Amino acid dipepetide frequency for Phyllobacterium sp. OV277

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.644AlaAla: 13.644 ± 0.11
0.802AlaCys: 0.802 ± 0.022
6.12AlaAsp: 6.12 ± 0.062
6.396AlaGlu: 6.396 ± 0.07
4.473AlaPhe: 4.473 ± 0.055
9.481AlaGly: 9.481 ± 0.094
2.035AlaHis: 2.035 ± 0.042
7.075AlaIle: 7.075 ± 0.085
4.94AlaLys: 4.94 ± 0.066
11.892AlaLeu: 11.892 ± 0.092
3.209AlaMet: 3.209 ± 0.043
3.286AlaAsn: 3.286 ± 0.047
4.501AlaPro: 4.501 ± 0.065
3.617AlaGln: 3.617 ± 0.051
6.45AlaArg: 6.45 ± 0.073
6.337AlaSer: 6.337 ± 0.064
5.863AlaThr: 5.863 ± 0.079
8.034AlaVal: 8.034 ± 0.077
1.237AlaTrp: 1.237 ± 0.029
2.631AlaTyr: 2.631 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.776CysAla: 0.776 ± 0.022
0.094CysCys: 0.094 ± 0.009
0.445CysAsp: 0.445 ± 0.019
0.382CysGlu: 0.382 ± 0.017
0.346CysPhe: 0.346 ± 0.016
0.804CysGly: 0.804 ± 0.022
0.212CysHis: 0.212 ± 0.013
0.408CysIle: 0.408 ± 0.016
0.216CysLys: 0.216 ± 0.014
0.706CysLeu: 0.706 ± 0.02
0.15CysMet: 0.15 ± 0.01
0.232CysAsn: 0.232 ± 0.012
0.368CysPro: 0.368 ± 0.017
0.205CysGln: 0.205 ± 0.013
0.464CysArg: 0.464 ± 0.018
0.455CysSer: 0.455 ± 0.019
0.432CysThr: 0.432 ± 0.021
0.541CysVal: 0.541 ± 0.018
0.1CysTrp: 0.1 ± 0.01
0.183CysTyr: 0.183 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
6.118AspAla: 6.118 ± 0.068
0.429AspCys: 0.429 ± 0.018
3.09AspAsp: 3.09 ± 0.05
3.398AspGlu: 3.398 ± 0.051
2.26AspPhe: 2.26 ± 0.04
4.751AspGly: 4.751 ± 0.064
1.28AspHis: 1.28 ± 0.031
3.669AspIle: 3.669 ± 0.058
2.285AspLys: 2.285 ± 0.04
5.438AspLeu: 5.438 ± 0.061
1.419AspMet: 1.419 ± 0.028
1.693AspAsn: 1.693 ± 0.03
3.091AspPro: 3.091 ± 0.052
1.849AspGln: 1.849 ± 0.037
3.75AspArg: 3.75 ± 0.057
2.279AspSer: 2.279 ± 0.046
2.722AspThr: 2.722 ± 0.047
4.168AspVal: 4.168 ± 0.048
0.875AspTrp: 0.875 ± 0.022
1.582AspTyr: 1.582 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
6.483GluAla: 6.483 ± 0.073
0.321GluCys: 0.321 ± 0.015
2.573GluAsp: 2.573 ± 0.044
3.051GluGlu: 3.051 ± 0.057
1.85GluPhe: 1.85 ± 0.033
3.791GluGly: 3.791 ± 0.053
1.211GluHis: 1.211 ± 0.03
3.689GluIle: 3.689 ± 0.051
2.9GluLys: 2.9 ± 0.047
5.09GluLeu: 5.09 ± 0.064
1.461GluMet: 1.461 ± 0.032
1.949GluAsn: 1.949 ± 0.034
2.619GluPro: 2.619 ± 0.047
2.159GluGln: 2.159 ± 0.043
4.164GluArg: 4.164 ± 0.051
2.409GluSer: 2.409 ± 0.043
3.576GluThr: 3.576 ± 0.045
3.372GluVal: 3.372 ± 0.05
0.704GluTrp: 0.704 ± 0.023
1.129GluTyr: 1.129 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
4.578PheAla: 4.578 ± 0.059
0.349PheCys: 0.349 ± 0.014
2.759PheAsp: 2.759 ± 0.046
2.207PheGlu: 2.207 ± 0.041
1.664PhePhe: 1.664 ± 0.038
3.795PheGly: 3.795 ± 0.055
0.843PheHis: 0.843 ± 0.023
2.271PheIle: 2.271 ± 0.041
1.335PheLys: 1.335 ± 0.033
3.704PheLeu: 3.704 ± 0.055
0.919PheMet: 0.919 ± 0.026
1.347PheAsn: 1.347 ± 0.033
1.698PhePro: 1.698 ± 0.033
1.184PheGln: 1.184 ± 0.031
2.161PheArg: 2.161 ± 0.035
2.576PheSer: 2.576 ± 0.053
2.183PheThr: 2.183 ± 0.039
3.003PheVal: 3.003 ± 0.045
0.574PheTrp: 0.574 ± 0.023
1.068PheTyr: 1.068 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
7.911GlyAla: 7.911 ± 0.081
0.745GlyCys: 0.745 ± 0.025
4.127GlyAsp: 4.127 ± 0.057
4.371GlyGlu: 4.371 ± 0.053
3.819GlyPhe: 3.819 ± 0.051
6.632GlyGly: 6.632 ± 0.083
1.786GlyHis: 1.786 ± 0.036
5.336GlyIle: 5.336 ± 0.066
4.023GlyLys: 4.023 ± 0.05
8.453GlyLeu: 8.453 ± 0.088
2.213GlyMet: 2.213 ± 0.04
2.667GlyAsn: 2.667 ± 0.058
3.061GlyPro: 3.061 ± 0.054
2.796GlyGln: 2.796 ± 0.046
4.84GlyArg: 4.84 ± 0.051
4.968GlySer: 4.968 ± 0.067
4.783GlyThr: 4.783 ± 0.074
5.795GlyVal: 5.795 ± 0.067
1.256GlyTrp: 1.256 ± 0.033
2.375GlyTyr: 2.375 ± 0.039
0.0GlyXaa: 0.0 ± 0.0
His
2.07HisAla: 2.07 ± 0.046
0.206HisCys: 0.206 ± 0.011
1.22HisAsp: 1.22 ± 0.027
1.09HisGlu: 1.09 ± 0.028
0.928HisPhe: 0.928 ± 0.026
1.814HisGly: 1.814 ± 0.039
0.558HisHis: 0.558 ± 0.02
1.146HisIle: 1.146 ± 0.028
0.627HisLys: 0.627 ± 0.02
2.04HisLeu: 2.04 ± 0.036
0.543HisMet: 0.543 ± 0.018
0.557HisAsn: 0.557 ± 0.018
1.298HisPro: 1.298 ± 0.028
0.645HisGln: 0.645 ± 0.021
1.232HisArg: 1.232 ± 0.026
1.061HisSer: 1.061 ± 0.026
0.897HisThr: 0.897 ± 0.025
1.457HisVal: 1.457 ± 0.033
0.326HisTrp: 0.326 ± 0.015
0.627HisTyr: 0.627 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
8.202IleAla: 8.202 ± 0.072
0.591IleCys: 0.591 ± 0.02
4.035IleAsp: 4.035 ± 0.055
3.713IleGlu: 3.713 ± 0.05
2.228IlePhe: 2.228 ± 0.041
5.548IleGly: 5.548 ± 0.071
1.087IleHis: 1.087 ± 0.03
3.575IleIle: 3.575 ± 0.054
2.14IleLys: 2.14 ± 0.042
5.545IleLeu: 5.545 ± 0.066
1.227IleMet: 1.227 ± 0.028
1.987IleAsn: 1.987 ± 0.041
2.69IlePro: 2.69 ± 0.042
1.482IleGln: 1.482 ± 0.036
3.481IleArg: 3.481 ± 0.046
3.921IleSer: 3.921 ± 0.056
3.287IleThr: 3.287 ± 0.058
4.944IleVal: 4.944 ± 0.06
0.695IleTrp: 0.695 ± 0.02
1.354IleTyr: 1.354 ± 0.033
0.0IleXaa: 0.0 ± 0.0
Lys
4.896LysAla: 4.896 ± 0.072
0.171LysCys: 0.171 ± 0.009
2.304LysAsp: 2.304 ± 0.045
2.008LysGlu: 2.008 ± 0.041
1.243LysPhe: 1.243 ± 0.029
3.163LysGly: 3.163 ± 0.045
0.737LysHis: 0.737 ± 0.022
2.441LysIle: 2.441 ± 0.039
1.891LysLys: 1.891 ± 0.042
4.162LysLeu: 4.162 ± 0.053
1.01LysMet: 1.01 ± 0.028
1.386LysAsn: 1.386 ± 0.033
2.606LysPro: 2.606 ± 0.047
1.419LysGln: 1.419 ± 0.032
2.75LysArg: 2.75 ± 0.045
2.565LysSer: 2.565 ± 0.037
2.661LysThr: 2.661 ± 0.045
2.813LysVal: 2.813 ± 0.044
0.474LysTrp: 0.474 ± 0.019
0.824LysTyr: 0.824 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
11.894LeuAla: 11.894 ± 0.107
0.835LeuCys: 0.835 ± 0.025
5.764LeuAsp: 5.764 ± 0.062
5.034LeuGlu: 5.034 ± 0.062
3.871LeuPhe: 3.871 ± 0.067
8.029LeuGly: 8.029 ± 0.083
1.783LeuHis: 1.783 ± 0.04
6.019LeuIle: 6.019 ± 0.078
4.375LeuLys: 4.375 ± 0.059
9.308LeuLeu: 9.308 ± 0.094
2.351LeuMet: 2.351 ± 0.037
3.065LeuAsn: 3.065 ± 0.044
5.105LeuPro: 5.105 ± 0.063
3.013LeuGln: 3.013 ± 0.046
5.797LeuArg: 5.797 ± 0.069
6.817LeuSer: 6.817 ± 0.063
5.756LeuThr: 5.756 ± 0.061
7.308LeuVal: 7.308 ± 0.084
1.091LeuTrp: 1.091 ± 0.032
2.256LeuTyr: 2.256 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
2.912MetAla: 2.912 ± 0.04
0.14MetCys: 0.14 ± 0.011
1.133MetAsp: 1.133 ± 0.031
1.077MetGlu: 1.077 ± 0.025
0.749MetPhe: 0.749 ± 0.022
1.786MetGly: 1.786 ± 0.032
0.475MetHis: 0.475 ± 0.017
1.631MetIle: 1.631 ± 0.031
1.119MetLys: 1.119 ± 0.029
2.493MetLeu: 2.493 ± 0.043
0.699MetMet: 0.699 ± 0.021
0.911MetAsn: 0.911 ± 0.022
1.386MetPro: 1.386 ± 0.031
0.963MetGln: 0.963 ± 0.025
1.807MetArg: 1.807 ± 0.036
1.772MetSer: 1.772 ± 0.032
2.003MetThr: 2.003 ± 0.039
1.71MetVal: 1.71 ± 0.033
0.204MetTrp: 0.204 ± 0.011
0.331MetTyr: 0.331 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.55AsnAla: 3.55 ± 0.048
0.248AsnCys: 0.248 ± 0.014
1.758AsnAsp: 1.758 ± 0.043
1.57AsnGlu: 1.57 ± 0.033
1.184AsnPhe: 1.184 ± 0.028
2.819AsnGly: 2.819 ± 0.047
0.647AsnHis: 0.647 ± 0.02
1.92AsnIle: 1.92 ± 0.035
1.046AsnLys: 1.046 ± 0.029
3.041AsnLeu: 3.041 ± 0.044
0.758AsnMet: 0.758 ± 0.022
1.046AsnAsn: 1.046 ± 0.029
2.107AsnPro: 2.107 ± 0.041
0.954AsnGln: 0.954 ± 0.028
2.042AsnArg: 2.042 ± 0.034
1.71AsnSer: 1.71 ± 0.034
1.694AsnThr: 1.694 ± 0.047
2.365AsnVal: 2.365 ± 0.043
0.523AsnTrp: 0.523 ± 0.019
0.855AsnTyr: 0.855 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
5.236ProAla: 5.236 ± 0.072
0.29ProCys: 0.29 ± 0.014
3.522ProAsp: 3.522 ± 0.054
3.432ProGlu: 3.432 ± 0.053
2.039ProPhe: 2.039 ± 0.036
3.744ProGly: 3.744 ± 0.054
1.051ProHis: 1.051 ± 0.022
2.567ProIle: 2.567 ± 0.043
1.99ProLys: 1.99 ± 0.031
4.619ProLeu: 4.619 ± 0.057
1.068ProMet: 1.068 ± 0.024
1.453ProAsn: 1.453 ± 0.031
1.811ProPro: 1.811 ± 0.04
1.686ProGln: 1.686 ± 0.037
2.328ProArg: 2.328 ± 0.048
2.818ProSer: 2.818 ± 0.048
2.398ProThr: 2.398 ± 0.047
4.339ProVal: 4.339 ± 0.059
0.603ProTrp: 0.603 ± 0.021
1.241ProTyr: 1.241 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
3.727GlnAla: 3.727 ± 0.048
0.18GlnCys: 0.18 ± 0.009
1.424GlnAsp: 1.424 ± 0.032
1.503GlnGlu: 1.503 ± 0.027
1.269GlnPhe: 1.269 ± 0.025
2.254GlnGly: 2.254 ± 0.043
0.648GlnHis: 0.648 ± 0.018
2.159GlnIle: 2.159 ± 0.036
1.456GlnLys: 1.456 ± 0.032
3.101GlnLeu: 3.101 ± 0.047
0.934GlnMet: 0.934 ± 0.023
1.112GlnAsn: 1.112 ± 0.028
1.702GlnPro: 1.702 ± 0.032
1.322GlnGln: 1.322 ± 0.034
2.333GlnArg: 2.333 ± 0.04
2.144GlnSer: 2.144 ± 0.04
2.02GlnThr: 2.02 ± 0.037
2.052GlnVal: 2.052 ± 0.034
0.415GlnTrp: 0.415 ± 0.014
0.756GlnTyr: 0.756 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
6.055ArgAla: 6.055 ± 0.068
0.391ArgCys: 0.391 ± 0.018
3.489ArgAsp: 3.489 ± 0.049
3.508ArgGlu: 3.508 ± 0.056
2.809ArgPhe: 2.809 ± 0.044
3.914ArgGly: 3.914 ± 0.05
1.475ArgHis: 1.475 ± 0.034
4.05ArgIle: 4.05 ± 0.055
2.562ArgLys: 2.562 ± 0.043
6.548ArgLeu: 6.548 ± 0.071
1.652ArgMet: 1.652 ± 0.03
2.174ArgAsn: 2.174 ± 0.033
2.756ArgPro: 2.756 ± 0.047
2.335ArgGln: 2.335 ± 0.042
4.153ArgArg: 4.153 ± 0.064
3.581ArgSer: 3.581 ± 0.058
3.133ArgThr: 3.133 ± 0.045
4.108ArgVal: 4.108 ± 0.061
0.803ArgTrp: 0.803 ± 0.023
1.635ArgTyr: 1.635 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
6.189SerAla: 6.189 ± 0.058
0.38SerCys: 0.38 ± 0.016
3.233SerAsp: 3.233 ± 0.044
2.931SerGlu: 2.931 ± 0.051
2.586SerPhe: 2.586 ± 0.041
5.933SerGly: 5.933 ± 0.076
1.223SerHis: 1.223 ± 0.03
3.585SerIle: 3.585 ± 0.047
2.254SerLys: 2.254 ± 0.038
5.914SerLeu: 5.914 ± 0.071
1.561SerMet: 1.561 ± 0.03
1.793SerAsn: 1.793 ± 0.037
2.811SerPro: 2.811 ± 0.045
1.8SerGln: 1.8 ± 0.034
3.448SerArg: 3.448 ± 0.051
3.51SerSer: 3.51 ± 0.052
3.188SerThr: 3.188 ± 0.046
4.407SerVal: 4.407 ± 0.05
0.809SerTrp: 0.809 ± 0.024
1.508SerTyr: 1.508 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
6.087ThrAla: 6.087 ± 0.081
0.41ThrCys: 0.41 ± 0.02
2.979ThrAsp: 2.979 ± 0.047
2.735ThrGlu: 2.735 ± 0.038
2.212ThrPhe: 2.212 ± 0.042
5.247ThrGly: 5.247 ± 0.068
1.104ThrHis: 1.104 ± 0.029
3.75ThrIle: 3.75 ± 0.055
2.034ThrLys: 2.034 ± 0.036
5.997ThrLeu: 5.997 ± 0.067
1.32ThrMet: 1.32 ± 0.026
1.664ThrAsn: 1.664 ± 0.035
3.277ThrPro: 3.277 ± 0.06
1.597ThrGln: 1.597 ± 0.038
3.11ThrArg: 3.11 ± 0.047
3.276ThrSer: 3.276 ± 0.053
3.174ThrThr: 3.174 ± 0.055
4.498ThrVal: 4.498 ± 0.058
0.615ThrTrp: 0.615 ± 0.02
1.337ThrTyr: 1.337 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
8.067ValAla: 8.067 ± 0.083
0.586ValCys: 0.586 ± 0.021
4.023ValAsp: 4.023 ± 0.053
4.237ValGlu: 4.237 ± 0.054
3.063ValPhe: 3.063 ± 0.049
5.34ValGly: 5.34 ± 0.067
1.384ValHis: 1.384 ± 0.033
4.511ValIle: 4.511 ± 0.062
2.956ValLys: 2.956 ± 0.041
7.391ValLeu: 7.391 ± 0.086
1.929ValMet: 1.929 ± 0.038
2.253ValAsn: 2.253 ± 0.044
3.568ValPro: 3.568 ± 0.049
2.097ValGln: 2.097 ± 0.032
4.228ValArg: 4.228 ± 0.053
4.664ValSer: 4.664 ± 0.055
4.607ValThr: 4.607 ± 0.062
5.582ValVal: 5.582 ± 0.081
0.865ValTrp: 0.865 ± 0.023
1.611ValTyr: 1.611 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.113TrpAla: 1.113 ± 0.029
0.11TrpCys: 0.11 ± 0.009
0.623TrpAsp: 0.623 ± 0.02
0.501TrpGlu: 0.501 ± 0.018
0.573TrpPhe: 0.573 ± 0.017
0.869TrpGly: 0.869 ± 0.025
0.294TrpHis: 0.294 ± 0.015
0.722TrpIle: 0.722 ± 0.024
0.542TrpLys: 0.542 ± 0.024
1.573TrpLeu: 1.573 ± 0.034
0.375TrpMet: 0.375 ± 0.016
0.515TrpAsn: 0.515 ± 0.02
0.6TrpPro: 0.6 ± 0.022
0.58TrpGln: 0.58 ± 0.019
0.917TrpArg: 0.917 ± 0.023
0.813TrpSer: 0.813 ± 0.023
0.721TrpThr: 0.721 ± 0.025
0.788TrpVal: 0.788 ± 0.023
0.217TrpTrp: 0.217 ± 0.012
0.334TrpTyr: 0.334 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.512TyrAla: 2.512 ± 0.044
0.242TyrCys: 0.242 ± 0.011
1.528TyrAsp: 1.528 ± 0.033
1.357TyrGlu: 1.357 ± 0.029
1.051TyrPhe: 1.051 ± 0.026
2.239TyrGly: 2.239 ± 0.037
0.511TyrHis: 0.511 ± 0.016
1.21TyrIle: 1.21 ± 0.028
0.832TyrLys: 0.832 ± 0.024
2.454TyrLeu: 2.454 ± 0.047
0.503TyrMet: 0.503 ± 0.017
0.748TyrAsn: 0.748 ± 0.026
1.183TyrPro: 1.183 ± 0.027
0.807TyrGln: 0.807 ± 0.026
1.678TyrArg: 1.678 ± 0.038
1.389TyrSer: 1.389 ± 0.029
1.323TyrThr: 1.323 ± 0.037
1.676TyrVal: 1.676 ± 0.038
0.393TyrTrp: 0.393 ± 0.018
0.682TyrTyr: 0.682 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5202 proteins (1587530 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski