Paenibacillus larvae subsp. larvae
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4640 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6C0QUR0|A0A6C0QUR0_9BACL Sorbose PTS system IIB-like component OS=Paenibacillus larvae subsp. larvae OX=147375 GN=ERICV_03384 PE=4 SV=1
MM1 pKa = 7.88 PEE3 pKa = 4.73 LDD5 pKa = 4.0 RR6 pKa = 11.84 FDD8 pKa = 5.77 YY9 pKa = 11.22 DD10 pKa = 3.53 DD11 pKa = 4.31 TDD13 pKa = 5.33 PIVASCASCDD23 pKa = 3.55 GEE25 pKa = 4.35 IYY27 pKa = 10.71 EE28 pKa = 4.68 GDD30 pKa = 3.85 SVVSTTEE37 pKa = 3.35 GDD39 pKa = 3.56 IVHH42 pKa = 6.53 EE43 pKa = 4.26 EE44 pKa = 4.11 CFAAFARR51 pKa = 11.84 EE52 pKa = 4.34 TYY54 pKa = 10.21 RR55 pKa = 11.84 SVSGMIDD62 pKa = 2.89 AKK64 pKa = 10.98 GRR66 pKa = 11.84 III68 pKa = 4.77
Molecular weight: 7.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.768
IPC2_protein 3.948
IPC_protein 3.872
Toseland 3.668
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.592
Solomon 3.846
Lehninger 3.795
Nozaki 3.986
DTASelect 4.19
Thurlkill 3.732
EMBOSS 3.808
Sillero 3.999
Patrickios 1.901
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.889
Protein with the highest isoelectric point:
>tr|A0A2L1TUT2|A0A2L1TUT2_9BACL Ribosomal RNA small subunit methyltransferase I OS=Paenibacillus larvae subsp. larvae OX=147375 GN=rsmI PE=3 SV=1
MM1 pKa = 7.88 RR2 pKa = 11.84 PTFNPNNRR10 pKa = 11.84 KK11 pKa = 9.34 RR12 pKa = 11.84 KK13 pKa = 8.34 KK14 pKa = 9.1 NHH16 pKa = 4.74 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 10.03 NGRR28 pKa = 11.84 KK29 pKa = 8.83 VLSARR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.26 GRR39 pKa = 11.84 KK40 pKa = 8.56 VLSAA44 pKa = 4.05
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.676
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.398
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.125
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4640
0
4640
1256144
29
5369
270.7
30.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.233 ± 0.041
0.899 ± 0.012
5.036 ± 0.028
7.191 ± 0.048
4.184 ± 0.034
7.103 ± 0.034
2.208 ± 0.022
7.178 ± 0.041
6.622 ± 0.042
9.877 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.825 ± 0.019
3.958 ± 0.026
3.896 ± 0.023
3.947 ± 0.033
4.927 ± 0.037
6.057 ± 0.03
5.278 ± 0.024
6.907 ± 0.028
1.154 ± 0.015
3.52 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here