Listeria phage P40
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B6D7I9|B6D7I9_9CAUD Gp16 OS=Listeria phage P40 OX=560178 PE=4 SV=1
MM1 pKa = 7.46 IPKK4 pKa = 10.3 KK5 pKa = 9.82 LTLINGRR12 pKa = 11.84 GDD14 pKa = 3.54 ILPLNDD20 pKa = 3.89 NGSTGLFVLEE30 pKa = 4.11 WSGLGVNINFDD41 pKa = 3.69 LDD43 pKa = 3.55 KK44 pKa = 10.8 YY45 pKa = 10.29 QASQIVKK52 pKa = 10.04 NRR54 pKa = 11.84 TLDD57 pKa = 3.34 MQNFVVNIKK66 pKa = 10.3 IGLNYY71 pKa = 10.39 SATTPRR77 pKa = 11.84 QIYY80 pKa = 10.23 AGLIQFLNHH89 pKa = 6.02 TPYY92 pKa = 10.55 TLQSEE97 pKa = 4.56 DD98 pKa = 3.84 DD99 pKa = 3.6 AGTYY103 pKa = 10.3 LRR105 pKa = 11.84 DD106 pKa = 3.6 VQLQTMTLTDD116 pKa = 3.98 LKK118 pKa = 10.29 EE119 pKa = 4.0 GQIFEE124 pKa = 4.36 EE125 pKa = 4.35 TLEE128 pKa = 4.64 FICTSLWYY136 pKa = 10.73 SEE138 pKa = 5.7 DD139 pKa = 3.18 IYY141 pKa = 10.97 EE142 pKa = 4.22 YY143 pKa = 10.36 TPQDD147 pKa = 3.48 LSIPADD153 pKa = 3.41 TGKK156 pKa = 10.24 VFDD159 pKa = 4.91 KK160 pKa = 11.52 NEE162 pKa = 3.92 STTVKK167 pKa = 10.47 NQGGYY172 pKa = 10.9 GYY174 pKa = 10.86 LLPTDD179 pKa = 4.27 VNIAPYY185 pKa = 10.38 EE186 pKa = 3.96 PVGNGAITPDD196 pKa = 3.96 MYY198 pKa = 11.26 EE199 pKa = 4.47 LGSPTITGAFLDD211 pKa = 3.69 IDD213 pKa = 4.01 EE214 pKa = 5.56 LNVTFQAKK222 pKa = 10.05 VYY224 pKa = 10.99 LNGTLLFTGGTFDD237 pKa = 3.99 NDD239 pKa = 3.23 SGLFTINVPVTPVIGFNDD257 pKa = 3.59 IVRR260 pKa = 11.84 LDD262 pKa = 4.21 LVTQISPDD270 pKa = 3.58 TVLDD274 pKa = 3.95 TNNVPLAQYY283 pKa = 10.19 RR284 pKa = 11.84 YY285 pKa = 9.08 GYY287 pKa = 10.25 IYY289 pKa = 10.62 GITPNDD295 pKa = 3.08 VKK297 pKa = 11.41 GIFNVNNDD305 pKa = 3.74 SVYY308 pKa = 10.44 MGLTDD313 pKa = 4.95 GSPCIITAYY322 pKa = 10.48 DD323 pKa = 3.73 PVSGITNPAVSVTVDD338 pKa = 3.33 GNITQSDD345 pKa = 4.61 RR346 pKa = 11.84 YY347 pKa = 10.2 VISIPNDD354 pKa = 3.39 YY355 pKa = 10.25 AWEE358 pKa = 4.11 VSSIDD363 pKa = 3.1 GTAYY367 pKa = 9.76 CVRR370 pKa = 11.84 YY371 pKa = 10.19 NRR373 pKa = 11.84 TTGVFEE379 pKa = 6.02 NIYY382 pKa = 10.21 QYY384 pKa = 11.29 QDD386 pKa = 3.03 QTKK389 pKa = 9.05 TNFIKK394 pKa = 10.63 IPVGDD399 pKa = 3.77 SQVRR403 pKa = 11.84 FGGINFDD410 pKa = 4.1 NPGSYY415 pKa = 10.9 AKK417 pKa = 10.22 IIVRR421 pKa = 11.84 RR422 pKa = 11.84 EE423 pKa = 3.62 RR424 pKa = 11.84 LSIGG428 pKa = 3.23
Molecular weight: 47.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.891
IPC2_protein 4.164
IPC_protein 4.164
Toseland 3.948
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.088
Rodwell 3.986
Grimsley 3.859
Solomon 4.151
Lehninger 4.113
Nozaki 4.266
DTASelect 4.52
Thurlkill 3.986
EMBOSS 4.101
Sillero 4.291
Patrickios 1.43
IPC_peptide 4.151
IPC2_peptide 4.266
IPC2.peptide.svr19 4.168
Protein with the highest isoelectric point:
>tr|B6D7J8|B6D7J8_9CAUD Gp25 OS=Listeria phage P40 OX=560178 PE=4 SV=1
MM1 pKa = 7.52 VVILTFFIVGMCVGMFNVYY20 pKa = 10.18 HH21 pKa = 5.63 YY22 pKa = 10.03 VAKK25 pKa = 9.95 PALQEE30 pKa = 3.76 LRR32 pKa = 11.84 LLKK35 pKa = 10.78 GEE37 pKa = 4.51 PLPKK41 pKa = 9.47 RR42 pKa = 11.84 AKK44 pKa = 10.23 FSIKK48 pKa = 8.74 ITRR51 pKa = 3.45
Molecular weight: 5.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.222
IPC2_protein 9.589
IPC_protein 9.692
Toseland 10.365
ProMoST 10.189
Dawson 10.496
Bjellqvist 10.131
Wikipedia 10.628
Rodwell 11.082
Grimsley 10.54
Solomon 10.54
Lehninger 10.526
Nozaki 10.35
DTASelect 10.116
Thurlkill 10.365
EMBOSS 10.745
Sillero 10.409
Patrickios 10.906
IPC_peptide 10.54
IPC2_peptide 8.887
IPC2.peptide.svr19 8.507
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
62
0
62
11025
43
668
177.8
20.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.56 ± 0.353
0.744 ± 0.087
6.567 ± 0.316
7.138 ± 0.464
4.218 ± 0.248
6.113 ± 0.447
1.723 ± 0.177
7.356 ± 0.235
8.19 ± 0.471
8.444 ± 0.329
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.821 ± 0.27
5.905 ± 0.293
3.338 ± 0.213
3.42 ± 0.192
4.127 ± 0.274
5.651 ± 0.214
6.24 ± 0.329
6.948 ± 0.262
1.07 ± 0.15
4.426 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here