.801 ± 0.223
2.766MetGly: 2.766 ± 0.402
0.51MetHis: 0.51 ± 0.188
0.874MetIle: 0.874 ± 0.255
0.946MetLys: 0.946 ± 0.275
1.893MetLeu: 1.893 ± 0.337
0.728MetMet: 0.728 ± 0.2
1.383MetAsn: 1.383 ± 0.273
1.383MetPro: 1.383 ± 0.38
1.165MetGln: 1.165 ± 0.337
1.674MetArg: 1.674 ± 0.411
1.966MetSer: 1.966 ± 0.378
1.966MetThr: 1.966 ± 0.356
1.456MetVal: 1.456 ± 0.297
0.437MetTrp: 0.437 ± 0.156
1.238MetTyr: 1.238 ± 0.3
0.0MetXaa: 0.0 ± 0.0
Asn
3.858AsnAla: 3.858 ± 0.545
0.655AsnCys: 0.655 ± 0.221
2.621AsnAsp: 2.621 ± 0.433
2.257AsnGlu: 2.257 ± 0.495
1.893AsnPhe: 1.893 ± 0.41
3.786AsnGly: 3.786 ± 0.608
1.529AsnHis: 1.529 ± 0.495
3.494AsnIle: 3.494 ± 0.428
2.038AsnLys: 2.038 ± 0.437
3.13AsnLeu: 3.13 ± 0.457
1.529AsnMet: 1.529 ± 0.365
2.548AsnAsn: 2.548 ± 0.477
2.548AsnPro: 2.548 ± 0.426
2.038AsnGln: 2.038 ± 0.405
2.548AsnArg: 2.548 ± 0.39
1.602AsnSer: 1.602 ± 0.333
3.13AsnThr: 3.13 ± 0.47
2.694AsnVal: 2.694 ± 0.466
0.946AsnTrp: 0.946 ± 0.238
1.82AsnTyr: 1.82 ± 0.307
0.0AsnXaa: 0.0 ± 0.0
Pro
3.786ProAla: 3.786 ± 0.661
0.437ProCys: 0.437 ± 0.182
3.494ProAsp: 3.494 ± 0.655
4.441ProGlu: 4.441 ± 0.591
1.893ProPhe: 1.893 ± 0.26
2.766ProGly: 2.766 ± 0.476
0.946ProHis: 0.946 ± 0.282
1.529ProIle: 1.529 ± 0.389
2.839ProLys: 2.839 ± 0.431
2.766ProLeu: 2.766 ± 0.419
0.874ProMet: 0.874 ± 0.199
2.111ProAsn: 2.111 ± 0.501
1.82ProPro: 1.82 ± 0.657
2.548ProGln: 2.548 ± 0.447
1.82ProArg: 1.82 ± 0.404
3.13ProSer: 3.13 ± 0.529
2.621ProThr: 2.621 ± 0.598
2.839ProVal: 2.839 ± 0.48
0.437ProTrp: 0.437 ± 0.135
1.747ProTyr: 1.747 ± 0.462
0.0ProXaa: 0.0 ± 0.0
Gln
4.077GlnAla: 4.077 ± 0.618
0.218GlnCys: 0.218 ± 0.13
2.621GlnAsp: 2.621 ± 0.508
3.567GlnGlu: 3.567 ± 0.474
1.383GlnPhe: 1.383 ± 0.271
3.494GlnGly: 3.494 ± 0.606
0.582GlnHis: 0.582 ± 0.22
1.966GlnIle: 1.966 ± 0.328
2.038GlnLys: 2.038 ± 0.361
4.077GlnLeu: 4.077 ± 0.413
1.529GlnMet: 1.529 ± 0.416
2.184GlnAsn: 2.184 ± 0.523
1.529GlnPro: 1.529 ± 0.331
2.402GlnGln: 2.402 ± 0.553
2.402GlnArg: 2.402 ± 0.453
2.694GlnSer: 2.694 ± 0.436
2.621GlnThr: 2.621 ± 0.481
3.203GlnVal: 3.203 ± 0.515
0.582GlnTrp: 0.582 ± 0.245
1.019GlnTyr: 1.019 ± 0.285
0.0GlnXaa: 0.0 ± 0.0
Arg
4.514ArgAla: 4.514 ± 0.591
0.582ArgCys: 0.582 ± 0.232
3.349ArgAsp: 3.349 ± 0.489
3.276ArgGlu: 3.276 ± 0.521
2.111ArgPhe: 2.111 ± 0.373
3.713ArgGly: 3.713 ± 0.468
1.092ArgHis: 1.092 ± 0.286
3.494ArgIle: 3.494 ± 0.536
4.222ArgLys: 4.222 ± 0.648
3.713ArgLeu: 3.713 ± 0.525
2.184ArgMet: 2.184 ± 0.38
2.475ArgAsn: 2.475 ± 0.428
2.548ArgPro: 2.548 ± 0.464
2.839ArgGln: 2.839 ± 0.57
3.13ArgArg: 3.13 ± 0.431
2.257ArgSer: 2.257 ± 0.482
2.621ArgThr: 2.621 ± 0.507
3.494ArgVal: 3.494 ± 0.5
0.51ArgTrp: 0.51 ± 0.17
1.674ArgTyr: 1.674 ± 0.315
0.0ArgXaa: 0.0 ± 0.0
Ser
3.858SerAla: 3.858 ± 0.661
0.582SerCys: 0.582 ± 0.213
3.058SerAsp: 3.058 ± 0.533
3.203SerGlu: 3.203 ± 0.459
2.038SerPhe: 2.038 ± 0.418
4.805SerGly: 4.805 ± 0.614
1.529SerHis: 1.529 ± 0.293
2.548SerIle: 2.548 ± 0.359
3.276SerLys: 3.276 ± 0.478
5.242SerLeu: 5.242 ± 0.643
1.165SerMet: 1.165 ± 0.283
2.548SerAsn: 2.548 ± 0.454
2.257SerPro: 2.257 ± 0.507
1.966SerGln: 1.966 ± 0.301
3.276SerArg: 3.276 ± 0.427
3.422SerSer: 3.422 ± 0.729
2.257SerThr: 2.257 ± 0.394
4.077SerVal: 4.077 ± 0.487
0.874SerTrp: 0.874 ± 0.276
1.82SerTyr: 1.82 ± 0.402
0.0SerXaa: 0.0 ± 0.0
Thr
5.751ThrAla: 5.751 ± 0.679
0.146ThrCys: 0.146 ± 0.086
2.621ThrAsp: 2.621 ± 0.346
3.567ThrGlu: 3.567 ± 0.481
2.402ThrPhe: 2.402 ± 0.392
5.897ThrGly: 5.897 ± 0.72
1.383ThrHis: 1.383 ± 0.352
2.257ThrIle: 2.257 ± 0.513
2.839ThrLys: 2.839 ± 0.499
4.878ThrLeu: 4.878 ± 0.531
1.966ThrMet: 1.966 ± 0.396
1.893ThrAsn: 1.893 ± 0.357
3.713ThrPro: 3.713 ± 0.552
2.257ThrGln: 2.257 ± 0.377
2.475ThrArg: 2.475 ± 0.471
3.058ThrSer: 3.058 ± 0.516
2.694ThrThr: 2.694 ± 0.537
4.004ThrVal: 4.004 ± 0.381
0.946ThrTrp: 0.946 ± 0.275
1.092ThrTyr: 1.092 ± 0.318
0.0ThrXaa: 0.0 ± 0.0
Val
5.533ValAla: 5.533 ± 0.622
0.582ValCys: 0.582 ± 0.185
5.169ValAsp: 5.169 ± 0.63
4.586ValGlu: 4.586 ± 0.577
2.766ValPhe: 2.766 ± 0.475
5.897ValGly: 5.897 ± 0.682
1.383ValHis: 1.383 ± 0.409
2.766ValIle: 2.766 ± 0.491
3.713ValLys: 3.713 ± 0.494
5.096ValLeu: 5.096 ± 0.706
2.111ValMet: 2.111 ± 0.421
4.441ValAsn: 4.441 ± 0.628
3.13ValPro: 3.13 ± 0.368
3.058ValGln: 3.058 ± 0.484
4.295ValArg: 4.295 ± 0.519
2.839ValSer: 2.839 ± 0.384
3.858ValThr: 3.858 ± 0.618
5.97ValVal: 5.97 ± 0.687
1.31ValTrp: 1.31 ± 0.301
2.621ValTyr: 2.621 ± 0.46
0.0ValXaa: 0.0 ± 0.0
Trp
1.529TrpAla: 1.529 ± 0.327
0.146TrpCys: 0.146 ± 0.154
1.602TrpAsp: 1.602 ± 0.296
0.874TrpGlu: 0.874 ± 0.206
0.728TrpPhe: 0.728 ± 0.215
0.946TrpGly: 0.946 ± 0.265
0.146TrpHis: 0.146 ± 0.106
0.364TrpIle: 0.364 ± 0.149
1.383TrpLys: 1.383 ± 0.302
1.383TrpLeu: 1.383 ± 0.367
0.364TrpMet: 0.364 ± 0.142
0.51TrpAsn: 0.51 ± 0.167
0.364TrpPro: 0.364 ± 0.225
0.437TrpGln: 0.437 ± 0.151
1.31TrpArg: 1.31 ± 0.309
1.092TrpSer: 1.092 ± 0.355
0.655TrpThr: 0.655 ± 0.207
1.31TrpVal: 1.31 ± 0.302
0.146TrpTrp: 0.146 ± 0.09
0.51TrpTyr: 0.51 ± 0.227
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.82TyrAla: 1.82 ± 0.344
0.582TyrCys: 0.582 ± 0.202
2.184TyrAsp: 2.184 ± 0.433
1.674TyrGlu: 1.674 ± 0.328
1.456TyrPhe: 1.456 ± 0.297
2.548TyrGly: 2.548 ± 0.373
0.728TyrHis: 0.728 ± 0.252
1.747TyrIle: 1.747 ± 0.423
2.038TyrLys: 2.038 ± 0.351
2.475TyrLeu: 2.475 ± 0.462
0.728TyrMet: 0.728 ± 0.185
2.038TyrAsn: 2.038 ± 0.35
1.747TyrPro: 1.747 ± 0.403
1.383TyrGln: 1.383 ± 0.304
1.602TyrArg: 1.602 ± 0.347
1.165TyrSer: 1.165 ± 0.265
2.184TyrThr: 2.184 ± 0.362
1.966TyrVal: 1.966 ± 0.361
0.582TyrTrp: 0.582 ± 0.191
1.165TyrTyr: 1.165 ± 0.342
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (13737 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski