Pseudogymnoascus sp. VKM F-3808

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus; unclassified Pseudogymnoascus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9928 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A093ZVJ7|A0A093ZVJ7_9PEZI Uncharacterized protein OS=Pseudogymnoascus sp. VKM F-3808 OX=1391699 GN=O988_08502 PE=4 SV=1
MM1 pKa = 7.44AEE3 pKa = 4.81KK4 pKa = 10.98YY5 pKa = 10.37PFDD8 pKa = 4.34LQTPQPKK15 pKa = 10.17YY16 pKa = 10.86DD17 pKa = 4.58DD18 pKa = 4.83YY19 pKa = 11.98YY20 pKa = 11.61DD21 pKa = 4.8DD22 pKa = 5.46DD23 pKa = 6.13SYY25 pKa = 12.09DD26 pKa = 3.57GSSSDD31 pKa = 5.34DD32 pKa = 3.66SSDD35 pKa = 3.91DD36 pKa = 4.15DD37 pKa = 5.33SSDD40 pKa = 3.5EE41 pKa = 4.25YY42 pKa = 11.78GSFDD46 pKa = 4.03YY47 pKa = 11.29DD48 pKa = 5.61DD49 pKa = 4.91IYY51 pKa = 11.45EE52 pKa = 4.88DD53 pKa = 5.54DD54 pKa = 5.61DD55 pKa = 4.73DD56 pKa = 5.91FSDD59 pKa = 3.92EE60 pKa = 4.03RR61 pKa = 11.84TIPKK65 pKa = 9.03TNPILAYY72 pKa = 9.05YY73 pKa = 9.91GIVRR77 pKa = 11.84EE78 pKa = 4.04VRR80 pKa = 11.84KK81 pKa = 9.94AVVDD85 pKa = 3.3IAIICVSLWCIWNFDD100 pKa = 3.77FFVDD104 pKa = 3.64LMQRR108 pKa = 11.84ILSGEE113 pKa = 4.12SPKK116 pKa = 10.58VSLVYY121 pKa = 9.48PAITLTPSSKK131 pKa = 10.47LSSIKK136 pKa = 10.24LLHH139 pKa = 6.84PSNINPPPTINHH151 pKa = 6.56PPP153 pKa = 3.21

Molecular weight: 17.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A093ZPV1|A0A093ZPV1_9PEZI Uncharacterized protein OS=Pseudogymnoascus sp. VKM F-3808 OX=1391699 GN=O988_09310 PE=4 SV=1
MM1 pKa = 7.26GMGMGWRR8 pKa = 11.84GWGVRR13 pKa = 11.84RR14 pKa = 11.84KK15 pKa = 8.97MGRR18 pKa = 11.84ISPTPTPPASKK29 pKa = 9.94QSKK32 pKa = 8.57PSSSSSQSAPGPPASSSANPSPSAAGSPNSSKK64 pKa = 10.94NPASPTPPPLTPRR77 pKa = 11.84SPGKK81 pKa = 10.12NGPASRR87 pKa = 11.84ARR89 pKa = 11.84SARR92 pKa = 11.84NSS94 pKa = 3.24

Molecular weight: 9.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9928

0

9928

4665772

11

5521

470.0

51.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.932 ± 0.023

1.211 ± 0.012

5.646 ± 0.019

6.292 ± 0.027

3.633 ± 0.016

7.214 ± 0.022

2.244 ± 0.01

5.062 ± 0.018

5.05 ± 0.023

8.729 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.154 ± 0.01

3.743 ± 0.014

5.923 ± 0.026

3.851 ± 0.018

5.802 ± 0.021

8.119 ± 0.025

6.056 ± 0.014

6.152 ± 0.017

1.415 ± 0.01

2.773 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski