Roseovarius faecimaris

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseovarius

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3738 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I6IQ10|A0A6I6IQ10_9RHOB 6-phosphogluconolactonase OS=Roseovarius faecimaris OX=2494550 GN=pgl PE=3 SV=1
MM1 pKa = 8.12SIMTVSSNEE10 pKa = 3.51QLMIEE15 pKa = 4.49LVNSFRR21 pKa = 11.84LDD23 pKa = 3.52PYY25 pKa = 11.2GEE27 pKa = 3.99AVRR30 pKa = 11.84IGLTSQEE37 pKa = 3.83IAALGLPQGSASPLAGTYY55 pKa = 7.47TLHH58 pKa = 6.17VAAGQHH64 pKa = 5.25SSWMIANDD72 pKa = 3.87DD73 pKa = 4.13FSHH76 pKa = 6.38TGAGGSSAGQRR87 pKa = 11.84MEE89 pKa = 3.84DD90 pKa = 2.93AGYY93 pKa = 10.09VFSGSYY99 pKa = 9.6HH100 pKa = 5.43WGEE103 pKa = 4.24NIGYY107 pKa = 8.23RR108 pKa = 11.84ASSGTLDD115 pKa = 3.34TTQAIYY121 pKa = 10.6HH122 pKa = 6.58LNRR125 pKa = 11.84ALILSDD131 pKa = 2.77GHH133 pKa = 6.67RR134 pKa = 11.84ANLLNPVFRR143 pKa = 11.84EE144 pKa = 4.03VGIGQANGDD153 pKa = 3.91YY154 pKa = 10.59LGYY157 pKa = 9.92DD158 pKa = 3.06ATMITQNFATSNGATFLTGVVYY180 pKa = 11.09NDD182 pKa = 3.15INGDD186 pKa = 3.53DD187 pKa = 4.37FYY189 pKa = 11.79SVGEE193 pKa = 4.17GVGGVTFSLSGKK205 pKa = 10.34SDD207 pKa = 3.26TTYY210 pKa = 11.12GAGGYY215 pKa = 7.25SVKK218 pKa = 9.39VTSGGTQTVTIAYY231 pKa = 6.71GTTEE235 pKa = 3.46ISAAVHH241 pKa = 6.08IGSEE245 pKa = 4.33NVKK248 pKa = 10.5LDD250 pKa = 4.85LIDD253 pKa = 3.63EE254 pKa = 4.57TVLRR258 pKa = 11.84SSSSLTLLSGATDD271 pKa = 3.36AVLLGLEE278 pKa = 4.4DD279 pKa = 3.75LSLYY283 pKa = 10.79GNSRR287 pKa = 11.84HH288 pKa = 6.06NHH290 pKa = 4.48LTGNAGDD297 pKa = 3.79NMLAGGLGNDD307 pKa = 4.64TIDD310 pKa = 3.65GGAGVDD316 pKa = 3.55TVKK319 pKa = 10.69INAGSSEE326 pKa = 4.19VTVEE330 pKa = 3.81ISGNDD335 pKa = 3.46IIVTSAEE342 pKa = 3.94GTDD345 pKa = 3.38VYY347 pKa = 11.39RR348 pKa = 11.84NVEE351 pKa = 4.06FFSFSNQTLTSSEE364 pKa = 3.91VRR366 pKa = 11.84EE367 pKa = 4.21LDD369 pKa = 3.47PSNSTTSSNATSPGPSSSTTGLFIAGGDD397 pKa = 3.92GADD400 pKa = 4.53LLDD403 pKa = 3.87GSNYY407 pKa = 9.92NDD409 pKa = 3.65SLKK412 pKa = 11.04GGNGNDD418 pKa = 3.51TLSGYY423 pKa = 10.86GGDD426 pKa = 4.23DD427 pKa = 3.57NLPGGEE433 pKa = 4.73GDD435 pKa = 4.01DD436 pKa = 3.89QVSGGAGNDD445 pKa = 3.67LLGGGLGDD453 pKa = 3.82DD454 pKa = 4.37TLNAGAGNDD463 pKa = 3.44QGGGGEE469 pKa = 4.38GNDD472 pKa = 3.73VINAGEE478 pKa = 4.17GDD480 pKa = 4.47DD481 pKa = 4.26IFSGGAGNDD490 pKa = 3.52LVQGADD496 pKa = 3.27GHH498 pKa = 6.64DD499 pKa = 3.48WLAASYY505 pKa = 11.61GNDD508 pKa = 3.25TVTGGAGNDD517 pKa = 3.91TIGAGHH523 pKa = 6.05GHH525 pKa = 6.62DD526 pKa = 5.09RR527 pKa = 11.84INGGAGDD534 pKa = 4.04DD535 pKa = 3.63QIGAGNHH542 pKa = 7.12DD543 pKa = 4.26DD544 pKa = 3.97TLLGGDD550 pKa = 4.13GNDD553 pKa = 3.93FLGGGYY559 pKa = 10.45GRR561 pKa = 11.84DD562 pKa = 3.72VLDD565 pKa = 4.69GEE567 pKa = 5.04AGQDD571 pKa = 3.7TLNGGYY577 pKa = 10.64GSDD580 pKa = 3.37TLTGGVGQDD589 pKa = 2.75EE590 pKa = 4.92FVFNTLSGNGNDD602 pKa = 4.27VITDD606 pKa = 3.8FQVGIDD612 pKa = 3.83HH613 pKa = 6.88IKK615 pKa = 10.83LVGLDD620 pKa = 3.69LGHH623 pKa = 6.97NPMQNLDD630 pKa = 3.42IRR632 pKa = 11.84DD633 pKa = 3.85TANGAVIEE641 pKa = 4.43YY642 pKa = 10.49GDD644 pKa = 3.66FTITVSGVSSHH655 pKa = 6.63SLTADD660 pKa = 3.26DD661 pKa = 6.7FIFF664 pKa = 4.65

Molecular weight: 67.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I6IRU3|A0A6I6IRU3_9RHOB Ribosome-binding factor A OS=Roseovarius faecimaris OX=2494550 GN=rbfA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3738

0

3738

1185881

28

3335

317.3

34.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.115 ± 0.059

0.983 ± 0.012

6.029 ± 0.04

6.157 ± 0.039

3.703 ± 0.025

8.724 ± 0.05

2.166 ± 0.023

5.143 ± 0.028

3.154 ± 0.034

10.037 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.884 ± 0.021

2.586 ± 0.027

5.11 ± 0.027

3.222 ± 0.022

6.562 ± 0.045

5.11 ± 0.029

5.45 ± 0.042

7.081 ± 0.031

1.432 ± 0.016

2.353 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski