Drancourtella sp. An177
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2789 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y4M0A3|A0A1Y4M0A3_9FIRM MerR family transcriptional regulator OS=Drancourtella sp. An177 OX=1965573 GN=B5F13_13740 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 9.89 KK3 pKa = 9.52 KK4 pKa = 10.11 HH5 pKa = 6.68 CITVLAAVLTVGLMGGILAGCGSRR29 pKa = 11.84 RR30 pKa = 11.84 QVQEE34 pKa = 3.55 PQKK37 pKa = 10.39 QQSQTNEE44 pKa = 3.58 KK45 pKa = 10.65 SGVICLKK52 pKa = 10.29 VNPEE56 pKa = 3.56 IAVSYY61 pKa = 10.38 DD62 pKa = 3.33 EE63 pKa = 5.1 NGMVTKK69 pKa = 10.82 VEE71 pKa = 3.93 GRR73 pKa = 11.84 NDD75 pKa = 3.57 DD76 pKa = 3.81 GKK78 pKa = 11.3 KK79 pKa = 10.59 VIAQYY84 pKa = 10.73 EE85 pKa = 4.8 GYY87 pKa = 9.71 EE88 pKa = 4.32 GKK90 pKa = 10.34 ACRR93 pKa = 11.84 DD94 pKa = 3.73 VISEE98 pKa = 4.33 LVAKK102 pKa = 10.45 INDD105 pKa = 3.15 AGYY108 pKa = 9.71 FVEE111 pKa = 5.28 EE112 pKa = 3.88 TDD114 pKa = 4.23 QEE116 pKa = 4.4 KK117 pKa = 10.72 KK118 pKa = 10.75 QITLEE123 pKa = 4.05 IEE125 pKa = 4.12 AGSYY129 pKa = 10.88 LPGDD133 pKa = 4.29 DD134 pKa = 3.58 FLKK137 pKa = 11.14 NIVAGVQEE145 pKa = 4.29 YY146 pKa = 7.78 TASKK150 pKa = 10.11 QLPNSVAVEE159 pKa = 4.18 GEE161 pKa = 4.43 SNYY164 pKa = 10.56 GWTNYY169 pKa = 10.33 GDD171 pKa = 3.61 TDD173 pKa = 3.92 YY174 pKa = 11.68 GPDD177 pKa = 3.42 NDD179 pKa = 5.65 GVTDD183 pKa = 4.37 YY184 pKa = 11.62 NDD186 pKa = 3.31 TDD188 pKa = 3.76 YY189 pKa = 11.68 GPNNDD194 pKa = 4.65 GVTDD198 pKa = 4.02 YY199 pKa = 11.63 NDD201 pKa = 3.31 TDD203 pKa = 3.76 YY204 pKa = 11.68 GPNNDD209 pKa = 4.91 GVTDD213 pKa = 3.92 YY214 pKa = 11.67 NNTDD218 pKa = 3.42 YY219 pKa = 11.46 GPNNDD224 pKa = 4.65 GVTDD228 pKa = 4.02 YY229 pKa = 11.76 NDD231 pKa = 3.33 TDD233 pKa = 3.66 YY234 pKa = 9.83 GTNNDD239 pKa = 3.15 GVTDD243 pKa = 4.01 YY244 pKa = 11.06 SAPAQNPAPSTPAPSTGGDD263 pKa = 2.93 SGYY266 pKa = 11.02 DD267 pKa = 3.7 DD268 pKa = 5.41 GNSGYY273 pKa = 10.82 DD274 pKa = 3.76 GGDD277 pKa = 2.91 SGYY280 pKa = 11.24 DD281 pKa = 3.34 DD282 pKa = 4.26
Molecular weight: 30.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.808
IPC_protein 3.834
Toseland 3.605
ProMoST 3.973
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.503
Solomon 3.821
Lehninger 3.783
Nozaki 3.935
DTASelect 4.215
Thurlkill 3.656
EMBOSS 3.783
Sillero 3.961
Patrickios 0.617
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.846
Protein with the highest isoelectric point:
>tr|A0A1Y4M3A9|A0A1Y4M3A9_9FIRM Uncharacterized protein OS=Drancourtella sp. An177 OX=1965573 GN=B5F13_11055 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 8.72 MTFQPKK8 pKa = 8.86 KK9 pKa = 8.2 RR10 pKa = 11.84 SRR12 pKa = 11.84 AKK14 pKa = 9.27 VHH16 pKa = 5.81 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTAGGRR28 pKa = 11.84 KK29 pKa = 8.71 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.18 GRR39 pKa = 11.84 KK40 pKa = 8.18 QLSAA44 pKa = 3.9
Molecular weight: 4.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.111
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.647
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.369
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.095
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2789
0
2789
871670
29
1996
312.5
35.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.2 ± 0.049
1.504 ± 0.019
5.519 ± 0.038
8.203 ± 0.047
4.174 ± 0.029
7.122 ± 0.039
1.791 ± 0.022
7.396 ± 0.041
7.247 ± 0.04
8.806 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.182 ± 0.025
3.918 ± 0.028
3.33 ± 0.028
3.251 ± 0.022
4.851 ± 0.042
5.506 ± 0.032
5.219 ± 0.032
6.731 ± 0.04
0.919 ± 0.015
4.131 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here