Cellulomonas sp. PhB143

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Cellulomonadaceae; Cellulomonas; unclassified Cellulomonas

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2804 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N2JIW8|A0A3N2JIW8_9CELL GAF domain-containing protein OS=Cellulomonas sp. PhB143 OX=2485186 GN=EDF32_2585 PE=4 SV=1
MM1 pKa = 7.7PSDD4 pKa = 4.56AFPEE8 pKa = 4.06NLRR11 pKa = 11.84YY12 pKa = 7.74TAEE15 pKa = 4.44HH16 pKa = 6.27EE17 pKa = 4.64WIDD20 pKa = 3.89DD21 pKa = 3.81ASPATVGITANAAEE35 pKa = 4.24ALGDD39 pKa = 3.64IVYY42 pKa = 10.88LEE44 pKa = 4.31LPEE47 pKa = 4.35VGAALTAGEE56 pKa = 4.31VFGEE60 pKa = 4.17IEE62 pKa = 4.18STKK65 pKa = 10.64SVSEE69 pKa = 4.28LYY71 pKa = 10.86APVTGTVVEE80 pKa = 4.52INQEE84 pKa = 3.94AVDD87 pKa = 3.78APEE90 pKa = 4.37TVGSDD95 pKa = 3.34PYY97 pKa = 10.78GAGWLVKK104 pKa = 10.34VDD106 pKa = 3.68VSEE109 pKa = 4.42VGAVLTAEE117 pKa = 4.73EE118 pKa = 4.19YY119 pKa = 9.84RR120 pKa = 11.84AQVGGG125 pKa = 3.59

Molecular weight: 13.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N2JUZ5|A0A3N2JUZ5_9CELL Galactokinase OS=Cellulomonas sp. PhB143 OX=2485186 GN=EDF32_0827 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2804

0

2804

979563

32

2811

349.3

36.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.523 ± 0.073

0.563 ± 0.011

6.744 ± 0.043

5.305 ± 0.051

2.537 ± 0.028

9.801 ± 0.046

2.018 ± 0.019

2.808 ± 0.039

1.57 ± 0.029

9.85 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.544 ± 0.019

1.452 ± 0.021

5.92 ± 0.036

2.529 ± 0.025

7.855 ± 0.063

5.22 ± 0.038

6.242 ± 0.052

10.19 ± 0.048

1.481 ± 0.02

1.846 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski