Amino acid dipepetide frequency for Gordonia phage Madeline

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.654AlaAla: 18.654 ± 2.516
0.708AlaCys: 0.708 ± 0.207
8.973AlaAsp: 8.973 ± 0.836
7.674AlaGlu: 7.674 ± 0.845
3.07AlaPhe: 3.07 ± 0.38
8.855AlaGly: 8.855 ± 0.768
2.007AlaHis: 2.007 ± 0.323
4.959AlaIle: 4.959 ± 0.543
4.191AlaLys: 4.191 ± 0.541
8.323AlaLeu: 8.323 ± 0.603
3.07AlaMet: 3.07 ± 0.487
2.656AlaAsn: 2.656 ± 0.418
5.195AlaPro: 5.195 ± 0.428
4.9AlaGln: 4.9 ± 0.731
7.615AlaArg: 7.615 ± 0.649
6.08AlaSer: 6.08 ± 0.834
8.56AlaThr: 8.56 ± 1.267
8.796AlaVal: 8.796 ± 0.955
1.83AlaTrp: 1.83 ± 0.336
2.42AlaTyr: 2.42 ± 0.303
0.0AlaXaa: 0.0 ± 0.0
Cys
0.649CysAla: 0.649 ± 0.201
0.0CysCys: 0.0 ± 0.0
0.472CysAsp: 0.472 ± 0.198
0.413CysGlu: 0.413 ± 0.159
0.059CysPhe: 0.059 ± 0.057
1.004CysGly: 1.004 ± 0.319
0.118CysHis: 0.118 ± 0.094
0.059CysIle: 0.059 ± 0.06
0.236CysLys: 0.236 ± 0.117
0.472CysLeu: 0.472 ± 0.178
0.236CysMet: 0.236 ± 0.122
0.354CysAsn: 0.354 ± 0.156
0.531CysPro: 0.531 ± 0.204
0.177CysGln: 0.177 ± 0.112
0.767CysArg: 0.767 ± 0.247
0.236CysSer: 0.236 ± 0.115
0.472CysThr: 0.472 ± 0.239
0.295CysVal: 0.295 ± 0.126
0.118CysTrp: 0.118 ± 0.075
0.295CysTyr: 0.295 ± 0.14
0.0CysXaa: 0.0 ± 0.0
Asp
7.438AspAla: 7.438 ± 0.76
0.649AspCys: 0.649 ± 0.27
6.494AspAsp: 6.494 ± 0.923
4.723AspGlu: 4.723 ± 0.605
1.535AspPhe: 1.535 ± 0.34
5.844AspGly: 5.844 ± 0.822
2.007AspHis: 2.007 ± 0.386
2.066AspIle: 2.066 ± 0.312
1.771AspLys: 1.771 ± 0.279
7.379AspLeu: 7.379 ± 0.619
1.004AspMet: 1.004 ± 0.279
2.42AspAsn: 2.42 ± 0.385
5.726AspPro: 5.726 ± 0.547
1.771AspGln: 1.771 ± 0.325
4.959AspArg: 4.959 ± 0.688
3.07AspSer: 3.07 ± 0.543
3.955AspThr: 3.955 ± 0.545
5.254AspVal: 5.254 ± 0.527
1.476AspTrp: 1.476 ± 0.235
1.122AspTyr: 1.122 ± 0.194
0.0AspXaa: 0.0 ± 0.0
Glu
6.553GluAla: 6.553 ± 0.766
0.472GluCys: 0.472 ± 0.18
3.365GluAsp: 3.365 ± 0.54
3.424GluGlu: 3.424 ± 0.428
1.948GluPhe: 1.948 ± 0.344
3.011GluGly: 3.011 ± 0.533
0.945GluHis: 0.945 ± 0.24
3.424GluIle: 3.424 ± 0.443
2.538GluLys: 2.538 ± 0.39
5.431GluLeu: 5.431 ± 0.709
0.767GluMet: 0.767 ± 0.184
1.417GluAsn: 1.417 ± 0.346
2.479GluPro: 2.479 ± 0.414
2.834GluGln: 2.834 ± 0.405
5.372GluArg: 5.372 ± 0.862
2.656GluSer: 2.656 ± 0.348
3.247GluThr: 3.247 ± 0.399
4.25GluVal: 4.25 ± 0.449
1.004GluTrp: 1.004 ± 0.256
1.24GluTyr: 1.24 ± 0.236
0.0GluXaa: 0.0 ± 0.0
Phe
3.07PheAla: 3.07 ± 0.397
0.118PheCys: 0.118 ± 0.092
2.656PheAsp: 2.656 ± 0.352
1.771PheGlu: 1.771 ± 0.333
0.59PhePhe: 0.59 ± 0.174
2.538PheGly: 2.538 ± 0.428
0.472PheHis: 0.472 ± 0.174
1.063PheIle: 1.063 ± 0.262
0.885PheLys: 0.885 ± 0.257
0.767PheLeu: 0.767 ± 0.268
0.531PheMet: 0.531 ± 0.153
0.885PheAsn: 0.885 ± 0.229
1.417PhePro: 1.417 ± 0.295
0.472PheGln: 0.472 ± 0.14
2.243PheArg: 2.243 ± 0.488
1.476PheSer: 1.476 ± 0.325
1.476PheThr: 1.476 ± 0.293
1.653PheVal: 1.653 ± 0.321
0.945PheTrp: 0.945 ± 0.213
0.708PheTyr: 0.708 ± 0.219
0.0PheXaa: 0.0 ± 0.0
Gly
7.969GlyAla: 7.969 ± 0.671
0.295GlyCys: 0.295 ± 0.152
5.254GlyAsp: 5.254 ± 0.453
4.132GlyGlu: 4.132 ± 0.554
2.066GlyPhe: 2.066 ± 0.358
8.442GlyGly: 8.442 ± 1.232
1.476GlyHis: 1.476 ± 0.31
3.955GlyIle: 3.955 ± 0.388
3.306GlyLys: 3.306 ± 0.416
6.966GlyLeu: 6.966 ± 0.643
2.125GlyMet: 2.125 ± 0.365
3.542GlyAsn: 3.542 ± 0.49
4.073GlyPro: 4.073 ± 0.439
2.893GlyGln: 2.893 ± 0.405
6.08GlyArg: 6.08 ± 0.592
5.195GlySer: 5.195 ± 0.613
6.139GlyThr: 6.139 ± 1.113
5.726GlyVal: 5.726 ± 0.648
1.417GlyTrp: 1.417 ± 0.336
2.125GlyTyr: 2.125 ± 0.296
0.0GlyXaa: 0.0 ± 0.0
His
2.361HisAla: 2.361 ± 0.323
0.177HisCys: 0.177 ± 0.113
1.122HisAsp: 1.122 ± 0.26
0.885HisGlu: 0.885 ± 0.239
0.295HisPhe: 0.295 ± 0.138
1.358HisGly: 1.358 ± 0.268
0.708HisHis: 0.708 ± 0.193
0.826HisIle: 0.826 ± 0.266
0.472HisLys: 0.472 ± 0.17
2.007HisLeu: 2.007 ± 0.4
0.236HisMet: 0.236 ± 0.119
0.295HisAsn: 0.295 ± 0.117
1.535HisPro: 1.535 ± 0.323
0.767HisGln: 0.767 ± 0.246
1.653HisArg: 1.653 ± 0.376
1.122HisSer: 1.122 ± 0.281
1.712HisThr: 1.712 ± 0.28
1.181HisVal: 1.181 ± 0.31
0.531HisTrp: 0.531 ± 0.177
0.826HisTyr: 0.826 ± 0.253
0.0HisXaa: 0.0 ± 0.0
Ile
5.136IleAla: 5.136 ± 0.602
0.354IleCys: 0.354 ± 0.167
4.664IleAsp: 4.664 ± 0.445
3.483IleGlu: 3.483 ± 0.385
0.531IlePhe: 0.531 ± 0.137
4.073IleGly: 4.073 ± 0.499
1.181IleHis: 1.181 ± 0.284
1.535IleIle: 1.535 ± 0.349
1.181IleLys: 1.181 ± 0.248
2.184IleLeu: 2.184 ± 0.361
0.295IleMet: 0.295 ± 0.107
1.417IleAsn: 1.417 ± 0.249
2.656IlePro: 2.656 ± 0.381
2.007IleGln: 2.007 ± 0.361
4.132IleArg: 4.132 ± 0.522
2.361IleSer: 2.361 ± 0.379
2.184IleThr: 2.184 ± 0.354
4.014IleVal: 4.014 ± 0.436
0.354IleTrp: 0.354 ± 0.128
1.063IleTyr: 1.063 ± 0.187
0.0IleXaa: 0.0 ± 0.0
Lys
3.719LysAla: 3.719 ± 0.701
0.0LysCys: 0.0 ± 0.0
1.299LysAsp: 1.299 ± 0.296
1.476LysGlu: 1.476 ± 0.334
1.181LysPhe: 1.181 ± 0.26
2.361LysGly: 2.361 ± 0.403
0.885LysHis: 0.885 ± 0.211
1.535LysIle: 1.535 ± 0.313
1.122LysLys: 1.122 ± 0.292
2.893LysLeu: 2.893 ± 0.483
0.59LysMet: 0.59 ± 0.235
0.885LysAsn: 0.885 ± 0.214
2.715LysPro: 2.715 ± 0.335
0.885LysGln: 0.885 ± 0.304
2.42LysArg: 2.42 ± 0.434
2.656LysSer: 2.656 ± 0.425
2.774LysThr: 2.774 ± 0.472
2.066LysVal: 2.066 ± 0.282
0.708LysTrp: 0.708 ± 0.243
0.708LysTyr: 0.708 ± 0.188
0.0LysXaa: 0.0 ± 0.0
Leu
10.862LeuAla: 10.862 ± 0.916
0.413LeuCys: 0.413 ± 0.183
5.49LeuAsp: 5.49 ± 0.658
4.25LeuGlu: 4.25 ± 0.65
2.834LeuPhe: 2.834 ± 0.597
5.49LeuGly: 5.49 ± 0.379
1.594LeuHis: 1.594 ± 0.491
3.542LeuIle: 3.542 ± 0.452
2.243LeuLys: 2.243 ± 0.397
4.959LeuLeu: 4.959 ± 0.508
1.653LeuMet: 1.653 ± 0.34
2.243LeuAsn: 2.243 ± 0.418
4.545LeuPro: 4.545 ± 0.498
2.834LeuGln: 2.834 ± 0.347
6.198LeuArg: 6.198 ± 0.621
4.191LeuSer: 4.191 ± 0.395
5.962LeuThr: 5.962 ± 0.674
5.195LeuVal: 5.195 ± 0.628
1.712LeuTrp: 1.712 ± 0.337
1.24LeuTyr: 1.24 ± 0.324
0.0LeuXaa: 0.0 ± 0.0
Met
2.184MetAla: 2.184 ± 0.546
0.295MetCys: 0.295 ± 0.138
0.649MetAsp: 0.649 ± 0.203
0.649MetGlu: 0.649 ± 0.19
0.236MetPhe: 0.236 ± 0.119
1.948MetGly: 1.948 ± 0.447
0.472MetHis: 0.472 ± 0.138
0.826MetIle: 0.826 ± 0.249
0.945MetLys: 0.945 ± 0.271
1.83MetLeu: 1.83 ± 0.346
0.236MetMet: 0.236 ± 0.141
0.531MetAsn: 0.531 ± 0.214
1.122MetPro: 1.122 ± 0.257
1.063MetGln: 1.063 ± 0.225
2.007MetArg: 2.007 ± 0.415
1.712MetSer: 1.712 ± 0.316
2.479MetThr: 2.479 ± 0.373
1.181MetVal: 1.181 ± 0.29
0.59MetTrp: 0.59 ± 0.192
0.236MetTyr: 0.236 ± 0.126
0.0MetXaa: 0.0 ± 0.0
Asn
3.306AsnAla: 3.306 ± 0.425
0.236AsnCys: 0.236 ± 0.131
2.243AsnAsp: 2.243 ± 0.343
1.122AsnGlu: 1.122 ± 0.288
0.531AsnPhe: 0.531 ± 0.177
2.715AsnGly: 2.715 ± 0.407
0.767AsnHis: 0.767 ± 0.316
1.122AsnIle: 1.122 ± 0.264
0.649AsnLys: 0.649 ± 0.199
1.594AsnLeu: 1.594 ± 0.311
0.472AsnMet: 0.472 ± 0.149
0.826AsnAsn: 0.826 ± 0.24
2.007AsnPro: 2.007 ± 0.286
1.063AsnGln: 1.063 ± 0.249
2.715AsnArg: 2.715 ± 0.357
1.948AsnSer: 1.948 ± 0.344
2.538AsnThr: 2.538 ± 0.511
1.771AsnVal: 1.771 ± 0.366
0.531AsnTrp: 0.531 ± 0.171
0.885AsnTyr: 0.885 ± 0.261
0.0AsnXaa: 0.0 ± 0.0
Pro
6.671ProAla: 6.671 ± 0.618
0.472ProCys: 0.472 ± 0.2
5.018ProAsp: 5.018 ± 0.847
3.955ProGlu: 3.955 ± 0.581
1.535ProPhe: 1.535 ± 0.281
6.434ProGly: 6.434 ± 0.631
0.531ProHis: 0.531 ± 0.176
2.597ProIle: 2.597 ± 0.314
2.066ProLys: 2.066 ± 0.422
3.837ProLeu: 3.837 ± 0.455
1.535ProMet: 1.535 ± 0.257
1.83ProAsn: 1.83 ± 0.293
2.774ProPro: 2.774 ± 0.427
1.712ProGln: 1.712 ± 0.338
3.778ProArg: 3.778 ± 0.536
3.188ProSer: 3.188 ± 0.422
3.955ProThr: 3.955 ± 0.545
4.132ProVal: 4.132 ± 0.465
1.417ProTrp: 1.417 ± 0.261
0.767ProTyr: 0.767 ± 0.178
0.0ProXaa: 0.0 ± 0.0
Gln
3.955GlnAla: 3.955 ± 0.536
0.295GlnCys: 0.295 ± 0.15
1.358GlnAsp: 1.358 ± 0.258
1.299GlnGlu: 1.299 ± 0.246
0.767GlnPhe: 0.767 ± 0.193
2.834GlnGly: 2.834 ± 0.677
0.826GlnHis: 0.826 ± 0.215
2.42GlnIle: 2.42 ± 0.422
1.24GlnLys: 1.24 ± 0.377
3.778GlnLeu: 3.778 ± 0.484
1.181GlnMet: 1.181 ± 0.294
1.063GlnAsn: 1.063 ± 0.262
2.184GlnPro: 2.184 ± 0.321
1.653GlnGln: 1.653 ± 0.319
3.424GlnArg: 3.424 ± 0.514
1.712GlnSer: 1.712 ± 0.409
1.889GlnThr: 1.889 ± 0.41
2.715GlnVal: 2.715 ± 0.507
0.826GlnTrp: 0.826 ± 0.224
0.59GlnTyr: 0.59 ± 0.171
0.0GlnXaa: 0.0 ± 0.0
Arg
7.497ArgAla: 7.497 ± 0.583
0.59ArgCys: 0.59 ± 0.248
5.431ArgAsp: 5.431 ± 0.61
4.9ArgGlu: 4.9 ± 0.544
2.125ArgPhe: 2.125 ± 0.36
5.018ArgGly: 5.018 ± 0.519
1.417ArgHis: 1.417 ± 0.358
3.542ArgIle: 3.542 ± 0.563
2.952ArgLys: 2.952 ± 0.354
6.789ArgLeu: 6.789 ± 0.543
2.361ArgMet: 2.361 ± 0.369
2.125ArgAsn: 2.125 ± 0.389
5.136ArgPro: 5.136 ± 0.784
2.893ArgGln: 2.893 ± 0.547
5.903ArgArg: 5.903 ± 0.871
4.073ArgSer: 4.073 ± 0.494
5.785ArgThr: 5.785 ± 0.648
5.018ArgVal: 5.018 ± 0.655
2.007ArgTrp: 2.007 ± 0.39
1.83ArgTyr: 1.83 ± 0.346
0.0ArgXaa: 0.0 ± 0.0
Ser
5.962SerAla: 5.962 ± 0.924
0.413SerCys: 0.413 ± 0.237
4.309SerAsp: 4.309 ± 0.519
3.011SerGlu: 3.011 ± 0.399
1.299SerPhe: 1.299 ± 0.257
5.313SerGly: 5.313 ± 0.665
0.649SerHis: 0.649 ± 0.209
2.952SerIle: 2.952 ± 0.548
2.42SerLys: 2.42 ± 0.324
3.955SerLeu: 3.955 ± 0.537
1.476SerMet: 1.476 ± 0.394
1.889SerAsn: 1.889 ± 0.293
2.597SerPro: 2.597 ± 0.413
2.184SerGln: 2.184 ± 0.4
4.014SerArg: 4.014 ± 0.574
2.774SerSer: 2.774 ± 0.46
4.486SerThr: 4.486 ± 0.662
4.368SerVal: 4.368 ± 0.454
1.299SerTrp: 1.299 ± 0.265
1.24SerTyr: 1.24 ± 0.323
0.0SerXaa: 0.0 ± 0.0
Thr
10.272ThrAla: 10.272 ± 1.513
0.531ThrCys: 0.531 ± 0.201
4.782ThrAsp: 4.782 ± 0.538
3.778ThrGlu: 3.778 ± 0.464
1.476ThrPhe: 1.476 ± 0.269
6.375ThrGly: 6.375 ± 0.749
1.122ThrHis: 1.122 ± 0.264
3.719ThrIle: 3.719 ± 0.497
1.771ThrLys: 1.771 ± 0.337
5.136ThrLeu: 5.136 ± 0.472
1.476ThrMet: 1.476 ± 0.364
1.653ThrAsn: 1.653 ± 0.327
4.545ThrPro: 4.545 ± 0.544
1.653ThrGln: 1.653 ± 0.287
4.841ThrArg: 4.841 ± 0.555
5.313ThrSer: 5.313 ± 0.784
5.962ThrThr: 5.962 ± 0.813
5.726ThrVal: 5.726 ± 0.6
0.945ThrTrp: 0.945 ± 0.254
1.712ThrTyr: 1.712 ± 0.363
0.0ThrXaa: 0.0 ± 0.0
Val
7.615ValAla: 7.615 ± 0.848
0.59ValCys: 0.59 ± 0.189
5.018ValAsp: 5.018 ± 0.619
3.896ValGlu: 3.896 ± 0.478
2.715ValPhe: 2.715 ± 0.447
5.726ValGly: 5.726 ± 0.649
1.653ValHis: 1.653 ± 0.26
3.66ValIle: 3.66 ± 0.395
1.594ValLys: 1.594 ± 0.334
6.08ValLeu: 6.08 ± 0.672
0.945ValMet: 0.945 ± 0.186
2.184ValAsn: 2.184 ± 0.392
4.545ValPro: 4.545 ± 0.518
2.538ValGln: 2.538 ± 0.672
5.431ValArg: 5.431 ± 0.619
4.014ValSer: 4.014 ± 0.588
6.316ValThr: 6.316 ± 0.633
5.018ValVal: 5.018 ± 0.54
0.649ValTrp: 0.649 ± 0.18
1.122ValTyr: 1.122 ± 0.195
0.0ValXaa: 0.0 ± 0.0
Trp
1.771TrpAla: 1.771 ± 0.262
0.118TrpCys: 0.118 ± 0.079
0.885TrpAsp: 0.885 ± 0.257
0.945TrpGlu: 0.945 ± 0.243
0.472TrpPhe: 0.472 ± 0.158
1.417TrpGly: 1.417 ± 0.308
0.649TrpHis: 0.649 ± 0.235
0.413TrpIle: 0.413 ± 0.154
0.472TrpLys: 0.472 ± 0.163
1.653TrpLeu: 1.653 ± 0.349
0.531TrpMet: 0.531 ± 0.189
0.354TrpAsn: 0.354 ± 0.128
1.063TrpPro: 1.063 ± 0.207
0.708TrpGln: 0.708 ± 0.239
2.243TrpArg: 2.243 ± 0.42
1.122TrpSer: 1.122 ± 0.266
1.594TrpThr: 1.594 ± 0.302
1.594TrpVal: 1.594 ± 0.304
0.472TrpTrp: 0.472 ± 0.163
0.649TrpTyr: 0.649 ± 0.166
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.952TyrAla: 2.952 ± 0.352
0.236TyrCys: 0.236 ± 0.115
1.181TyrAsp: 1.181 ± 0.261
0.59TyrGlu: 0.59 ± 0.185
0.472TyrPhe: 0.472 ± 0.12
2.42TyrGly: 2.42 ± 0.293
0.59TyrHis: 0.59 ± 0.237
0.59TyrIle: 0.59 ± 0.177
0.826TyrLys: 0.826 ± 0.236
1.417TyrLeu: 1.417 ± 0.317
0.413TyrMet: 0.413 ± 0.147
0.472TyrAsn: 0.472 ± 0.163
1.358TyrPro: 1.358 ± 0.307
1.004TyrGln: 1.004 ± 0.222
1.594TyrArg: 1.594 ± 0.37
1.712TyrSer: 1.712 ± 0.277
1.299TyrThr: 1.299 ± 0.225
1.358TyrVal: 1.358 ± 0.291
0.295TyrTrp: 0.295 ± 0.119
0.885TyrTyr: 0.885 ± 0.216
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (16941 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski