Chytriomyces confervae
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10661 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A507FKB3|A0A507FKB3_9FUNG NTP_transf_2 domain-containing protein OS=Chytriomyces confervae OX=246404 GN=CcCBS67573_g02879 PE=4 SV=1
MM1 pKa = 7.28 FATSANLDD9 pKa = 3.87 TRR11 pKa = 11.84 PSAPDD16 pKa = 3.34 SPSEE20 pKa = 3.86 QASVKK25 pKa = 9.84 TSFGSYY31 pKa = 9.73 IIRR34 pKa = 11.84 DD35 pKa = 3.6 VRR37 pKa = 11.84 FVIATACDD45 pKa = 3.59 ISPGPSTKK53 pKa = 10.1 NRR55 pKa = 11.84 LILPKK60 pKa = 9.21 PASVFYY66 pKa = 11.19 SPITTCITLYY76 pKa = 9.66 GTTHH80 pKa = 6.61 PRR82 pKa = 11.84 SKK84 pKa = 11.1 NLLLEE89 pKa = 4.3 MIFNKK94 pKa = 9.92 IVATAILFAYY104 pKa = 10.32 ASIAFPANIAPPSEE118 pKa = 4.28 VAPGGAGTSIPSAEE132 pKa = 4.18 PQEE135 pKa = 4.04 ITPTSFAPVPSPLLDD150 pKa = 3.35 TAFPPVANDD159 pKa = 3.22 INGLCGGGSAFKK171 pKa = 10.99 NPLACKK177 pKa = 9.86 PGLVCAKK184 pKa = 10.12 QVGSSDD190 pKa = 3.79 DD191 pKa = 3.63 TLGTCQIPNVSDD203 pKa = 3.25 VGGSCGGEE211 pKa = 3.96 VFNAATCAVGLVCISDD227 pKa = 3.84 AADD230 pKa = 3.11 ATAPGTCQIVVATPTPTSEE249 pKa = 3.92 PVGLGGLVTGTEE261 pKa = 4.2 EE262 pKa = 4.14 VVATGGIVGTEE273 pKa = 3.82 PVGTPSEE280 pKa = 4.42 TVAGGGIVGTEE291 pKa = 3.91 LVEE294 pKa = 3.89 PTIAATSEE302 pKa = 4.38 TTVSAPVPSSTTTTSKK318 pKa = 7.98 TTTTSTASSIVSTVFGEE335 pKa = 4.27 NFPPALTSFTTTSEE349 pKa = 3.86 EE350 pKa = 3.98 LAPPSPSNGAASVLPTLGTEE370 pKa = 4.08 PAEE373 pKa = 4.36 PPTPSVAPAGTEE385 pKa = 3.91 PVTEE389 pKa = 4.43 TPSPAGPSTPTEE401 pKa = 4.25 APASSTTSEE410 pKa = 4.3 EE411 pKa = 4.28 APPTPSNPAAGTQPVPEE428 pKa = 4.74 PSSTSATTATSEE440 pKa = 4.63 EE441 pKa = 4.34 IPPTPSIGEE450 pKa = 4.28 AEE452 pKa = 4.3 TEE454 pKa = 4.22 TLDD457 pKa = 3.81 EE458 pKa = 4.34 LTISPLTGNPPTLPTTTTSEE478 pKa = 4.2 GAPPSPSVPAAEE490 pKa = 4.31 TEE492 pKa = 4.42 PVSSADD498 pKa = 3.97 KK499 pKa = 10.8 ISTTTDD505 pKa = 2.81 TTFIEE510 pKa = 4.73 VPPSLSVPAAEE521 pKa = 4.65 TEE523 pKa = 4.32 PVPPTDD529 pKa = 3.47 SSSATSSEE537 pKa = 3.83 EE538 pKa = 3.83 TTTVIEE544 pKa = 4.45 VPPSPSVPAAEE555 pKa = 4.46 TEE557 pKa = 4.27 PVPPADD563 pKa = 3.65 TSSAATTADD572 pKa = 3.67 KK573 pKa = 10.65 PPQEE577 pKa = 4.4 SSSVTDD583 pKa = 3.2 SSAPAEE589 pKa = 4.4 PNSSEE594 pKa = 4.27 STSDD598 pKa = 2.79 TTAAVAEE605 pKa = 4.66 PEE607 pKa = 4.49 TTTQAPTSAAEE618 pKa = 3.77 ITTAAEE624 pKa = 4.11 ATTTAAEE631 pKa = 4.26 IVSSEE636 pKa = 4.28 EE637 pKa = 3.89 PSTTTSDD644 pKa = 3.21 SAIFIPEE651 pKa = 4.2 GPTEE655 pKa = 4.01 EE656 pKa = 4.75 SSTSVEE662 pKa = 4.11 TTTASDD668 pKa = 5.43 DD669 pKa = 3.91 IPPSPSDD676 pKa = 3.39 GAGDD680 pKa = 3.4 IVSTTTAAAIDD691 pKa = 3.88 VSLEE695 pKa = 3.99 TSEE698 pKa = 4.88 SQVAPSPSTSEE709 pKa = 4.05 EE710 pKa = 4.41 VPTTTTTTSVEE721 pKa = 4.35 SSVTTADD728 pKa = 3.2 AGPRR732 pKa = 11.84 ALTLPEE738 pKa = 4.68 AYY740 pKa = 10.41 SLVTRR745 pKa = 11.84 EE746 pKa = 4.24 LSSLPSCLIGCLNPDD761 pKa = 3.35 HH762 pKa = 7.05 SSSVTDD768 pKa = 3.49 DD769 pKa = 3.56 TANDD773 pKa = 3.68 LCIDD777 pKa = 4.03 AFGNDD782 pKa = 3.37 PFALVMCIVSDD793 pKa = 3.92 CTGGDD798 pKa = 3.28 FDD800 pKa = 5.01 VVINALTDD808 pKa = 3.48 PTITEE813 pKa = 4.37 GLSNACNVIYY823 pKa = 10.58 ASIPEE828 pKa = 4.41 TISTSFEE835 pKa = 3.92 SSSAAEE841 pKa = 4.06 PTTTTTAASVAAAPVSPIFSSSTWSMFAAAPVKK874 pKa = 10.69 NGVNGTCGGYY884 pKa = 10.39 AADD887 pKa = 5.52 AATCLPGLKK896 pKa = 9.88 CVSKK900 pKa = 10.81 SVNEE904 pKa = 4.05 VGTCQKK910 pKa = 10.56 PVVNDD915 pKa = 3.24 VGQTCGGSITNNPASCAGSLACMPNFIPGLPGTCQMPVEE954 pKa = 4.5 PSPLTSLSSSSIVVRR969 pKa = 11.84 PTTTKK974 pKa = 9.45 KK975 pKa = 8.64 HH976 pKa = 5.32 KK977 pKa = 9.68 KK978 pKa = 7.92 YY979 pKa = 10.69 RR980 pKa = 11.84 MVIKK984 pKa = 9.47 TVEE987 pKa = 4.3 CTTTTVTTTTSTQYY1001 pKa = 11.12 AIQSSSPP1008 pKa = 3.22
Molecular weight: 100.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.834
IPC_protein 3.821
Toseland 3.63
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.643
Rodwell 3.643
Grimsley 3.541
Solomon 3.77
Lehninger 3.719
Nozaki 3.872
DTASelect 4.024
Thurlkill 3.643
EMBOSS 3.656
Sillero 3.923
Patrickios 0.909
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.83
Protein with the highest isoelectric point:
>tr|A0A507D400|A0A507D400_9FUNG Pirin domain-containing protein OS=Chytriomyces confervae OX=246404 GN=CcCBS67573_g10330 PE=3 SV=1
MM1 pKa = 7.54 SSARR5 pKa = 11.84 LTGPIPGSFGNMINLVEE22 pKa = 5.81 LRR24 pKa = 11.84 LQMNKK29 pKa = 10.33 LLGTVLVIACNSRR42 pKa = 11.84 PSNAKK47 pKa = 9.24 RR48 pKa = 11.84 INFRR52 pKa = 11.84 RR53 pKa = 11.84 PTTCNASAEE62 pKa = 4.23 RR63 pKa = 11.84 IEE65 pKa = 4.5 MGDD68 pKa = 3.49 NQLTPIVDD76 pKa = 3.24 IDD78 pKa = 3.57 SDD80 pKa = 3.76 RR81 pKa = 11.84 TSGYY85 pKa = 9.48 IYY87 pKa = 9.24 PFPSVSLDD95 pKa = 3.56 LQSPNAGSNQLQEE108 pKa = 5.34 DD109 pKa = 4.4 SFADD113 pKa = 4.37 TIDD116 pKa = 3.56 SDD118 pKa = 4.75 SMFTSRR124 pKa = 11.84 LGLSRR129 pKa = 11.84 TQLSRR134 pKa = 11.84 KK135 pKa = 9.46 LPNCSKK141 pKa = 10.76 KK142 pKa = 10.65 SATHH146 pKa = 5.77 SPQRR150 pKa = 11.84 QQSRR154 pKa = 11.84 WSVSHH159 pKa = 6.63 RR160 pKa = 11.84 NWRR163 pKa = 11.84 LRR165 pKa = 11.84 KK166 pKa = 8.82 IGKK169 pKa = 9.14 RR170 pKa = 11.84 RR171 pKa = 3.3
Molecular weight: 19.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.575
IPC_protein 10.467
Toseland 10.833
ProMoST 10.921
Dawson 10.891
Bjellqvist 10.643
Wikipedia 11.14
Rodwell 11.023
Grimsley 10.921
Solomon 11.082
Lehninger 11.052
Nozaki 10.833
DTASelect 10.628
Thurlkill 10.818
EMBOSS 11.257
Sillero 10.833
Patrickios 10.76
IPC_peptide 11.096
IPC2_peptide 9.838
IPC2.peptide.svr19 8.892
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10661
0
10661
5997939
10
6810
562.6
61.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.948 ± 0.02
1.337 ± 0.009
5.37 ± 0.016
5.826 ± 0.026
4.059 ± 0.014
6.246 ± 0.022
2.304 ± 0.011
5.087 ± 0.016
5.394 ± 0.022
9.224 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.415 ± 0.008
4.235 ± 0.012
5.138 ± 0.023
3.998 ± 0.018
5.055 ± 0.017
8.957 ± 0.032
5.937 ± 0.025
6.8 ± 0.024
1.111 ± 0.007
2.558 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here