Mycobacterium phage NormanBulbieJr
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5HDU3|A0A2Z5HDU3_9CAUD Uncharacterized protein OS=Mycobacterium phage NormanBulbieJr OX=2250383 GN=61 PE=4 SV=1
MM1 pKa = 7.24 MSADD5 pKa = 3.89 PVRR8 pKa = 11.84 GAIQASLDD16 pKa = 3.5 AMGDD20 pKa = 3.44 GWQVAHH26 pKa = 5.73 YY27 pKa = 9.08 VVVVGLEE34 pKa = 4.17 RR35 pKa = 11.84 IDD37 pKa = 4.1 GDD39 pKa = 4.67 RR40 pKa = 11.84 MDD42 pKa = 5.5 LGATTVITPIGQAGYY57 pKa = 8.35 VTDD60 pKa = 4.2 GLVNRR65 pKa = 11.84 YY66 pKa = 7.35 WDD68 pKa = 3.73 EE69 pKa = 4.28 SSGEE73 pKa = 3.97
Molecular weight: 7.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.188
IPC2_protein 4.024
IPC_protein 3.935
Toseland 3.732
ProMoST 4.062
Dawson 3.948
Bjellqvist 4.202
Wikipedia 3.923
Rodwell 3.77
Grimsley 3.643
Solomon 3.923
Lehninger 3.884
Nozaki 4.075
DTASelect 4.329
Thurlkill 3.808
EMBOSS 3.923
Sillero 4.062
Patrickios 3.503
IPC_peptide 3.923
IPC2_peptide 4.037
IPC2.peptide.svr19 3.991
Protein with the highest isoelectric point:
>tr|A0A2Z5HDX9|A0A2Z5HDX9_9CAUD Uncharacterized protein OS=Mycobacterium phage NormanBulbieJr OX=2250383 GN=78 PE=4 SV=1
MM1 pKa = 6.36 TVIYY5 pKa = 10.3 IFIRR9 pKa = 11.84 TLFPILMIPYY19 pKa = 8.68 GQFAIRR25 pKa = 11.84 PTIEE29 pKa = 4.44 AGFSPWVVGTGVRR42 pKa = 11.84 SPSRR46 pKa = 11.84 GIPRR50 pKa = 11.84 RR51 pKa = 11.84 LHH53 pKa = 5.48 SRR55 pKa = 11.84 APATRR60 pKa = 11.84 KK61 pKa = 9.7 EE62 pKa = 4.07 PAVTLSVILAAQARR76 pKa = 11.84 FIHH79 pKa = 6.46 EE80 pKa = 4.39 SPVCPVCFQPRR91 pKa = 11.84 TEE93 pKa = 4.81 HH94 pKa = 6.19 STDD97 pKa = 3.26 CKK99 pKa = 10.54 GHH101 pKa = 6.13 HH102 pKa = 6.49 KK103 pKa = 10.63
Molecular weight: 11.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.604
IPC_protein 10.54
Toseland 10.613
ProMoST 10.511
Dawson 10.73
Bjellqvist 10.496
Wikipedia 10.965
Rodwell 10.833
Grimsley 10.789
Solomon 10.877
Lehninger 10.847
Nozaki 10.657
DTASelect 10.482
Thurlkill 10.628
EMBOSS 11.038
Sillero 10.672
Patrickios 10.643
IPC_peptide 10.877
IPC2_peptide 9.867
IPC2.peptide.svr19 8.53
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
102
0
102
18538
30
1175
181.7
19.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.508 ± 0.47
1.273 ± 0.177
6.511 ± 0.222
5.966 ± 0.277
2.891 ± 0.19
8.706 ± 0.533
2.374 ± 0.227
4.547 ± 0.213
3.404 ± 0.221
7.223 ± 0.196
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.282 ± 0.115
3.237 ± 0.138
6.101 ± 0.197
3.496 ± 0.19
6.964 ± 0.365
5.966 ± 0.342
6.673 ± 0.265
7.201 ± 0.284
2.384 ± 0.138
2.293 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here