Amino acid dipepetide frequency for Listeria phage P40

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.816AlaAla: 0.816 ± 0.475
0.091AlaCys: 0.091 ± 0.087
3.538AlaAsp: 3.538 ± 0.656
3.719AlaGlu: 3.719 ± 0.682
2.903AlaPhe: 2.903 ± 0.526
2.903AlaGly: 2.903 ± 0.588
0.635AlaHis: 0.635 ± 0.19
5.261AlaIle: 5.261 ± 0.822
4.898AlaLys: 4.898 ± 0.718
5.261AlaLeu: 5.261 ± 0.696
2.449AlaMet: 2.449 ± 0.473
4.354AlaAsn: 4.354 ± 0.657
2.631AlaPro: 2.631 ± 0.746
1.814AlaGln: 1.814 ± 0.441
1.361AlaArg: 1.361 ± 0.37
3.447AlaSer: 3.447 ± 0.408
3.266AlaThr: 3.266 ± 0.613
3.356AlaVal: 3.356 ± 0.651
0.454AlaTrp: 0.454 ± 0.159
2.449AlaTyr: 2.449 ± 0.545
0.0AlaXaa: 0.0 ± 0.0
Cys
0.181CysAla: 0.181 ± 0.12
0.091CysCys: 0.091 ± 0.107
0.726CysAsp: 0.726 ± 0.365
0.635CysGlu: 0.635 ± 0.202
0.272CysPhe: 0.272 ± 0.164
0.272CysGly: 0.272 ± 0.183
0.272CysHis: 0.272 ± 0.154
0.454CysIle: 0.454 ± 0.208
0.454CysLys: 0.454 ± 0.228
0.363CysLeu: 0.363 ± 0.177
0.091CysMet: 0.091 ± 0.09
0.363CysAsn: 0.363 ± 0.19
0.363CysPro: 0.363 ± 0.177
0.363CysGln: 0.363 ± 0.227
0.635CysArg: 0.635 ± 0.265
0.544CysSer: 0.544 ± 0.201
0.635CysThr: 0.635 ± 0.23
0.454CysVal: 0.454 ± 0.196
0.0CysTrp: 0.0 ± 0.0
0.272CysTyr: 0.272 ± 0.147
0.0CysXaa: 0.0 ± 0.0
Asp
4.445AspAla: 4.445 ± 0.887
0.272AspCys: 0.272 ± 0.122
3.266AspAsp: 3.266 ± 0.636
4.626AspGlu: 4.626 ± 0.855
2.812AspPhe: 2.812 ± 0.647
3.901AspGly: 3.901 ± 0.522
0.998AspHis: 0.998 ± 0.271
5.715AspIle: 5.715 ± 0.947
4.263AspLys: 4.263 ± 0.55
6.531AspLeu: 6.531 ± 0.834
2.268AspMet: 2.268 ± 0.492
3.719AspAsn: 3.719 ± 0.441
2.177AspPro: 2.177 ± 0.417
1.179AspGln: 1.179 ± 0.375
2.449AspArg: 2.449 ± 0.514
4.626AspSer: 4.626 ± 0.617
3.628AspThr: 3.628 ± 0.656
4.626AspVal: 4.626 ± 0.529
0.635AspTrp: 0.635 ± 0.24
3.538AspTyr: 3.538 ± 0.458
0.0AspXaa: 0.0 ± 0.0
Glu
4.717GluAla: 4.717 ± 0.726
0.635GluCys: 0.635 ± 0.258
4.717GluAsp: 4.717 ± 0.697
6.259GluGlu: 6.259 ± 0.934
3.266GluPhe: 3.266 ± 0.467
4.717GluGly: 4.717 ± 0.787
0.726GluHis: 0.726 ± 0.261
4.354GluIle: 4.354 ± 0.554
5.171GluLys: 5.171 ± 1.034
6.531GluLeu: 6.531 ± 0.855
2.086GluMet: 2.086 ± 0.584
4.263GluAsn: 4.263 ± 0.591
2.086GluPro: 2.086 ± 0.547
3.175GluGln: 3.175 ± 0.411
2.721GluArg: 2.721 ± 0.602
3.084GluSer: 3.084 ± 0.554
3.447GluThr: 3.447 ± 0.546
4.898GluVal: 4.898 ± 0.713
0.816GluTrp: 0.816 ± 0.232
3.719GluTyr: 3.719 ± 0.598
0.0GluXaa: 0.0 ± 0.0
Phe
1.996PheAla: 1.996 ± 0.495
0.544PheCys: 0.544 ± 0.238
3.084PheAsp: 3.084 ± 0.446
3.901PheGlu: 3.901 ± 0.66
1.633PhePhe: 1.633 ± 0.406
3.175PheGly: 3.175 ± 0.695
0.726PheHis: 0.726 ± 0.206
3.266PheIle: 3.266 ± 0.557
3.538PheLys: 3.538 ± 0.782
3.538PheLeu: 3.538 ± 0.641
0.544PheMet: 0.544 ± 0.282
2.268PheAsn: 2.268 ± 0.379
1.27PhePro: 1.27 ± 0.302
1.905PheGln: 1.905 ± 0.379
1.179PheArg: 1.179 ± 0.303
2.177PheSer: 2.177 ± 0.377
2.631PheThr: 2.631 ± 0.503
2.721PheVal: 2.721 ± 0.529
0.272PheTrp: 0.272 ± 0.13
1.814PheTyr: 1.814 ± 0.542
0.0PheXaa: 0.0 ± 0.0
Gly
3.266GlyAla: 3.266 ± 0.878
0.363GlyCys: 0.363 ± 0.165
4.808GlyAsp: 4.808 ± 0.613
3.266GlyGlu: 3.266 ± 0.56
2.812GlyPhe: 2.812 ± 0.347
4.717GlyGly: 4.717 ± 0.924
1.179GlyHis: 1.179 ± 0.33
5.08GlyIle: 5.08 ± 0.749
5.261GlyLys: 5.261 ± 0.775
5.715GlyLeu: 5.715 ± 0.727
2.086GlyMet: 2.086 ± 0.721
3.356GlyAsn: 3.356 ± 0.46
0.0GlyPro: 0.0 ± 0.0
2.358GlyGln: 2.358 ± 0.447
2.812GlyArg: 2.812 ± 0.414
2.721GlySer: 2.721 ± 0.527
2.993GlyThr: 2.993 ± 0.466
4.808GlyVal: 4.808 ± 0.703
0.272GlyTrp: 0.272 ± 0.14
3.266GlyTyr: 3.266 ± 0.484
0.0GlyXaa: 0.0 ± 0.0
His
0.635HisAla: 0.635 ± 0.234
0.091HisCys: 0.091 ± 0.093
1.451HisAsp: 1.451 ± 0.378
0.907HisGlu: 0.907 ± 0.264
0.907HisPhe: 0.907 ± 0.247
1.361HisGly: 1.361 ± 0.309
0.816HisHis: 0.816 ± 0.395
0.998HisIle: 0.998 ± 0.293
0.726HisLys: 0.726 ± 0.275
2.086HisLeu: 2.086 ± 0.367
0.635HisMet: 0.635 ± 0.268
0.544HisAsn: 0.544 ± 0.201
0.544HisPro: 0.544 ± 0.214
0.635HisGln: 0.635 ± 0.264
0.454HisArg: 0.454 ± 0.183
1.179HisSer: 1.179 ± 0.443
0.816HisThr: 0.816 ± 0.281
1.361HisVal: 1.361 ± 0.325
0.091HisTrp: 0.091 ± 0.092
0.998HisTyr: 0.998 ± 0.315
0.0HisXaa: 0.0 ± 0.0
Ile
4.173IleAla: 4.173 ± 0.622
0.726IleCys: 0.726 ± 0.238
5.533IleAsp: 5.533 ± 0.668
4.808IleGlu: 4.808 ± 0.685
2.268IlePhe: 2.268 ± 0.428
3.356IleGly: 3.356 ± 0.742
0.635IleHis: 0.635 ± 0.225
5.261IleIle: 5.261 ± 0.573
7.257IleLys: 7.257 ± 0.7
5.987IleLeu: 5.987 ± 0.748
1.996IleMet: 1.996 ± 0.437
3.901IleAsn: 3.901 ± 0.634
3.084IlePro: 3.084 ± 0.598
2.812IleGln: 2.812 ± 0.569
4.263IleArg: 4.263 ± 0.645
4.354IleSer: 4.354 ± 0.581
3.628IleThr: 3.628 ± 0.635
5.261IleVal: 5.261 ± 0.665
0.998IleTrp: 0.998 ± 0.256
3.266IleTyr: 3.266 ± 0.55
0.0IleXaa: 0.0 ± 0.0
Lys
6.44LysAla: 6.44 ± 0.754
0.181LysCys: 0.181 ± 0.11
4.082LysAsp: 4.082 ± 0.648
5.443LysGlu: 5.443 ± 0.867
3.175LysPhe: 3.175 ± 0.674
6.622LysGly: 6.622 ± 1.105
1.905LysHis: 1.905 ± 0.396
6.35LysIle: 6.35 ± 0.765
5.533LysLys: 5.533 ± 0.929
6.078LysLeu: 6.078 ± 0.724
3.719LysMet: 3.719 ± 0.586
5.443LysAsn: 5.443 ± 0.837
2.268LysPro: 2.268 ± 0.695
2.631LysGln: 2.631 ± 0.523
3.084LysArg: 3.084 ± 0.555
2.993LysSer: 2.993 ± 0.505
4.808LysThr: 4.808 ± 0.522
6.35LysVal: 6.35 ± 0.818
0.726LysTrp: 0.726 ± 0.236
4.082LysTyr: 4.082 ± 0.638
0.0LysXaa: 0.0 ± 0.0
Leu
4.536LeuAla: 4.536 ± 0.729
0.726LeuCys: 0.726 ± 0.26
7.438LeuAsp: 7.438 ± 0.796
6.531LeuGlu: 6.531 ± 0.852
2.903LeuPhe: 2.903 ± 0.574
4.989LeuGly: 4.989 ± 0.641
1.542LeuHis: 1.542 ± 0.399
4.263LeuIle: 4.263 ± 0.742
7.438LeuLys: 7.438 ± 0.901
6.803LeuLeu: 6.803 ± 0.953
1.542LeuMet: 1.542 ± 0.367
5.715LeuAsn: 5.715 ± 0.66
2.993LeuPro: 2.993 ± 0.435
2.721LeuGln: 2.721 ± 0.506
4.082LeuArg: 4.082 ± 0.574
4.354LeuSer: 4.354 ± 0.493
6.078LeuThr: 6.078 ± 0.635
5.715LeuVal: 5.715 ± 0.618
1.089LeuTrp: 1.089 ± 0.343
2.993LeuTyr: 2.993 ± 0.507
0.0LeuXaa: 0.0 ± 0.0
Met
1.996MetAla: 1.996 ± 0.447
0.544MetCys: 0.544 ± 0.313
1.814MetAsp: 1.814 ± 0.465
1.814MetGlu: 1.814 ± 0.356
1.451MetPhe: 1.451 ± 0.307
1.724MetGly: 1.724 ± 0.562
0.363MetHis: 0.363 ± 0.191
1.542MetIle: 1.542 ± 0.356
2.358MetLys: 2.358 ± 0.384
2.177MetLeu: 2.177 ± 0.403
0.544MetMet: 0.544 ± 0.214
1.542MetAsn: 1.542 ± 0.412
1.089MetPro: 1.089 ± 0.319
0.907MetGln: 0.907 ± 0.303
1.542MetArg: 1.542 ± 0.421
1.724MetSer: 1.724 ± 0.319
2.268MetThr: 2.268 ± 0.425
1.542MetVal: 1.542 ± 0.33
0.454MetTrp: 0.454 ± 0.253
0.816MetTyr: 0.816 ± 0.248
0.0MetXaa: 0.0 ± 0.0
Asn
2.993AsnAla: 2.993 ± 0.527
0.726AsnCys: 0.726 ± 0.267
3.447AsnAsp: 3.447 ± 0.574
3.447AsnGlu: 3.447 ± 0.616
2.721AsnPhe: 2.721 ± 0.637
4.082AsnGly: 4.082 ± 0.728
0.998AsnHis: 0.998 ± 0.252
5.171AsnIle: 5.171 ± 0.684
4.989AsnLys: 4.989 ± 0.713
4.626AsnLeu: 4.626 ± 0.699
1.814AsnMet: 1.814 ± 0.375
3.447AsnAsn: 3.447 ± 0.62
2.721AsnPro: 2.721 ± 0.545
2.54AsnGln: 2.54 ± 0.451
2.631AsnArg: 2.631 ± 0.426
2.449AsnSer: 2.449 ± 0.538
3.538AsnThr: 3.538 ± 0.573
4.354AsnVal: 4.354 ± 0.576
0.544AsnTrp: 0.544 ± 0.214
1.814AsnTyr: 1.814 ± 0.35
0.0AsnXaa: 0.0 ± 0.0
Pro
2.993ProAla: 2.993 ± 0.567
0.272ProCys: 0.272 ± 0.154
2.177ProAsp: 2.177 ± 0.424
2.358ProGlu: 2.358 ± 0.502
1.451ProPhe: 1.451 ± 0.338
0.544ProGly: 0.544 ± 0.192
0.454ProHis: 0.454 ± 0.184
2.177ProIle: 2.177 ± 0.411
2.449ProLys: 2.449 ± 0.481
1.996ProLeu: 1.996 ± 0.504
0.635ProMet: 0.635 ± 0.219
2.268ProAsn: 2.268 ± 0.428
0.907ProPro: 0.907 ± 0.287
1.361ProGln: 1.361 ± 0.346
1.179ProArg: 1.179 ± 0.272
1.996ProSer: 1.996 ± 0.54
3.266ProThr: 3.266 ± 0.553
3.084ProVal: 3.084 ± 0.557
0.272ProTrp: 0.272 ± 0.145
1.542ProTyr: 1.542 ± 0.375
0.0ProXaa: 0.0 ± 0.0
Gln
2.54GlnAla: 2.54 ± 0.573
0.181GlnCys: 0.181 ± 0.14
1.542GlnAsp: 1.542 ± 0.311
3.538GlnGlu: 3.538 ± 0.604
1.089GlnPhe: 1.089 ± 0.316
2.086GlnGly: 2.086 ± 0.49
0.726GlnHis: 0.726 ± 0.222
2.358GlnIle: 2.358 ± 0.435
2.903GlnLys: 2.903 ± 0.596
3.084GlnLeu: 3.084 ± 0.475
0.454GlnMet: 0.454 ± 0.239
1.542GlnAsn: 1.542 ± 0.339
0.726GlnPro: 0.726 ± 0.19
0.907GlnGln: 0.907 ± 0.248
1.27GlnArg: 1.27 ± 0.351
2.54GlnSer: 2.54 ± 0.518
1.724GlnThr: 1.724 ± 0.402
3.266GlnVal: 3.266 ± 0.627
0.181GlnTrp: 0.181 ± 0.157
1.542GlnTyr: 1.542 ± 0.3
0.0GlnXaa: 0.0 ± 0.0
Arg
1.814ArgAla: 1.814 ± 0.388
0.272ArgCys: 0.272 ± 0.13
3.084ArgAsp: 3.084 ± 0.462
4.263ArgGlu: 4.263 ± 0.644
2.449ArgPhe: 2.449 ± 0.53
2.993ArgGly: 2.993 ± 0.501
1.27ArgHis: 1.27 ± 0.346
2.54ArgIle: 2.54 ± 0.554
3.447ArgLys: 3.447 ± 0.636
3.175ArgLeu: 3.175 ± 0.569
1.27ArgMet: 1.27 ± 0.37
1.996ArgAsn: 1.996 ± 0.489
1.089ArgPro: 1.089 ± 0.32
1.089ArgGln: 1.089 ± 0.294
1.814ArgArg: 1.814 ± 0.411
1.633ArgSer: 1.633 ± 0.486
2.268ArgThr: 2.268 ± 0.407
1.905ArgVal: 1.905 ± 0.311
0.454ArgTrp: 0.454 ± 0.193
2.358ArgTyr: 2.358 ± 0.446
0.0ArgXaa: 0.0 ± 0.0
Ser
3.084SerAla: 3.084 ± 0.556
0.272SerCys: 0.272 ± 0.133
3.628SerAsp: 3.628 ± 0.641
3.175SerGlu: 3.175 ± 0.525
2.177SerPhe: 2.177 ± 0.459
3.901SerGly: 3.901 ± 0.656
1.089SerHis: 1.089 ± 0.341
4.626SerIle: 4.626 ± 0.519
5.443SerLys: 5.443 ± 1.088
4.626SerLeu: 4.626 ± 0.718
1.361SerMet: 1.361 ± 0.282
3.356SerAsn: 3.356 ± 0.524
1.905SerPro: 1.905 ± 0.353
2.449SerGln: 2.449 ± 0.547
1.996SerArg: 1.996 ± 0.447
4.082SerSer: 4.082 ± 0.806
3.356SerThr: 3.356 ± 0.436
2.903SerVal: 2.903 ± 0.592
1.089SerTrp: 1.089 ± 0.325
1.996SerTyr: 1.996 ± 0.368
0.0SerXaa: 0.0 ± 0.0
Thr
2.358ThrAla: 2.358 ± 0.477
0.635ThrCys: 0.635 ± 0.215
3.901ThrAsp: 3.901 ± 0.515
3.447ThrGlu: 3.447 ± 0.506
2.993ThrPhe: 2.993 ± 0.432
3.628ThrGly: 3.628 ± 0.674
0.998ThrHis: 0.998 ± 0.281
5.08ThrIle: 5.08 ± 0.401
5.171ThrLys: 5.171 ± 0.699
6.803ThrLeu: 6.803 ± 0.907
1.633ThrMet: 1.633 ± 0.385
2.54ThrAsn: 2.54 ± 0.537
3.084ThrPro: 3.084 ± 0.592
1.905ThrGln: 1.905 ± 0.38
2.268ThrArg: 2.268 ± 0.52
4.263ThrSer: 4.263 ± 0.69
4.536ThrThr: 4.536 ± 0.575
4.898ThrVal: 4.898 ± 0.733
0.544ThrTrp: 0.544 ± 0.201
1.724ThrTyr: 1.724 ± 0.428
0.0ThrXaa: 0.0 ± 0.0
Val
3.719ValAla: 3.719 ± 0.62
0.181ValCys: 0.181 ± 0.112
4.717ValAsp: 4.717 ± 0.594
4.898ValGlu: 4.898 ± 0.76
2.812ValPhe: 2.812 ± 0.537
4.082ValGly: 4.082 ± 0.595
0.816ValHis: 0.816 ± 0.287
5.443ValIle: 5.443 ± 0.516
6.894ValLys: 6.894 ± 0.715
4.173ValLeu: 4.173 ± 0.63
1.542ValMet: 1.542 ± 0.377
4.626ValAsn: 4.626 ± 0.601
2.812ValPro: 2.812 ± 0.587
1.905ValGln: 1.905 ± 0.407
3.356ValArg: 3.356 ± 0.62
4.263ValSer: 4.263 ± 0.534
5.352ValThr: 5.352 ± 1.049
4.173ValVal: 4.173 ± 0.784
0.998ValTrp: 0.998 ± 0.348
2.721ValTyr: 2.721 ± 0.502
0.0ValXaa: 0.0 ± 0.0
Trp
0.454TrpAla: 0.454 ± 0.204
0.091TrpCys: 0.091 ± 0.083
0.272TrpAsp: 0.272 ± 0.162
0.726TrpGlu: 0.726 ± 0.21
0.272TrpPhe: 0.272 ± 0.138
0.544TrpGly: 0.544 ± 0.24
0.454TrpHis: 0.454 ± 0.214
0.363TrpIle: 0.363 ± 0.171
1.179TrpLys: 1.179 ± 0.391
1.089TrpLeu: 1.089 ± 0.545
0.272TrpMet: 0.272 ± 0.125
0.816TrpAsn: 0.816 ± 0.259
0.091TrpPro: 0.091 ± 0.091
0.635TrpGln: 0.635 ± 0.243
0.363TrpArg: 0.363 ± 0.176
0.998TrpSer: 0.998 ± 0.291
0.544TrpThr: 0.544 ± 0.25
0.907TrpVal: 0.907 ± 0.318
0.091TrpTrp: 0.091 ± 0.091
0.544TrpTyr: 0.544 ± 0.217
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.449TyrAla: 2.449 ± 0.453
0.544TyrCys: 0.544 ± 0.19
2.449TyrAsp: 2.449 ± 0.467
3.628TyrGlu: 3.628 ± 0.478
1.814TyrPhe: 1.814 ± 0.462
1.542TyrGly: 1.542 ± 0.48
0.635TyrHis: 0.635 ± 0.264
3.266TyrIle: 3.266 ± 0.502
2.54TyrLys: 2.54 ± 0.526
3.81TyrLeu: 3.81 ± 0.567
1.179TyrMet: 1.179 ± 0.308
2.993TyrAsn: 2.993 ± 0.528
1.542TyrPro: 1.542 ± 0.341
0.907TyrGln: 0.907 ± 0.321
1.814TyrArg: 1.814 ± 0.488
3.084TyrSer: 3.084 ± 0.56
3.628TyrThr: 3.628 ± 0.526
2.903TyrVal: 2.903 ± 0.502
0.726TyrTrp: 0.726 ± 0.194
2.812TyrTyr: 2.812 ± 0.524
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (11025 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski