Phyllobacterium sp. OV277

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Phyllobacterium; unclassified Phyllobacterium

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5202 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H0GY20|A0A1H0GY20_9RHIZ TYR_PHOSPHATASE_2 domain-containing protein OS=Phyllobacterium sp. OV277 OX=1882772 GN=SAMN05443582_1011016 PE=4 SV=1
MM1 pKa = 7.65PSYY4 pKa = 10.43SARR7 pKa = 11.84YY8 pKa = 6.8DD9 pKa = 3.29TRR11 pKa = 11.84EE12 pKa = 3.9ALMEE16 pKa = 4.4ALVEE20 pKa = 4.89LMALDD25 pKa = 3.69IPVEE29 pKa = 3.98EE30 pKa = 4.67FAPVLCTIGPVDD42 pKa = 5.18LDD44 pKa = 4.02LLADD48 pKa = 5.28CIHH51 pKa = 7.37DD52 pKa = 4.25AGINDD57 pKa = 3.76TRR59 pKa = 11.84SIAAA63 pKa = 3.98

Molecular weight: 6.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H0TQS5|A0A1H0TQS5_9RHIZ Uncharacterized conserved protein DUF952 family OS=Phyllobacterium sp. OV277 OX=1882772 GN=SAMN05443582_10615 PE=4 SV=1
MM1 pKa = 7.02NAGMKK6 pKa = 10.42LLLRR10 pKa = 11.84QKK12 pKa = 10.29KK13 pKa = 9.62AVVGLTIVVALCLMALFAPILAPNDD38 pKa = 3.11PAARR42 pKa = 11.84VGRR45 pKa = 11.84SHH47 pKa = 6.2QPPSIEE53 pKa = 3.94HH54 pKa = 5.76VMGTTKK60 pKa = 10.01MGRR63 pKa = 11.84DD64 pKa = 3.53VFSQFVWGARR74 pKa = 11.84SSLTIGFATGIFITVLGTLIGLAGGYY100 pKa = 10.06FGGRR104 pKa = 11.84TDD106 pKa = 5.21NILDD110 pKa = 4.16LLTNAVLVIPNIPLLILLASFAGTVGPVAIMAIIALTSWPWGARR154 pKa = 11.84MTRR157 pKa = 11.84SQTLALRR164 pKa = 11.84NRR166 pKa = 11.84EE167 pKa = 4.02FVVAARR173 pKa = 11.84MVGEE177 pKa = 4.16PNWRR181 pKa = 11.84IVFVEE186 pKa = 4.25IVPNLLPLIALNLVGSIIYY205 pKa = 10.22AIVAQTTLEE214 pKa = 3.95YY215 pKa = 10.9LGFGDD220 pKa = 4.61PLNVTWGTMLYY231 pKa = 10.16NAQNSSAIIVGAWWDD246 pKa = 3.44IAVPCAGIVLVGLGLSLLNFTFDD269 pKa = 4.74EE270 pKa = 4.32IANPQLRR277 pKa = 11.84SGPALRR283 pKa = 11.84RR284 pKa = 11.84WLRR287 pKa = 11.84LNRR290 pKa = 11.84LRR292 pKa = 11.84TRR294 pKa = 11.84EE295 pKa = 4.0LEE297 pKa = 3.99AARR300 pKa = 4.4

Molecular weight: 32.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5202

0

5202

1587530

27

3579

305.2

33.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.202 ± 0.044

0.752 ± 0.008

5.615 ± 0.025

5.45 ± 0.033

4.022 ± 0.023

8.196 ± 0.029

2.034 ± 0.017

6.173 ± 0.028

4.152 ± 0.027

9.947 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.018

3.184 ± 0.022

4.824 ± 0.023

3.251 ± 0.017

6.113 ± 0.032

5.906 ± 0.027

5.642 ± 0.035

7.296 ± 0.028

1.278 ± 0.014

2.432 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski