Flavobacteriaceae bacterium CWB-1

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Paucihalobacter

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3060 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A506PDI3|A0A506PDI3_9FLAO Response regulator transcription factor OS=Flavobacteriaceae bacterium CWB-1 OX=2567861 GN=FJ651_14300 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.17KK3 pKa = 10.63LSILGLFIFALISFNSCEE21 pKa = 4.27TEE23 pKa = 3.86DD24 pKa = 3.98DD25 pKa = 4.04VVFIANQADD34 pKa = 3.87FAFTNSFSAQYY45 pKa = 11.08VLTPIAAQNLGEE57 pKa = 4.16RR58 pKa = 11.84FTWNTPDD65 pKa = 3.23VGVPTNITYY74 pKa = 10.39QLQRR78 pKa = 11.84SIAGDD83 pKa = 3.4FSDD86 pKa = 4.33AVTVGATSSNEE97 pKa = 3.43IAITIGDD104 pKa = 4.11MLVIAAQAGLDD115 pKa = 3.54SDD117 pKa = 5.04PNTEE121 pKa = 4.21NPNTGQVSFRR131 pKa = 11.84VRR133 pKa = 11.84STVGDD138 pKa = 3.84GGLEE142 pKa = 4.33TYY144 pKa = 11.25SNTAVLTLVLPEE156 pKa = 4.56DD157 pKa = 3.97TTTAPAVCDD166 pKa = 3.58HH167 pKa = 7.07DD168 pKa = 4.16QLFLVGAGVKK178 pKa = 9.63FAGWSWDD185 pKa = 3.4TPQVINCSGNGVYY198 pKa = 9.58TGNVAFTNNVDD209 pKa = 3.04GDD211 pKa = 4.09GNFRR215 pKa = 11.84FFSTATDD222 pKa = 3.19WGSGTNFPGYY232 pKa = 10.94ANDD235 pKa = 4.95GYY237 pKa = 9.6TIDD240 pKa = 5.1SRR242 pKa = 11.84FEE244 pKa = 3.88DD245 pKa = 4.66AQDD248 pKa = 3.41GDD250 pKa = 4.06NNFLFTGASGLYY262 pKa = 10.0FLTIDD267 pKa = 5.48DD268 pKa = 3.9INKK271 pKa = 9.25TITLDD276 pKa = 3.78DD277 pKa = 3.94PTPTGSCDD285 pKa = 5.0LDD287 pKa = 3.6QLWAVGAGLPDD298 pKa = 5.13AGWSWDD304 pKa = 3.52TPIQFMCEE312 pKa = 3.98GNGVYY317 pKa = 9.97QGWVNLSPEE326 pKa = 3.71NDD328 pKa = 3.17GNFRR332 pKa = 11.84FFNTATDD339 pKa = 3.36WGSGTNYY346 pKa = 9.96PGFISQGYY354 pKa = 7.77TIDD357 pKa = 4.92NRR359 pKa = 11.84FQDD362 pKa = 4.58AQDD365 pKa = 3.56GDD367 pKa = 4.12NNFQFIGTAGQYY379 pKa = 9.36FLKK382 pKa = 10.13IDD384 pKa = 4.63TINKK388 pKa = 8.27TITLEE393 pKa = 3.96

Molecular weight: 42.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A506PLT4|A0A506PLT4_9FLAO Pyruvate kinase OS=Flavobacteriaceae bacterium CWB-1 OX=2567861 GN=pyk PE=3 SV=1
MM1 pKa = 7.45TFPTVKK7 pKa = 10.0NVKK10 pKa = 9.08VAGNRR15 pKa = 11.84RR16 pKa = 11.84SAQWPVRR23 pKa = 11.84VLFEE27 pKa = 4.9HH28 pKa = 6.05STSHH32 pKa = 6.9QLLCYY37 pKa = 10.03LDD39 pKa = 4.53SFEE42 pKa = 5.33LRR44 pKa = 11.84NPHH47 pKa = 5.89RR48 pKa = 11.84RR49 pKa = 11.84KK50 pKa = 9.41AAKK53 pKa = 9.58RR54 pKa = 11.84YY55 pKa = 9.74HH56 pKa = 6.26PFEE59 pKa = 5.3KK60 pKa = 10.72NILII64 pKa = 4.45

Molecular weight: 7.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3060

0

3060

1058090

38

3586

345.8

39.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.441 ± 0.047

0.751 ± 0.018

5.493 ± 0.034

6.276 ± 0.047

5.387 ± 0.039

6.282 ± 0.05

1.827 ± 0.025

8.043 ± 0.041

7.123 ± 0.072

9.474 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.141 ± 0.026

6.684 ± 0.067

3.461 ± 0.026

3.679 ± 0.025

3.388 ± 0.031

6.335 ± 0.036

5.874 ± 0.07

6.279 ± 0.037

1.063 ± 0.016

3.998 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski