Terrimonas sp. NS-102
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5019 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T7Q2E9|A0A2T7Q2E9_9BACT IS66 family transposase OS=Terrimonas sp. NS-102 OX=2171976 GN=DC498_24570 PE=4 SV=1
MM1 pKa = 7.96 RR2 pKa = 11.84 YY3 pKa = 9.61 IIFTILLFVQLGVYY17 pKa = 9.17 AQSTGDD23 pKa = 3.63 YY24 pKa = 10.08 RR25 pKa = 11.84 SKK27 pKa = 11.03 QSGNWEE33 pKa = 4.06 DD34 pKa = 3.29 AGSWEE39 pKa = 4.63 TYY41 pKa = 10.85 NGTSWVAATNYY52 pKa = 6.7 PTPSDD57 pKa = 3.39 GTITIRR63 pKa = 11.84 SPHH66 pKa = 7.0 ILTQGSSFTINDD78 pKa = 3.06 VTVEE82 pKa = 4.04 VGATLNLNDD91 pKa = 4.25 GNVNSSGSSTADD103 pKa = 2.98 LRR105 pKa = 11.84 VYY107 pKa = 9.73 GTVNHH112 pKa = 6.51 NANSQGGCPIFEE124 pKa = 4.43 IYY126 pKa = 10.56 NGGVYY131 pKa = 9.86 NWNGGNYY138 pKa = 9.88 ACNTIKK144 pKa = 10.42 ILSGGTMNFNVSGNPYY160 pKa = 10.46 LNEE163 pKa = 4.01 TNITNDD169 pKa = 2.85 GVINFNSGGFYY180 pKa = 10.58 AAINTVWGNLVNNAGGVINKK200 pKa = 9.61 NNDD203 pKa = 3.37 NIFFASGSPFNFIQNGSLNINAGRR227 pKa = 11.84 LHH229 pKa = 7.04 IDD231 pKa = 3.13 YY232 pKa = 10.96 LNFSNTGQLSIANNAEE248 pKa = 4.5 LVCSGTPLMLSGTLNVIEE266 pKa = 5.0 KK267 pKa = 10.04 VSPSNGSNVIISGNFSGNFSTVNLPIGYY295 pKa = 9.34 SITVNPSDD303 pKa = 5.13 VILNYY308 pKa = 10.39 NDD310 pKa = 5.48 DD311 pKa = 3.79 MDD313 pKa = 6.87 DD314 pKa = 4.84 DD315 pKa = 4.52 GVKK318 pKa = 10.76 NKK320 pKa = 10.21 DD321 pKa = 3.06 DD322 pKa = 4.63 CAPKK326 pKa = 10.61 DD327 pKa = 3.64 PNKK330 pKa = 9.35 WRR332 pKa = 11.84 SAEE335 pKa = 4.02 FYY337 pKa = 10.18 IDD339 pKa = 4.17 KK340 pKa = 11.01 DD341 pKa = 3.26 SDD343 pKa = 3.91 GYY345 pKa = 11.37 DD346 pKa = 3.44 GGKK349 pKa = 8.24 HH350 pKa = 4.2 TVCYY354 pKa = 9.37 GQNIPSGYY362 pKa = 9.46 IQTTKK367 pKa = 10.87 GSDD370 pKa = 3.87 CNDD373 pKa = 2.85 NDD375 pKa = 4.55 ANINPTTVWYY385 pKa = 10.62 KK386 pKa = 10.87 DD387 pKa = 3.35 ADD389 pKa = 3.62 NDD391 pKa = 4.49 GYY393 pKa = 11.71 SDD395 pKa = 3.8 GTTKK399 pKa = 8.92 TQCDD403 pKa = 3.31 QPAGYY408 pKa = 9.59 KK409 pKa = 10.36 LKK411 pKa = 10.9 AQLTATNGDD420 pKa = 4.3 CKK422 pKa = 11.18 DD423 pKa = 4.15 DD424 pKa = 4.65 DD425 pKa = 4.08 ATIHH429 pKa = 6.62 PGAPEE434 pKa = 3.61 ICGNGIDD441 pKa = 4.59 EE442 pKa = 5.21 DD443 pKa = 4.77 CDD445 pKa = 4.1 SKK447 pKa = 11.74 DD448 pKa = 3.62 AVCVPTDD455 pKa = 3.29 SDD457 pKa = 4.03 GDD459 pKa = 4.09 GVSDD463 pKa = 6.01 NEE465 pKa = 4.44 DD466 pKa = 3.67 CSPNDD471 pKa = 3.21 NKK473 pKa = 10.15 VWRR476 pKa = 11.84 TVTLYY481 pKa = 11.25 ADD483 pKa = 4.38 FDD485 pKa = 5.08 SDD487 pKa = 3.78 GKK489 pKa = 9.87 PVAFGSEE496 pKa = 4.09 VCIGADD502 pKa = 2.94 IPQGYY507 pKa = 9.11 SEE509 pKa = 5.4 SPGSDD514 pKa = 3.51 CDD516 pKa = 5.54 DD517 pKa = 3.82 NDD519 pKa = 3.41 NTVWRR524 pKa = 11.84 TAILYY529 pKa = 9.88 IDD531 pKa = 3.97 SDD533 pKa = 4.05 RR534 pKa = 11.84 DD535 pKa = 3.85 GEE537 pKa = 4.68 SVGAGIEE544 pKa = 4.0 KK545 pKa = 10.57 CIGNDD550 pKa = 2.72 IPFGYY555 pKa = 8.87 TEE557 pKa = 5.11 SPGSDD562 pKa = 3.48 CNDD565 pKa = 3.49 NNPDD569 pKa = 3.4 IYY571 pKa = 10.92 HH572 pKa = 7.0 GATEE576 pKa = 4.12 ICDD579 pKa = 3.84 GVDD582 pKa = 3.45 NNCDD586 pKa = 3.4 GQIDD590 pKa = 4.0 EE591 pKa = 5.99 GLLFWIYY598 pKa = 10.46 PDD600 pKa = 4.36 GDD602 pKa = 3.45 GDD604 pKa = 4.53 GYY606 pKa = 10.2 GTEE609 pKa = 3.84 EE610 pKa = 4.24 GKK612 pKa = 10.29 IYY614 pKa = 10.48 SCNAPYY620 pKa = 10.55 GYY622 pKa = 10.84 ADD624 pKa = 4.5 RR625 pKa = 11.84 NGDD628 pKa = 4.75 CKK630 pKa = 11.31 DD631 pKa = 4.04 DD632 pKa = 5.09 DD633 pKa = 3.84 NTINPGVEE641 pKa = 4.44 EE642 pKa = 4.3 ICDD645 pKa = 3.73 DD646 pKa = 5.88 GIDD649 pKa = 3.79 NDD651 pKa = 4.45 CDD653 pKa = 4.12 GEE655 pKa = 4.05 IDD657 pKa = 3.74 EE658 pKa = 5.22 GCSVSEE664 pKa = 3.8 PTEE667 pKa = 4.71 FYY669 pKa = 11.1 SKK671 pKa = 9.55 PTGDD675 pKa = 3.76 LHH677 pKa = 7.35 NVATWGVNPDD687 pKa = 3.35 GSGTQPADD695 pKa = 3.86 FGAGKK700 pKa = 8.29 TFNLANRR707 pKa = 11.84 AGNYY711 pKa = 8.47 TMTGNWTVLGTLVNSSGSQLKK732 pKa = 10.43 INGYY736 pKa = 6.21 TLSLTTLTGAGTLTGSTTSSLIITGTGGGNFGNINFTSGGGMLKK780 pKa = 10.6 AFTLNRR786 pKa = 11.84 SGTGAAATIGTALAVYY802 pKa = 9.99 DD803 pKa = 4.18 VLTITSGALTTGGKK817 pKa = 8.08 LTLKK821 pKa = 9.52 STATNTARR829 pKa = 11.84 VAPVTGTISGNVTVEE844 pKa = 3.79 RR845 pKa = 11.84 YY846 pKa = 9.59 IPARR850 pKa = 11.84 RR851 pKa = 11.84 AWRR854 pKa = 11.84 LMNAPVGGTQTINQAWQEE872 pKa = 4.27 GVTTASPNPNPAPGYY887 pKa = 7.66 GTYY890 pKa = 8.98 VTVGSVANGFDD901 pKa = 4.01 QNILGQSTSSLKK913 pKa = 10.96 SFF915 pKa = 4.16
Molecular weight: 96.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.81
IPC2_protein 3.91
IPC_protein 3.973
Toseland 3.732
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.113
Wikipedia 3.91
Rodwell 3.783
Grimsley 3.63
Solomon 3.961
Lehninger 3.923
Nozaki 4.075
DTASelect 4.368
Thurlkill 3.783
EMBOSS 3.923
Sillero 4.088
Patrickios 0.591
IPC_peptide 3.961
IPC2_peptide 4.062
IPC2.peptide.svr19 3.956
Protein with the highest isoelectric point:
>tr|A0A2T7QCI3|A0A2T7QCI3_9BACT Peptidase_M56 domain-containing protein OS=Terrimonas sp. NS-102 OX=2171976 GN=DC498_06575 PE=4 SV=1
MM1 pKa = 7.49 FLFLIDD7 pKa = 4.05 CNKK10 pKa = 9.9 GHH12 pKa = 7.45 ACRR15 pKa = 11.84 SFRR18 pKa = 11.84 QPAIFLYY25 pKa = 10.34 RR26 pKa = 11.84 YY27 pKa = 7.86 LQKK30 pKa = 10.33 PARR33 pKa = 11.84 ARR35 pKa = 11.84 DD36 pKa = 3.45 LQYY39 pKa = 10.85 IQLKK43 pKa = 10.22 EE44 pKa = 3.88 MLNNGLLSLVFSKK57 pKa = 10.83 YY58 pKa = 10.34 KK59 pKa = 10.13 QVALQKK65 pKa = 10.04 RR66 pKa = 11.84 GYY68 pKa = 8.03 TRR70 pKa = 11.84 GVYY73 pKa = 10.47 LIISNITFRR82 pKa = 11.84 LLHH85 pKa = 5.5 NHH87 pKa = 5.4 SCRR90 pKa = 11.84 SFKK93 pKa = 10.97
Molecular weight: 11.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.94
IPC_protein 10.511
Toseland 10.452
ProMoST 10.233
Dawson 10.628
Bjellqvist 10.335
Wikipedia 10.833
Rodwell 10.979
Grimsley 10.701
Solomon 10.672
Lehninger 10.643
Nozaki 10.467
DTASelect 10.335
Thurlkill 10.496
EMBOSS 10.847
Sillero 10.555
Patrickios 10.687
IPC_peptide 10.672
IPC2_peptide 9.414
IPC2.peptide.svr19 8.385
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5019
0
5019
1778361
16
2923
354.3
39.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.511 ± 0.035
0.861 ± 0.011
5.302 ± 0.024
5.592 ± 0.031
4.885 ± 0.023
6.835 ± 0.033
1.855 ± 0.019
7.55 ± 0.031
7.222 ± 0.03
9.018 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.306 ± 0.017
5.715 ± 0.03
3.871 ± 0.022
3.678 ± 0.019
3.93 ± 0.021
6.304 ± 0.026
5.768 ± 0.033
6.195 ± 0.024
1.339 ± 0.013
4.262 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here