Sunshine Coast virus
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3VIZ0|I3VIZ0_9MONO Matrix OS=Sunshine Coast virus OX=1195087 PE=4 SV=1
MM1 pKa = 7.41 EE2 pKa = 5.31 LAGSVLDD9 pKa = 4.5 VKK11 pKa = 9.95 TQTLMEE17 pKa = 5.29 GILAATKK24 pKa = 9.93 EE25 pKa = 4.24 DD26 pKa = 3.92 TVISDD31 pKa = 3.6 WSSDD35 pKa = 2.37 IDD37 pKa = 3.44 TAMKK41 pKa = 10.65 NNSDD45 pKa = 3.66 PQIPAWASGVNYY57 pKa = 10.56 NIDD60 pKa = 3.39 EE61 pKa = 4.77 AEE63 pKa = 4.35 DD64 pKa = 3.37 EE65 pKa = 4.68 KK66 pKa = 11.36 QALDD70 pKa = 3.76 CKK72 pKa = 10.51 DD73 pKa = 3.28 ISVEE77 pKa = 4.01 EE78 pKa = 4.36 YY79 pKa = 10.98 LRR81 pKa = 11.84 MPSAPEE87 pKa = 3.47 AHH89 pKa = 7.55 DD90 pKa = 3.5 IPEE93 pKa = 4.31 DD94 pKa = 3.64 TNRR97 pKa = 11.84 FSTIYY102 pKa = 10.38 EE103 pKa = 3.78 VDD105 pKa = 3.67 EE106 pKa = 4.55 EE107 pKa = 4.46 CGEE110 pKa = 4.17 RR111 pKa = 11.84 AILSLNGEE119 pKa = 4.5 DD120 pKa = 3.65 TNEE123 pKa = 4.03 PTGHH127 pKa = 6.71 IEE129 pKa = 4.27 GEE131 pKa = 4.44 SGTATHH137 pKa = 6.04 QGEE140 pKa = 4.28 YY141 pKa = 10.21 TIDD144 pKa = 3.29 LGQINRR150 pKa = 11.84 DD151 pKa = 3.11 IKK153 pKa = 11.27 NSIQTFIEE161 pKa = 4.03 TGKK164 pKa = 9.81 RR165 pKa = 11.84 HH166 pKa = 6.48 PDD168 pKa = 2.71 ILAINPKK175 pKa = 10.59 LFMQNAAIIWGNSAVRR191 pKa = 11.84 GRR193 pKa = 11.84 ANISPISFIHH203 pKa = 5.67 LRR205 pKa = 11.84 SGISHH210 pKa = 7.86 PIKK213 pKa = 10.22 SWFFLGMMMASFNKK227 pKa = 9.66 KK228 pKa = 9.59 GKK230 pKa = 6.88 PTGRR234 pKa = 11.84 AFFVPHH240 pKa = 5.4 EE241 pKa = 4.25 QIMAISQKK249 pKa = 11.18 DD250 pKa = 3.37 MQNFNKK256 pKa = 9.75 VKK258 pKa = 10.53 HH259 pKa = 6.41 ILDD262 pKa = 3.73 KK263 pKa = 11.22 YY264 pKa = 11.13 KK265 pKa = 10.89 EE266 pKa = 4.15 DD267 pKa = 3.62 TRR269 pKa = 11.84 QSNIFIVDD277 pKa = 3.41 ITRR280 pKa = 11.84 EE281 pKa = 3.69 IVLNEE286 pKa = 3.59 KK287 pKa = 9.29 MKK289 pKa = 10.33 QFGVIRR295 pKa = 11.84 SYY297 pKa = 11.4 ALCVPKK303 pKa = 10.45 YY304 pKa = 9.57 HH305 pKa = 7.01 HH306 pKa = 7.2 LFPSSSLNTIFGQVQSGNIRR326 pKa = 11.84 MHH328 pKa = 6.6 IGEE331 pKa = 4.51 RR332 pKa = 11.84 EE333 pKa = 4.09 VIEE336 pKa = 4.82 DD337 pKa = 4.12 LNAKK341 pKa = 9.68 CIEE344 pKa = 4.29 YY345 pKa = 10.54 NLSDD349 pKa = 4.66 LPSDD353 pKa = 3.68 VLNGEE358 pKa = 4.14 RR359 pKa = 11.84 EE360 pKa = 3.88 EE361 pKa = 4.8 DD362 pKa = 3.11 KK363 pKa = 11.16 SNRR366 pKa = 11.84 YY367 pKa = 8.5 IQDD370 pKa = 4.13 DD371 pKa = 3.44 MDD373 pKa = 3.85 EE374 pKa = 4.24 DD375 pKa = 4.08 KK376 pKa = 11.02 KK377 pKa = 11.22 RR378 pKa = 11.84 KK379 pKa = 7.78 TNPTPITLNEE389 pKa = 4.01 LRR391 pKa = 11.84 RR392 pKa = 11.84 EE393 pKa = 3.92 LDD395 pKa = 3.88 SISQNQTQFQEE406 pKa = 4.29 IILLILKK413 pKa = 9.55 KK414 pKa = 10.21 QEE416 pKa = 3.64 EE417 pKa = 4.74 RR418 pKa = 11.84 IDD420 pKa = 4.14 KK421 pKa = 10.37 ISEE424 pKa = 3.76 ILGDD428 pKa = 3.92 VLSLQQKK435 pKa = 5.45 TTSQGEE441 pKa = 3.88 IWLGASIKK449 pKa = 10.3 AAEE452 pKa = 5.11 KK453 pKa = 10.02 ISQFEE458 pKa = 4.58 GNQEE462 pKa = 4.05 TYY464 pKa = 7.59 QTYY467 pKa = 11.01 LLTKK471 pKa = 10.28 IMIHH475 pKa = 5.81 LRR477 pKa = 11.84 EE478 pKa = 4.11 SEE480 pKa = 3.79 QRR482 pKa = 11.84 IIKK485 pKa = 7.72 EE486 pKa = 3.77 LCHH489 pKa = 6.46 FKK491 pKa = 10.49 TNPVRR496 pKa = 11.84 PEE498 pKa = 3.57 IPTPNLLLRR507 pKa = 11.84 RR508 pKa = 11.84 ALEE511 pKa = 4.12 TMTEE515 pKa = 4.04 EE516 pKa = 4.21 MSLHH520 pKa = 6.05 PKK522 pKa = 10.06 EE523 pKa = 5.96 NLIKK527 pKa = 9.44 WNPKK531 pKa = 6.23 ITEE534 pKa = 4.05 IVEE537 pKa = 4.36 SKK539 pKa = 10.85 DD540 pKa = 3.14 PEE542 pKa = 4.81 RR543 pKa = 11.84 INKK546 pKa = 7.3 MVRR549 pKa = 11.84 FMIQEE554 pKa = 4.0 RR555 pKa = 11.84 NKK557 pKa = 10.27 YY558 pKa = 9.13
Molecular weight: 63.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.253
IPC2_protein 5.232
IPC_protein 5.207
Toseland 5.245
ProMoST 5.372
Dawson 5.232
Bjellqvist 5.347
Wikipedia 5.105
Rodwell 5.169
Grimsley 5.194
Solomon 5.232
Lehninger 5.194
Nozaki 5.372
DTASelect 5.512
Thurlkill 5.258
EMBOSS 5.194
Sillero 5.461
Patrickios 4.355
IPC_peptide 5.245
IPC2_peptide 5.461
IPC2.peptide.svr19 5.431
Protein with the highest isoelectric point:
>tr|I3VIZ2|I3VIZ2_9MONO Putative attachment protein OS=Sunshine Coast virus OX=1195087 PE=4 SV=1
MM1 pKa = 7.66 EE2 pKa = 4.7 NQHH5 pKa = 7.62 IIIKK9 pKa = 9.97 KK10 pKa = 8.41 KK11 pKa = 10.61 KK12 pKa = 9.27 KK13 pKa = 9.58 GKK15 pKa = 9.65 KK16 pKa = 8.37 GKK18 pKa = 9.76 KK19 pKa = 8.55 NKK21 pKa = 9.79 KK22 pKa = 7.18 EE23 pKa = 3.82 RR24 pKa = 11.84 KK25 pKa = 9.23 KK26 pKa = 10.2 KK27 pKa = 9.94 GKK29 pKa = 9.76 RR30 pKa = 11.84 EE31 pKa = 3.74 KK32 pKa = 10.48 EE33 pKa = 3.97 RR34 pKa = 11.84 EE35 pKa = 4.17 KK36 pKa = 11.02 KK37 pKa = 9.02 STPKK41 pKa = 10.14 PQLKK45 pKa = 10.13 PKK47 pKa = 10.24 ILTNLRR53 pKa = 11.84 GDD55 pKa = 4.04 SPMEE59 pKa = 4.01 TPQLFSGRR67 pKa = 11.84 TGHH70 pKa = 5.1 QWSRR74 pKa = 11.84 RR75 pKa = 11.84 YY76 pKa = 9.58 IRR78 pKa = 11.84 YY79 pKa = 10.06 NYY81 pKa = 10.2 FLTLTLLTVIFADD94 pKa = 4.03 HH95 pKa = 6.98 QEE97 pKa = 4.05 LTQNSDD103 pKa = 3.59 IIVSTVALEE112 pKa = 4.07 TRR114 pKa = 11.84 VVSVQTGSSWFLLRR128 pKa = 11.84 VNTTFKK134 pKa = 11.21 NDD136 pKa = 3.33 GLTSRR141 pKa = 11.84 CTKK144 pKa = 10.35 LGEE147 pKa = 4.18 NFVSHH152 pKa = 6.24 VNEE155 pKa = 3.8 FQEE158 pKa = 4.44 NIKK161 pKa = 8.63 ITASSLIPKK170 pKa = 9.52 QAILEE175 pKa = 4.34 VNTAKK180 pKa = 10.92 NLVSALTRR188 pKa = 11.84 TTRR191 pKa = 11.84 SNRR194 pKa = 11.84 RR195 pKa = 11.84 KK196 pKa = 9.61 RR197 pKa = 11.84 LAGAAIAVAAVAISGAALVTAGIALAEE224 pKa = 4.21 TRR226 pKa = 11.84 TLAASVDD233 pKa = 4.19 SVISGAEE240 pKa = 3.81 KK241 pKa = 10.29 TNAALNQLTQTMKK254 pKa = 10.89 SFTKK258 pKa = 9.03 EE259 pKa = 3.93 TQDD262 pKa = 5.05 NISTLAKK269 pKa = 10.03 EE270 pKa = 3.66 ISEE273 pKa = 4.08 ANKK276 pKa = 10.55 RR277 pKa = 11.84 IDD279 pKa = 3.28 CLEE282 pKa = 4.2 YY283 pKa = 8.85 YY284 pKa = 10.21 TEE286 pKa = 4.27 VKK288 pKa = 10.68 NQFLDD293 pKa = 3.98 FTSYY297 pKa = 11.05 NYY299 pKa = 10.31 EE300 pKa = 3.71 ILQASMSKK308 pKa = 10.58 NGGIPLRR315 pKa = 11.84 LMKK318 pKa = 10.34 RR319 pKa = 11.84 IMVPNPQMTAQSNTIEE335 pKa = 4.19 EE336 pKa = 4.37 LVLRR340 pKa = 11.84 RR341 pKa = 11.84 GRR343 pKa = 11.84 IRR345 pKa = 11.84 LLAYY349 pKa = 8.73 TNSTLTYY356 pKa = 9.54 RR357 pKa = 11.84 IDD359 pKa = 3.56 VPRR362 pKa = 11.84 FASMGKK368 pKa = 9.68 ISPLLISVDD377 pKa = 3.87 EE378 pKa = 4.91 PISLIGSGIYY388 pKa = 9.99 TSILSVDD395 pKa = 4.05 LGIVLLDD402 pKa = 3.89 CEE404 pKa = 4.72 EE405 pKa = 4.94 DD406 pKa = 3.69 NISYY410 pKa = 10.06 LCSSSSPSDD419 pKa = 4.28 AIVKK423 pKa = 10.1 NCLKK427 pKa = 10.76 DD428 pKa = 3.47 STADD432 pKa = 3.89 CISALQEE439 pKa = 3.92 SGAKK443 pKa = 8.49 KK444 pKa = 10.2 GKK446 pKa = 9.7 YY447 pKa = 8.9 FVYY450 pKa = 10.54 QSGFLVSRR458 pKa = 11.84 CSQQVCTCLEE468 pKa = 3.87 PEE470 pKa = 3.53 IRR472 pKa = 11.84 RR473 pKa = 11.84 IYY475 pKa = 10.24 VSHH478 pKa = 7.42 PLSKK482 pKa = 9.79 EE483 pKa = 3.98 TSQCTQVSIKK493 pKa = 9.46 TQDD496 pKa = 3.01 QTFRR500 pKa = 11.84 LLLTSVKK507 pKa = 10.33 AAGIINIQAPTEE519 pKa = 4.2 IKK521 pKa = 10.32 RR522 pKa = 11.84 KK523 pKa = 4.99 TWKK526 pKa = 10.64 DD527 pKa = 2.75 IGLTFGKK534 pKa = 10.56 YY535 pKa = 5.87 NTKK538 pKa = 10.18 FEE540 pKa = 4.72 EE541 pKa = 4.14 IEE543 pKa = 3.95 EE544 pKa = 4.74 LIEE547 pKa = 4.18 NSNGDD552 pKa = 3.68 LEE554 pKa = 4.29 KK555 pKa = 11.06 ARR557 pKa = 11.84 TYY559 pKa = 11.13 LVLSQNLKK567 pKa = 9.37 VVVIVTSIVIAFLILFVIFILKK589 pKa = 10.31 RR590 pKa = 11.84 IYY592 pKa = 10.12 DD593 pKa = 3.91 LKK595 pKa = 11.29 NRR597 pKa = 11.84 VTKK600 pKa = 10.47 IEE602 pKa = 4.18 VYY604 pKa = 10.46 LEE606 pKa = 4.0 RR607 pKa = 6.05
Molecular weight: 68.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.0
IPC2_protein 8.975
IPC_protein 8.858
Toseland 9.765
ProMoST 9.443
Dawson 9.97
Bjellqvist 9.633
Wikipedia 10.101
Rodwell 10.452
Grimsley 10.028
Solomon 9.984
Lehninger 9.955
Nozaki 9.838
DTASelect 9.604
Thurlkill 9.823
EMBOSS 10.175
Sillero 9.897
Patrickios 9.736
IPC_peptide 9.984
IPC2_peptide 8.2
IPC2.peptide.svr19 7.891
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4847
361
2198
807.8
91.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.734 ± 0.998
1.754 ± 0.288
5.178 ± 0.389
6.932 ± 0.481
3.899 ± 0.272
5.178 ± 0.603
1.795 ± 0.282
9.263 ± 0.422
7.18 ± 0.49
10.543 ± 0.64
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.496 ± 0.241
5.178 ± 0.348
4.312 ± 0.523
3.59 ± 0.333
5.426 ± 0.224
8.686 ± 0.358
5.756 ± 0.486
5.137 ± 0.287
0.825 ± 0.086
3.136 ± 0.299
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here