Amino acid dipepetide frequency for Mycobacterium phage Strokeseat

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.225AlaAla: 13.225 ± 1.752
0.732AlaCys: 0.732 ± 0.172
6.866AlaAsp: 6.866 ± 0.563
6.81AlaGlu: 6.81 ± 0.642
2.476AlaPhe: 2.476 ± 0.354
9.68AlaGly: 9.68 ± 1.633
2.814AlaHis: 2.814 ± 0.456
4.39AlaIle: 4.39 ± 0.68
3.883AlaLys: 3.883 ± 0.407
8.273AlaLeu: 8.273 ± 0.81
2.589AlaMet: 2.589 ± 0.392
2.645AlaAsn: 2.645 ± 0.323
4.952AlaPro: 4.952 ± 0.573
3.714AlaGln: 3.714 ± 0.447
6.922AlaArg: 6.922 ± 0.644
5.346AlaSer: 5.346 ± 0.656
6.472AlaThr: 6.472 ± 0.563
6.866AlaVal: 6.866 ± 0.629
2.476AlaTrp: 2.476 ± 0.369
2.082AlaTyr: 2.082 ± 0.319
0.0AlaXaa: 0.0 ± 0.0
Cys
0.788CysAla: 0.788 ± 0.196
0.113CysCys: 0.113 ± 0.084
1.463CysAsp: 1.463 ± 0.385
0.732CysGlu: 0.732 ± 0.205
0.225CysPhe: 0.225 ± 0.122
1.238CysGly: 1.238 ± 0.277
0.338CysHis: 0.338 ± 0.148
0.225CysIle: 0.225 ± 0.114
0.507CysLys: 0.507 ± 0.164
0.788CysLeu: 0.788 ± 0.258
0.169CysMet: 0.169 ± 0.096
0.394CysAsn: 0.394 ± 0.151
1.294CysPro: 1.294 ± 0.285
0.45CysGln: 0.45 ± 0.168
0.732CysArg: 0.732 ± 0.192
0.675CysSer: 0.675 ± 0.245
0.675CysThr: 0.675 ± 0.201
0.9CysVal: 0.9 ± 0.221
0.338CysTrp: 0.338 ± 0.144
0.113CysTyr: 0.113 ± 0.081
0.0CysXaa: 0.0 ± 0.0
Asp
7.316AspAla: 7.316 ± 0.616
0.844AspCys: 0.844 ± 0.202
4.502AspAsp: 4.502 ± 0.609
3.377AspGlu: 3.377 ± 0.492
1.97AspPhe: 1.97 ± 0.268
7.035AspGly: 7.035 ± 0.727
1.407AspHis: 1.407 ± 0.225
2.645AspIle: 2.645 ± 0.403
1.857AspLys: 1.857 ± 0.328
5.628AspLeu: 5.628 ± 0.49
1.069AspMet: 1.069 ± 0.297
1.745AspAsn: 1.745 ± 0.342
5.121AspPro: 5.121 ± 0.638
2.364AspGln: 2.364 ± 0.334
4.952AspArg: 4.952 ± 0.586
3.771AspSer: 3.771 ± 0.578
4.108AspThr: 4.108 ± 0.452
4.277AspVal: 4.277 ± 0.528
1.632AspTrp: 1.632 ± 0.342
1.857AspTyr: 1.857 ± 0.325
0.0AspXaa: 0.0 ± 0.0
Glu
5.628GluAla: 5.628 ± 0.608
1.013GluCys: 1.013 ± 0.262
3.095GluAsp: 3.095 ± 0.394
3.152GluGlu: 3.152 ± 0.519
2.251GluPhe: 2.251 ± 0.31
3.377GluGly: 3.377 ± 0.397
1.801GluHis: 1.801 ± 0.444
2.533GluIle: 2.533 ± 0.4
1.913GluLys: 1.913 ± 0.297
5.121GluLeu: 5.121 ± 0.603
1.688GluMet: 1.688 ± 0.298
2.307GluAsn: 2.307 ± 0.295
2.758GluPro: 2.758 ± 0.491
2.701GluGln: 2.701 ± 0.42
5.009GluArg: 5.009 ± 0.649
3.208GluSer: 3.208 ± 0.556
4.502GluThr: 4.502 ± 0.638
4.333GluVal: 4.333 ± 0.434
1.294GluTrp: 1.294 ± 0.294
1.913GluTyr: 1.913 ± 0.346
0.0GluXaa: 0.0 ± 0.0
Phe
3.32PheAla: 3.32 ± 0.406
0.225PheCys: 0.225 ± 0.103
2.87PheAsp: 2.87 ± 0.391
1.801PheGlu: 1.801 ± 0.337
0.844PhePhe: 0.844 ± 0.277
3.264PheGly: 3.264 ± 0.568
0.394PheHis: 0.394 ± 0.171
1.351PheIle: 1.351 ± 0.334
0.9PheLys: 0.9 ± 0.235
1.801PheLeu: 1.801 ± 0.276
0.9PheMet: 0.9 ± 0.261
1.182PheAsn: 1.182 ± 0.316
1.463PhePro: 1.463 ± 0.277
1.126PheGln: 1.126 ± 0.336
1.407PheArg: 1.407 ± 0.263
1.52PheSer: 1.52 ± 0.305
2.139PheThr: 2.139 ± 0.4
2.082PheVal: 2.082 ± 0.286
0.788PheTrp: 0.788 ± 0.218
0.9PheTyr: 0.9 ± 0.268
0.0PheXaa: 0.0 ± 0.0
Gly
9.286GlyAla: 9.286 ± 1.587
1.013GlyCys: 1.013 ± 0.254
6.134GlyAsp: 6.134 ± 0.638
4.333GlyGlu: 4.333 ± 0.554
2.758GlyPhe: 2.758 ± 0.439
11.312GlyGly: 11.312 ± 2.796
1.857GlyHis: 1.857 ± 0.262
4.165GlyIle: 4.165 ± 0.528
2.476GlyLys: 2.476 ± 0.342
6.022GlyLeu: 6.022 ± 0.582
2.251GlyMet: 2.251 ± 0.4
3.377GlyAsn: 3.377 ± 0.509
4.221GlyPro: 4.221 ± 0.665
2.195GlyGln: 2.195 ± 0.591
5.234GlyArg: 5.234 ± 0.562
6.359GlySer: 6.359 ± 0.874
6.078GlyThr: 6.078 ± 0.722
6.303GlyVal: 6.303 ± 0.621
2.307GlyTrp: 2.307 ± 0.396
2.476GlyTyr: 2.476 ± 0.379
0.0GlyXaa: 0.0 ± 0.0
His
2.026HisAla: 2.026 ± 0.381
0.45HisCys: 0.45 ± 0.17
1.069HisAsp: 1.069 ± 0.241
1.52HisGlu: 1.52 ± 0.32
0.507HisPhe: 0.507 ± 0.138
2.195HisGly: 2.195 ± 0.314
1.013HisHis: 1.013 ± 0.288
1.294HisIle: 1.294 ± 0.3
0.732HisLys: 0.732 ± 0.224
1.294HisLeu: 1.294 ± 0.285
0.507HisMet: 0.507 ± 0.151
0.675HisAsn: 0.675 ± 0.162
1.52HisPro: 1.52 ± 0.3
1.013HisGln: 1.013 ± 0.232
2.195HisArg: 2.195 ± 0.439
1.013HisSer: 1.013 ± 0.209
1.238HisThr: 1.238 ± 0.283
1.632HisVal: 1.632 ± 0.302
0.45HisTrp: 0.45 ± 0.178
0.844HisTyr: 0.844 ± 0.224
0.0HisXaa: 0.0 ± 0.0
Ile
4.39IleAla: 4.39 ± 0.54
0.9IleCys: 0.9 ± 0.308
3.827IleAsp: 3.827 ± 0.523
3.714IleGlu: 3.714 ± 0.37
1.126IlePhe: 1.126 ± 0.245
3.883IleGly: 3.883 ± 0.485
1.294IleHis: 1.294 ± 0.271
1.294IleIle: 1.294 ± 0.288
1.126IleLys: 1.126 ± 0.239
2.251IleLeu: 2.251 ± 0.375
0.563IleMet: 0.563 ± 0.166
1.576IleAsn: 1.576 ± 0.297
3.095IlePro: 3.095 ± 0.361
1.294IleGln: 1.294 ± 0.249
2.42IleArg: 2.42 ± 0.443
2.251IleSer: 2.251 ± 0.429
3.827IleThr: 3.827 ± 0.37
2.983IleVal: 2.983 ± 0.353
0.957IleTrp: 0.957 ± 0.219
0.844IleTyr: 0.844 ± 0.231
0.0IleXaa: 0.0 ± 0.0
Lys
3.377LysAla: 3.377 ± 0.41
0.394LysCys: 0.394 ± 0.141
1.632LysAsp: 1.632 ± 0.253
1.294LysGlu: 1.294 ± 0.238
1.294LysPhe: 1.294 ± 0.174
2.589LysGly: 2.589 ± 0.326
1.126LysHis: 1.126 ± 0.306
0.9LysIle: 0.9 ± 0.2
1.463LysLys: 1.463 ± 0.38
2.364LysLeu: 2.364 ± 0.387
0.788LysMet: 0.788 ± 0.184
0.9LysAsn: 0.9 ± 0.227
2.026LysPro: 2.026 ± 0.281
1.463LysGln: 1.463 ± 0.241
2.082LysArg: 2.082 ± 0.318
2.139LysSer: 2.139 ± 0.316
2.195LysThr: 2.195 ± 0.37
2.139LysVal: 2.139 ± 0.383
0.788LysTrp: 0.788 ± 0.225
1.182LysTyr: 1.182 ± 0.275
0.0LysXaa: 0.0 ± 0.0
Leu
6.697LeuAla: 6.697 ± 0.766
0.732LeuCys: 0.732 ± 0.216
4.84LeuAsp: 4.84 ± 0.496
4.052LeuGlu: 4.052 ± 0.475
2.645LeuPhe: 2.645 ± 0.342
5.684LeuGly: 5.684 ± 0.494
0.844LeuHis: 0.844 ± 0.203
3.602LeuIle: 3.602 ± 0.492
1.857LeuLys: 1.857 ± 0.301
4.446LeuLeu: 4.446 ± 0.401
1.632LeuMet: 1.632 ± 0.316
3.095LeuAsn: 3.095 ± 0.393
5.234LeuPro: 5.234 ± 0.567
3.095LeuGln: 3.095 ± 0.411
5.065LeuArg: 5.065 ± 0.713
5.065LeuSer: 5.065 ± 0.603
5.628LeuThr: 5.628 ± 0.54
4.671LeuVal: 4.671 ± 0.549
1.294LeuTrp: 1.294 ± 0.251
1.97LeuTyr: 1.97 ± 0.373
0.0LeuXaa: 0.0 ± 0.0
Met
2.364MetAla: 2.364 ± 0.354
0.056MetCys: 0.056 ± 0.056
1.351MetAsp: 1.351 ± 0.26
1.407MetGlu: 1.407 ± 0.252
0.675MetPhe: 0.675 ± 0.217
1.688MetGly: 1.688 ± 0.315
0.281MetHis: 0.281 ± 0.135
0.9MetIle: 0.9 ± 0.237
0.788MetLys: 0.788 ± 0.217
2.082MetLeu: 2.082 ± 0.31
0.45MetMet: 0.45 ± 0.229
1.013MetAsn: 1.013 ± 0.24
1.351MetPro: 1.351 ± 0.262
0.45MetGln: 0.45 ± 0.137
1.069MetArg: 1.069 ± 0.209
2.645MetSer: 2.645 ± 0.319
2.364MetThr: 2.364 ± 0.329
1.238MetVal: 1.238 ± 0.34
0.338MetTrp: 0.338 ± 0.138
0.225MetTyr: 0.225 ± 0.113
0.0MetXaa: 0.0 ± 0.0
Asn
3.658AsnAla: 3.658 ± 0.391
0.281AsnCys: 0.281 ± 0.128
1.913AsnAsp: 1.913 ± 0.317
1.801AsnGlu: 1.801 ± 0.355
0.732AsnPhe: 0.732 ± 0.288
3.996AsnGly: 3.996 ± 0.677
0.844AsnHis: 0.844 ± 0.183
1.576AsnIle: 1.576 ± 0.375
1.182AsnLys: 1.182 ± 0.298
2.364AsnLeu: 2.364 ± 0.373
0.675AsnMet: 0.675 ± 0.149
1.913AsnAsn: 1.913 ± 0.393
2.814AsnPro: 2.814 ± 0.289
1.126AsnGln: 1.126 ± 0.322
2.251AsnArg: 2.251 ± 0.428
1.576AsnSer: 1.576 ± 0.296
2.082AsnThr: 2.082 ± 0.322
1.913AsnVal: 1.913 ± 0.345
0.9AsnTrp: 0.9 ± 0.179
0.844AsnTyr: 0.844 ± 0.173
0.0AsnXaa: 0.0 ± 0.0
Pro
5.684ProAla: 5.684 ± 0.552
0.675ProCys: 0.675 ± 0.21
4.559ProAsp: 4.559 ± 0.551
4.108ProGlu: 4.108 ± 0.464
1.576ProPhe: 1.576 ± 0.324
6.359ProGly: 6.359 ± 0.568
1.52ProHis: 1.52 ± 0.299
2.026ProIle: 2.026 ± 0.346
1.913ProLys: 1.913 ± 0.374
4.502ProLeu: 4.502 ± 0.546
1.407ProMet: 1.407 ± 0.298
2.364ProAsn: 2.364 ± 0.305
3.714ProPro: 3.714 ± 0.667
2.195ProGln: 2.195 ± 0.383
3.602ProArg: 3.602 ± 0.534
3.32ProSer: 3.32 ± 0.422
3.039ProThr: 3.039 ± 0.442
4.784ProVal: 4.784 ± 0.504
0.844ProTrp: 0.844 ± 0.184
1.576ProTyr: 1.576 ± 0.279
0.0ProXaa: 0.0 ± 0.0
Gln
4.615GlnAla: 4.615 ± 0.504
0.281GlnCys: 0.281 ± 0.146
1.913GlnAsp: 1.913 ± 0.29
1.745GlnGlu: 1.745 ± 0.344
1.238GlnPhe: 1.238 ± 0.229
2.251GlnGly: 2.251 ± 0.498
0.844GlnHis: 0.844 ± 0.193
1.857GlnIle: 1.857 ± 0.342
1.238GlnLys: 1.238 ± 0.237
3.208GlnLeu: 3.208 ± 0.409
0.619GlnMet: 0.619 ± 0.176
0.844GlnAsn: 0.844 ± 0.257
2.139GlnPro: 2.139 ± 0.382
0.844GlnGln: 0.844 ± 0.226
2.307GlnArg: 2.307 ± 0.401
2.476GlnSer: 2.476 ± 0.393
1.745GlnThr: 1.745 ± 0.371
2.476GlnVal: 2.476 ± 0.38
0.619GlnTrp: 0.619 ± 0.174
0.844GlnTyr: 0.844 ± 0.272
0.0GlnXaa: 0.0 ± 0.0
Arg
6.585ArgAla: 6.585 ± 0.638
1.126ArgCys: 1.126 ± 0.298
4.446ArgAsp: 4.446 ± 0.563
4.784ArgGlu: 4.784 ± 0.605
2.364ArgPhe: 2.364 ± 0.352
4.333ArgGly: 4.333 ± 0.434
1.351ArgHis: 1.351 ± 0.34
3.996ArgIle: 3.996 ± 0.6
2.307ArgLys: 2.307 ± 0.43
4.727ArgLeu: 4.727 ± 0.664
2.251ArgMet: 2.251 ± 0.373
2.589ArgAsn: 2.589 ± 0.405
3.32ArgPro: 3.32 ± 0.461
1.688ArgGln: 1.688 ± 0.325
6.303ArgArg: 6.303 ± 1.001
3.883ArgSer: 3.883 ± 0.425
3.377ArgThr: 3.377 ± 0.472
5.459ArgVal: 5.459 ± 0.681
1.97ArgTrp: 1.97 ± 0.319
1.745ArgTyr: 1.745 ± 0.32
0.0ArgXaa: 0.0 ± 0.0
Ser
6.022SerAla: 6.022 ± 0.965
0.507SerCys: 0.507 ± 0.183
4.108SerAsp: 4.108 ± 0.524
3.433SerGlu: 3.433 ± 0.363
2.026SerPhe: 2.026 ± 0.446
6.247SerGly: 6.247 ± 0.805
1.069SerHis: 1.069 ± 0.189
2.701SerIle: 2.701 ± 0.379
2.251SerLys: 2.251 ± 0.376
3.939SerLeu: 3.939 ± 0.523
1.351SerMet: 1.351 ± 0.274
2.082SerAsn: 2.082 ± 0.351
3.714SerPro: 3.714 ± 0.399
2.026SerGln: 2.026 ± 0.303
3.602SerArg: 3.602 ± 0.45
3.546SerSer: 3.546 ± 0.656
3.602SerThr: 3.602 ± 0.427
4.615SerVal: 4.615 ± 0.441
1.351SerTrp: 1.351 ± 0.249
1.351SerTyr: 1.351 ± 0.232
0.0SerXaa: 0.0 ± 0.0
Thr
6.585ThrAla: 6.585 ± 0.562
0.788ThrCys: 0.788 ± 0.252
4.108ThrAsp: 4.108 ± 0.64
3.883ThrGlu: 3.883 ± 0.385
1.857ThrPhe: 1.857 ± 0.313
6.416ThrGly: 6.416 ± 0.784
1.913ThrHis: 1.913 ± 0.364
3.489ThrIle: 3.489 ± 0.471
1.913ThrLys: 1.913 ± 0.299
3.883ThrLeu: 3.883 ± 0.426
1.351ThrMet: 1.351 ± 0.253
2.082ThrAsn: 2.082 ± 0.313
4.221ThrPro: 4.221 ± 0.472
1.801ThrGln: 1.801 ± 0.274
4.277ThrArg: 4.277 ± 0.455
3.939ThrSer: 3.939 ± 0.516
4.615ThrThr: 4.615 ± 0.527
6.191ThrVal: 6.191 ± 0.656
1.238ThrTrp: 1.238 ± 0.234
1.913ThrTyr: 1.913 ± 0.35
0.0ThrXaa: 0.0 ± 0.0
Val
7.26ValAla: 7.26 ± 0.62
1.351ValCys: 1.351 ± 0.294
5.74ValAsp: 5.74 ± 0.59
4.502ValGlu: 4.502 ± 0.53
2.139ValPhe: 2.139 ± 0.342
5.515ValGly: 5.515 ± 0.604
1.463ValHis: 1.463 ± 0.274
2.589ValIle: 2.589 ± 0.402
2.195ValLys: 2.195 ± 0.362
5.346ValLeu: 5.346 ± 0.589
1.52ValMet: 1.52 ± 0.248
2.195ValAsn: 2.195 ± 0.357
4.559ValPro: 4.559 ± 0.37
2.701ValGln: 2.701 ± 0.347
5.009ValArg: 5.009 ± 0.607
4.446ValSer: 4.446 ± 0.477
5.459ValThr: 5.459 ± 0.594
6.134ValVal: 6.134 ± 0.673
1.576ValTrp: 1.576 ± 0.329
1.238ValTyr: 1.238 ± 0.235
0.0ValXaa: 0.0 ± 0.0
Trp
2.082TrpAla: 2.082 ± 0.311
0.394TrpCys: 0.394 ± 0.142
1.351TrpAsp: 1.351 ± 0.307
1.013TrpGlu: 1.013 ± 0.349
0.788TrpPhe: 0.788 ± 0.173
0.844TrpGly: 0.844 ± 0.202
0.619TrpHis: 0.619 ± 0.182
1.069TrpIle: 1.069 ± 0.232
0.957TrpLys: 0.957 ± 0.185
1.857TrpLeu: 1.857 ± 0.305
0.675TrpMet: 0.675 ± 0.225
0.619TrpAsn: 0.619 ± 0.231
0.957TrpPro: 0.957 ± 0.236
1.069TrpGln: 1.069 ± 0.276
2.364TrpArg: 2.364 ± 0.46
1.407TrpSer: 1.407 ± 0.314
1.576TrpThr: 1.576 ± 0.296
1.688TrpVal: 1.688 ± 0.348
0.788TrpTrp: 0.788 ± 0.184
0.507TrpTyr: 0.507 ± 0.164
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.307TyrAla: 2.307 ± 0.336
0.281TyrCys: 0.281 ± 0.128
1.688TyrAsp: 1.688 ± 0.457
2.139TyrGlu: 2.139 ± 0.346
0.788TyrPhe: 0.788 ± 0.215
2.251TyrGly: 2.251 ± 0.381
0.394TyrHis: 0.394 ± 0.138
1.013TyrIle: 1.013 ± 0.199
0.675TyrLys: 0.675 ± 0.197
2.251TyrLeu: 2.251 ± 0.379
0.225TyrMet: 0.225 ± 0.111
0.844TyrAsn: 0.844 ± 0.19
1.294TyrPro: 1.294 ± 0.202
0.788TyrGln: 0.788 ± 0.215
1.801TyrArg: 1.801 ± 0.391
0.957TyrSer: 0.957 ± 0.252
1.745TyrThr: 1.745 ± 0.299
2.251TyrVal: 2.251 ± 0.344
0.732TyrTrp: 0.732 ± 0.197
0.394TyrTyr: 0.394 ± 0.144
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 101 proteins (17770 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski