Streptococcus phage Javan565

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AN64|A0A4D6AN64_9CAUD Uncharacterized protein OS=Streptococcus phage Javan565 OX=2548260 GN=Javan565_0047 PE=4 SV=1
MM1 pKa = 7.27ITIQLDD7 pKa = 3.89EE8 pKa = 4.66EE9 pKa = 4.94LLTALIFAAAQSSCGFNQNTLQEE32 pKa = 4.08NQLWHH37 pKa = 6.24LHH39 pKa = 5.53CCDD42 pKa = 3.53YY43 pKa = 11.38NEE45 pKa = 4.47PVYY48 pKa = 10.62EE49 pKa = 4.08VAKK52 pKa = 10.53QINLDD57 pKa = 4.36DD58 pKa = 4.5IQDD61 pKa = 3.56EE62 pKa = 4.66SYY64 pKa = 10.49RR65 pKa = 11.84AYY67 pKa = 10.33FQEE70 pKa = 4.42VKK72 pKa = 10.88AKK74 pKa = 9.49GDD76 pKa = 3.18KK77 pKa = 10.48YY78 pKa = 10.29YY79 pKa = 11.36SEE81 pKa = 4.52VEE83 pKa = 4.26EE84 pKa = 4.24NEE86 pKa = 4.22KK87 pKa = 11.01

Molecular weight: 10.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ANC9|A0A4D6ANC9_9CAUD Holin OS=Streptococcus phage Javan565 OX=2548260 GN=Javan565_0003 PE=4 SV=1
MM1 pKa = 7.54RR2 pKa = 11.84PKK4 pKa = 10.31RR5 pKa = 11.84YY6 pKa = 9.01PYY8 pKa = 10.15SGKK11 pKa = 10.54LKK13 pKa = 10.49ASTMDD18 pKa = 3.65IVKK21 pKa = 9.87AWEE24 pKa = 4.05KK25 pKa = 10.52AYY27 pKa = 10.61SAYY30 pKa = 9.94RR31 pKa = 11.84VKK33 pKa = 10.64GQKK36 pKa = 9.34RR37 pKa = 11.84QEE39 pKa = 4.05KK40 pKa = 10.56AEE42 pKa = 3.99QEE44 pKa = 4.17LEE46 pKa = 4.05KK47 pKa = 10.41ATQRR51 pKa = 11.84LYY53 pKa = 11.07QLYY56 pKa = 9.79HH57 pKa = 6.12

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

11811

40

1290

181.7

20.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.197 ± 0.526

0.627 ± 0.11

6.189 ± 0.26

7.772 ± 0.425

4.293 ± 0.277

6.248 ± 0.365

1.541 ± 0.158

6.731 ± 0.218

8.009 ± 0.387

8.247 ± 0.315

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.506 ± 0.192

5.673 ± 0.224

2.574 ± 0.169

3.734 ± 0.253

4.496 ± 0.246

6.477 ± 0.354

6.079 ± 0.584

6.401 ± 0.274

1.236 ± 0.1

3.971 ± 0.288
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski