Amino acid dipepetide frequency for Flavobacteriaceae bacterium CWB-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.492AlaAla: 4.492 ± 0.081	0.554AlaCys: 0.554 ± 0.024	3.373AlaAsp: 3.373 ± 0.068	4.007AlaGlu: 4.007 ± 0.075	3.478AlaPhe: 3.478 ± 0.063	4.181AlaGly: 4.181 ± 0.073	1.158AlaHis: 1.158 ± 0.037	5.472AlaIle: 5.472 ± 0.09	4.282AlaLys: 4.282 ± 0.086	6.413AlaLeu: 6.413 ± 0.091	1.56AlaMet: 1.56 ± 0.039	3.656AlaAsn: 3.656 ± 0.072	2.024AlaPro: 2.024 ± 0.054	2.642AlaGln: 2.642 ± 0.056	1.992AlaArg: 1.992 ± 0.05	3.801AlaSer: 3.801 ± 0.069	3.798AlaThr: 3.798 ± 0.105	4.463AlaVal: 4.463 ± 0.068	0.614AlaTrp: 0.614 ± 0.026	2.444AlaTyr: 2.444 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.495CysAla: 0.495 ± 0.028	0.098CysCys: 0.098 ± 0.011	0.482CysAsp: 0.482 ± 0.033	0.519CysGlu: 0.519 ± 0.026	0.448CysPhe: 0.448 ± 0.023	0.626CysGly: 0.626 ± 0.038	0.166CysHis: 0.166 ± 0.016	0.559CysIle: 0.559 ± 0.026	0.458CysLys: 0.458 ± 0.022	0.659CysLeu: 0.659 ± 0.024	0.129CysMet: 0.129 ± 0.012	0.438CysAsn: 0.438 ± 0.02	0.302CysPro: 0.302 ± 0.021	0.214CysGln: 0.214 ± 0.017	0.188CysArg: 0.188 ± 0.014	0.558CysSer: 0.558 ± 0.031	0.414CysThr: 0.414 ± 0.023	0.407CysVal: 0.407 ± 0.021	0.065CysTrp: 0.065 ± 0.008	0.282CysTyr: 0.282 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.769AspAla: 3.769 ± 0.062	0.431AspCys: 0.431 ± 0.027	3.065AspAsp: 3.065 ± 0.061	3.594AspGlu: 3.594 ± 0.063	3.549AspPhe: 3.549 ± 0.065	3.673AspGly: 3.673 ± 0.082	0.873AspHis: 0.873 ± 0.033	4.619AspIle: 4.619 ± 0.073	3.605AspLys: 3.605 ± 0.068	5.431AspLeu: 5.431 ± 0.079	1.114AspMet: 1.114 ± 0.033	3.464AspAsn: 3.464 ± 0.09	1.593AspPro: 1.593 ± 0.039	1.613AspGln: 1.613 ± 0.042	1.768AspArg: 1.768 ± 0.04	3.021AspSer: 3.021 ± 0.063	2.801AspThr: 2.801 ± 0.059	3.533AspVal: 3.533 ± 0.065	0.691AspTrp: 0.691 ± 0.023	2.728AspTyr: 2.728 ± 0.051	0.0AspXaa: 0.0 ± 0.0
Glu	4.53GluAla: 4.53 ± 0.08	0.384GluCys: 0.384 ± 0.037	3.529GluAsp: 3.529 ± 0.063	4.006GluGlu: 4.006 ± 0.08	3.057GluPhe: 3.057 ± 0.062	3.517GluGly: 3.517 ± 0.058	1.179GluHis: 1.179 ± 0.034	5.286GluIle: 5.286 ± 0.08	4.788GluLys: 4.788 ± 0.092	5.991GluLeu: 5.991 ± 0.083	1.472GluMet: 1.472 ± 0.047	4.347GluAsn: 4.347 ± 0.076	1.63GluPro: 1.63 ± 0.046	2.447GluGln: 2.447 ± 0.055	2.557GluArg: 2.557 ± 0.059	3.389GluSer: 3.389 ± 0.056	3.699GluThr: 3.699 ± 0.069	4.295GluVal: 4.295 ± 0.071	0.613GluTrp: 0.613 ± 0.026	2.039GluTyr: 2.039 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	2.958PheAla: 2.958 ± 0.058	0.4PheCys: 0.4 ± 0.019	3.316PheAsp: 3.316 ± 0.065	3.718PheGlu: 3.718 ± 0.07	2.786PhePhe: 2.786 ± 0.063	3.845PheGly: 3.845 ± 0.064	0.839PheHis: 0.839 ± 0.029	4.014PheIle: 4.014 ± 0.075	4.171PheLys: 4.171 ± 0.07	4.726PheLeu: 4.726 ± 0.091	1.11PheMet: 1.11 ± 0.038	3.956PheAsn: 3.956 ± 0.074	1.735PhePro: 1.735 ± 0.043	1.658PheGln: 1.658 ± 0.041	1.646PheArg: 1.646 ± 0.047	3.939PheSer: 3.939 ± 0.069	3.133PheThr: 3.133 ± 0.068	3.106PheVal: 3.106 ± 0.066	0.604PheTrp: 0.604 ± 0.026	2.211PheTyr: 2.211 ± 0.049	0.0PheXaa: 0.0 ± 0.0
Gly	4.125GlyAla: 4.125 ± 0.079	0.611GlyCys: 0.611 ± 0.035	3.302GlyAsp: 3.302 ± 0.06	3.469GlyGlu: 3.469 ± 0.064	3.749GlyPhe: 3.749 ± 0.068	4.432GlyGly: 4.432 ± 0.103	1.089GlyHis: 1.089 ± 0.036	5.358GlyIle: 5.358 ± 0.077	4.272GlyLys: 4.272 ± 0.079	5.897GlyLeu: 5.897 ± 0.091	1.556GlyMet: 1.556 ± 0.04	3.965GlyAsn: 3.965 ± 0.094	1.396GlyPro: 1.396 ± 0.043	1.918GlyGln: 1.918 ± 0.045	2.039GlyArg: 2.039 ± 0.052	3.893GlySer: 3.893 ± 0.085	4.037GlyThr: 4.037 ± 0.091	4.278GlyVal: 4.278 ± 0.072	0.762GlyTrp: 0.762 ± 0.036	2.671GlyTyr: 2.671 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	0.967HisAla: 0.967 ± 0.036	0.172HisCys: 0.172 ± 0.013	0.846HisAsp: 0.846 ± 0.028	1.005HisGlu: 1.005 ± 0.033	1.157HisPhe: 1.157 ± 0.035	1.044HisGly: 1.044 ± 0.031	0.558HisHis: 0.558 ± 0.024	1.561HisIle: 1.561 ± 0.04	1.178HisLys: 1.178 ± 0.035	1.895HisLeu: 1.895 ± 0.049	0.346HisMet: 0.346 ± 0.019	1.038HisAsn: 1.038 ± 0.031	0.925HisPro: 0.925 ± 0.035	0.856HisGln: 0.856 ± 0.036	0.646HisArg: 0.646 ± 0.021	1.046HisSer: 1.046 ± 0.036	0.916HisThr: 0.916 ± 0.027	0.977HisVal: 0.977 ± 0.03	0.21HisTrp: 0.21 ± 0.016	0.93HisTyr: 0.93 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.518IleAla: 5.518 ± 0.076	0.651IleCys: 0.651 ± 0.031	5.006IleAsp: 5.006 ± 0.073	5.566IleGlu: 5.566 ± 0.074	3.682IlePhe: 3.682 ± 0.065	5.066IleGly: 5.066 ± 0.085	1.332IleHis: 1.332 ± 0.038	6.276IleIle: 6.276 ± 0.108	5.964IleLys: 5.964 ± 0.094	7.215IleLeu: 7.215 ± 0.107	1.334IleMet: 1.334 ± 0.037	5.449IleAsn: 5.449 ± 0.082	3.295IlePro: 3.295 ± 0.057	2.489IleGln: 2.489 ± 0.05	2.388IleArg: 2.388 ± 0.054	5.655IleSer: 5.655 ± 0.077	5.138IleThr: 5.138 ± 0.089	4.831IleVal: 4.831 ± 0.082	0.762IleTrp: 0.762 ± 0.027	2.817IleTyr: 2.817 ± 0.048	0.0IleXaa: 0.0 ± 0.0
Lys	4.611LysAla: 4.611 ± 0.082	0.349LysCys: 0.349 ± 0.017	4.079LysAsp: 4.079 ± 0.064	4.81LysGlu: 4.81 ± 0.089	2.968LysPhe: 2.968 ± 0.061	3.883LysGly: 3.883 ± 0.069	1.486LysHis: 1.486 ± 0.04	5.692LysIle: 5.692 ± 0.089	5.236LysLys: 5.236 ± 0.083	6.529LysLeu: 6.529 ± 0.091	1.71LysMet: 1.71 ± 0.044	4.926LysAsn: 4.926 ± 0.074	2.46LysPro: 2.46 ± 0.053	2.989LysGln: 2.989 ± 0.063	2.79LysArg: 2.79 ± 0.056	4.467LysSer: 4.467 ± 0.08	4.44LysThr: 4.44 ± 0.079	4.343LysVal: 4.343 ± 0.072	0.7LysTrp: 0.7 ± 0.029	2.76LysTyr: 2.76 ± 0.062	0.0LysXaa: 0.0 ± 0.0
Leu	5.816LeuAla: 5.816 ± 0.084	0.665LeuCys: 0.665 ± 0.025	5.13LeuAsp: 5.13 ± 0.075	6.078LeuGlu: 6.078 ± 0.086	4.981LeuPhe: 4.981 ± 0.091	5.574LeuGly: 5.574 ± 0.101	1.578LeuHis: 1.578 ± 0.041	7.504LeuIle: 7.504 ± 0.109	7.813LeuLys: 7.813 ± 0.098	8.891LeuLeu: 8.891 ± 0.122	2.175LeuMet: 2.175 ± 0.053	6.535LeuAsn: 6.535 ± 0.092	3.713LeuPro: 3.713 ± 0.062	3.482LeuGln: 3.482 ± 0.062	3.172LeuArg: 3.172 ± 0.068	6.742LeuSer: 6.742 ± 0.089	5.248LeuThr: 5.248 ± 0.071	5.586LeuVal: 5.586 ± 0.078	0.892LeuTrp: 0.892 ± 0.035	3.167LeuTyr: 3.167 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	1.667MetAla: 1.667 ± 0.043	0.135MetCys: 0.135 ± 0.011	1.007MetAsp: 1.007 ± 0.034	1.226MetGlu: 1.226 ± 0.04	0.932MetPhe: 0.932 ± 0.032	1.299MetGly: 1.299 ± 0.038	0.401MetHis: 0.401 ± 0.019	1.598MetIle: 1.598 ± 0.038	1.886MetLys: 1.886 ± 0.045	2.038MetLeu: 2.038 ± 0.049	0.568MetMet: 0.568 ± 0.025	1.24MetAsn: 1.24 ± 0.033	0.912MetPro: 0.912 ± 0.03	0.814MetGln: 0.814 ± 0.03	0.871MetArg: 0.871 ± 0.032	1.455MetSer: 1.455 ± 0.043	1.171MetThr: 1.171 ± 0.033	1.367MetVal: 1.367 ± 0.043	0.164MetTrp: 0.164 ± 0.013	0.659MetTyr: 0.659 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	4.17AsnAla: 4.17 ± 0.068	0.586AsnCys: 0.586 ± 0.04	3.398AsnAsp: 3.398 ± 0.071	3.85AsnGlu: 3.85 ± 0.056	3.589AsnPhe: 3.589 ± 0.071	4.149AsnGly: 4.149 ± 0.1	1.212AsnHis: 1.212 ± 0.035	5.184AsnIle: 5.184 ± 0.084	4.176AsnLys: 4.176 ± 0.072	6.271AsnLeu: 6.271 ± 0.112	1.304AsnMet: 1.304 ± 0.04	5.126AsnAsn: 5.126 ± 0.126	2.99AsnPro: 2.99 ± 0.062	2.799AsnGln: 2.799 ± 0.052	2.362AsnArg: 2.362 ± 0.056	4.103AsnSer: 4.103 ± 0.076	3.885AsnThr: 3.885 ± 0.076	3.6AsnVal: 3.6 ± 0.073	0.852AsnTrp: 0.852 ± 0.034	3.236AsnTyr: 3.236 ± 0.072	0.0AsnXaa: 0.0 ± 0.0
Pro	2.035ProAla: 2.035 ± 0.058	0.218ProCys: 0.218 ± 0.015	2.172ProAsp: 2.172 ± 0.05	2.799ProGlu: 2.799 ± 0.061	1.947ProPhe: 1.947 ± 0.044	2.062ProGly: 2.062 ± 0.052	0.629ProHis: 0.629 ± 0.025	2.711ProIle: 2.711 ± 0.055	2.378ProLys: 2.378 ± 0.055	3.027ProLeu: 3.027 ± 0.054	0.695ProMet: 0.695 ± 0.027	2.53ProAsn: 2.53 ± 0.057	0.869ProPro: 0.869 ± 0.031	1.313ProGln: 1.313 ± 0.033	0.953ProArg: 0.953 ± 0.031	2.074ProSer: 2.074 ± 0.047	2.009ProThr: 2.009 ± 0.053	2.453ProVal: 2.453 ± 0.051	0.341ProTrp: 0.341 ± 0.018	1.396ProTyr: 1.396 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.194GlnAla: 2.194 ± 0.059	0.183GlnCys: 0.183 ± 0.015	1.754GlnAsp: 1.754 ± 0.047	2.102GlnGlu: 2.102 ± 0.052	1.92GlnPhe: 1.92 ± 0.043	1.863GlnGly: 1.863 ± 0.045	0.733GlnHis: 0.733 ± 0.025	3.055GlnIle: 3.055 ± 0.053	2.575GlnLys: 2.575 ± 0.046	4.115GlnLeu: 4.115 ± 0.076	0.837GlnMet: 0.837 ± 0.028	2.488GlnAsn: 2.488 ± 0.052	1.441GlnPro: 1.441 ± 0.04	1.816GlnGln: 1.816 ± 0.058	1.368GlnArg: 1.368 ± 0.036	2.212GlnSer: 2.212 ± 0.052	2.271GlnThr: 2.271 ± 0.048	2.027GlnVal: 2.027 ± 0.046	0.415GlnTrp: 0.415 ± 0.018	1.422GlnTyr: 1.422 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	2.144ArgAla: 2.144 ± 0.046	0.206ArgCys: 0.206 ± 0.016	1.846ArgAsp: 1.846 ± 0.043	2.057ArgGlu: 2.057 ± 0.057	1.984ArgPhe: 1.984 ± 0.054	1.906ArgGly: 1.906 ± 0.049	0.671ArgHis: 0.671 ± 0.028	2.873ArgIle: 2.873 ± 0.052	2.342ArgLys: 2.342 ± 0.054	3.262ArgLeu: 3.262 ± 0.055	0.844ArgMet: 0.844 ± 0.03	2.126ArgAsn: 2.126 ± 0.052	1.108ArgPro: 1.108 ± 0.032	1.312ArgGln: 1.312 ± 0.04	1.332ArgArg: 1.332 ± 0.045	1.996ArgSer: 1.996 ± 0.051	1.825ArgThr: 1.825 ± 0.044	2.05ArgVal: 2.05 ± 0.046	0.426ArgTrp: 0.426 ± 0.02	1.575ArgTyr: 1.575 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	3.848SerAla: 3.848 ± 0.056	0.577SerCys: 0.577 ± 0.024	3.241SerAsp: 3.241 ± 0.055	3.882SerGlu: 3.882 ± 0.064	3.816SerPhe: 3.816 ± 0.064	4.68SerGly: 4.68 ± 0.084	1.157SerHis: 1.157 ± 0.03	5.024SerIle: 5.024 ± 0.074	4.61SerLys: 4.61 ± 0.075	5.946SerLeu: 5.946 ± 0.083	1.183SerMet: 1.183 ± 0.032	4.265SerAsn: 4.265 ± 0.094	1.994SerPro: 1.994 ± 0.055	2.335SerGln: 2.335 ± 0.048	2.086SerArg: 2.086 ± 0.052	3.893SerSer: 3.893 ± 0.066	3.517SerThr: 3.517 ± 0.068	4.032SerVal: 4.032 ± 0.063	0.677SerTrp: 0.677 ± 0.026	2.584SerTyr: 2.584 ± 0.056	0.0SerXaa: 0.0 ± 0.0
Thr	3.983ThrAla: 3.983 ± 0.096	0.375ThrCys: 0.375 ± 0.026	3.234ThrAsp: 3.234 ± 0.076	3.415ThrGlu: 3.415 ± 0.061	3.155ThrPhe: 3.155 ± 0.073	4.162ThrGly: 4.162 ± 0.086	1.026ThrHis: 1.026 ± 0.032	4.946ThrIle: 4.946 ± 0.082	3.569ThrLys: 3.569 ± 0.055	5.37ThrLeu: 5.37 ± 0.071	0.993ThrMet: 0.993 ± 0.032	3.8ThrAsn: 3.8 ± 0.071	2.404ThrPro: 2.404 ± 0.052	2.028ThrGln: 2.028 ± 0.048	1.636ThrArg: 1.636 ± 0.047	3.761ThrSer: 3.761 ± 0.075	3.693ThrThr: 3.693 ± 0.092	4.115ThrVal: 4.115 ± 0.113	0.609ThrTrp: 0.609 ± 0.034	2.468ThrTyr: 2.468 ± 0.058	0.0ThrXaa: 0.0 ± 0.0
Val	4.091ValAla: 4.091 ± 0.071	0.487ValCys: 0.487 ± 0.021	3.486ValAsp: 3.486 ± 0.057	3.972ValGlu: 3.972 ± 0.065	3.507ValPhe: 3.507 ± 0.067	3.746ValGly: 3.746 ± 0.065	0.986ValHis: 0.986 ± 0.034	5.141ValIle: 5.141 ± 0.086	4.324ValLys: 4.324 ± 0.072	6.165ValLeu: 6.165 ± 0.09	1.418ValMet: 1.418 ± 0.042	3.856ValAsn: 3.856 ± 0.07	2.119ValPro: 2.119 ± 0.048	1.845ValGln: 1.845 ± 0.045	2.038ValArg: 2.038 ± 0.047	4.287ValSer: 4.287 ± 0.071	3.815ValThr: 3.815 ± 0.091	4.519ValVal: 4.519 ± 0.078	0.608ValTrp: 0.608 ± 0.026	2.375ValTyr: 2.375 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	0.58TrpAla: 0.58 ± 0.025	0.095TrpCys: 0.095 ± 0.009	0.559TrpAsp: 0.559 ± 0.026	0.607TrpGlu: 0.607 ± 0.026	0.647TrpPhe: 0.647 ± 0.033	0.608TrpGly: 0.608 ± 0.031	0.273TrpHis: 0.273 ± 0.017	0.763TrpIle: 0.763 ± 0.026	0.683TrpLys: 0.683 ± 0.027	1.077TrpLeu: 1.077 ± 0.038	0.318TrpMet: 0.318 ± 0.019	0.753TrpAsn: 0.753 ± 0.033	0.265TrpPro: 0.265 ± 0.018	0.486TrpGln: 0.486 ± 0.026	0.431TrpArg: 0.431 ± 0.021	0.64TrpSer: 0.64 ± 0.029	0.589TrpThr: 0.589 ± 0.032	0.664TrpVal: 0.664 ± 0.027	0.145TrpTrp: 0.145 ± 0.012	0.45TrpTyr: 0.45 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.411TyrAla: 2.411 ± 0.053	0.329TyrCys: 0.329 ± 0.017	2.109TyrAsp: 2.109 ± 0.053	2.074TyrGlu: 2.074 ± 0.044	2.518TyrPhe: 2.518 ± 0.053	2.504TyrGly: 2.504 ± 0.056	0.925TyrHis: 0.925 ± 0.032	2.797TyrIle: 2.797 ± 0.047	2.918TyrLys: 2.918 ± 0.061	3.828TyrLeu: 3.828 ± 0.066	0.744TyrMet: 0.744 ± 0.026	2.884TyrAsn: 2.884 ± 0.068	1.436TyrPro: 1.436 ± 0.036	1.737TyrGln: 1.737 ± 0.047	1.622TyrArg: 1.622 ± 0.041	2.413TyrSer: 2.413 ± 0.054	2.342TyrThr: 2.342 ± 0.054	2.139TyrVal: 2.139 ± 0.045	0.484TyrTrp: 0.484 ± 0.023	1.771TyrTyr: 1.771 ± 0.05	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3060 proteins (1058090 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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