Mycobacterium phage Strokeseat

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VFN9|A0A649VFN9_9CAUD Uncharacterized protein OS=Mycobacterium phage Strokeseat OX=2656607 GN=90 PE=4 SV=1
MM1 pKa = 7.36VSPASPLPAQRR12 pKa = 11.84VEE14 pKa = 4.21VTAVTGICICTHH26 pKa = 5.57YY27 pKa = 9.79RR28 pKa = 11.84TEE30 pKa = 4.16HH31 pKa = 6.94DD32 pKa = 3.22EE33 pKa = 4.48HH34 pKa = 7.05GNCLASDD41 pKa = 4.55LFGEE45 pKa = 4.76PCDD48 pKa = 4.17CPGYY52 pKa = 10.79EE53 pKa = 4.38PDD55 pKa = 4.48EE56 pKa = 4.82EE57 pKa = 4.78EE58 pKa = 5.62DD59 pKa = 4.83DD60 pKa = 3.96EE61 pKa = 4.68TT62 pKa = 5.08

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VG53|A0A649VG53_9CAUD Uncharacterized protein OS=Mycobacterium phage Strokeseat OX=2656607 GN=85 PE=4 SV=1
MM1 pKa = 6.68TQRR4 pKa = 11.84KK5 pKa = 9.5GGFDD9 pKa = 4.35WIRR12 pKa = 11.84STYY15 pKa = 9.77RR16 pKa = 11.84VPAKK20 pKa = 10.02RR21 pKa = 11.84GMRR24 pKa = 11.84VVFDD28 pKa = 3.72GRR30 pKa = 11.84PGRR33 pKa = 11.84ILSVDD38 pKa = 3.4GPYY41 pKa = 11.37LMLHH45 pKa = 6.91LDD47 pKa = 3.96SDD49 pKa = 4.41PKK51 pKa = 10.37NLRR54 pKa = 11.84TRR56 pKa = 11.84VHH58 pKa = 5.46PTWRR62 pKa = 11.84MEE64 pKa = 3.91YY65 pKa = 10.61LPP67 pKa = 4.85

Molecular weight: 7.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

101

0

101

17770

30

1211

175.9

19.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.321 ± 0.465

1.216 ± 0.164

6.747 ± 0.214

5.959 ± 0.315

3.028 ± 0.19

9.015 ± 0.615

2.319 ± 0.198

4.361 ± 0.208

3.213 ± 0.171

7.214 ± 0.244

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.114

3.444 ± 0.171

5.976 ± 0.19

3.41 ± 0.196

6.86 ± 0.433

5.954 ± 0.259

6.646 ± 0.264

7.293 ± 0.258

2.268 ± 0.157

2.482 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski