Leek white stripe virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Betanecrovirus

Average proteome isoelectric point is 7.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q83106|Q83106_9TOMB Capsid protein OS=Leek white stripe virus OX=45224 GN=ORF 5 PE=3 SV=1
MM1 pKa = 7.14VIVVRR6 pKa = 11.84EE7 pKa = 3.95SSFPLLAVWIIVIVLVAVIGALLQTPPEE35 pKa = 4.51TINQVFNEE43 pKa = 4.27DD44 pKa = 3.24HH45 pKa = 7.25HH46 pKa = 5.86KK47 pKa = 8.4TQYY50 pKa = 11.06INIGKK55 pKa = 8.89PP56 pKa = 2.96

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q83103|Q83103_9TOMB RNA-directed RNA polymerase OS=Leek white stripe virus OX=45224 GN=ORF 2 PE=3 SV=1
MM1 pKa = 7.41HH2 pKa = 7.39TPQCIARR9 pKa = 11.84FGTTCHH15 pKa = 4.79THH17 pKa = 5.26IRR19 pKa = 11.84TARR22 pKa = 11.84LLYY25 pKa = 10.88VKK27 pKa = 7.83TTYY30 pKa = 9.7FQRR33 pKa = 11.84FLFMFFVYY41 pKa = 10.33SAVPVLFVPILFIAFFHH58 pKa = 7.09RR59 pKa = 11.84FFKK62 pKa = 10.83LQLGFSPEE70 pKa = 3.82ATALMHH76 pKa = 6.85RR77 pKa = 11.84FVDD80 pKa = 4.27LFSRR84 pKa = 11.84DD85 pKa = 3.91DD86 pKa = 3.7EE87 pKa = 4.36PVITEE92 pKa = 4.09TSCNVDD98 pKa = 4.14FLPGPEE104 pKa = 3.92SRR106 pKa = 11.84KK107 pKa = 9.98EE108 pKa = 3.68NLVAPKK114 pKa = 10.07RR115 pKa = 11.84RR116 pKa = 11.84NHH118 pKa = 6.37YY119 pKa = 8.86IVKK122 pKa = 9.89VAMAAKK128 pKa = 9.93SQVGLLGNSRR138 pKa = 11.84ANEE141 pKa = 3.65LVYY144 pKa = 10.97ARR146 pKa = 11.84LCRR149 pKa = 11.84EE150 pKa = 3.88EE151 pKa = 4.09MVKK154 pKa = 10.57HH155 pKa = 5.76GVRR158 pKa = 11.84PSHH161 pKa = 6.92IARR164 pKa = 11.84MVPLAVDD171 pKa = 3.6CHH173 pKa = 6.7CFIPMDD179 pKa = 4.4EE180 pKa = 5.71DD181 pKa = 3.35IMAAEE186 pKa = 4.35MMRR189 pKa = 11.84TDD191 pKa = 3.09VMRR194 pKa = 11.84SRR196 pKa = 11.84RR197 pKa = 11.84KK198 pKa = 10.73DD199 pKa = 3.16MGDD202 pKa = 3.06SHH204 pKa = 7.48AKK206 pKa = 9.59

Molecular weight: 23.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1340

56

727

268.0

30.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.567 ± 0.621

2.09 ± 0.348

4.328 ± 0.34

5.299 ± 0.72

6.791 ± 0.482

6.343 ± 1.034

3.06 ± 0.574

5.0 ± 0.917

5.149 ± 0.28

8.06 ± 0.997

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.731 ± 0.544

4.403 ± 1.32

5.373 ± 0.333

2.836 ± 0.282

6.94 ± 1.064

6.94 ± 1.206

5.597 ± 0.15

7.761 ± 0.894

0.896 ± 0.299

2.761 ± 0.343
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski