Streptococcus ictaluri 707-05
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2397 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G5K0G1|G5K0G1_9STRE DNA-binding helix-turn-helix protein OS=Streptococcus ictaluri 707-05 OX=764299 GN=STRIC_0083 PE=4 SV=1
MM1 pKa = 7.62 ALAKK5 pKa = 9.78 IVYY8 pKa = 10.47 ASMTGNTEE16 pKa = 3.89 EE17 pKa = 4.5 IADD20 pKa = 3.67 IVASKK25 pKa = 10.31 LQEE28 pKa = 3.8 LGYY31 pKa = 10.61 DD32 pKa = 3.69 VEE34 pKa = 4.76 IDD36 pKa = 3.2 EE37 pKa = 4.77 CTSVEE42 pKa = 3.77 ASEE45 pKa = 5.91 FEE47 pKa = 4.29 DD48 pKa = 4.24 ADD50 pKa = 3.73 MAIVATYY57 pKa = 9.3 TYY59 pKa = 11.24 GDD61 pKa = 3.61 GDD63 pKa = 4.31 LPDD66 pKa = 5.1 EE67 pKa = 4.45 IVDD70 pKa = 4.62 FYY72 pKa = 11.83 EE73 pKa = 5.1 DD74 pKa = 4.37 LQDD77 pKa = 4.62 LDD79 pKa = 4.65 LSGKK83 pKa = 9.71 IYY85 pKa = 10.62 GVVGSGDD92 pKa = 3.05 TFYY95 pKa = 11.14 DD96 pKa = 3.99 YY97 pKa = 11.04 FCKK100 pKa = 10.6 SVDD103 pKa = 3.35 EE104 pKa = 4.79 FEE106 pKa = 4.48 EE107 pKa = 4.38 QFALTGATKK116 pKa = 10.51 GADD119 pKa = 3.37 SVKK122 pKa = 10.32 VDD124 pKa = 4.66 LAAEE128 pKa = 4.42 DD129 pKa = 4.26 EE130 pKa = 4.87 DD131 pKa = 4.27 IKK133 pKa = 11.2 HH134 pKa = 6.42 LEE136 pKa = 3.95 AFAEE140 pKa = 4.31 KK141 pKa = 9.84 MSQALEE147 pKa = 4.44 TII149 pKa = 4.05
Molecular weight: 16.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.617
IPC_protein 3.605
Toseland 3.401
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.516
Rodwell 3.439
Grimsley 3.312
Solomon 3.579
Lehninger 3.541
Nozaki 3.719
DTASelect 3.91
Thurlkill 3.452
EMBOSS 3.528
Sillero 3.732
Patrickios 0.985
IPC_peptide 3.579
IPC2_peptide 3.706
IPC2.peptide.svr19 3.689
Protein with the highest isoelectric point:
>tr|G5K4X0|G5K4X0_9STRE Ribonuclease M5 OS=Streptococcus ictaluri 707-05 OX=764299 GN=rnmV PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.67 IRR11 pKa = 11.84 RR12 pKa = 11.84 QRR14 pKa = 11.84 KK15 pKa = 7.52 HH16 pKa = 5.2 GFRR19 pKa = 11.84 HH20 pKa = 6.39 RR21 pKa = 11.84 MSTKK25 pKa = 9.02 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.81 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLSAA44 pKa = 4.05
Molecular weight: 5.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2397
0
2397
639083
37
1465
266.6
29.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.581 ± 0.065
0.6 ± 0.015
5.693 ± 0.044
6.591 ± 0.054
4.5 ± 0.04
6.291 ± 0.047
2.034 ± 0.021
7.274 ± 0.048
7.158 ± 0.051
10.474 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.62 ± 0.027
4.331 ± 0.036
3.319 ± 0.03
4.361 ± 0.036
3.968 ± 0.037
6.553 ± 0.041
5.536 ± 0.04
6.497 ± 0.042
0.901 ± 0.018
3.718 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here