Amino acid dipepetide frequency for Pseudonocardia asaccharolytica DSM 44247 = NBRC 16224

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.65AlaAla: 23.65 ± 0.239	1.153AlaCys: 1.153 ± 0.031	8.571AlaAsp: 8.571 ± 0.092	9.439AlaGlu: 9.439 ± 0.096	3.343AlaPhe: 3.343 ± 0.049	14.158AlaGly: 14.158 ± 0.125	2.886AlaHis: 2.886 ± 0.05	4.264AlaIle: 4.264 ± 0.062	2.113AlaLys: 2.113 ± 0.047	14.635AlaLeu: 14.635 ± 0.128	2.681AlaMet: 2.681 ± 0.043	1.901AlaAsn: 1.901 ± 0.039	7.021AlaPro: 7.021 ± 0.085	3.757AlaGln: 3.757 ± 0.055	11.126AlaArg: 11.126 ± 0.119	5.261AlaSer: 5.261 ± 0.062	7.129AlaThr: 7.129 ± 0.078	12.957AlaVal: 12.957 ± 0.115	1.775AlaTrp: 1.775 ± 0.038	2.102AlaTyr: 2.102 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	1.159CysAla: 1.159 ± 0.031	0.125CysCys: 0.125 ± 0.009	0.517CysAsp: 0.517 ± 0.02	0.453CysGlu: 0.453 ± 0.018	0.217CysPhe: 0.217 ± 0.013	1.103CysGly: 1.103 ± 0.028	0.213CysHis: 0.213 ± 0.013	0.222CysIle: 0.222 ± 0.013	0.101CysLys: 0.101 ± 0.008	0.76CysLeu: 0.76 ± 0.02	0.129CysMet: 0.129 ± 0.009	0.135CysAsn: 0.135 ± 0.01	0.559CysPro: 0.559 ± 0.025	0.191CysGln: 0.191 ± 0.01	0.79CysArg: 0.79 ± 0.027	0.486CysSer: 0.486 ± 0.016	0.532CysThr: 0.532 ± 0.024	0.67CysVal: 0.67 ± 0.022	0.142CysTrp: 0.142 ± 0.01	0.205CysTyr: 0.205 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	7.942AspAla: 7.942 ± 0.089	0.426AspCys: 0.426 ± 0.018	3.584AspAsp: 3.584 ± 0.053	4.137AspGlu: 4.137 ± 0.062	1.374AspPhe: 1.374 ± 0.034	6.166AspGly: 6.166 ± 0.065	1.372AspHis: 1.372 ± 0.029	1.838AspIle: 1.838 ± 0.038	0.829AspLys: 0.829 ± 0.028	6.323AspLeu: 6.323 ± 0.07	0.764AspMet: 0.764 ± 0.023	0.779AspAsn: 0.779 ± 0.024	5.067AspPro: 5.067 ± 0.057	1.517AspGln: 1.517 ± 0.034	5.337AspArg: 5.337 ± 0.057	2.037AspSer: 2.037 ± 0.038	2.778AspThr: 2.778 ± 0.039	5.332AspVal: 5.332 ± 0.062	0.846AspTrp: 0.846 ± 0.026	1.093AspTyr: 1.093 ± 0.027	0.0AspXaa: 0.0 ± 0.0
Glu	6.695GluAla: 6.695 ± 0.072	0.389GluCys: 0.389 ± 0.017	2.452GluAsp: 2.452 ± 0.044	2.613GluGlu: 2.613 ± 0.051	1.619GluPhe: 1.619 ± 0.031	3.668GluGly: 3.668 ± 0.05	1.696GluHis: 1.696 ± 0.034	2.675GluIle: 2.675 ± 0.048	1.056GluLys: 1.056 ± 0.033	7.491GluLeu: 7.491 ± 0.082	0.934GluMet: 0.934 ± 0.028	0.922GluAsn: 0.922 ± 0.026	3.686GluPro: 3.686 ± 0.056	2.424GluGln: 2.424 ± 0.044	5.697GluArg: 5.697 ± 0.065	2.239GluSer: 2.239 ± 0.036	2.742GluThr: 2.742 ± 0.04	4.871GluVal: 4.871 ± 0.059	0.797GluTrp: 0.797 ± 0.022	0.984GluTyr: 0.984 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	3.803PheAla: 3.803 ± 0.05	0.298PheCys: 0.298 ± 0.015	2.065PheAsp: 2.065 ± 0.041	1.285PheGlu: 1.285 ± 0.029	0.785PhePhe: 0.785 ± 0.024	3.11PheGly: 3.11 ± 0.048	0.581PheHis: 0.581 ± 0.021	0.693PheIle: 0.693 ± 0.024	0.326PheLys: 0.326 ± 0.017	2.444PheLeu: 2.444 ± 0.04	0.347PheMet: 0.347 ± 0.015	0.512PheAsn: 0.512 ± 0.019	1.358PhePro: 1.358 ± 0.031	0.551PheGln: 0.551 ± 0.02	1.858PheArg: 1.858 ± 0.038	1.329PheSer: 1.329 ± 0.03	1.785PheThr: 1.785 ± 0.035	2.344PheVal: 2.344 ± 0.04	0.413PheTrp: 0.413 ± 0.018	0.558PheTyr: 0.558 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	11.208GlyAla: 11.208 ± 0.094	0.98GlyCys: 0.98 ± 0.031	4.898GlyAsp: 4.898 ± 0.06	5.089GlyGlu: 5.089 ± 0.055	2.861GlyPhe: 2.861 ± 0.042	8.596GlyGly: 8.596 ± 0.107	2.243GlyHis: 2.243 ± 0.046	3.689GlyIle: 3.689 ± 0.052	1.804GlyLys: 1.804 ± 0.034	9.976GlyLeu: 9.976 ± 0.108	2.196GlyMet: 2.196 ± 0.04	1.559GlyAsn: 1.559 ± 0.033	5.595GlyPro: 5.595 ± 0.075	2.536GlyGln: 2.536 ± 0.047	8.48GlyArg: 8.48 ± 0.076	5.239GlySer: 5.239 ± 0.065	5.473GlyThr: 5.473 ± 0.066	8.116GlyVal: 8.116 ± 0.073	1.845GlyTrp: 1.845 ± 0.039	2.335GlyTyr: 2.335 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.694HisAla: 2.694 ± 0.051	0.225HisCys: 0.225 ± 0.012	1.353HisAsp: 1.353 ± 0.032	1.212HisGlu: 1.212 ± 0.03	0.569HisPhe: 0.569 ± 0.022	2.412HisGly: 2.412 ± 0.047	0.699HisHis: 0.699 ± 0.026	0.624HisIle: 0.624 ± 0.019	0.278HisLys: 0.278 ± 0.015	2.397HisLeu: 2.397 ± 0.047	0.289HisMet: 0.289 ± 0.014	0.397HisAsn: 0.397 ± 0.016	1.954HisPro: 1.954 ± 0.036	0.586HisGln: 0.586 ± 0.018	2.306HisArg: 2.306 ± 0.039	0.966HisSer: 0.966 ± 0.024	1.143HisThr: 1.143 ± 0.028	1.803HisVal: 1.803 ± 0.036	0.343HisTrp: 0.343 ± 0.016	0.482HisTyr: 0.482 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.308IleAla: 5.308 ± 0.064	0.332IleCys: 0.332 ± 0.014	2.739IleAsp: 2.739 ± 0.048	2.26IleGlu: 2.26 ± 0.043	0.751IlePhe: 0.751 ± 0.024	4.091IleGly: 4.091 ± 0.061	0.646IleHis: 0.646 ± 0.021	1.021IleIle: 1.021 ± 0.033	0.609IleLys: 0.609 ± 0.022	2.424IleLeu: 2.424 ± 0.046	0.443IleMet: 0.443 ± 0.02	0.769IleAsn: 0.769 ± 0.023	1.987IlePro: 1.987 ± 0.036	0.728IleGln: 0.728 ± 0.019	2.57IleArg: 2.57 ± 0.043	1.697IleSer: 1.697 ± 0.038	2.295IleThr: 2.295 ± 0.041	3.328IleVal: 3.328 ± 0.05	0.447IleTrp: 0.447 ± 0.018	0.651IleTyr: 0.651 ± 0.021	0.0IleXaa: 0.0 ± 0.0
Lys	2.007LysAla: 2.007 ± 0.038	0.097LysCys: 0.097 ± 0.009	0.756LysAsp: 0.756 ± 0.024	0.739LysGlu: 0.739 ± 0.029	0.35LysPhe: 0.35 ± 0.018	1.198LysGly: 1.198 ± 0.035	0.33LysHis: 0.33 ± 0.015	0.846LysIle: 0.846 ± 0.027	0.376LysLys: 0.376 ± 0.022	1.548LysLeu: 1.548 ± 0.033	0.294LysMet: 0.294 ± 0.016	0.318LysAsn: 0.318 ± 0.016	1.006LysPro: 1.006 ± 0.03	0.547LysGln: 0.547 ± 0.022	1.243LysArg: 1.243 ± 0.034	0.716LysSer: 0.716 ± 0.023	0.995LysThr: 0.995 ± 0.026	1.424LysVal: 1.424 ± 0.037	0.175LysTrp: 0.175 ± 0.011	0.306LysTyr: 0.306 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	16.565LeuAla: 16.565 ± 0.146	0.859LeuCys: 0.859 ± 0.025	7.114LeuAsp: 7.114 ± 0.077	4.808LeuGlu: 4.808 ± 0.068	2.547LeuPhe: 2.547 ± 0.051	9.923LeuGly: 9.923 ± 0.1	2.36LeuHis: 2.36 ± 0.045	3.372LeuIle: 3.372 ± 0.051	1.239LeuLys: 1.239 ± 0.036	11.59LeuLeu: 11.59 ± 0.103	1.404LeuMet: 1.404 ± 0.032	1.457LeuAsn: 1.457 ± 0.032	6.46LeuPro: 6.46 ± 0.072	2.205LeuGln: 2.205 ± 0.038	9.553LeuArg: 9.553 ± 0.091	4.842LeuSer: 4.842 ± 0.066	6.152LeuThr: 6.152 ± 0.074	10.067LeuVal: 10.067 ± 0.107	1.197LeuTrp: 1.197 ± 0.037	1.57LeuTyr: 1.57 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	2.335MetAla: 2.335 ± 0.041	0.136MetCys: 0.136 ± 0.011	0.798MetAsp: 0.798 ± 0.025	0.656MetGlu: 0.656 ± 0.024	0.459MetPhe: 0.459 ± 0.019	1.257MetGly: 1.257 ± 0.032	0.393MetHis: 0.393 ± 0.016	0.813MetIle: 0.813 ± 0.026	0.315MetLys: 0.315 ± 0.014	1.883MetLeu: 1.883 ± 0.038	0.275MetMet: 0.275 ± 0.014	0.412MetAsn: 0.412 ± 0.017	1.139MetPro: 1.139 ± 0.028	0.426MetGln: 0.426 ± 0.015	1.541MetArg: 1.541 ± 0.035	1.264MetSer: 1.264 ± 0.031	1.522MetThr: 1.522 ± 0.034	1.395MetVal: 1.395 ± 0.033	0.168MetTrp: 0.168 ± 0.011	0.242MetTyr: 0.242 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.057AsnAla: 2.057 ± 0.035	0.145AsnCys: 0.145 ± 0.01	0.787AsnAsp: 0.787 ± 0.025	0.707AsnGlu: 0.707 ± 0.023	0.43AsnPhe: 0.43 ± 0.017	1.617AsnGly: 1.617 ± 0.036	0.361AsnHis: 0.361 ± 0.015	0.608AsnIle: 0.608 ± 0.022	0.296AsnLys: 0.296 ± 0.016	1.67AsnLeu: 1.67 ± 0.033	0.258AsnMet: 0.258 ± 0.014	0.311AsnAsn: 0.311 ± 0.016	1.455AsnPro: 1.455 ± 0.034	0.442AsnGln: 0.442 ± 0.016	1.316AsnArg: 1.316 ± 0.029	0.712AsnSer: 0.712 ± 0.02	0.859AsnThr: 0.859 ± 0.027	1.346AsnVal: 1.346 ± 0.029	0.261AsnTrp: 0.261 ± 0.016	0.37AsnTyr: 0.37 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	9.079ProAla: 9.079 ± 0.104	0.396ProCys: 0.396 ± 0.017	4.88ProAsp: 4.88 ± 0.06	4.11ProGlu: 4.11 ± 0.056	1.509ProPhe: 1.509 ± 0.033	7.083ProGly: 7.083 ± 0.086	1.286ProHis: 1.286 ± 0.031	1.907ProIle: 1.907 ± 0.035	0.86ProLys: 0.86 ± 0.025	5.32ProLeu: 5.32 ± 0.062	1.152ProMet: 1.152 ± 0.029	0.921ProAsn: 0.921 ± 0.03	4.121ProPro: 4.121 ± 0.073	1.597ProGln: 1.597 ± 0.035	4.504ProArg: 4.504 ± 0.064	3.01ProSer: 3.01 ± 0.045	3.461ProThr: 3.461 ± 0.056	5.83ProVal: 5.83 ± 0.069	0.915ProTrp: 0.915 ± 0.03	1.052ProTyr: 1.052 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	3.538GlnAla: 3.538 ± 0.053	0.183GlnCys: 0.183 ± 0.012	1.074GlnAsp: 1.074 ± 0.031	1.116GlnGlu: 1.116 ± 0.029	0.676GlnPhe: 0.676 ± 0.02	1.793GlnGly: 1.793 ± 0.035	0.66GlnHis: 0.66 ± 0.024	1.198GlnIle: 1.198 ± 0.032	0.434GlnLys: 0.434 ± 0.02	3.3GlnLeu: 3.3 ± 0.049	0.48GlnMet: 0.48 ± 0.018	0.443GlnAsn: 0.443 ± 0.02	1.743GlnPro: 1.743 ± 0.038	1.063GlnGln: 1.063 ± 0.034	2.755GlnArg: 2.755 ± 0.046	1.007GlnSer: 1.007 ± 0.029	1.346GlnThr: 1.346 ± 0.028	2.631GlnVal: 2.631 ± 0.04	0.358GlnTrp: 0.358 ± 0.015	0.484GlnTyr: 0.484 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	11.026ArgAla: 11.026 ± 0.106	0.848ArgCys: 0.848 ± 0.028	4.641ArgAsp: 4.641 ± 0.059	4.67ArgGlu: 4.67 ± 0.062	2.62ArgPhe: 2.62 ± 0.049	6.747ArgGly: 6.747 ± 0.067	2.21ArgHis: 2.21 ± 0.039	3.548ArgIle: 3.548 ± 0.049	1.286ArgLys: 1.286 ± 0.033	9.402ArgLeu: 9.402 ± 0.106	1.992ArgMet: 1.992 ± 0.034	1.426ArgAsn: 1.426 ± 0.032	5.627ArgPro: 5.627 ± 0.075	2.244ArgGln: 2.244 ± 0.039	9.51ArgArg: 9.51 ± 0.107	4.603ArgSer: 4.603 ± 0.063	4.932ArgThr: 4.932 ± 0.06	6.625ArgVal: 6.625 ± 0.069	1.576ArgTrp: 1.576 ± 0.036	2.028ArgTyr: 2.028 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	6.172SerAla: 6.172 ± 0.072	0.443SerCys: 0.443 ± 0.018	2.498SerAsp: 2.498 ± 0.044	2.207SerGlu: 2.207 ± 0.039	1.381SerPhe: 1.381 ± 0.029	5.384SerGly: 5.384 ± 0.057	0.831SerHis: 0.831 ± 0.024	1.632SerIle: 1.632 ± 0.037	0.71SerLys: 0.71 ± 0.022	4.178SerLeu: 4.178 ± 0.055	1.095SerMet: 1.095 ± 0.027	0.761SerAsn: 0.761 ± 0.025	3.049SerPro: 3.049 ± 0.051	1.063SerGln: 1.063 ± 0.025	3.89SerArg: 3.89 ± 0.049	2.517SerSer: 2.517 ± 0.049	2.865SerThr: 2.865 ± 0.047	3.963SerVal: 3.963 ± 0.058	0.859SerTrp: 0.859 ± 0.023	1.015SerTyr: 1.015 ± 0.029	0.0SerXaa: 0.0 ± 0.0
Thr	7.929ThrAla: 7.929 ± 0.073	0.454ThrCys: 0.454 ± 0.018	3.348ThrAsp: 3.348 ± 0.05	3.181ThrGlu: 3.181 ± 0.048	1.513ThrPhe: 1.513 ± 0.032	6.2ThrGly: 6.2 ± 0.068	1.147ThrHis: 1.147 ± 0.032	2.025ThrIle: 2.025 ± 0.042	0.852ThrLys: 0.852 ± 0.027	5.269ThrLeu: 5.269 ± 0.061	0.995ThrMet: 0.995 ± 0.028	0.924ThrAsn: 0.924 ± 0.028	3.69ThrPro: 3.69 ± 0.058	1.254ThrGln: 1.254 ± 0.029	4.087ThrArg: 4.087 ± 0.059	2.717ThrSer: 2.717 ± 0.048	3.437ThrThr: 3.437 ± 0.058	5.702ThrVal: 5.702 ± 0.066	0.8ThrTrp: 0.8 ± 0.024	1.032ThrTyr: 1.032 ± 0.026	0.0ThrXaa: 0.0 ± 0.0
Val	12.709ValAla: 12.709 ± 0.133	0.845ValCys: 0.845 ± 0.026	5.527ValAsp: 5.527 ± 0.063	5.299ValGlu: 5.299 ± 0.067	2.417ValPhe: 2.417 ± 0.04	7.398ValGly: 7.398 ± 0.077	2.054ValHis: 2.054 ± 0.037	3.147ValIle: 3.147 ± 0.051	1.243ValLys: 1.243 ± 0.032	10.644ValLeu: 10.644 ± 0.099	1.218ValMet: 1.218 ± 0.031	1.466ValAsn: 1.466 ± 0.032	5.449ValPro: 5.449 ± 0.06	2.046ValGln: 2.046 ± 0.03	7.633ValArg: 7.633 ± 0.077	4.161ValSer: 4.161 ± 0.054	5.204ValThr: 5.204 ± 0.06	8.986ValVal: 8.986 ± 0.097	1.081ValTrp: 1.081 ± 0.031	1.439ValTyr: 1.439 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.716TrpAla: 1.716 ± 0.037	0.176TrpCys: 0.176 ± 0.012	0.725TrpAsp: 0.725 ± 0.024	0.698TrpGlu: 0.698 ± 0.024	0.467TrpPhe: 0.467 ± 0.019	0.932TrpGly: 0.932 ± 0.026	0.354TrpHis: 0.354 ± 0.016	0.525TrpIle: 0.525 ± 0.023	0.261TrpLys: 0.261 ± 0.015	1.866TrpLeu: 1.866 ± 0.039	0.285TrpMet: 0.285 ± 0.015	0.307TrpAsn: 0.307 ± 0.014	0.873TrpPro: 0.873 ± 0.025	0.523TrpGln: 0.523 ± 0.018	1.387TrpArg: 1.387 ± 0.032	0.891TrpSer: 0.891 ± 0.024	0.916TrpThr: 0.916 ± 0.023	1.096TrpVal: 1.096 ± 0.028	0.35TrpTrp: 0.35 ± 0.015	0.306TrpTyr: 0.306 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.33TyrAla: 2.33 ± 0.042	0.196TyrCys: 0.196 ± 0.013	1.214TyrAsp: 1.214 ± 0.034	0.973TyrGlu: 0.973 ± 0.026	0.556TyrPhe: 0.556 ± 0.023	1.886TyrGly: 1.886 ± 0.04	0.412TyrHis: 0.412 ± 0.019	0.449TyrIle: 0.449 ± 0.019	0.283TyrLys: 0.283 ± 0.013	2.163TyrLeu: 2.163 ± 0.041	0.198TyrMet: 0.198 ± 0.012	0.288TyrAsn: 0.288 ± 0.013	1.153TyrPro: 1.153 ± 0.031	0.58TyrGln: 0.58 ± 0.022	1.975TyrArg: 1.975 ± 0.042	0.819TyrSer: 0.819 ± 0.023	0.991TyrThr: 0.991 ± 0.026	1.482TyrVal: 1.482 ± 0.034	0.307TyrTrp: 0.307 ± 0.016	0.419TyrTyr: 0.419 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4883 proteins (1461661 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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