Tetrabaena socialis
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14121 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2J8A2H0|A0A2J8A2H0_9CHLO Zinc-metallopeptidase peroxisomal (Fragment) OS=Tetrabaena socialis OX=47790 GN=TSOC_006881 PE=4 SV=1
MM1 pKa = 7.52 PTPFDD6 pKa = 3.74 APAPKK11 pKa = 9.73 PATDD15 pKa = 4.03 TSCIAAAEE23 pKa = 4.18 EE24 pKa = 4.25 CGAAEE29 pKa = 4.04 TDD31 pKa = 3.54 PVVAHH36 pKa = 5.47 VPVWASEE43 pKa = 4.02 DD44 pKa = 3.72 GTVVFADD51 pKa = 3.49 AQGNEE56 pKa = 3.86 VRR58 pKa = 11.84 VGDD61 pKa = 3.9 VFGAASEE68 pKa = 4.29 LTGFMDD74 pKa = 2.98 EE75 pKa = 4.73 HH76 pKa = 6.64 GAVAYY81 pKa = 9.15 YY82 pKa = 10.28 VVIRR86 pKa = 11.84 QSRR89 pKa = 11.84 MVAHH93 pKa = 7.18 AAHH96 pKa = 7.12 LEE98 pKa = 3.94 QLEE101 pKa = 4.38 AEE103 pKa = 4.31 QHH105 pKa = 5.06 QLLLGTSAGVDD116 pKa = 3.08 ADD118 pKa = 3.63 ADD120 pKa = 3.86 AGHH123 pKa = 6.23 VVASGGGAEE132 pKa = 6.08 DD133 pKa = 4.48 EE134 pKa = 5.33 DD135 pKa = 6.25 LDD137 pKa = 5.54 DD138 pKa = 6.53 LLALCMGGGGAIPSVVDD155 pKa = 3.18 YY156 pKa = 10.94 GAATMQYY163 pKa = 9.29 TDD165 pKa = 4.4 LSAAAILGFEE175 pKa = 5.57 DD176 pKa = 4.86 MMQLLCVV183 pKa = 3.69
Molecular weight: 18.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.567
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.101
Thurlkill 3.617
EMBOSS 3.706
Sillero 3.897
Patrickios 0.566
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.809
Protein with the highest isoelectric point:
>tr|A0A2J7ZXE9|A0A2J7ZXE9_9CHLO Uncharacterized protein (Fragment) OS=Tetrabaena socialis OX=47790 GN=TSOC_008879 PE=4 SV=1
MM1 pKa = 7.72 APRR4 pKa = 11.84 RR5 pKa = 11.84 LAASRR10 pKa = 11.84 RR11 pKa = 11.84 SSRR14 pKa = 11.84 SSGNSGSSLLLGPRR28 pKa = 11.84 SSRR31 pKa = 11.84 SRR33 pKa = 11.84 SRR35 pKa = 11.84 VMRR38 pKa = 11.84 RR39 pKa = 11.84 QARR42 pKa = 11.84 SRR44 pKa = 11.84 SSARR48 pKa = 11.84 SKK50 pKa = 9.66 RR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 RR55 pKa = 11.84 RR56 pKa = 11.84 RR57 pKa = 11.84 PQSWPGQHH65 pKa = 6.41 PTTSTLAPRR74 pKa = 11.84 LTAAQQWRR82 pKa = 11.84 TWAWRR87 pKa = 11.84 MRR89 pKa = 11.84 TQASPRR95 pKa = 11.84 QPRR98 pKa = 11.84 RR99 pKa = 11.84 RR100 pKa = 11.84 CPSSKK105 pKa = 9.09 IRR107 pKa = 11.84 RR108 pKa = 11.84 RR109 pKa = 11.84 SRR111 pKa = 11.84 RR112 pKa = 11.84 RR113 pKa = 11.84 SS114 pKa = 3.18
Molecular weight: 13.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.513
IPC2_protein 11.447
IPC_protein 13.056
Toseland 13.217
ProMoST 13.715
Dawson 13.217
Bjellqvist 13.217
Wikipedia 13.7
Rodwell 12.749
Grimsley 13.261
Solomon 13.715
Lehninger 13.612
Nozaki 13.217
DTASelect 13.217
Thurlkill 13.217
EMBOSS 13.715
Sillero 13.217
Patrickios 12.457
IPC_peptide 13.715
IPC2_peptide 12.705
IPC2.peptide.svr19 9.346
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14121
0
14121
5766222
11
10535
408.3
42.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
15.192 ± 0.053
1.738 ± 0.012
4.477 ± 0.017
5.176 ± 0.025
2.396 ± 0.015
10.187 ± 0.038
2.221 ± 0.012
2.378 ± 0.017
2.753 ± 0.022
9.677 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.979 ± 0.01
2.071 ± 0.015
7.045 ± 0.032
4.399 ± 0.027
6.853 ± 0.024
6.998 ± 0.024
4.611 ± 0.017
6.527 ± 0.019
1.367 ± 0.007
1.951 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here