Amino acid dipepetide frequency for Mycobacterium phage NormanBulbieJr

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.81AlaAla: 13.81 ± 1.548
1.187AlaCys: 1.187 ± 0.245
7.445AlaAsp: 7.445 ± 0.603
6.851AlaGlu: 6.851 ± 0.59
2.805AlaPhe: 2.805 ± 0.453
9.98AlaGly: 9.98 ± 1.244
2.535AlaHis: 2.535 ± 0.4
4.478AlaIle: 4.478 ± 0.476
4.424AlaLys: 4.424 ± 0.468
7.876AlaLeu: 7.876 ± 0.766
2.104AlaMet: 2.104 ± 0.32
2.643AlaAsn: 2.643 ± 0.394
4.747AlaPro: 4.747 ± 0.571
3.56AlaGln: 3.56 ± 0.508
7.445AlaArg: 7.445 ± 0.709
5.395AlaSer: 5.395 ± 0.643
6.743AlaThr: 6.743 ± 0.578
6.851AlaVal: 6.851 ± 0.719
2.212AlaTrp: 2.212 ± 0.368
1.996AlaTyr: 1.996 ± 0.321
0.0AlaXaa: 0.0 ± 0.0
Cys
0.917CysAla: 0.917 ± 0.236
0.0CysCys: 0.0 ± 0.0
1.241CysAsp: 1.241 ± 0.327
0.863CysGlu: 0.863 ± 0.221
0.162CysPhe: 0.162 ± 0.086
1.996CysGly: 1.996 ± 0.418
0.378CysHis: 0.378 ± 0.144
0.108CysIle: 0.108 ± 0.08
0.539CysLys: 0.539 ± 0.165
0.701CysLeu: 0.701 ± 0.232
0.162CysMet: 0.162 ± 0.09
0.432CysAsn: 0.432 ± 0.14
1.403CysPro: 1.403 ± 0.281
0.27CysGln: 0.27 ± 0.132
0.593CysArg: 0.593 ± 0.212
0.917CysSer: 0.917 ± 0.257
0.647CysThr: 0.647 ± 0.198
0.755CysVal: 0.755 ± 0.17
0.378CysTrp: 0.378 ± 0.127
0.27CysTyr: 0.27 ± 0.112
0.0CysXaa: 0.0 ± 0.0
Asp
6.635AspAla: 6.635 ± 0.515
0.863AspCys: 0.863 ± 0.188
4.154AspAsp: 4.154 ± 0.549
3.884AspGlu: 3.884 ± 0.507
1.726AspPhe: 1.726 ± 0.294
6.635AspGly: 6.635 ± 0.616
1.241AspHis: 1.241 ± 0.281
2.374AspIle: 2.374 ± 0.383
1.672AspLys: 1.672 ± 0.281
5.664AspLeu: 5.664 ± 0.476
1.025AspMet: 1.025 ± 0.252
1.78AspAsn: 1.78 ± 0.393
5.071AspPro: 5.071 ± 0.527
2.32AspGln: 2.32 ± 0.339
4.693AspArg: 4.693 ± 0.659
3.614AspSer: 3.614 ± 0.532
3.938AspThr: 3.938 ± 0.479
4.531AspVal: 4.531 ± 0.517
1.457AspTrp: 1.457 ± 0.268
1.834AspTyr: 1.834 ± 0.386
0.0AspXaa: 0.0 ± 0.0
Glu
6.312GluAla: 6.312 ± 0.779
1.241GluCys: 1.241 ± 0.377
3.183GluAsp: 3.183 ± 0.313
3.129GluGlu: 3.129 ± 0.598
2.05GluPhe: 2.05 ± 0.373
3.291GluGly: 3.291 ± 0.375
1.888GluHis: 1.888 ± 0.447
2.374GluIle: 2.374 ± 0.387
1.834GluLys: 1.834 ± 0.285
5.503GluLeu: 5.503 ± 0.698
1.618GluMet: 1.618 ± 0.309
1.618GluAsn: 1.618 ± 0.305
3.399GluPro: 3.399 ± 0.471
2.643GluGln: 2.643 ± 0.345
4.747GluArg: 4.747 ± 0.585
3.129GluSer: 3.129 ± 0.48
4.208GluThr: 4.208 ± 0.512
4.1GluVal: 4.1 ± 0.47
1.78GluTrp: 1.78 ± 0.301
1.564GluTyr: 1.564 ± 0.275
0.0GluXaa: 0.0 ± 0.0
Phe
2.859PheAla: 2.859 ± 0.355
0.216PheCys: 0.216 ± 0.104
2.643PheAsp: 2.643 ± 0.413
1.564PheGlu: 1.564 ± 0.293
0.917PhePhe: 0.917 ± 0.226
2.697PheGly: 2.697 ± 0.629
0.324PheHis: 0.324 ± 0.119
1.51PheIle: 1.51 ± 0.32
0.917PheLys: 0.917 ± 0.225
1.888PheLeu: 1.888 ± 0.313
0.701PheMet: 0.701 ± 0.183
1.187PheAsn: 1.187 ± 0.354
1.349PhePro: 1.349 ± 0.224
1.133PheGln: 1.133 ± 0.297
1.888PheArg: 1.888 ± 0.308
1.295PheSer: 1.295 ± 0.286
2.32PheThr: 2.32 ± 0.336
2.212PheVal: 2.212 ± 0.242
0.378PheTrp: 0.378 ± 0.129
0.917PheTyr: 0.917 ± 0.291
0.0PheXaa: 0.0 ± 0.0
Gly
8.416GlyAla: 8.416 ± 1.066
1.187GlyCys: 1.187 ± 0.298
5.287GlyAsp: 5.287 ± 0.469
5.125GlyGlu: 5.125 ± 0.603
2.428GlyPhe: 2.428 ± 0.355
9.98GlyGly: 9.98 ± 2.121
2.104GlyHis: 2.104 ± 0.325
3.938GlyIle: 3.938 ± 0.611
2.589GlyLys: 2.589 ± 0.34
5.556GlyLeu: 5.556 ± 0.549
2.589GlyMet: 2.589 ± 0.447
2.859GlyAsn: 2.859 ± 0.378
4.424GlyPro: 4.424 ± 0.589
2.428GlyGln: 2.428 ± 0.519
5.718GlyArg: 5.718 ± 0.595
6.15GlySer: 6.15 ± 0.936
6.15GlyThr: 6.15 ± 0.787
5.826GlyVal: 5.826 ± 0.527
2.482GlyTrp: 2.482 ± 0.36
1.834GlyTyr: 1.834 ± 0.354
0.0GlyXaa: 0.0 ± 0.0
His
2.266HisAla: 2.266 ± 0.384
0.432HisCys: 0.432 ± 0.175
1.241HisAsp: 1.241 ± 0.245
1.187HisGlu: 1.187 ± 0.312
0.539HisPhe: 0.539 ± 0.176
1.403HisGly: 1.403 ± 0.279
0.755HisHis: 0.755 ± 0.217
1.726HisIle: 1.726 ± 0.289
1.079HisLys: 1.079 ± 0.259
1.564HisLeu: 1.564 ± 0.327
0.539HisMet: 0.539 ± 0.128
0.971HisAsn: 0.971 ± 0.212
1.403HisPro: 1.403 ± 0.243
0.863HisGln: 0.863 ± 0.212
2.212HisArg: 2.212 ± 0.399
1.079HisSer: 1.079 ± 0.244
1.457HisThr: 1.457 ± 0.351
1.78HisVal: 1.78 ± 0.333
0.539HisTrp: 0.539 ± 0.179
0.701HisTyr: 0.701 ± 0.173
0.0HisXaa: 0.0 ± 0.0
Ile
5.61IleAla: 5.61 ± 0.583
0.701IleCys: 0.701 ± 0.245
3.776IleAsp: 3.776 ± 0.492
3.345IleGlu: 3.345 ± 0.478
0.809IlePhe: 0.809 ± 0.237
3.722IleGly: 3.722 ± 0.516
1.51IleHis: 1.51 ± 0.306
1.726IleIle: 1.726 ± 0.334
1.187IleLys: 1.187 ± 0.271
2.212IleLeu: 2.212 ± 0.354
0.432IleMet: 0.432 ± 0.13
1.618IleAsn: 1.618 ± 0.331
3.453IlePro: 3.453 ± 0.385
1.672IleGln: 1.672 ± 0.302
2.805IleArg: 2.805 ± 0.504
2.212IleSer: 2.212 ± 0.421
4.046IleThr: 4.046 ± 0.555
2.805IleVal: 2.805 ± 0.295
1.025IleTrp: 1.025 ± 0.232
0.809IleTyr: 0.809 ± 0.177
0.0IleXaa: 0.0 ± 0.0
Lys
3.776LysAla: 3.776 ± 0.521
0.593LysCys: 0.593 ± 0.153
1.672LysAsp: 1.672 ± 0.306
1.457LysGlu: 1.457 ± 0.246
1.241LysPhe: 1.241 ± 0.242
2.643LysGly: 2.643 ± 0.409
0.971LysHis: 0.971 ± 0.256
1.079LysIle: 1.079 ± 0.233
1.672LysLys: 1.672 ± 0.333
2.32LysLeu: 2.32 ± 0.5
0.809LysMet: 0.809 ± 0.14
1.187LysAsn: 1.187 ± 0.268
2.482LysPro: 2.482 ± 0.409
1.672LysGln: 1.672 ± 0.28
2.158LysArg: 2.158 ± 0.377
2.158LysSer: 2.158 ± 0.348
2.05LysThr: 2.05 ± 0.423
2.697LysVal: 2.697 ± 0.352
0.809LysTrp: 0.809 ± 0.243
0.593LysTyr: 0.593 ± 0.213
0.0LysXaa: 0.0 ± 0.0
Leu
8.038LeuAla: 8.038 ± 0.836
0.593LeuCys: 0.593 ± 0.176
4.531LeuAsp: 4.531 ± 0.598
4.262LeuGlu: 4.262 ± 0.541
2.428LeuPhe: 2.428 ± 0.291
4.801LeuGly: 4.801 ± 0.572
1.295LeuHis: 1.295 ± 0.26
2.913LeuIle: 2.913 ± 0.362
2.535LeuLys: 2.535 ± 0.468
5.287LeuLeu: 5.287 ± 0.628
1.78LeuMet: 1.78 ± 0.287
2.697LeuAsn: 2.697 ± 0.369
4.693LeuPro: 4.693 ± 0.696
2.859LeuGln: 2.859 ± 0.444
4.693LeuArg: 4.693 ± 0.619
5.341LeuSer: 5.341 ± 0.512
5.179LeuThr: 5.179 ± 0.44
4.909LeuVal: 4.909 ± 0.577
1.403LeuTrp: 1.403 ± 0.287
1.996LeuTyr: 1.996 ± 0.346
0.0LeuXaa: 0.0 ± 0.0
Met
2.212MetAla: 2.212 ± 0.362
0.162MetCys: 0.162 ± 0.129
1.457MetAsp: 1.457 ± 0.325
1.187MetGlu: 1.187 ± 0.212
0.539MetPhe: 0.539 ± 0.154
1.672MetGly: 1.672 ± 0.269
0.162MetHis: 0.162 ± 0.095
0.917MetIle: 0.917 ± 0.222
0.539MetLys: 0.539 ± 0.146
2.104MetLeu: 2.104 ± 0.33
0.432MetMet: 0.432 ± 0.208
0.809MetAsn: 0.809 ± 0.214
1.025MetPro: 1.025 ± 0.224
0.593MetGln: 0.593 ± 0.166
1.618MetArg: 1.618 ± 0.269
2.967MetSer: 2.967 ± 0.332
2.32MetThr: 2.32 ± 0.371
1.457MetVal: 1.457 ± 0.343
0.324MetTrp: 0.324 ± 0.127
0.324MetTyr: 0.324 ± 0.155
0.0MetXaa: 0.0 ± 0.0
Asn
3.237AsnAla: 3.237 ± 0.373
0.378AsnCys: 0.378 ± 0.161
1.672AsnAsp: 1.672 ± 0.296
1.51AsnGlu: 1.51 ± 0.333
0.755AsnPhe: 0.755 ± 0.222
3.938AsnGly: 3.938 ± 0.445
0.755AsnHis: 0.755 ± 0.179
1.51AsnIle: 1.51 ± 0.454
0.917AsnLys: 0.917 ± 0.201
2.266AsnLeu: 2.266 ± 0.361
1.025AsnMet: 1.025 ± 0.184
1.726AsnAsn: 1.726 ± 0.342
2.913AsnPro: 2.913 ± 0.32
1.079AsnGln: 1.079 ± 0.315
1.834AsnArg: 1.834 ± 0.358
1.403AsnSer: 1.403 ± 0.267
1.942AsnThr: 1.942 ± 0.321
2.104AsnVal: 2.104 ± 0.304
0.755AsnTrp: 0.755 ± 0.213
0.647AsnTyr: 0.647 ± 0.176
0.0AsnXaa: 0.0 ± 0.0
Pro
5.287ProAla: 5.287 ± 0.621
0.755ProCys: 0.755 ± 0.22
4.531ProAsp: 4.531 ± 0.519
4.208ProGlu: 4.208 ± 0.532
1.834ProPhe: 1.834 ± 0.314
6.743ProGly: 6.743 ± 0.69
1.564ProHis: 1.564 ± 0.253
1.942ProIle: 1.942 ± 0.349
2.158ProLys: 2.158 ± 0.367
4.747ProLeu: 4.747 ± 0.52
1.564ProMet: 1.564 ± 0.333
1.942ProAsn: 1.942 ± 0.267
3.938ProPro: 3.938 ± 0.648
2.266ProGln: 2.266 ± 0.416
3.345ProArg: 3.345 ± 0.474
3.938ProSer: 3.938 ± 0.531
3.614ProThr: 3.614 ± 0.492
4.154ProVal: 4.154 ± 0.445
1.133ProTrp: 1.133 ± 0.249
1.349ProTyr: 1.349 ± 0.298
0.0ProXaa: 0.0 ± 0.0
Gln
4.316GlnAla: 4.316 ± 0.564
0.27GlnCys: 0.27 ± 0.122
1.349GlnAsp: 1.349 ± 0.223
1.78GlnGlu: 1.78 ± 0.267
1.241GlnPhe: 1.241 ± 0.276
2.643GlnGly: 2.643 ± 0.472
1.187GlnHis: 1.187 ± 0.237
2.05GlnIle: 2.05 ± 0.33
1.403GlnLys: 1.403 ± 0.246
2.967GlnLeu: 2.967 ± 0.412
0.432GlnMet: 0.432 ± 0.154
1.025GlnAsn: 1.025 ± 0.226
2.482GlnPro: 2.482 ± 0.398
0.971GlnGln: 0.971 ± 0.266
2.535GlnArg: 2.535 ± 0.34
2.266GlnSer: 2.266 ± 0.316
1.78GlnThr: 1.78 ± 0.248
2.643GlnVal: 2.643 ± 0.372
0.755GlnTrp: 0.755 ± 0.209
0.863GlnTyr: 0.863 ± 0.2
0.0GlnXaa: 0.0 ± 0.0
Arg
7.337ArgAla: 7.337 ± 0.678
1.079ArgCys: 1.079 ± 0.312
4.801ArgAsp: 4.801 ± 0.57
4.855ArgGlu: 4.855 ± 0.607
2.212ArgPhe: 2.212 ± 0.378
3.884ArgGly: 3.884 ± 0.519
1.834ArgHis: 1.834 ± 0.538
4.046ArgIle: 4.046 ± 0.605
2.589ArgLys: 2.589 ± 0.397
4.909ArgLeu: 4.909 ± 0.579
2.212ArgMet: 2.212 ± 0.415
2.428ArgAsn: 2.428 ± 0.385
3.56ArgPro: 3.56 ± 0.387
1.888ArgGln: 1.888 ± 0.434
5.934ArgArg: 5.934 ± 0.726
3.614ArgSer: 3.614 ± 0.421
3.884ArgThr: 3.884 ± 0.532
4.585ArgVal: 4.585 ± 0.586
2.266ArgTrp: 2.266 ± 0.392
1.726ArgTyr: 1.726 ± 0.26
0.0ArgXaa: 0.0 ± 0.0
Ser
6.312SerAla: 6.312 ± 0.821
0.593SerCys: 0.593 ± 0.227
4.046SerAsp: 4.046 ± 0.586
3.129SerGlu: 3.129 ± 0.434
1.834SerPhe: 1.834 ± 0.282
5.826SerGly: 5.826 ± 0.969
1.349SerHis: 1.349 ± 0.209
2.967SerIle: 2.967 ± 0.415
2.104SerLys: 2.104 ± 0.358
3.453SerLeu: 3.453 ± 0.425
1.51SerMet: 1.51 ± 0.262
1.996SerAsn: 1.996 ± 0.375
3.668SerPro: 3.668 ± 0.35
1.996SerGln: 1.996 ± 0.284
3.83SerArg: 3.83 ± 0.4
3.776SerSer: 3.776 ± 0.697
3.668SerThr: 3.668 ± 0.502
5.125SerVal: 5.125 ± 0.646
1.025SerTrp: 1.025 ± 0.263
1.457SerTyr: 1.457 ± 0.229
0.0SerXaa: 0.0 ± 0.0
Thr
6.581ThrAla: 6.581 ± 0.567
0.647ThrCys: 0.647 ± 0.226
4.208ThrAsp: 4.208 ± 0.511
3.884ThrGlu: 3.884 ± 0.389
1.942ThrPhe: 1.942 ± 0.336
6.527ThrGly: 6.527 ± 0.62
1.834ThrHis: 1.834 ± 0.332
3.614ThrIle: 3.614 ± 0.473
1.942ThrLys: 1.942 ± 0.341
4.262ThrLeu: 4.262 ± 0.51
1.457ThrMet: 1.457 ± 0.282
1.996ThrAsn: 1.996 ± 0.343
4.424ThrPro: 4.424 ± 0.648
2.158ThrGln: 2.158 ± 0.314
4.37ThrArg: 4.37 ± 0.552
4.1ThrSer: 4.1 ± 0.553
4.424ThrThr: 4.424 ± 0.601
5.556ThrVal: 5.556 ± 0.645
1.241ThrTrp: 1.241 ± 0.339
1.564ThrTyr: 1.564 ± 0.333
0.0ThrXaa: 0.0 ± 0.0
Val
6.959ValAla: 6.959 ± 0.677
1.295ValCys: 1.295 ± 0.282
4.801ValAsp: 4.801 ± 0.519
4.478ValGlu: 4.478 ± 0.531
2.158ValPhe: 2.158 ± 0.328
5.61ValGly: 5.61 ± 0.661
1.403ValHis: 1.403 ± 0.306
3.776ValIle: 3.776 ± 0.441
2.374ValLys: 2.374 ± 0.416
5.61ValLeu: 5.61 ± 0.666
1.241ValMet: 1.241 ± 0.226
1.996ValAsn: 1.996 ± 0.366
3.668ValPro: 3.668 ± 0.396
2.751ValGln: 2.751 ± 0.356
5.125ValArg: 5.125 ± 0.711
4.424ValSer: 4.424 ± 0.648
4.855ValThr: 4.855 ± 0.543
6.042ValVal: 6.042 ± 0.686
2.212ValTrp: 2.212 ± 0.378
1.241ValTyr: 1.241 ± 0.27
0.0ValXaa: 0.0 ± 0.0
Trp
1.996TrpAla: 1.996 ± 0.375
0.27TrpCys: 0.27 ± 0.135
1.457TrpAsp: 1.457 ± 0.287
1.079TrpGlu: 1.079 ± 0.349
0.647TrpPhe: 0.647 ± 0.153
1.187TrpGly: 1.187 ± 0.262
0.486TrpHis: 0.486 ± 0.143
1.133TrpIle: 1.133 ± 0.206
0.809TrpLys: 0.809 ± 0.184
1.78TrpLeu: 1.78 ± 0.352
0.971TrpMet: 0.971 ± 0.224
0.701TrpAsn: 0.701 ± 0.246
1.295TrpPro: 1.295 ± 0.349
1.079TrpGln: 1.079 ± 0.237
2.482TrpArg: 2.482 ± 0.435
1.133TrpSer: 1.133 ± 0.245
1.996TrpThr: 1.996 ± 0.374
1.672TrpVal: 1.672 ± 0.426
1.025TrpTrp: 1.025 ± 0.215
0.647TrpTyr: 0.647 ± 0.197
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.212TyrAla: 2.212 ± 0.409
0.27TyrCys: 0.27 ± 0.123
1.618TyrAsp: 1.618 ± 0.398
1.888TyrGlu: 1.888 ± 0.329
0.647TyrPhe: 0.647 ± 0.188
1.888TyrGly: 1.888 ± 0.353
0.162TyrHis: 0.162 ± 0.085
1.295TyrIle: 1.295 ± 0.215
0.755TyrLys: 0.755 ± 0.264
1.564TyrLeu: 1.564 ± 0.292
0.162TyrMet: 0.162 ± 0.095
0.755TyrAsn: 0.755 ± 0.192
1.618TyrPro: 1.618 ± 0.301
0.755TyrGln: 0.755 ± 0.2
1.618TyrArg: 1.618 ± 0.328
0.755TyrSer: 0.755 ± 0.195
1.51TyrThr: 1.51 ± 0.263
2.212TyrVal: 2.212 ± 0.308
0.647TyrTrp: 0.647 ± 0.175
0.593TyrTyr: 0.593 ± 0.19
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 102 proteins (18538 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski