Amino acid dipepetide frequency for Cellulomonas sp. PhB143

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.367AlaAla: 23.367 ± 0.284
0.833AlaCys: 0.833 ± 0.03
9.288AlaAsp: 9.288 ± 0.129
8.038AlaGlu: 8.038 ± 0.11
3.446AlaPhe: 3.446 ± 0.065
14.935AlaGly: 14.935 ± 0.143
2.738AlaHis: 2.738 ± 0.065
3.714AlaIle: 3.714 ± 0.076
2.224AlaLys: 2.224 ± 0.061
14.403AlaLeu: 14.403 ± 0.153
2.505AlaMet: 2.505 ± 0.058
1.768AlaAsn: 1.768 ± 0.046
8.203AlaPro: 8.203 ± 0.125
4.187AlaGln: 4.187 ± 0.073
11.727AlaArg: 11.727 ± 0.153
7.244AlaSer: 7.244 ± 0.102
8.295AlaThr: 8.295 ± 0.116
13.792AlaVal: 13.792 ± 0.156
2.132AlaTrp: 2.132 ± 0.054
2.394AlaTyr: 2.394 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
0.784CysAla: 0.784 ± 0.031
0.071CysCys: 0.071 ± 0.009
0.368CysAsp: 0.368 ± 0.02
0.29CysGlu: 0.29 ± 0.018
0.17CysPhe: 0.17 ± 0.012
0.676CysGly: 0.676 ± 0.026
0.112CysHis: 0.112 ± 0.009
0.137CysIle: 0.137 ± 0.012
0.056CysLys: 0.056 ± 0.008
0.484CysLeu: 0.484 ± 0.022
0.07CysMet: 0.07 ± 0.008
0.07CysAsn: 0.07 ± 0.008
0.357CysPro: 0.357 ± 0.02
0.108CysGln: 0.108 ± 0.012
0.37CysArg: 0.37 ± 0.018
0.344CysSer: 0.344 ± 0.023
0.411CysThr: 0.411 ± 0.025
0.565CysVal: 0.565 ± 0.024
0.08CysTrp: 0.08 ± 0.011
0.104CysTyr: 0.104 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
10.702AspAla: 10.702 ± 0.128
0.272AspCys: 0.272 ± 0.018
5.625AspAsp: 5.625 ± 0.086
4.172AspGlu: 4.172 ± 0.072
1.326AspPhe: 1.326 ± 0.038
7.777AspGly: 7.777 ± 0.12
1.303AspHis: 1.303 ± 0.037
1.328AspIle: 1.328 ± 0.037
0.874AspLys: 0.874 ± 0.03
6.903AspLeu: 6.903 ± 0.082
0.715AspMet: 0.715 ± 0.026
0.739AspAsn: 0.739 ± 0.031
4.892AspPro: 4.892 ± 0.082
1.581AspGln: 1.581 ± 0.041
4.702AspArg: 4.702 ± 0.063
2.219AspSer: 2.219 ± 0.058
2.782AspThr: 2.782 ± 0.062
7.547AspVal: 7.547 ± 0.099
0.879AspTrp: 0.879 ± 0.029
1.1AspTyr: 1.1 ± 0.041
0.0AspXaa: 0.0 ± 0.0
Glu
7.025GluAla: 7.025 ± 0.104
0.234GluCys: 0.234 ± 0.017
2.973GluAsp: 2.973 ± 0.062
2.585GluGlu: 2.585 ± 0.063
1.14GluPhe: 1.14 ± 0.035
4.054GluGly: 4.054 ± 0.077
1.724GluHis: 1.724 ± 0.048
2.229GluIle: 2.229 ± 0.052
1.046GluLys: 1.046 ± 0.037
5.966GluLeu: 5.966 ± 0.09
0.78GluMet: 0.78 ± 0.029
0.886GluAsn: 0.886 ± 0.031
3.359GluPro: 3.359 ± 0.073
1.934GluGln: 1.934 ± 0.053
5.514GluArg: 5.514 ± 0.095
2.401GluSer: 2.401 ± 0.05
2.744GluThr: 2.744 ± 0.053
4.972GluVal: 4.972 ± 0.093
0.603GluTrp: 0.603 ± 0.024
0.88GluTyr: 0.88 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
3.618PheAla: 3.618 ± 0.073
0.189PheCys: 0.189 ± 0.014
1.987PheAsp: 1.987 ± 0.051
1.414PheGlu: 1.414 ± 0.048
0.85PhePhe: 0.85 ± 0.032
2.802PheGly: 2.802 ± 0.062
0.487PheHis: 0.487 ± 0.025
0.572PheIle: 0.572 ± 0.023
0.333PheLys: 0.333 ± 0.021
2.248PheLeu: 2.248 ± 0.053
0.357PheMet: 0.357 ± 0.021
0.441PheAsn: 0.441 ± 0.024
1.228PhePro: 1.228 ± 0.036
0.57PheGln: 0.57 ± 0.022
1.559PheArg: 1.559 ± 0.043
1.41PheSer: 1.41 ± 0.041
1.662PheThr: 1.662 ± 0.052
2.68PheVal: 2.68 ± 0.06
0.44PheTrp: 0.44 ± 0.022
0.529PheTyr: 0.529 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
13.583GlyAla: 13.583 ± 0.153
0.616GlyCys: 0.616 ± 0.029
5.809GlyAsp: 5.809 ± 0.095
4.792GlyGlu: 4.792 ± 0.065
2.803GlyPhe: 2.803 ± 0.05
9.048GlyGly: 9.048 ± 0.144
1.915GlyHis: 1.915 ± 0.049
3.338GlyIle: 3.338 ± 0.071
1.967GlyLys: 1.967 ± 0.047
9.456GlyLeu: 9.456 ± 0.099
1.838GlyMet: 1.838 ± 0.053
1.38GlyAsn: 1.38 ± 0.041
5.512GlyPro: 5.512 ± 0.092
2.43GlyGln: 2.43 ± 0.048
7.733GlyArg: 7.733 ± 0.117
5.932GlySer: 5.932 ± 0.104
7.183GlyThr: 7.183 ± 0.113
8.726GlyVal: 8.726 ± 0.1
1.785GlyTrp: 1.785 ± 0.047
2.162GlyTyr: 2.162 ± 0.056
0.0GlyXaa: 0.0 ± 0.0
His
2.714HisAla: 2.714 ± 0.068
0.112HisCys: 0.112 ± 0.011
1.479HisAsp: 1.479 ± 0.037
1.175HisGlu: 1.175 ± 0.033
0.428HisPhe: 0.428 ± 0.023
2.176HisGly: 2.176 ± 0.053
0.553HisHis: 0.553 ± 0.026
0.375HisIle: 0.375 ± 0.018
0.224HisLys: 0.224 ± 0.016
2.251HisLeu: 2.251 ± 0.054
0.234HisMet: 0.234 ± 0.017
0.301HisAsn: 0.301 ± 0.021
1.51HisPro: 1.51 ± 0.043
0.485HisGln: 0.485 ± 0.022
1.791HisArg: 1.791 ± 0.042
0.754HisSer: 0.754 ± 0.028
0.99HisThr: 0.99 ± 0.029
2.019HisVal: 2.019 ± 0.056
0.243HisTrp: 0.243 ± 0.017
0.365HisTyr: 0.365 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
3.987IleAla: 3.987 ± 0.072
0.165IleCys: 0.165 ± 0.013
2.371IleAsp: 2.371 ± 0.053
1.804IleGlu: 1.804 ± 0.055
0.761IlePhe: 0.761 ± 0.031
3.072IleGly: 3.072 ± 0.067
0.488IleHis: 0.488 ± 0.026
0.8IleIle: 0.8 ± 0.031
0.566IleLys: 0.566 ± 0.027
2.352IleLeu: 2.352 ± 0.067
0.398IleMet: 0.398 ± 0.023
0.53IleAsn: 0.53 ± 0.021
1.385IlePro: 1.385 ± 0.042
0.58IleGln: 0.58 ± 0.026
1.529IleArg: 1.529 ± 0.043
1.463IleSer: 1.463 ± 0.041
1.984IleThr: 1.984 ± 0.053
3.067IleVal: 3.067 ± 0.062
0.31IleTrp: 0.31 ± 0.017
0.464IleTyr: 0.464 ± 0.022
0.0IleXaa: 0.0 ± 0.0
Lys
2.036LysAla: 2.036 ± 0.061
0.063LysCys: 0.063 ± 0.009
1.229LysAsp: 1.229 ± 0.042
0.811LysGlu: 0.811 ± 0.033
0.335LysPhe: 0.335 ± 0.017
1.382LysGly: 1.382 ± 0.042
0.361LysHis: 0.361 ± 0.017
0.766LysIle: 0.766 ± 0.03
0.609LysLys: 0.609 ± 0.031
1.202LysLeu: 1.202 ± 0.041
0.249LysMet: 0.249 ± 0.016
0.379LysAsn: 0.379 ± 0.022
0.863LysPro: 0.863 ± 0.037
0.504LysGln: 0.504 ± 0.023
0.951LysArg: 0.951 ± 0.036
0.901LysSer: 0.901 ± 0.035
0.973LysThr: 0.973 ± 0.04
1.594LysVal: 1.594 ± 0.043
0.137LysTrp: 0.137 ± 0.011
0.359LysTyr: 0.359 ± 0.022
0.0LysXaa: 0.0 ± 0.0
Leu
15.968LeuAla: 15.968 ± 0.171
0.547LeuCys: 0.547 ± 0.023
7.162LeuAsp: 7.162 ± 0.09
5.152LeuGlu: 5.152 ± 0.095
2.235LeuPhe: 2.235 ± 0.059
9.723LeuGly: 9.723 ± 0.125
1.867LeuHis: 1.867 ± 0.05
2.102LeuIle: 2.102 ± 0.061
1.292LeuLys: 1.292 ± 0.042
9.926LeuLeu: 9.926 ± 0.146
1.308LeuMet: 1.308 ± 0.038
1.274LeuAsn: 1.274 ± 0.041
5.561LeuPro: 5.561 ± 0.086
2.015LeuGln: 2.015 ± 0.043
7.736LeuArg: 7.736 ± 0.105
4.686LeuSer: 4.686 ± 0.078
5.894LeuThr: 5.894 ± 0.072
11.329LeuVal: 11.329 ± 0.143
1.211LeuTrp: 1.211 ± 0.036
1.514LeuTyr: 1.514 ± 0.041
0.0LeuXaa: 0.0 ± 0.0
Met
1.917MetAla: 1.917 ± 0.041
0.106MetCys: 0.106 ± 0.012
0.847MetAsp: 0.847 ± 0.028
0.528MetGlu: 0.528 ± 0.027
0.464MetPhe: 0.464 ± 0.022
1.049MetGly: 1.049 ± 0.039
0.317MetHis: 0.317 ± 0.017
0.572MetIle: 0.572 ± 0.028
0.244MetLys: 0.244 ± 0.016
1.609MetLeu: 1.609 ± 0.041
0.226MetMet: 0.226 ± 0.016
0.338MetAsn: 0.338 ± 0.018
1.046MetPro: 1.046 ± 0.033
0.372MetGln: 0.372 ± 0.02
1.173MetArg: 1.173 ± 0.037
1.367MetSer: 1.367 ± 0.035
1.58MetThr: 1.58 ± 0.041
1.272MetVal: 1.272 ± 0.037
0.161MetTrp: 0.161 ± 0.012
0.254MetTyr: 0.254 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
1.777AsnAla: 1.777 ± 0.045
0.083AsnCys: 0.083 ± 0.011
0.938AsnAsp: 0.938 ± 0.033
0.7AsnGlu: 0.7 ± 0.028
0.45AsnPhe: 0.45 ± 0.024
1.445AsnGly: 1.445 ± 0.049
0.341AsnHis: 0.341 ± 0.017
0.518AsnIle: 0.518 ± 0.026
0.275AsnLys: 0.275 ± 0.016
1.52AsnLeu: 1.52 ± 0.039
0.223AsnMet: 0.223 ± 0.016
0.306AsnAsn: 0.306 ± 0.02
1.136AsnPro: 1.136 ± 0.032
0.437AsnGln: 0.437 ± 0.023
0.971AsnArg: 0.971 ± 0.032
0.601AsnSer: 0.601 ± 0.028
0.824AsnThr: 0.824 ± 0.035
1.474AsnVal: 1.474 ± 0.04
0.182AsnTrp: 0.182 ± 0.013
0.324AsnTyr: 0.324 ± 0.017
0.0AsnXaa: 0.0 ± 0.0
Pro
9.078ProAla: 9.078 ± 0.126
0.225ProCys: 0.225 ± 0.016
4.77ProAsp: 4.77 ± 0.08
3.808ProGlu: 3.808 ± 0.071
1.488ProPhe: 1.488 ± 0.042
6.856ProGly: 6.856 ± 0.1
1.132ProHis: 1.132 ± 0.039
1.168ProIle: 1.168 ± 0.044
0.78ProLys: 0.78 ± 0.032
4.731ProLeu: 4.731 ± 0.077
0.834ProMet: 0.834 ± 0.036
0.74ProAsn: 0.74 ± 0.03
3.005ProPro: 3.005 ± 0.086
1.563ProGln: 1.563 ± 0.043
4.126ProArg: 4.126 ± 0.078
3.344ProSer: 3.344 ± 0.067
3.591ProThr: 3.591 ± 0.068
5.979ProVal: 5.979 ± 0.086
0.894ProTrp: 0.894 ± 0.033
1.089ProTyr: 1.089 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
3.261GlnAla: 3.261 ± 0.07
0.127GlnCys: 0.127 ± 0.014
1.642GlnAsp: 1.642 ± 0.041
1.268GlnGlu: 1.268 ± 0.037
0.573GlnPhe: 0.573 ± 0.026
2.25GlnGly: 2.25 ± 0.044
0.604GlnHis: 0.604 ± 0.027
1.06GlnIle: 1.06 ± 0.037
0.501GlnLys: 0.501 ± 0.027
2.371GlnLeu: 2.371 ± 0.056
0.45GlnMet: 0.45 ± 0.021
0.469GlnAsn: 0.469 ± 0.024
1.353GlnPro: 1.353 ± 0.039
0.949GlnGln: 0.949 ± 0.038
2.087GlnArg: 2.087 ± 0.048
1.142GlnSer: 1.142 ± 0.034
1.377GlnThr: 1.377 ± 0.039
2.894GlnVal: 2.894 ± 0.053
0.381GlnTrp: 0.381 ± 0.02
0.533GlnTyr: 0.533 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
10.997ArgAla: 10.997 ± 0.145
0.435ArgCys: 0.435 ± 0.022
4.593ArgAsp: 4.593 ± 0.066
4.382ArgGlu: 4.382 ± 0.079
2.123ArgPhe: 2.123 ± 0.048
6.417ArgGly: 6.417 ± 0.095
1.633ArgHis: 1.633 ± 0.043
2.466ArgIle: 2.466 ± 0.057
1.085ArgLys: 1.085 ± 0.037
7.899ArgLeu: 7.899 ± 0.099
1.556ArgMet: 1.556 ± 0.039
1.009ArgAsn: 1.009 ± 0.035
4.66ArgPro: 4.66 ± 0.078
1.756ArgGln: 1.756 ± 0.04
7.976ArgArg: 7.976 ± 0.116
4.291ArgSer: 4.291 ± 0.069
5.356ArgThr: 5.356 ± 0.078
6.787ArgVal: 6.787 ± 0.091
1.518ArgTrp: 1.518 ± 0.041
1.613ArgTyr: 1.613 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
7.251SerAla: 7.251 ± 0.1
0.303SerCys: 0.303 ± 0.019
3.185SerAsp: 3.185 ± 0.074
2.192SerGlu: 2.192 ± 0.048
1.578SerPhe: 1.578 ± 0.045
6.071SerGly: 6.071 ± 0.081
0.857SerHis: 0.857 ± 0.029
1.608SerIle: 1.608 ± 0.04
0.785SerLys: 0.785 ± 0.03
4.678SerLeu: 4.678 ± 0.059
1.033SerMet: 1.033 ± 0.037
0.824SerAsn: 0.824 ± 0.034
3.088SerPro: 3.088 ± 0.054
1.208SerGln: 1.208 ± 0.04
3.675SerArg: 3.675 ± 0.058
3.406SerSer: 3.406 ± 0.09
3.742SerThr: 3.742 ± 0.073
4.725SerVal: 4.725 ± 0.089
0.887SerTrp: 0.887 ± 0.031
1.102SerTyr: 1.102 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
8.216ThrAla: 8.216 ± 0.111
0.365ThrCys: 0.365 ± 0.023
3.937ThrAsp: 3.937 ± 0.073
2.891ThrGlu: 2.891 ± 0.061
1.89ThrPhe: 1.89 ± 0.045
6.427ThrGly: 6.427 ± 0.089
1.106ThrHis: 1.106 ± 0.031
2.045ThrIle: 2.045 ± 0.049
1.026ThrLys: 1.026 ± 0.038
5.791ThrLeu: 5.791 ± 0.074
0.908ThrMet: 0.908 ± 0.029
1.002ThrAsn: 1.002 ± 0.041
4.072ThrPro: 4.072 ± 0.073
1.429ThrGln: 1.429 ± 0.04
3.933ThrArg: 3.933 ± 0.064
3.853ThrSer: 3.853 ± 0.075
4.555ThrThr: 4.555 ± 0.103
6.631ThrVal: 6.631 ± 0.105
0.983ThrTrp: 0.983 ± 0.036
1.364ThrTyr: 1.364 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
14.785ValAla: 14.785 ± 0.138
0.635ValCys: 0.635 ± 0.025
6.942ValAsp: 6.942 ± 0.083
5.48ValGlu: 5.48 ± 0.096
2.239ValPhe: 2.239 ± 0.055
8.792ValGly: 8.792 ± 0.116
2.014ValHis: 2.014 ± 0.047
2.454ValIle: 2.454 ± 0.067
1.281ValLys: 1.281 ± 0.04
11.109ValLeu: 11.109 ± 0.135
1.331ValMet: 1.331 ± 0.04
1.418ValAsn: 1.418 ± 0.039
6.252ValPro: 6.252 ± 0.086
2.183ValGln: 2.183 ± 0.043
8.178ValArg: 8.178 ± 0.116
4.872ValSer: 4.872 ± 0.078
6.347ValThr: 6.347 ± 0.105
12.703ValVal: 12.703 ± 0.163
1.309ValTrp: 1.309 ± 0.039
1.577ValTyr: 1.577 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
1.707TrpAla: 1.707 ± 0.044
0.131TrpCys: 0.131 ± 0.012
0.926TrpAsp: 0.926 ± 0.034
0.58TrpGlu: 0.58 ± 0.025
0.529TrpPhe: 0.529 ± 0.026
1.114TrpGly: 1.114 ± 0.039
0.331TrpHis: 0.331 ± 0.021
0.482TrpIle: 0.482 ± 0.022
0.258TrpLys: 0.258 ± 0.018
1.623TrpLeu: 1.623 ± 0.04
0.256TrpMet: 0.256 ± 0.015
0.336TrpAsn: 0.336 ± 0.022
0.73TrpPro: 0.73 ± 0.031
0.476TrpGln: 0.476 ± 0.022
1.262TrpArg: 1.262 ± 0.038
1.022TrpSer: 1.022 ± 0.033
1.045TrpThr: 1.045 ± 0.033
1.302TrpVal: 1.302 ± 0.035
0.395TrpTrp: 0.395 ± 0.021
0.308TrpTyr: 0.308 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.46TyrAla: 2.46 ± 0.05
0.116TyrCys: 0.116 ± 0.01
1.367TyrAsp: 1.367 ± 0.046
0.986TyrGlu: 0.986 ± 0.032
0.545TyrPhe: 0.545 ± 0.022
1.942TyrGly: 1.942 ± 0.052
0.296TyrHis: 0.296 ± 0.021
0.345TyrIle: 0.345 ± 0.019
0.277TyrLys: 0.277 ± 0.018
1.979TyrLeu: 1.979 ± 0.05
0.173TyrMet: 0.173 ± 0.015
0.313TyrAsn: 0.313 ± 0.02
0.991TyrPro: 0.991 ± 0.035
0.526TyrGln: 0.526 ± 0.024
1.56TyrArg: 1.56 ± 0.041
0.945TyrSer: 0.945 ± 0.035
1.088TyrThr: 1.088 ± 0.035
1.845TyrVal: 1.845 ± 0.043
0.282TyrTrp: 0.282 ± 0.017
0.425TyrTyr: 0.425 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2804 proteins (979563 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski