Plasmodium vivax (strain Salvador I)
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5389 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A5KBW1|A5KBW1_PLAVS Uncharacterized protein OS=Plasmodium vivax (strain Salvador I) OX=126793 GN=PVX_002725 PE=4 SV=1
MM1 pKa = 7.17 KK2 pKa = 10.15 VAYY5 pKa = 9.21 FLSVLDD11 pKa = 3.94 LLIIFSLYY19 pKa = 9.97 FDD21 pKa = 3.85 GRR23 pKa = 11.84 RR24 pKa = 11.84 SAFAGIAACIRR35 pKa = 11.84 HH36 pKa = 5.14 GRR38 pKa = 11.84 ILGEE42 pKa = 4.35 GGEE45 pKa = 4.3 QSGGASGGSSGGSSGDD61 pKa = 3.33 SSGGLSGGSSGGPSPPAGSSGSGGSDD87 pKa = 3.17 PANSATGPQNSTPGSGGQTGDD108 pKa = 3.42 HH109 pKa = 6.15 SAEE112 pKa = 4.36 AEE114 pKa = 3.86 NGDD117 pKa = 4.12 YY118 pKa = 11.22 NEE120 pKa = 4.69 QGDD123 pKa = 4.6 DD124 pKa = 3.69 HH125 pKa = 8.55 GDD127 pKa = 3.53 DD128 pKa = 4.42 HH129 pKa = 8.99 GDD131 pKa = 3.66 DD132 pKa = 4.44 HH133 pKa = 8.99 GDD135 pKa = 3.66 DD136 pKa = 4.44 HH137 pKa = 8.99 GDD139 pKa = 3.66 DD140 pKa = 4.44 HH141 pKa = 8.99 GDD143 pKa = 3.66 DD144 pKa = 4.44 HH145 pKa = 8.99 GDD147 pKa = 3.66 DD148 pKa = 4.44 HH149 pKa = 8.99 GDD151 pKa = 3.66 DD152 pKa = 4.44 HH153 pKa = 8.95 GDD155 pKa = 3.55 DD156 pKa = 4.7 HH157 pKa = 9.44 GDD159 pKa = 3.53 EE160 pKa = 4.47 QDD162 pKa = 4.19 GEE164 pKa = 4.63 DD165 pKa = 4.14 YY166 pKa = 11.46 DD167 pKa = 5.29 DD168 pKa = 6.22 AEE170 pKa = 6.43 DD171 pKa = 4.75 DD172 pKa = 4.33 DD173 pKa = 6.06 LYY175 pKa = 11.42 EE176 pKa = 4.64 LSEE179 pKa = 4.04 VDD181 pKa = 4.52 EE182 pKa = 4.48 NANLCLDD189 pKa = 3.89 NNGGCGDD196 pKa = 4.83 DD197 pKa = 5.18 KK198 pKa = 11.16 ICEE201 pKa = 4.06 NLGKK205 pKa = 10.83 GIVKK209 pKa = 8.79 CLCKK213 pKa = 10.07 PGYY216 pKa = 9.93 KK217 pKa = 10.11 LVGTEE222 pKa = 4.31 CVEE225 pKa = 4.27 SSKK228 pKa = 11.15 SSSLNSFFCWFLLVIIVLASINN250 pKa = 3.49
Molecular weight: 25.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.872
IPC_protein 3.897
Toseland 3.668
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.859
Rodwell 3.719
Grimsley 3.579
Solomon 3.897
Lehninger 3.859
Nozaki 4.012
DTASelect 4.291
Thurlkill 3.732
EMBOSS 3.859
Sillero 4.024
Patrickios 1.049
IPC_peptide 3.897
IPC2_peptide 3.999
IPC2.peptide.svr19 3.9
Protein with the highest isoelectric point:
>tr|A5K5U9|A5K5U9_PLAVS WD domain G-beta repeat domain containing protein OS=Plasmodium vivax (strain Salvador I) OX=126793 GN=PVX_089605 PE=4 SV=1
MM1 pKa = 7.33 VLLKK5 pKa = 10.69 KK6 pKa = 10.46 KK7 pKa = 10.52 LLLRR11 pKa = 11.84 KK12 pKa = 9.14 PLLKK16 pKa = 10.45 RR17 pKa = 11.84 LLRR20 pKa = 11.84 KK21 pKa = 9.29 PLLKK25 pKa = 10.45 RR26 pKa = 11.84 LLRR29 pKa = 11.84 KK30 pKa = 9.29 PLLKK34 pKa = 10.5 RR35 pKa = 11.84 LLKK38 pKa = 10.61 KK39 pKa = 10.4 LLKK42 pKa = 10.17 NVPKK46 pKa = 10.45 KK47 pKa = 10.56 RR48 pKa = 11.84 SLL50 pKa = 3.41
Molecular weight: 6.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.471
IPC2_protein 11.111
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.808
Grimsley 12.852
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.305
Sillero 12.808
Patrickios 12.515
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.015
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5389
0
5389
3748164
39
11429
695.5
78.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.261 ± 0.031
1.84 ± 0.014
5.48 ± 0.026
7.71 ± 0.048
4.267 ± 0.029
6.594 ± 0.046
2.716 ± 0.016
5.595 ± 0.035
9.015 ± 0.038
8.214 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.017 ± 0.013
7.244 ± 0.039
3.608 ± 0.025
3.277 ± 0.018
4.901 ± 0.032
8.076 ± 0.032
4.3 ± 0.018
5.244 ± 0.022
0.619 ± 0.007
4.018 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here