Hubei polero-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KF08|A0A1L3KF08_9VIRU Serine protease OS=Hubei polero-like virus 1 OX=1923169 PE=4 SV=1
MM1 pKa = 7.27SVSLVDD7 pKa = 3.44QLVARR12 pKa = 11.84VLFQNQNKK20 pKa = 9.66RR21 pKa = 11.84EE22 pKa = 3.94IALWRR27 pKa = 11.84ALPSKK32 pKa = 10.37PGFGLSTDD40 pKa = 3.67GQVLDD45 pKa = 5.29FVQCLARR52 pKa = 11.84QINVPSEE59 pKa = 3.96EE60 pKa = 4.05VVEE63 pKa = 3.93NWEE66 pKa = 4.35RR67 pKa = 11.84YY68 pKa = 8.75LVPTDD73 pKa = 3.61CSGFDD78 pKa = 3.21WSVAEE83 pKa = 5.47WMLQDD88 pKa = 4.67DD89 pKa = 4.11MEE91 pKa = 4.38VRR93 pKa = 11.84NRR95 pKa = 11.84LTTDD99 pKa = 4.09LNPLTRR105 pKa = 11.84RR106 pKa = 11.84LRR108 pKa = 11.84AGWLKK113 pKa = 10.66CLSNSVLSLSDD124 pKa = 3.28GTLLAQRR131 pKa = 11.84VPGVQKK137 pKa = 10.45SGSYY141 pKa = 8.27NTSSTNSRR149 pKa = 11.84IRR151 pKa = 11.84VMAAYY156 pKa = 9.95HH157 pKa = 6.63CGADD161 pKa = 3.2WAIAMGDD168 pKa = 3.79DD169 pKa = 3.96ALEE172 pKa = 4.34SANSNLEE179 pKa = 4.08EE180 pKa = 4.12YY181 pKa = 10.45KK182 pKa = 10.88SLGFKK187 pKa = 10.75VEE189 pKa = 3.93VSGQLEE195 pKa = 4.67FCSHH199 pKa = 5.47TFVRR203 pKa = 11.84PDD205 pKa = 3.0LALPVNIGKK214 pKa = 8.35MLYY217 pKa = 10.02KK218 pKa = 10.68LIYY221 pKa = 9.82GYY223 pKa = 10.31EE224 pKa = 4.1AGSGSLEE231 pKa = 4.17VVTNYY236 pKa = 10.7LNACFAVLNEE246 pKa = 4.34LRR248 pKa = 11.84HH249 pKa = 5.99DD250 pKa = 4.15PEE252 pKa = 4.61TVRR255 pKa = 11.84LLYY258 pKa = 9.99QWLVLPVQPQNEE270 pKa = 4.13

Molecular weight: 30.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KF55|A0A1L3KF55_9VIRU Uncharacterized protein OS=Hubei polero-like virus 1 OX=1923169 PE=4 SV=1
MM1 pKa = 6.52NTVVVRR7 pKa = 11.84RR8 pKa = 11.84NGRR11 pKa = 11.84RR12 pKa = 11.84GLRR15 pKa = 11.84LRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84NARR25 pKa = 11.84TQQMVVVTQPQRR37 pKa = 11.84TQRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84QRR45 pKa = 11.84RR46 pKa = 11.84GRR48 pKa = 11.84GTSSRR53 pKa = 11.84RR54 pKa = 11.84GAVGRR59 pKa = 11.84GNNFEE64 pKa = 4.28TFVFSKK70 pKa = 11.1DD71 pKa = 3.43NLKK74 pKa = 10.86GSSKK78 pKa = 9.92GTFTFGPSLSDD89 pKa = 3.45CPAFASGILKK99 pKa = 10.22AYY101 pKa = 9.97HH102 pKa = 6.43EE103 pKa = 4.51YY104 pKa = 10.82KK105 pKa = 10.39IISCNMEE112 pKa = 4.67FISEE116 pKa = 4.23ASSTAAGSIAYY127 pKa = 9.34EE128 pKa = 4.13LDD130 pKa = 3.49PHH132 pKa = 7.36CKK134 pKa = 9.33LTDD137 pKa = 3.64LASTVNKK144 pKa = 10.02FSITKK149 pKa = 9.78GGKK152 pKa = 8.06KK153 pKa = 8.45TFTARR158 pKa = 11.84LINGVEE164 pKa = 3.8WHH166 pKa = 7.3DD167 pKa = 3.52ATEE170 pKa = 4.15DD171 pKa = 3.45QFRR174 pKa = 11.84VHH176 pKa = 6.3YY177 pKa = 9.76KK178 pKa = 11.03GNGDD182 pKa = 3.54STTAGSFRR190 pKa = 11.84ITIRR194 pKa = 11.84VATQNPKK201 pKa = 10.5

Molecular weight: 22.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1101

201

630

367.0

40.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.811 ± 0.59

1.544 ± 0.254

3.361 ± 0.712

5.268 ± 0.587

4.178 ± 0.515

7.357 ± 0.619

1.817 ± 0.256

3.724 ± 0.544

5.086 ± 0.766

9.628 ± 1.808

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.635 ± 0.214

4.632 ± 0.641

5.54 ± 1.299

3.815 ± 0.403

6.358 ± 2.27

9.446 ± 0.554

6.812 ± 1.115

7.175 ± 0.889

1.998 ± 0.468

2.816 ± 0.277
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski