Lactobacillus buchneri subsp. silagei CD034

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lentilactobacillus; Lentilactobacillus buchneri; Lentilactobacillus buchneri subsp. silagei

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2369 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9W2P5|J9W2P5_LACBU ABC-type multidrug transport system ATPase component OS=Lactobacillus buchneri subsp. silagei CD034 OX=1071400 GN=LBUCD034_0201 PE=4 SV=1
MM1 pKa = 7.16TVNPTEE7 pKa = 4.94LMDD10 pKa = 4.62EE11 pKa = 4.12LHH13 pKa = 6.94IDD15 pKa = 3.53QSPTEE20 pKa = 4.28LTTVTNLINEE30 pKa = 3.98ATEE33 pKa = 4.14IVNHH37 pKa = 5.9SVSSTEE43 pKa = 3.77TQYY46 pKa = 11.13QASSIYY52 pKa = 10.75DD53 pKa = 3.59LAIKK57 pKa = 9.14TLATQLYY64 pKa = 8.76YY65 pKa = 10.76DD66 pKa = 4.28RR67 pKa = 11.84EE68 pKa = 4.2LSKK71 pKa = 11.05GMSAGLLMMLDD82 pKa = 3.82QLQGMVSGSDD92 pKa = 3.45PDD94 pKa = 3.82GTT96 pKa = 4.04

Molecular weight: 10.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9W573|J9W573_LACBU Putative prephenate dehydrogenase OS=Lactobacillus buchneri subsp. silagei CD034 OX=1071400 GN=tyrA PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.19KK9 pKa = 7.37RR10 pKa = 11.84HH11 pKa = 5.5RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 5.84GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.01VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.57GRR39 pKa = 11.84KK40 pKa = 8.75SLSAA44 pKa = 3.86

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2369

0

2369

731389

39

2650

308.7

34.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.757 ± 0.051

0.43 ± 0.01

6.221 ± 0.05

4.934 ± 0.054

4.349 ± 0.036

6.844 ± 0.048

2.227 ± 0.021

7.147 ± 0.048

6.668 ± 0.048

9.23 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.709 ± 0.025

5.052 ± 0.044

3.812 ± 0.03

4.487 ± 0.038

3.878 ± 0.034

6.259 ± 0.041

6.197 ± 0.049

7.14 ± 0.039

1.036 ± 0.02

3.623 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski