Amino acid dipepetide frequency for Pseudogymnoascus sp. VKM F-3808

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.915AlaAla: 9.915 ± 0.08	1.072AlaCys: 1.072 ± 0.016	4.445AlaAsp: 4.445 ± 0.035	5.448AlaGlu: 5.448 ± 0.039	3.108AlaPhe: 3.108 ± 0.03	6.248AlaGly: 6.248 ± 0.044	1.729AlaHis: 1.729 ± 0.021	4.556AlaIle: 4.556 ± 0.035	4.5AlaLys: 4.5 ± 0.042	7.891AlaLeu: 7.891 ± 0.051	2.026AlaMet: 2.026 ± 0.024	3.102AlaAsn: 3.102 ± 0.025	5.203AlaPro: 5.203 ± 0.046	3.414AlaGln: 3.414 ± 0.03	4.815AlaArg: 4.815 ± 0.038	7.293AlaSer: 7.293 ± 0.049	5.616AlaThr: 5.616 ± 0.044	5.58AlaVal: 5.58 ± 0.04	1.14AlaTrp: 1.14 ± 0.019	2.225AlaTyr: 2.225 ± 0.024	0.0AlaXaa: 0.0 ± 0.0
Cys	0.978CysAla: 0.978 ± 0.016	0.261CysCys: 0.261 ± 0.008	0.658CysAsp: 0.658 ± 0.012	0.596CysGlu: 0.596 ± 0.012	0.515CysPhe: 0.515 ± 0.012	0.986CysGly: 0.986 ± 0.018	0.315CysHis: 0.315 ± 0.008	0.712CysIle: 0.712 ± 0.014	0.524CysLys: 0.524 ± 0.01	1.17CysLeu: 1.17 ± 0.019	0.269CysMet: 0.269 ± 0.007	0.433CysAsn: 0.433 ± 0.011	0.62CysPro: 0.62 ± 0.015	0.428CysGln: 0.428 ± 0.01	0.692CysArg: 0.692 ± 0.014	0.888CysSer: 0.888 ± 0.016	0.711CysThr: 0.711 ± 0.014	0.776CysVal: 0.776 ± 0.013	0.2CysTrp: 0.2 ± 0.008	0.374CysTyr: 0.374 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	4.925AspAla: 4.925 ± 0.042	0.614AspCys: 0.614 ± 0.014	4.192AspAsp: 4.192 ± 0.047	4.54AspGlu: 4.54 ± 0.04	2.201AspPhe: 2.201 ± 0.023	4.274AspGly: 4.274 ± 0.035	1.109AspHis: 1.109 ± 0.015	3.192AspIle: 3.192 ± 0.027	2.466AspLys: 2.466 ± 0.029	4.85AspLeu: 4.85 ± 0.036	1.248AspMet: 1.248 ± 0.015	1.999AspAsn: 1.999 ± 0.025	3.045AspPro: 3.045 ± 0.026	1.716AspGln: 1.716 ± 0.018	2.799AspArg: 2.799 ± 0.023	4.144AspSer: 4.144 ± 0.038	3.004AspThr: 3.004 ± 0.024	3.682AspVal: 3.682 ± 0.026	0.829AspTrp: 0.829 ± 0.013	1.63AspTyr: 1.63 ± 0.02	0.0AspXaa: 0.0 ± 0.0
Glu	5.732GluAla: 5.732 ± 0.046	0.621GluCys: 0.621 ± 0.012	4.178GluAsp: 4.178 ± 0.043	5.772GluGlu: 5.772 ± 0.07	1.951GluPhe: 1.951 ± 0.023	4.28GluGly: 4.28 ± 0.037	1.287GluHis: 1.287 ± 0.018	3.145GluIle: 3.145 ± 0.029	3.829GluLys: 3.829 ± 0.038	5.201GluLeu: 5.201 ± 0.039	1.511GluMet: 1.511 ± 0.019	2.299GluAsn: 2.299 ± 0.028	2.706GluPro: 2.706 ± 0.032	2.303GluGln: 2.303 ± 0.028	3.931GluArg: 3.931 ± 0.04	4.343GluSer: 4.343 ± 0.036	3.431GluThr: 3.431 ± 0.029	3.831GluVal: 3.831 ± 0.034	0.874GluTrp: 0.874 ± 0.015	1.697GluTyr: 1.697 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	3.077PheAla: 3.077 ± 0.029	0.542PheCys: 0.542 ± 0.012	2.21PheAsp: 2.21 ± 0.025	2.158PheGlu: 2.158 ± 0.025	1.544PhePhe: 1.544 ± 0.025	2.939PheGly: 2.939 ± 0.032	0.823PheHis: 0.823 ± 0.014	1.869PheIle: 1.869 ± 0.022	1.593PheLys: 1.593 ± 0.018	3.252PheLeu: 3.252 ± 0.028	0.776PheMet: 0.776 ± 0.013	1.426PheAsn: 1.426 ± 0.018	1.877PhePro: 1.877 ± 0.024	1.345PheGln: 1.345 ± 0.019	1.793PheArg: 1.793 ± 0.018	2.891PheSer: 2.891 ± 0.027	2.16PheThr: 2.16 ± 0.023	2.339PheVal: 2.339 ± 0.028	0.62PheTrp: 0.62 ± 0.012	1.093PheTyr: 1.093 ± 0.019	0.0PheXaa: 0.0 ± 0.0
Gly	5.96GlyAla: 5.96 ± 0.046	0.891GlyCys: 0.891 ± 0.016	3.877GlyAsp: 3.877 ± 0.034	4.14GlyGlu: 4.14 ± 0.036	2.852GlyPhe: 2.852 ± 0.033	6.722GlyGly: 6.722 ± 0.069	1.635GlyHis: 1.635 ± 0.024	3.68GlyIle: 3.68 ± 0.029	3.83GlyLys: 3.83 ± 0.029	6.07GlyLeu: 6.07 ± 0.043	1.684GlyMet: 1.684 ± 0.022	2.716GlyAsn: 2.716 ± 0.03	3.344GlyPro: 3.344 ± 0.03	2.586GlyGln: 2.586 ± 0.028	4.173GlyArg: 4.173 ± 0.037	5.826GlySer: 5.826 ± 0.04	4.139GlyThr: 4.139 ± 0.035	4.629GlyVal: 4.629 ± 0.038	1.214GlyTrp: 1.214 ± 0.019	2.171GlyTyr: 2.171 ± 0.027	0.0GlyXaa: 0.0 ± 0.0
His	1.744HisAla: 1.744 ± 0.019	0.301HisCys: 0.301 ± 0.009	1.195HisAsp: 1.195 ± 0.018	1.244HisGlu: 1.244 ± 0.016	0.865HisPhe: 0.865 ± 0.013	1.645HisGly: 1.645 ± 0.021	0.751HisHis: 0.751 ± 0.016	1.219HisIle: 1.219 ± 0.015	0.937HisLys: 0.937 ± 0.014	2.105HisLeu: 2.105 ± 0.024	0.45HisMet: 0.45 ± 0.009	0.861HisAsn: 0.861 ± 0.013	1.533HisPro: 1.533 ± 0.02	0.909HisGln: 0.909 ± 0.015	1.361HisArg: 1.361 ± 0.021	1.736HisSer: 1.736 ± 0.022	1.274HisThr: 1.274 ± 0.017	1.334HisVal: 1.334 ± 0.018	0.305HisTrp: 0.305 ± 0.009	0.666HisTyr: 0.666 ± 0.011	0.0HisXaa: 0.0 ± 0.0
Ile	4.458IleAla: 4.458 ± 0.032	0.76IleCys: 0.76 ± 0.014	2.962IleAsp: 2.962 ± 0.027	2.968IleGlu: 2.968 ± 0.025	2.055IlePhe: 2.055 ± 0.024	3.388IleGly: 3.388 ± 0.031	1.135IleHis: 1.135 ± 0.019	2.724IleIle: 2.724 ± 0.032	2.387IleLys: 2.387 ± 0.026	4.573IleLeu: 4.573 ± 0.037	1.089IleMet: 1.089 ± 0.015	1.915IleAsn: 1.915 ± 0.022	3.07IlePro: 3.07 ± 0.028	1.874IleGln: 1.874 ± 0.021	2.703IleArg: 2.703 ± 0.025	4.054IleSer: 4.054 ± 0.033	3.061IleThr: 3.061 ± 0.028	3.236IleVal: 3.236 ± 0.032	0.717IleTrp: 0.717 ± 0.015	1.489IleTyr: 1.489 ± 0.02	0.0IleXaa: 0.0 ± 0.0
Lys	4.629LysAla: 4.629 ± 0.042	0.532LysCys: 0.532 ± 0.012	2.903LysAsp: 2.903 ± 0.031	3.642LysGlu: 3.642 ± 0.04	1.535LysPhe: 1.535 ± 0.017	3.3LysGly: 3.3 ± 0.028	1.091LysHis: 1.091 ± 0.017	2.409LysIle: 2.409 ± 0.025	3.501LysLys: 3.501 ± 0.051	4.242LysLeu: 4.242 ± 0.035	1.065LysMet: 1.065 ± 0.015	1.822LysAsn: 1.822 ± 0.025	2.78LysPro: 2.78 ± 0.031	1.796LysGln: 1.796 ± 0.02	3.401LysArg: 3.401 ± 0.029	3.769LysSer: 3.769 ± 0.035	2.915LysThr: 2.915 ± 0.028	3.043LysVal: 3.043 ± 0.033	0.697LysTrp: 0.697 ± 0.014	1.43LysTyr: 1.43 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	7.876LeuAla: 7.876 ± 0.046	1.134LeuCys: 1.134 ± 0.018	4.95LeuAsp: 4.95 ± 0.035	5.462LeuGlu: 5.462 ± 0.041	3.195LeuPhe: 3.195 ± 0.031	5.986LeuGly: 5.986 ± 0.041	2.093LeuHis: 2.093 ± 0.024	4.002LeuIle: 4.002 ± 0.03	4.348LeuLys: 4.348 ± 0.032	8.279LeuLeu: 8.279 ± 0.069	1.712LeuMet: 1.712 ± 0.021	3.131LeuAsn: 3.131 ± 0.026	5.38LeuPro: 5.38 ± 0.039	3.672LeuGln: 3.672 ± 0.042	5.386LeuArg: 5.386 ± 0.04	7.15LeuSer: 7.15 ± 0.048	4.787LeuThr: 4.787 ± 0.033	5.259LeuVal: 5.259 ± 0.046	1.166LeuTrp: 1.166 ± 0.016	2.317LeuTyr: 2.317 ± 0.023	0.0LeuXaa: 0.0 ± 0.0
Met	2.209MetAla: 2.209 ± 0.023	0.227MetCys: 0.227 ± 0.007	1.263MetAsp: 1.263 ± 0.018	1.323MetGlu: 1.323 ± 0.019	0.746MetPhe: 0.746 ± 0.013	1.57MetGly: 1.57 ± 0.022	0.481MetHis: 0.481 ± 0.011	0.982MetIle: 0.982 ± 0.017	1.063MetLys: 1.063 ± 0.017	1.805MetLeu: 1.805 ± 0.021	0.577MetMet: 0.577 ± 0.013	0.781MetAsn: 0.781 ± 0.014	1.239MetPro: 1.239 ± 0.018	0.834MetGln: 0.834 ± 0.017	1.253MetArg: 1.253 ± 0.017	1.834MetSer: 1.834 ± 0.021	1.23MetThr: 1.23 ± 0.017	1.348MetVal: 1.348 ± 0.016	0.258MetTrp: 0.258 ± 0.008	0.52MetTyr: 0.52 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.206AsnAla: 3.206 ± 0.028	0.464AsnCys: 0.464 ± 0.01	1.98AsnAsp: 1.98 ± 0.024	2.057AsnGlu: 2.057 ± 0.024	1.416AsnPhe: 1.416 ± 0.018	3.165AsnGly: 3.165 ± 0.029	0.835AsnHis: 0.835 ± 0.013	2.208AsnIle: 2.208 ± 0.021	1.622AsnLys: 1.622 ± 0.021	3.211AsnLeu: 3.211 ± 0.026	0.87AsnMet: 0.87 ± 0.016	1.583AsnAsn: 1.583 ± 0.022	2.396AsnPro: 2.396 ± 0.027	1.329AsnGln: 1.329 ± 0.017	1.902AsnArg: 1.902 ± 0.021	2.925AsnSer: 2.925 ± 0.027	2.289AsnThr: 2.289 ± 0.023	2.299AsnVal: 2.299 ± 0.02	0.559AsnTrp: 0.559 ± 0.01	1.115AsnTyr: 1.115 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	5.317ProAla: 5.317 ± 0.044	0.496ProCys: 0.496 ± 0.011	2.957ProAsp: 2.957 ± 0.026	3.653ProGlu: 3.653 ± 0.03	1.946ProPhe: 1.946 ± 0.02	3.88ProGly: 3.88 ± 0.036	1.271ProHis: 1.271 ± 0.015	2.629ProIle: 2.629 ± 0.027	2.824ProLys: 2.824 ± 0.033	4.723ProLeu: 4.723 ± 0.038	1.035ProMet: 1.035 ± 0.015	2.217ProAsn: 2.217 ± 0.025	4.91ProPro: 4.91 ± 0.072	2.397ProGln: 2.397 ± 0.031	3.3ProArg: 3.3 ± 0.034	5.75ProSer: 5.75 ± 0.048	4.206ProThr: 4.206 ± 0.042	3.514ProVal: 3.514 ± 0.034	0.704ProTrp: 0.704 ± 0.015	1.499ProTyr: 1.499 ± 0.022	0.0ProXaa: 0.0 ± 0.0
Gln	3.334GlnAla: 3.334 ± 0.033	0.438GlnCys: 0.438 ± 0.011	1.963GlnAsp: 1.963 ± 0.021	2.253GlnGlu: 2.253 ± 0.029	1.261GlnPhe: 1.261 ± 0.018	2.469GlnGly: 2.469 ± 0.027	0.971GlnHis: 0.971 ± 0.016	1.868GlnIle: 1.868 ± 0.022	1.96GlnLys: 1.96 ± 0.022	3.35GlnLeu: 3.35 ± 0.028	0.852GlnMet: 0.852 ± 0.014	1.549GlnAsn: 1.549 ± 0.02	2.397GlnPro: 2.397 ± 0.033	2.419GlnGln: 2.419 ± 0.059	2.462GlnArg: 2.462 ± 0.026	2.926GlnSer: 2.926 ± 0.029	2.239GlnThr: 2.239 ± 0.025	2.066GlnVal: 2.066 ± 0.024	0.55GlnTrp: 0.55 ± 0.011	1.182GlnTyr: 1.182 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	4.709ArgAla: 4.709 ± 0.036	0.704ArgCys: 0.704 ± 0.014	3.195ArgAsp: 3.195 ± 0.034	3.821ArgGlu: 3.821 ± 0.039	1.947ArgPhe: 1.947 ± 0.02	3.842ArgGly: 3.842 ± 0.039	1.464ArgHis: 1.464 ± 0.018	2.809ArgIle: 2.809 ± 0.025	3.339ArgLys: 3.339 ± 0.03	5.036ArgLeu: 5.036 ± 0.035	1.221ArgMet: 1.221 ± 0.017	2.203ArgAsn: 2.203 ± 0.022	3.268ArgPro: 3.268 ± 0.035	2.464ArgGln: 2.464 ± 0.026	4.773ArgArg: 4.773 ± 0.047	4.45ArgSer: 4.45 ± 0.036	3.156ArgThr: 3.156 ± 0.03	3.277ArgVal: 3.277 ± 0.029	0.821ArgTrp: 0.821 ± 0.015	1.521ArgTyr: 1.521 ± 0.018	0.0ArgXaa: 0.0 ± 0.0
Ser	6.776SerAla: 6.776 ± 0.043	0.879SerCys: 0.879 ± 0.015	4.217SerAsp: 4.217 ± 0.037	4.14SerGlu: 4.14 ± 0.034	2.95SerPhe: 2.95 ± 0.024	5.728SerGly: 5.728 ± 0.04	1.868SerHis: 1.868 ± 0.022	4.151SerIle: 4.151 ± 0.032	3.922SerLys: 3.922 ± 0.036	7.026SerLeu: 7.026 ± 0.045	1.639SerMet: 1.639 ± 0.021	3.124SerAsn: 3.124 ± 0.029	5.406SerPro: 5.406 ± 0.053	3.117SerGln: 3.117 ± 0.032	4.666SerArg: 4.666 ± 0.039	8.242SerSer: 8.242 ± 0.064	5.613SerThr: 5.613 ± 0.043	4.528SerVal: 4.528 ± 0.032	1.085SerTrp: 1.085 ± 0.016	2.113SerTyr: 2.113 ± 0.021	0.0SerXaa: 0.0 ± 0.0
Thr	5.437ThrAla: 5.437 ± 0.035	0.757ThrCys: 0.757 ± 0.013	2.92ThrAsp: 2.92 ± 0.025	3.208ThrGlu: 3.208 ± 0.029	2.206ThrPhe: 2.206 ± 0.022	4.313ThrGly: 4.313 ± 0.037	1.279ThrHis: 1.279 ± 0.017	3.246ThrIle: 3.246 ± 0.029	2.792ThrLys: 2.792 ± 0.026	5.232ThrLeu: 5.232 ± 0.039	1.248ThrMet: 1.248 ± 0.018	2.199ThrAsn: 2.199 ± 0.024	4.488ThrPro: 4.488 ± 0.042	2.059ThrGln: 2.059 ± 0.022	2.985ThrArg: 2.985 ± 0.027	5.365ThrSer: 5.365 ± 0.042	4.607ThrThr: 4.607 ± 0.051	3.725ThrVal: 3.725 ± 0.037	0.847ThrTrp: 0.847 ± 0.014	1.645ThrTyr: 1.645 ± 0.022	0.0ThrXaa: 0.0 ± 0.0
Val	5.67ValAla: 5.67 ± 0.042	0.785ValCys: 0.785 ± 0.013	3.814ValAsp: 3.814 ± 0.029	4.107ValGlu: 4.107 ± 0.039	2.339ValPhe: 2.339 ± 0.025	4.285ValGly: 4.285 ± 0.033	1.27ValHis: 1.27 ± 0.016	3.015ValIle: 3.015 ± 0.027	3.094ValLys: 3.094 ± 0.031	5.437ValLeu: 5.437 ± 0.039	1.32ValMet: 1.32 ± 0.018	2.254ValAsn: 2.254 ± 0.023	3.511ValPro: 3.511 ± 0.028	2.245ValGln: 2.245 ± 0.026	3.23ValArg: 3.23 ± 0.029	4.48ValSer: 4.48 ± 0.035	3.557ValThr: 3.557 ± 0.034	4.547ValVal: 4.547 ± 0.039	0.865ValTrp: 0.865 ± 0.015	1.696ValTyr: 1.696 ± 0.022	0.0ValXaa: 0.0 ± 0.0
Trp	1.183TrpAla: 1.183 ± 0.019	0.193TrpCys: 0.193 ± 0.007	0.915TrpAsp: 0.915 ± 0.016	0.838TrpGlu: 0.838 ± 0.014	0.514TrpPhe: 0.514 ± 0.012	0.981TrpGly: 0.981 ± 0.017	0.324TrpHis: 0.324 ± 0.008	0.73TrpIle: 0.73 ± 0.013	0.768TrpLys: 0.768 ± 0.014	1.248TrpLeu: 1.248 ± 0.016	0.352TrpMet: 0.352 ± 0.009	0.599TrpAsn: 0.599 ± 0.012	0.589TrpPro: 0.589 ± 0.012	0.524TrpGln: 0.524 ± 0.01	0.896TrpArg: 0.896 ± 0.014	1.013TrpSer: 1.013 ± 0.015	0.891TrpThr: 0.891 ± 0.014	0.908TrpVal: 0.908 ± 0.015	0.273TrpTrp: 0.273 ± 0.008	0.409TrpTyr: 0.409 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.191TyrAla: 2.191 ± 0.024	0.434TyrCys: 0.434 ± 0.009	1.667TyrAsp: 1.667 ± 0.019	1.553TyrGlu: 1.553 ± 0.021	1.183TyrPhe: 1.183 ± 0.016	2.136TyrGly: 2.136 ± 0.026	0.706TyrHis: 0.706 ± 0.013	1.477TyrIle: 1.477 ± 0.02	1.199TyrLys: 1.199 ± 0.017	2.585TyrLeu: 2.585 ± 0.025	0.599TyrMet: 0.599 ± 0.011	1.213TyrAsn: 1.213 ± 0.017	1.466TyrPro: 1.466 ± 0.02	1.076TyrGln: 1.076 ± 0.017	1.498TyrArg: 1.498 ± 0.022	2.115TyrSer: 2.115 ± 0.022	1.673TyrThr: 1.673 ± 0.022	1.599TyrVal: 1.599 ± 0.02	0.422TyrTrp: 0.422 ± 0.009	0.938TyrTyr: 0.938 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9928 proteins (4665772 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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