Pseudodesulfovibrio cashew
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3498 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I6JM85|A0A6I6JM85_9DELT DNA helicase OS=Pseudodesulfovibrio cashew OX=2678688 GN=GM415_15175 PE=4 SV=1
MM1 pKa = 7.63 YY2 pKa = 7.91 DD3 pKa = 3.36 TLSFATSNNSPTSVTMSLSLEE24 pKa = 4.15 GLLSNTSPDD33 pKa = 3.31 GYY35 pKa = 9.91 ATSDD39 pKa = 3.46 TSISIYY45 pKa = 10.52 DD46 pKa = 3.32 ITGYY50 pKa = 10.48 SSWLEE55 pKa = 3.61 EE56 pKa = 4.0 SAYY59 pKa = 10.23 GLGMVTSFTKK69 pKa = 10.68 VATAAINFAVGDD81 pKa = 4.08 QYY83 pKa = 11.64 WLDD86 pKa = 4.88 LLNQWVSFDD95 pKa = 3.66 DD96 pKa = 3.64 MAYY99 pKa = 8.45 DD100 pKa = 3.85 TTGQTYY106 pKa = 10.76 SFDD109 pKa = 3.61 LTKK112 pKa = 10.91 SLTFEE117 pKa = 4.73 ADD119 pKa = 3.04 PLKK122 pKa = 10.31 TYY124 pKa = 10.07 GIRR127 pKa = 11.84 IWTSADD133 pKa = 3.13 ANGSNEE139 pKa = 4.57 GISASDD145 pKa = 3.9 FYY147 pKa = 11.02 NTSEE151 pKa = 4.67 VGFTEE156 pKa = 4.84 LNGATFEE163 pKa = 4.38 SGSGAFLANQGSTAPVATPEE183 pKa = 4.14 PSTILLLGAGLFGLVAVRR201 pKa = 11.84 RR202 pKa = 11.84 KK203 pKa = 9.63 RR204 pKa = 11.84 AAIQQ208 pKa = 3.14
Molecular weight: 22.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.872
IPC_protein 3.834
Toseland 3.617
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.757
Rodwell 3.668
Grimsley 3.541
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.164
Thurlkill 3.681
EMBOSS 3.77
Sillero 3.948
Patrickios 0.947
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.863
Protein with the highest isoelectric point:
>tr|A0A6I6JGK3|A0A6I6JGK3_9DELT Ppx-GppA domain-containing protein OS=Pseudodesulfovibrio cashew OX=2678688 GN=GM415_08410 PE=4 SV=1
MM1 pKa = 7.7 PKK3 pKa = 9.95 IKK5 pKa = 9.47 TRR7 pKa = 11.84 RR8 pKa = 11.84 AAAKK12 pKa = 9.74 RR13 pKa = 11.84 FSKK16 pKa = 9.7 TATGKK21 pKa = 10.1 FKK23 pKa = 10.74 RR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 KK27 pKa = 9.07 NLRR30 pKa = 11.84 HH31 pKa = 6.24 ILTKK35 pKa = 10.67 KK36 pKa = 7.68 NAKK39 pKa = 9.22 RR40 pKa = 11.84 KK41 pKa = 9.18 RR42 pKa = 11.84 RR43 pKa = 11.84 LGQSTTVDD51 pKa = 3.58 GANMKK56 pKa = 10.1 AVRR59 pKa = 11.84 RR60 pKa = 11.84 QLPNGG65 pKa = 3.51
Molecular weight: 7.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 10.979
IPC_protein 12.544
Toseland 12.72
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.544
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.266
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.04
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3498
0
3498
1141533
33
3767
326.3
35.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.641 ± 0.048
1.306 ± 0.017
5.801 ± 0.04
6.808 ± 0.044
4.096 ± 0.027
8.224 ± 0.04
2.001 ± 0.017
5.376 ± 0.034
4.815 ± 0.04
10.343 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.934 ± 0.019
3.081 ± 0.021
4.637 ± 0.029
2.927 ± 0.022
6.03 ± 0.046
5.508 ± 0.031
5.143 ± 0.038
7.41 ± 0.035
1.156 ± 0.015
2.764 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here