Aurantimonas aggregata
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4495 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6L9MF89|A0A6L9MF89_9RHIZ Small ribosomal subunit biogenesis GTPase RsgA OS=Aurantimonas aggregata OX=2047720 GN=rsgA PE=3 SV=1
MM1 pKa = 8.04 RR2 pKa = 11.84 ILTAAAGLALLLGTSLGAAAADD24 pKa = 3.97 VYY26 pKa = 11.4 APYY29 pKa = 10.31 DD30 pKa = 3.95 PNPAPPAPAAFDD42 pKa = 3.88 YY43 pKa = 11.4 GDD45 pKa = 3.47 TDD47 pKa = 3.9 LVIFLGIGGTVTPQYY62 pKa = 11.16 DD63 pKa = 3.46 GADD66 pKa = 3.86 DD67 pKa = 4.47 YY68 pKa = 11.41 EE69 pKa = 4.59 LSPRR73 pKa = 11.84 PIINVEE79 pKa = 3.96 YY80 pKa = 10.72 LSIPGIGAFGGKK92 pKa = 9.81 DD93 pKa = 3.19 GLGFSIGPSFGYY105 pKa = 10.24 VGEE108 pKa = 4.59 RR109 pKa = 11.84 DD110 pKa = 3.58 ASDD113 pKa = 3.86 FDD115 pKa = 4.52 GLDD118 pKa = 3.56 GLDD121 pKa = 4.89 DD122 pKa = 4.06 VDD124 pKa = 4.18 DD125 pKa = 4.33 TYY127 pKa = 11.93 EE128 pKa = 4.22 LGVRR132 pKa = 11.84 AGYY135 pKa = 9.75 EE136 pKa = 3.58 WDD138 pKa = 3.23 HH139 pKa = 8.19 AEE141 pKa = 3.52 IYY143 pKa = 10.57 GAVRR147 pKa = 11.84 YY148 pKa = 9.82 AFGGADD154 pKa = 3.41 GVVGDD159 pKa = 4.77 IGANLISRR167 pKa = 11.84 PTEE170 pKa = 3.77 RR171 pKa = 11.84 LVLKK175 pKa = 10.53 AGPTASFASSDD186 pKa = 3.6 YY187 pKa = 8.55 MDD189 pKa = 4.99 AYY191 pKa = 10.95 FGVTTSEE198 pKa = 4.2 SFNTGGRR205 pKa = 11.84 LEE207 pKa = 5.02 PYY209 pKa = 10.56 DD210 pKa = 4.05 PDD212 pKa = 4.65 GGFKK216 pKa = 10.66 SVGVAASARR225 pKa = 11.84 YY226 pKa = 8.42 EE227 pKa = 4.22 VYY229 pKa = 10.42 TDD231 pKa = 3.07 WFVDD235 pKa = 3.11 ADD237 pKa = 3.37 ASYY240 pKa = 11.75 ARR242 pKa = 11.84 LIGDD246 pKa = 4.28 AKK248 pKa = 10.77 DD249 pKa = 3.69 SPIVEE254 pKa = 4.71 AGDD257 pKa = 3.81 ANQFTFGLGVSKK269 pKa = 10.74 RR270 pKa = 11.84 FSLDD274 pKa = 2.6 LWW276 pKa = 4.14
Molecular weight: 28.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.795
IPC_protein 3.834
Toseland 3.592
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.795
Rodwell 3.656
Grimsley 3.503
Solomon 3.834
Lehninger 3.783
Nozaki 3.948
DTASelect 4.24
Thurlkill 3.656
EMBOSS 3.795
Sillero 3.961
Patrickios 1.914
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.854
Protein with the highest isoelectric point:
>tr|A0A6L9MF82|A0A6L9MF82_9RHIZ Uracil phosphoribosyltransferase OS=Aurantimonas aggregata OX=2047720 GN=upp PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 8.99 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.7 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATTGGRR28 pKa = 11.84 KK29 pKa = 9.29 VIAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.384
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4495
0
4495
1392481
34
6931
309.8
33.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.207 ± 0.055
0.779 ± 0.011
5.92 ± 0.038
5.945 ± 0.035
3.763 ± 0.024
8.844 ± 0.039
1.892 ± 0.018
5.146 ± 0.029
2.603 ± 0.025
10.064 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.45 ± 0.019
2.279 ± 0.022
5.286 ± 0.028
2.843 ± 0.019
7.432 ± 0.045
5.286 ± 0.023
5.323 ± 0.021
7.585 ± 0.031
1.252 ± 0.017
2.1 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here