Hubei virga-like virus 12

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJX5|A0A1L3KJX5_9VIRU RdRp catalytic domain-containing protein OS=Hubei virga-like virus 12 OX=1923327 PE=4 SV=1
MM1 pKa = 7.59NEE3 pKa = 4.14DD4 pKa = 3.14QDD6 pKa = 4.06IVNIDD11 pKa = 3.82DD12 pKa = 4.15LLIGGGHH19 pKa = 6.74EE20 pKa = 4.18DD21 pKa = 3.26TRR23 pKa = 11.84TLRR26 pKa = 11.84FVKK29 pKa = 10.52NLEE32 pKa = 4.21TTVKK36 pKa = 9.75SFKK39 pKa = 10.78YY40 pKa = 10.15KK41 pKa = 10.06LLKK44 pKa = 10.29RR45 pKa = 11.84QFGHH49 pKa = 5.6EE50 pKa = 4.16TFKK53 pKa = 11.41DD54 pKa = 3.78LVEE57 pKa = 6.08DD58 pKa = 4.36DD59 pKa = 4.6VDD61 pKa = 5.7DD62 pKa = 4.53GMEE65 pKa = 4.63LIEE68 pKa = 6.63DD69 pKa = 3.49ITKK72 pKa = 10.42QLLIDD77 pKa = 3.95NKK79 pKa = 10.35VKK81 pKa = 9.73EE82 pKa = 4.11TDD84 pKa = 3.88KK85 pKa = 11.27RR86 pKa = 11.84IICNNLKK93 pKa = 10.42KK94 pKa = 10.2ISKK97 pKa = 9.89KK98 pKa = 10.53FYY100 pKa = 9.45NCYY103 pKa = 9.89QSFIMKK109 pKa = 9.61IHH111 pKa = 6.65NKK113 pKa = 10.17LEE115 pKa = 4.06YY116 pKa = 10.27NDD118 pKa = 4.87RR119 pKa = 11.84NFNSTYY125 pKa = 9.7NTTFDD130 pKa = 3.59FFKK133 pKa = 10.46FAAYY137 pKa = 10.56LIYY140 pKa = 10.91NDD142 pKa = 3.23MKK144 pKa = 11.32YY145 pKa = 10.17RR146 pKa = 11.84GSEE149 pKa = 4.0NAHH152 pKa = 4.96VTTKK156 pKa = 10.48IKK158 pKa = 10.57FNYY161 pKa = 8.15QDD163 pKa = 3.54KK164 pKa = 9.4PNTYY168 pKa = 8.46TSHH171 pKa = 6.43MINYY175 pKa = 6.73KK176 pKa = 8.83TEE178 pKa = 3.97YY179 pKa = 9.94KK180 pKa = 10.11PNKK183 pKa = 8.84PVVKK187 pKa = 10.24HH188 pKa = 5.56EE189 pKa = 4.46EE190 pKa = 4.03IIKK193 pKa = 8.41NTKK196 pKa = 9.94DD197 pKa = 3.04LFRR200 pKa = 11.84NEE202 pKa = 3.52ISLTNNIIQNKK213 pKa = 8.0LQEE216 pKa = 4.53EE217 pKa = 4.27DD218 pKa = 5.19SEE220 pKa = 4.55DD221 pKa = 3.65TDD223 pKa = 5.25DD224 pKa = 6.65DD225 pKa = 3.86ISEE228 pKa = 4.23SSGFIDD234 pKa = 5.39DD235 pKa = 5.74DD236 pKa = 5.13NITSTASVEE245 pKa = 4.24LSISPIDD252 pKa = 3.84EE253 pKa = 4.1SDD255 pKa = 3.75VEE257 pKa = 3.91IDD259 pKa = 3.98EE260 pKa = 4.74IEE262 pKa = 4.32DD263 pKa = 3.62MEE265 pKa = 4.91EE266 pKa = 3.81NTVNTTTLEE275 pKa = 4.02MEE277 pKa = 4.47EE278 pKa = 4.29NSVLSSNIEE287 pKa = 3.57ILDD290 pKa = 3.5NKK292 pKa = 10.08KK293 pKa = 10.02IRR295 pKa = 11.84DD296 pKa = 3.61EE297 pKa = 4.34LNFVFFSNTMNLYY310 pKa = 6.86TTLEE314 pKa = 4.12TSSIPNYY321 pKa = 10.2QLVFSNDD328 pKa = 3.35IKK330 pKa = 11.01CKK332 pKa = 10.68NYY334 pKa = 9.68TKK336 pKa = 11.03LNIEE340 pKa = 3.87NLYY343 pKa = 10.06YY344 pKa = 10.98DD345 pKa = 3.97KK346 pKa = 10.75DD347 pKa = 4.14YY348 pKa = 11.56KK349 pKa = 10.48KK350 pKa = 10.68LYY352 pKa = 9.81YY353 pKa = 10.56DD354 pKa = 3.78VDD356 pKa = 3.12ITNFNKK362 pKa = 9.92FLDD365 pKa = 3.93MLSIIIDD372 pKa = 4.32DD373 pKa = 4.97IINKK377 pKa = 9.38YY378 pKa = 10.89GNINSLFKK386 pKa = 10.94LPYY389 pKa = 10.12NPFYY393 pKa = 10.83DD394 pKa = 3.92YY395 pKa = 11.15QRR397 pKa = 11.84LLILSRR403 pKa = 11.84NIINKK408 pKa = 9.58HH409 pKa = 4.5EE410 pKa = 5.09LINIAIIIPNEE421 pKa = 4.26SKK423 pKa = 11.3VNTLKK428 pKa = 10.37QHH430 pKa = 6.57LNYY433 pKa = 10.51LLPQTPSKK441 pKa = 10.67HH442 pKa = 6.11KK443 pKa = 10.27DD444 pKa = 3.26YY445 pKa = 11.52SSFLSRR451 pKa = 11.84DD452 pKa = 3.31EE453 pKa = 4.95LIKK456 pKa = 10.86KK457 pKa = 8.52EE458 pKa = 4.04INDD461 pKa = 3.42YY462 pKa = 9.33TVALVSEE469 pKa = 4.4NNMNYY474 pKa = 10.46KK475 pKa = 9.77ILNAMNEE482 pKa = 4.08SALKK486 pKa = 10.48YY487 pKa = 10.43KK488 pKa = 8.94ITKK491 pKa = 10.12LVITNNCANIYY502 pKa = 9.57TMYY505 pKa = 9.56NTGSTVIGCTIPGNEE520 pKa = 4.51KK521 pKa = 9.2YY522 pKa = 9.21TFVKK526 pKa = 8.53NTKK529 pKa = 9.15IDD531 pKa = 3.66NPHH534 pKa = 6.07MYY536 pKa = 10.43GYY538 pKa = 10.37DD539 pKa = 3.06GKK541 pKa = 11.16ALVEE545 pKa = 4.33YY546 pKa = 10.43NFKK549 pKa = 9.72TEE551 pKa = 3.91RR552 pKa = 11.84YY553 pKa = 5.93EE554 pKa = 4.38TNSNTILISKK564 pKa = 6.36QTKK567 pKa = 10.12LIQDD571 pKa = 3.14QKK573 pKa = 10.4IYY575 pKa = 11.39NNIEE579 pKa = 4.06EE580 pKa = 4.26YY581 pKa = 10.92LKK583 pKa = 10.0TEE585 pKa = 4.3LYY587 pKa = 10.3IPIIYY592 pKa = 9.21YY593 pKa = 9.77VNGVAGCGKK602 pKa = 7.28TQNIISNFDD611 pKa = 3.39INDD614 pKa = 3.53DD615 pKa = 4.45FIISTTKK622 pKa = 10.86ANTEE626 pKa = 3.86EE627 pKa = 4.51LIEE630 pKa = 5.02RR631 pKa = 11.84ISKK634 pKa = 9.79HH635 pKa = 4.77YY636 pKa = 9.82KK637 pKa = 8.06IKK639 pKa = 10.81KK640 pKa = 8.51EE641 pKa = 3.93IIISRR646 pKa = 11.84VKK648 pKa = 10.19TIDD651 pKa = 3.16SVLINKK657 pKa = 7.84PRR659 pKa = 11.84QNIKK663 pKa = 10.53KK664 pKa = 10.09LFIDD668 pKa = 4.78EE669 pKa = 4.34IFMVHH674 pKa = 7.04AGTIMFAIQILKK686 pKa = 9.57PKK688 pKa = 10.05YY689 pKa = 9.93VLALGDD695 pKa = 3.9TKK697 pKa = 10.66QIPYY701 pKa = 9.94IEE703 pKa = 4.41RR704 pKa = 11.84LALIPNNYY712 pKa = 9.95SSIIDD717 pKa = 3.26ISVKK721 pKa = 10.7LEE723 pKa = 3.74NLNVSSRR730 pKa = 11.84CPADD734 pKa = 3.21VVEE737 pKa = 4.81IFKK740 pKa = 10.91NDD742 pKa = 3.4YY743 pKa = 9.7KK744 pKa = 11.26DD745 pKa = 3.2SGGFYY750 pKa = 7.03TTNGRR755 pKa = 11.84TRR757 pKa = 11.84SVRR760 pKa = 11.84IQNFISKK767 pKa = 10.53DD768 pKa = 3.64NLKK771 pKa = 10.83SLIDD775 pKa = 3.84EE776 pKa = 4.3EE777 pKa = 4.83TMVICFKK784 pKa = 10.77EE785 pKa = 3.75ADD787 pKa = 3.64AEE789 pKa = 4.1EE790 pKa = 5.11LILYY794 pKa = 9.69GIDD797 pKa = 3.62AYY799 pKa = 9.8TIHH802 pKa = 7.23KK803 pKa = 9.75KK804 pKa = 10.38QGTTKK809 pKa = 10.39KK810 pKa = 10.45KK811 pKa = 9.97VILVRR816 pKa = 11.84LSVKK820 pKa = 10.25PNEE823 pKa = 4.36EE824 pKa = 3.6IFKK827 pKa = 10.54KK828 pKa = 10.53RR829 pKa = 11.84EE830 pKa = 3.86QILVALTRR838 pKa = 11.84HH839 pKa = 5.35TEE841 pKa = 3.6QFIYY845 pKa = 8.87YY846 pKa = 8.17TKK848 pKa = 10.02LTSDD852 pKa = 4.58DD853 pKa = 3.47VCKK856 pKa = 10.73CINKK860 pKa = 9.19VNNTPLTTIMKK871 pKa = 10.46NIVTQDD877 pKa = 3.64KK878 pKa = 10.42IKK880 pKa = 10.73KK881 pKa = 8.55LQLPACMSEE890 pKa = 4.4KK891 pKa = 10.68LNEE894 pKa = 4.26LKK896 pKa = 10.95GGANEE901 pKa = 4.29NFWKK905 pKa = 10.43RR906 pKa = 11.84ASKK909 pKa = 10.55KK910 pKa = 9.39IRR912 pKa = 11.84KK913 pKa = 8.92KK914 pKa = 10.5KK915 pKa = 10.1

Molecular weight: 106.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KK50|A0A1L3KK50_9VIRU Uncharacterized protein OS=Hubei virga-like virus 12 OX=1923327 PE=4 SV=1
MM1 pKa = 7.44YY2 pKa = 10.3ILLFFILFYY11 pKa = 11.37NFIFEE16 pKa = 4.58PCGKK20 pKa = 5.91TTKK23 pKa = 8.56PTPALNDD30 pKa = 3.06VCVFYY35 pKa = 11.05CPSINGFQINKK46 pKa = 9.8KK47 pKa = 10.33LYY49 pKa = 10.38LDD51 pKa = 4.12GKK53 pKa = 9.38GHH55 pKa = 6.12SLHH58 pKa = 6.3GFWYY62 pKa = 10.21KK63 pKa = 9.51EE64 pKa = 3.68LKK66 pKa = 10.85DD67 pKa = 3.48PVEE70 pKa = 4.69RR71 pKa = 11.84ISFNDD76 pKa = 3.66FLQKK80 pKa = 10.27KK81 pKa = 9.26PKK83 pKa = 9.67FKK85 pKa = 11.07YY86 pKa = 9.73IFYY89 pKa = 10.63DD90 pKa = 3.33STTLLDD96 pKa = 4.1EE97 pKa = 5.15LGCGQYY103 pKa = 9.97RR104 pKa = 11.84EE105 pKa = 4.34YY106 pKa = 10.89VKK108 pKa = 10.81HH109 pKa = 5.73GAKK112 pKa = 9.88SGYY115 pKa = 9.17TYY117 pKa = 10.43LQWLNKK123 pKa = 9.64INEE126 pKa = 4.52CQVPTFPHH134 pKa = 5.21QCHH137 pKa = 6.42FNGSHH142 pKa = 5.86NVILSISNYY151 pKa = 9.33NISTIGCKK159 pKa = 10.21LNYY162 pKa = 9.7PFINLCRR169 pKa = 11.84FF170 pKa = 3.39

Molecular weight: 19.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2587

170

915

431.2

50.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.667 ± 0.358

1.701 ± 0.411

6.301 ± 0.688

5.18 ± 0.847

6.687 ± 1.301

2.629 ± 0.394

1.778 ± 0.355

11.365 ± 0.577

9.084 ± 1.072

9.818 ± 0.658

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.392 ± 0.315

9.934 ± 0.642

2.783 ± 0.301

2.551 ± 0.131

3.247 ± 0.325

6.301 ± 0.539

6.107 ± 0.498

3.943 ± 0.343

0.734 ± 0.355

5.798 ± 0.347
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski