Bark beetle-associated genomovirus 4

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus denbre4

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A344A3Q3|A0A344A3Q3_9VIRU Replication-associated protein OS=Bark beetle-associated genomovirus 4 OX=2230899 PE=3 SV=1
MM1 pKa = 7.94PFHH4 pKa = 7.01CNARR8 pKa = 11.84YY9 pKa = 8.94FLVTYY14 pKa = 9.34SHH16 pKa = 6.96VEE18 pKa = 3.79PLDD21 pKa = 3.65PFALVEE27 pKa = 4.35HH28 pKa = 6.73FGSLGAEE35 pKa = 3.87IIVSLEE41 pKa = 3.82QYY43 pKa = 10.33NATLGVHH50 pKa = 5.02YY51 pKa = 10.39HH52 pKa = 5.64VFADD56 pKa = 4.64FGRR59 pKa = 11.84KK60 pKa = 8.16FRR62 pKa = 11.84SRR64 pKa = 11.84RR65 pKa = 11.84TDD67 pKa = 2.68IFDD70 pKa = 3.27VDD72 pKa = 3.91GFHH75 pKa = 7.48PNISPSRR82 pKa = 11.84GTPEE86 pKa = 3.98AGYY89 pKa = 10.62DD90 pKa = 3.63YY91 pKa = 10.68AIKK94 pKa = 10.92DD95 pKa = 3.59GDD97 pKa = 4.02VVAGGLARR105 pKa = 11.84PSGVGATGRR114 pKa = 11.84AAKK117 pKa = 8.68WHH119 pKa = 6.74SILDD123 pKa = 3.54AEE125 pKa = 4.54TRR127 pKa = 11.84DD128 pKa = 3.66EE129 pKa = 5.25FFALCEE135 pKa = 3.97EE136 pKa = 5.06LDD138 pKa = 3.88PEE140 pKa = 4.71RR141 pKa = 11.84LVCSFGQIQKK151 pKa = 9.89FADD154 pKa = 2.98WRR156 pKa = 11.84FAPVAQPYY164 pKa = 9.99ASPDD168 pKa = 3.43GVFDD172 pKa = 4.25LANYY176 pKa = 9.89GDD178 pKa = 3.96LADD181 pKa = 3.68WRR183 pKa = 11.84DD184 pKa = 3.53NFLVADD190 pKa = 3.67TAGRR194 pKa = 11.84SKK196 pKa = 11.1SLILWGPSRR205 pKa = 11.84MGKK208 pKa = 6.7TVWGRR213 pKa = 11.84SLGNHH218 pKa = 7.09LYY220 pKa = 10.71FGGIFSARR228 pKa = 11.84DD229 pKa = 3.28INRR232 pKa = 11.84DD233 pKa = 3.09GVEE236 pKa = 3.89YY237 pKa = 10.85AVFDD241 pKa = 5.39DD242 pKa = 3.46IAGGIKK248 pKa = 10.03FFPRR252 pKa = 11.84FKK254 pKa = 10.79DD255 pKa = 3.12WLGCQMEE262 pKa = 4.36FMVKK266 pKa = 9.32EE267 pKa = 4.5MYY269 pKa = 10.19RR270 pKa = 11.84DD271 pKa = 3.27PHH273 pKa = 6.35LFKK276 pKa = 10.36WGRR279 pKa = 11.84PAIWIANSDD288 pKa = 3.56PRR290 pKa = 11.84HH291 pKa = 6.58DD292 pKa = 3.96MTHH295 pKa = 7.62DD296 pKa = 5.0DD297 pKa = 4.02ITWLEE302 pKa = 4.01ANCIFVEE309 pKa = 4.21ISSPIFHH316 pKa = 7.4ANTEE320 pKa = 4.14

Molecular weight: 36.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A344A3Q3|A0A344A3Q3_9VIRU Replication-associated protein OS=Bark beetle-associated genomovirus 4 OX=2230899 PE=3 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84SRR5 pKa = 11.84YY6 pKa = 9.44YY7 pKa = 10.68GGKK10 pKa = 8.05KK11 pKa = 4.93TTGWANSRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84KK22 pKa = 9.79SRR24 pKa = 11.84SKK26 pKa = 10.32PRR28 pKa = 11.84RR29 pKa = 11.84SYY31 pKa = 10.73AKK33 pKa = 9.56KK34 pKa = 9.89RR35 pKa = 11.84STFRR39 pKa = 11.84PRR41 pKa = 11.84KK42 pKa = 7.11MSRR45 pKa = 11.84RR46 pKa = 11.84SILNVTSRR54 pKa = 11.84KK55 pKa = 9.78KK56 pKa = 9.85QDD58 pKa = 3.86NMLSWSNKK66 pKa = 8.54NNAGNNQAVATGGLYY81 pKa = 10.82VNGLAGYY88 pKa = 7.97SMCVFCPTARR98 pKa = 11.84SLTTASATNLLVDD111 pKa = 3.53QADD114 pKa = 4.23RR115 pKa = 11.84TSTTCFMRR123 pKa = 11.84GYY125 pKa = 10.29KK126 pKa = 10.04EE127 pKa = 3.71NLRR130 pKa = 11.84IQTSSPLPWLWRR142 pKa = 11.84RR143 pKa = 11.84IVFATKK149 pKa = 10.33GPTFYY154 pKa = 10.96VSSAADD160 pKa = 3.4TPTIKK165 pKa = 9.73FTPYY169 pKa = 10.18SDD171 pKa = 3.5TSVGMARR178 pKa = 11.84LWFDD182 pKa = 3.49LQYY185 pKa = 10.82NSSPATVKK193 pKa = 10.82AMNTIIFKK201 pKa = 10.82GNQDD205 pKa = 3.41QDD207 pKa = 3.47WNDD210 pKa = 3.32VITAKK215 pKa = 10.23VDD217 pKa = 3.47PSRR220 pKa = 11.84ITVMSDD226 pKa = 2.97RR227 pKa = 11.84TCAIKK232 pKa = 8.07TTNSNGHH239 pKa = 4.88FSEE242 pKa = 4.42RR243 pKa = 11.84KK244 pKa = 8.83LWYY247 pKa = 9.61PMNKK251 pKa = 9.49NLVYY255 pKa = 10.91DD256 pKa = 4.4DD257 pKa = 5.44DD258 pKa = 4.18EE259 pKa = 6.9AGAAVASSYY268 pKa = 11.32YY269 pKa = 9.95STDD272 pKa = 3.1AKK274 pKa = 10.71PGMGDD279 pKa = 3.51VYY281 pKa = 11.2VVDD284 pKa = 3.95YY285 pKa = 9.75VVPGVGGATSDD296 pKa = 3.75IINFNATATLYY307 pKa = 9.38WHH309 pKa = 7.04EE310 pKa = 4.29KK311 pKa = 9.3

Molecular weight: 34.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

631

311

320

315.5

35.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.875 ± 0.136

1.426 ± 0.099

7.29 ± 1.058

3.17 ± 1.326

5.705 ± 1.753

7.448 ± 0.943

2.219 ± 1.109

4.596 ± 0.745

4.913 ± 1.295

5.705 ± 0.621

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.479

4.913 ± 1.295

4.754 ± 0.404

1.902 ± 0.246

7.132 ± 0.412

7.765 ± 1.55

6.498 ± 2.217

6.181 ± 0.176

2.694 ± 0.086

4.279 ± 0.609
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski