Klebsiella phage ST15-VIM1phi2.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482J2K8|A0A482J2K8_9CAUD Uncharacterized protein OS=Klebsiella phage ST15-VIM1phi2.1 OX=2555913 PE=4 SV=1
MM1 pKa = 7.39NNVDD5 pKa = 3.07WTIYY9 pKa = 8.99AQYY12 pKa = 11.17VNSTSLRR19 pKa = 11.84SLIDD23 pKa = 3.21TFNASVAPEE32 pKa = 3.82DD33 pKa = 4.41WIDD36 pKa = 3.37TFYY39 pKa = 11.7DD40 pKa = 3.51LVFNIEE46 pKa = 3.93TCGDD50 pKa = 3.75YY51 pKa = 11.65GLMCWGKK58 pKa = 9.3IVDD61 pKa = 3.96VEE63 pKa = 4.28RR64 pKa = 11.84LLTVTPSQQFLGFGEE79 pKa = 4.37ATSTPAEE86 pKa = 4.21LTDD89 pKa = 3.85PQPFNQAPFYY99 pKa = 10.9TGVQDD104 pKa = 3.58TNTVVLTNDD113 pKa = 3.87AYY115 pKa = 11.01RR116 pKa = 11.84KK117 pKa = 10.28LIMCKK122 pKa = 10.34AMANISDD129 pKa = 3.97CTVPVMNRR137 pKa = 11.84MLMYY141 pKa = 10.06MFGASGRR148 pKa = 11.84AYY150 pKa = 10.78VRR152 pKa = 11.84DD153 pKa = 4.24DD154 pKa = 3.84GNHH157 pKa = 4.05VMSYY161 pKa = 10.26VFEE164 pKa = 4.35FQLSEE169 pKa = 4.28SEE171 pKa = 4.11LAIVQSSGALPSPPGVKK188 pKa = 10.25VNIVQEE194 pKa = 4.31VV195 pKa = 3.07

Molecular weight: 21.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482J2X3|A0A482J2X3_9CAUD Uncharacterized protein OS=Klebsiella phage ST15-VIM1phi2.1 OX=2555913 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 8.66RR3 pKa = 11.84TPFYY7 pKa = 10.64RR8 pKa = 11.84RR9 pKa = 11.84PGKK12 pKa = 10.06AGKK15 pKa = 10.12FSGLRR20 pKa = 11.84EE21 pKa = 3.79RR22 pKa = 11.84VIWMIQTRR30 pKa = 11.84GRR32 pKa = 11.84PVTGSEE38 pKa = 3.59IAEE41 pKa = 4.27KK42 pKa = 10.62FGVTLVEE49 pKa = 4.59FNRR52 pKa = 11.84VANGITKK59 pKa = 10.54GEE61 pKa = 3.9GRR63 pKa = 11.84IAQLIASEE71 pKa = 4.18TWLNEE76 pKa = 4.2DD77 pKa = 4.91GICDD81 pKa = 3.48RR82 pKa = 11.84TFDD85 pKa = 3.9LVTRR89 pKa = 11.84PKK91 pKa = 10.82VITPQGKK98 pKa = 7.26TRR100 pKa = 11.84LFTKK104 pKa = 10.49RR105 pKa = 11.84SIAQAASGNRR115 pKa = 11.84QKK117 pKa = 11.15CIDD120 pKa = 3.14KK121 pKa = 9.97AARR124 pKa = 11.84RR125 pKa = 11.84RR126 pKa = 11.84RR127 pKa = 11.84LIASGLYY134 pKa = 8.29IDD136 pKa = 4.96EE137 pKa = 4.61MEE139 pKa = 4.47SVLL142 pKa = 5.09

Molecular weight: 16.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

14166

37

837

194.1

21.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.229 ± 0.443

1.052 ± 0.116

5.803 ± 0.262

6.099 ± 0.359

3.403 ± 0.164

7.532 ± 0.385

1.475 ± 0.158

5.605 ± 0.223

5.478 ± 0.295

7.716 ± 0.206

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.739 ± 0.129

4.299 ± 0.182

4.363 ± 0.213

4.123 ± 0.177

6.007 ± 0.461

6.876 ± 0.313

6.071 ± 0.295

6.339 ± 0.19

1.737 ± 0.137

3.057 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski