Rhododendron williamsianum
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21355 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6A4KF71|A0A6A4KF71_9ERIC Germin-like protein (Fragment) OS=Rhododendron williamsianum OX=262921 GN=C3L33_21356 PE=3 SV=1
MM1 pKa = 7.52 FEE3 pKa = 3.63 QYY5 pKa = 11.2 YY6 pKa = 9.38 YY7 pKa = 10.38 FQAWQDD13 pKa = 3.76 DD14 pKa = 4.02 EE15 pKa = 6.38 SIIPTKK21 pKa = 10.66 PFDD24 pKa = 5.14 DD25 pKa = 5.75 DD26 pKa = 4.28 DD27 pKa = 4.44 HH28 pKa = 8.72 LSYY31 pKa = 10.7 FYY33 pKa = 10.73 IKK35 pKa = 10.62 VSAEE39 pKa = 3.4 LDD41 pKa = 3.64 AEE43 pKa = 4.47 HH44 pKa = 6.51 VTGFWVPFDD53 pKa = 3.82 QLVDD57 pKa = 4.03 DD58 pKa = 5.41 DD59 pKa = 5.51 FSTSRR64 pKa = 11.84 PIVSAILSEE73 pKa = 4.3 VCVPVDD79 pKa = 3.82 AQPDD83 pKa = 3.98 MVDD86 pKa = 4.13 HH87 pKa = 6.88 IVGCARR93 pKa = 11.84 SMARR97 pKa = 11.84 EE98 pKa = 4.09 SCNVGSVVLPMFVHH112 pKa = 6.67 IVSEE116 pKa = 4.82 DD117 pKa = 3.72 YY118 pKa = 11.07 ILDD121 pKa = 4.15 DD122 pKa = 5.22 DD123 pKa = 5.07 VRR125 pKa = 11.84 DD126 pKa = 4.09 DD127 pKa = 3.88 VDD129 pKa = 5.07 DD130 pKa = 4.33 NFAVLEE136 pKa = 4.26 SSNGGAGGSTGG147 pKa = 3.34
Molecular weight: 16.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.681
IPC_protein 3.694
Toseland 3.465
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.376
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 4.101
Thurlkill 3.528
EMBOSS 3.681
Sillero 3.821
Patrickios 0.744
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.745
Protein with the highest isoelectric point:
>tr|A0A6A4KY64|A0A6A4KY64_9ERIC Non-specific serine/threonine protein kinase (Fragment) OS=Rhododendron williamsianum OX=262921 GN=C3L33_17889 PE=4 SV=1
MM1 pKa = 7.63 TSTCISNCINDD12 pKa = 2.9 ARR14 pKa = 11.84 APVRR18 pKa = 11.84 ATYY21 pKa = 10.92 VNLYY25 pKa = 9.28 KK26 pKa = 10.28 WPEE29 pKa = 3.66 SDD31 pKa = 3.72 AEE33 pKa = 4.15 FVRR36 pKa = 11.84 TVSSGGGGDD45 pKa = 3.4 RR46 pKa = 11.84 QPRR49 pKa = 11.84 LHH51 pKa = 6.72 PRR53 pKa = 11.84 VVDD56 pKa = 4.57 SISCRR61 pKa = 11.84 QLYY64 pKa = 9.35 LRR66 pKa = 11.84 SYY68 pKa = 8.01 TFSRR72 pKa = 11.84 KK73 pKa = 6.68 EE74 pKa = 3.96 TVPEE78 pKa = 3.9 RR79 pKa = 11.84 TKK81 pKa = 10.91 KK82 pKa = 9.95 CLARR86 pKa = 11.84 ARR88 pKa = 11.84 EE89 pKa = 3.94 RR90 pKa = 11.84 VARR93 pKa = 11.84 AGRR96 pKa = 11.84 RR97 pKa = 11.84 GKK99 pKa = 10.89 GRR101 pKa = 11.84 VHH103 pKa = 6.68 GKK105 pKa = 9.71 RR106 pKa = 11.84 RR107 pKa = 11.84 VAVAGRR113 pKa = 11.84 RR114 pKa = 11.84 AKK116 pKa = 10.5 DD117 pKa = 3.17 VWCAALCAIFHH128 pKa = 6.98 RR129 pKa = 11.84 LLTCTASVDD138 pKa = 3.99 VVEE141 pKa = 6.31 HH142 pKa = 6.71 SDD144 pKa = 3.26 QQ145 pKa = 3.66
Molecular weight: 16.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.356
IPC2_protein 9.516
IPC_protein 10.292
Toseland 10.511
ProMoST 10.233
Dawson 10.628
Bjellqvist 10.379
Wikipedia 10.847
Rodwell 10.789
Grimsley 10.687
Solomon 10.745
Lehninger 10.716
Nozaki 10.57
DTASelect 10.35
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.584
Patrickios 10.496
IPC_peptide 10.745
IPC2_peptide 9.75
IPC2.peptide.svr19 8.508
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21355
0
21355
8907007
24
5851
417.1
46.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.838 ± 0.014
1.882 ± 0.008
5.323 ± 0.012
6.562 ± 0.018
4.225 ± 0.01
6.883 ± 0.017
2.305 ± 0.007
5.075 ± 0.009
5.811 ± 0.014
9.816 ± 0.019
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.409 ± 0.006
4.22 ± 0.011
4.993 ± 0.016
3.506 ± 0.011
5.406 ± 0.012
8.885 ± 0.018
4.836 ± 0.01
6.845 ± 0.012
1.294 ± 0.006
2.755 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here