Aliiglaciecola lipolytica E3
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4327 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K6XUX9|K6XUX9_9ALTE Ubiquinone biosynthesis accessory factor UbiK OS=Aliiglaciecola lipolytica E3 OX=1127673 GN=ubiK PE=3 SV=1
MM1 pKa = 7.46 KK2 pKa = 10.05 IKK4 pKa = 10.83 YY5 pKa = 7.68 PALLALVCAPLAHH18 pKa = 6.97 ADD20 pKa = 3.41 VFINEE25 pKa = 3.75 FHH27 pKa = 7.01 YY28 pKa = 11.22 DD29 pKa = 3.4 NSSTDD34 pKa = 3.0 SGEE37 pKa = 4.34 AIEE40 pKa = 4.4 IAGTAGTDD48 pKa = 3.38 LSGWSLVLYY57 pKa = 10.06 NGSNGSVYY65 pKa = 10.11 DD66 pKa = 3.79 TTALSGSIPDD76 pKa = 3.65 QQNGMGTVFFSYY88 pKa = 10.25 PSNGIQNGPDD98 pKa = 3.57 AIALVDD104 pKa = 4.32 DD105 pKa = 4.69 SNSVIQFISYY115 pKa = 10.18 EE116 pKa = 4.34 GTFSASGGPANGMTSVNIGVSEE138 pKa = 4.2 SGSTNVGDD146 pKa = 4.15 SLQLSGTGSAYY157 pKa = 10.4 ADD159 pKa = 4.04 FAWQSAAANTFGAINNDD176 pKa = 3.09 QSFSANDD183 pKa = 3.7 GGGEE187 pKa = 4.26 DD188 pKa = 4.76 GGGDD192 pKa = 3.64 DD193 pKa = 4.31 SGSLEE198 pKa = 4.07 GVCFNCPDD206 pKa = 3.91 LDD208 pKa = 3.91 KK209 pKa = 11.51 VADD212 pKa = 3.79 ASEE215 pKa = 4.5 FDD217 pKa = 3.76 DD218 pKa = 3.99 ASYY221 pKa = 10.96 YY222 pKa = 10.82 AAAKK226 pKa = 9.62 TEE228 pKa = 3.74 IDD230 pKa = 3.81 MGSSSAIIKK239 pKa = 10.12 SALNGIITNDD249 pKa = 3.4 HH250 pKa = 6.35 RR251 pKa = 11.84 VLTYY255 pKa = 10.7 DD256 pKa = 3.78 QVWTALTEE264 pKa = 4.22 SDD266 pKa = 4.24 EE267 pKa = 5.34 DD268 pKa = 3.93 PANSDD273 pKa = 3.5 NVLLIYY279 pKa = 10.47 KK280 pKa = 9.69 GISLPKK286 pKa = 9.66 LSNGSGEE293 pKa = 4.26 QSTDD297 pKa = 3.08 PDD299 pKa = 2.98 NWNRR303 pKa = 11.84 EE304 pKa = 4.4 HH305 pKa = 6.31 VWPNSHH311 pKa = 6.47 GFPDD315 pKa = 4.18 RR316 pKa = 11.84 PFEE319 pKa = 4.47 AYY321 pKa = 9.4 TDD323 pKa = 3.46 IHH325 pKa = 6.55 HH326 pKa = 7.07 LRR328 pKa = 11.84 PSDD331 pKa = 3.09 ISVNGSRR338 pKa = 11.84 GNLDD342 pKa = 3.91 FDD344 pKa = 4.82 NSDD347 pKa = 3.61 APLDD351 pKa = 3.83 EE352 pKa = 5.23 APSNRR357 pKa = 11.84 IDD359 pKa = 3.37 SDD361 pKa = 3.68 SFEE364 pKa = 4.72 PRR366 pKa = 11.84 DD367 pKa = 3.61 EE368 pKa = 4.35 VKK370 pKa = 11.11 GDD372 pKa = 3.37 VARR375 pKa = 11.84 MMFYY379 pKa = 9.97 MDD381 pKa = 3.22 TRR383 pKa = 11.84 YY384 pKa = 10.51 EE385 pKa = 4.22 GLGDD389 pKa = 3.74 ATPDD393 pKa = 3.36 LVLSNGLTSAGQPQLGRR410 pKa = 11.84 LCRR413 pKa = 11.84 LIEE416 pKa = 3.92 WHH418 pKa = 6.58 NADD421 pKa = 4.07 PVDD424 pKa = 3.44 AAEE427 pKa = 4.2 QQRR430 pKa = 11.84 HH431 pKa = 4.3 DD432 pKa = 4.29 TIYY435 pKa = 10.52 EE436 pKa = 3.96 YY437 pKa = 10.71 QGNRR441 pKa = 11.84 NPFVDD446 pKa = 3.48 HH447 pKa = 6.97 PEE449 pKa = 3.95 WVSLLFTAEE458 pKa = 3.84 VCGDD462 pKa = 3.84 DD463 pKa = 5.92 DD464 pKa = 4.74 GGEE467 pKa = 4.29 DD468 pKa = 4.61 GGDD471 pKa = 3.56 DD472 pKa = 4.58 GEE474 pKa = 5.33 DD475 pKa = 3.49 SGNVGAVEE483 pKa = 4.1 ANALLITEE491 pKa = 4.76 IMQNPDD497 pKa = 3.88 TISDD501 pKa = 3.89 ANGEE505 pKa = 4.08 WFEE508 pKa = 4.14 VLNTSTQVINLNGWTIRR525 pKa = 11.84 DD526 pKa = 3.37 NDD528 pKa = 3.35 FDD530 pKa = 5.04 SFVVDD535 pKa = 3.04 QDD537 pKa = 3.57 VFIGVGEE544 pKa = 4.08 YY545 pKa = 10.81 AVLGKK550 pKa = 10.09 NADD553 pKa = 3.46 TSVNGGITLAYY564 pKa = 10.13 AYY566 pKa = 9.42 GGSMALANGSDD577 pKa = 3.87 EE578 pKa = 4.83 LVLVDD583 pKa = 4.04 PDD585 pKa = 4.0 GNIVDD590 pKa = 5.22 EE591 pKa = 4.26 ITYY594 pKa = 10.61 DD595 pKa = 3.67 GGPDD599 pKa = 3.57 FPDD602 pKa = 3.37 PTGASMYY609 pKa = 10.92 LVDD612 pKa = 6.26 LYY614 pKa = 11.62 GDD616 pKa = 3.75 NNVGSKK622 pKa = 8.47 WAADD626 pKa = 3.37 TTNFYY631 pKa = 11.28 DD632 pKa = 4.13 SNNSGTPGTGDD643 pKa = 2.85 ASFNLVITEE652 pKa = 4.07 IMQNPRR658 pKa = 11.84 AVGDD662 pKa = 3.73 SAGEE666 pKa = 3.85 YY667 pKa = 10.71 VEE669 pKa = 4.31 ILNTGVIAANLNGWVFRR686 pKa = 11.84 DD687 pKa = 3.55 NDD689 pKa = 4.32 SEE691 pKa = 4.56 SHH693 pKa = 6.58 TIQGDD698 pKa = 4.06 VYY700 pKa = 10.98 VPAGTYY706 pKa = 10.34 AVLTNNSDD714 pKa = 3.38 SSSNGGFEE722 pKa = 5.45 ALYY725 pKa = 9.94 QYY727 pKa = 11.2 SGVFIANGADD737 pKa = 3.12 EE738 pKa = 5.25 VVLQRR743 pKa = 11.84 PDD745 pKa = 3.06 GTIEE749 pKa = 5.7 DD750 pKa = 3.51 IVEE753 pKa = 4.06 YY754 pKa = 10.69 DD755 pKa = 3.76 GGPTFPDD762 pKa = 3.51 PNGASMTLISASLDD776 pKa = 3.51 NNVGEE781 pKa = 4.27 NWVEE785 pKa = 4.0 EE786 pKa = 4.32 TTATYY791 pKa = 11.28 GDD793 pKa = 4.36 GDD795 pKa = 4.12 FGTPGSGPDD804 pKa = 3.14 GKK806 pKa = 9.08 TFGGGDD812 pKa = 4.16 DD813 pKa = 4.41 GPGDD817 pKa = 4.08 VDD819 pKa = 4.44 PEE821 pKa = 4.2 IGVCTDD827 pKa = 3.57 PATLISTIQGNDD839 pKa = 3.17 FASLLDD845 pKa = 3.99 GQEE848 pKa = 4.61 HH849 pKa = 5.83 IVEE852 pKa = 4.25 AVVTSTLTDD861 pKa = 3.74 FGGFYY866 pKa = 10.52 LQEE869 pKa = 3.8 EE870 pKa = 4.61 DD871 pKa = 4.66 ADD873 pKa = 3.93 VDD875 pKa = 3.88 ADD877 pKa = 3.91 PATSEE882 pKa = 4.07 GLYY885 pKa = 10.57 VSYY888 pKa = 10.86 FDD890 pKa = 5.88 ANALPAQGTVVRR902 pKa = 11.84 VMGTVAEE909 pKa = 4.33 NFGRR913 pKa = 11.84 TQLNVSSDD921 pKa = 3.66 LVEE924 pKa = 4.97 CGTDD928 pKa = 3.25 TVSATTLVLPFSSALEE944 pKa = 4.18 AEE946 pKa = 4.61 SLEE949 pKa = 4.0 NMLVVNEE956 pKa = 4.48 SPLTVTNTYY965 pKa = 8.69 TLARR969 pKa = 11.84 FGEE972 pKa = 4.45 VGLSFGRR979 pKa = 11.84 LFNPTNVYY987 pKa = 10.88 APLSPEE993 pKa = 4.12 AVDD996 pKa = 5.36 LAAQNALNYY1005 pKa = 10.08 IIMDD1009 pKa = 4.73 DD1010 pKa = 3.98 GLDD1013 pKa = 3.53 VQNPEE1018 pKa = 3.96 TVIYY1022 pKa = 7.53 PTGNLSAANTLRR1034 pKa = 11.84 TGDD1037 pKa = 3.55 TVVNIKK1043 pKa = 10.41 GALDD1047 pKa = 3.44 YY1048 pKa = 11.17 SFSNFRR1054 pKa = 11.84 IHH1056 pKa = 7.52 PIEE1059 pKa = 4.43 TPTIIHH1065 pKa = 5.82 SNEE1068 pKa = 3.81 RR1069 pKa = 11.84 EE1070 pKa = 3.91 PAPSITRR1077 pKa = 11.84 GNLTVASLNVLNLFNGDD1094 pKa = 3.41 GQGGGFPTEE1103 pKa = 4.18 RR1104 pKa = 11.84 GADD1107 pKa = 3.33 SLFEE1111 pKa = 4.05 YY1112 pKa = 10.21 EE1113 pKa = 4.06 RR1114 pKa = 11.84 QIVKK1118 pKa = 8.95 TVEE1121 pKa = 4.62 AISTMDD1127 pKa = 3.62 ADD1129 pKa = 3.41 IVGLMEE1135 pKa = 4.75 IEE1137 pKa = 3.66 NDD1139 pKa = 3.61 GFGEE1143 pKa = 4.24 FSMVAEE1149 pKa = 4.28 LTNRR1153 pKa = 11.84 LNEE1156 pKa = 3.97 VMGEE1160 pKa = 4.04 GTYY1163 pKa = 11.21 DD1164 pKa = 3.45 FVSYY1168 pKa = 10.56 SSEE1171 pKa = 3.72 IGTDD1175 pKa = 4.1 AIAVALLYY1183 pKa = 10.68 KK1184 pKa = 9.82 PASVTLDD1191 pKa = 3.13 GDD1193 pKa = 3.71 VKK1195 pKa = 11.16 INFDD1199 pKa = 4.36 SIFNRR1204 pKa = 11.84 PPVAQSFTTLNGAGITVVVNHH1225 pKa = 6.64 FKK1227 pKa = 11.19 SKK1229 pKa = 10.25 GCGSASGDD1237 pKa = 4.02 DD1238 pKa = 3.74 TDD1240 pKa = 4.5 QGDD1243 pKa = 4.04 GQGCYY1248 pKa = 8.24 NAKK1251 pKa = 8.79 RR1252 pKa = 11.84 TQQSLSLASWLASEE1266 pKa = 5.05 EE1267 pKa = 4.16 SLSTKK1272 pKa = 10.43 EE1273 pKa = 3.74 NVLIIGDD1280 pKa = 4.23 LNAYY1284 pKa = 9.97 AKK1286 pKa = 9.71 EE1287 pKa = 4.06 DD1288 pKa = 4.53 PIAALEE1294 pKa = 4.22 GQGFVNLVEE1303 pKa = 4.32 TFQGAEE1309 pKa = 3.92 AYY1311 pKa = 10.2 SYY1313 pKa = 9.83 TFSGEE1318 pKa = 3.73 FGYY1321 pKa = 10.78 LDD1323 pKa = 3.55 HH1324 pKa = 7.28 ALASSSLAAQAVDD1337 pKa = 3.69 TIEE1340 pKa = 3.61 WHH1342 pKa = 6.61 INADD1346 pKa = 3.53 EE1347 pKa = 4.49 PFALDD1352 pKa = 3.4 YY1353 pKa = 11.21 NVEE1356 pKa = 4.45 FKK1358 pKa = 11.08 SDD1360 pKa = 3.19 AQVNDD1365 pKa = 5.39 FYY1367 pKa = 11.61 ASDD1370 pKa = 3.66 VFRR1373 pKa = 11.84 VSDD1376 pKa = 4.17 HH1377 pKa = 7.21 DD1378 pKa = 3.72 PVMMSFEE1385 pKa = 4.26 LASPAVEE1392 pKa = 4.12 GDD1394 pKa = 2.67 IDD1396 pKa = 4.46 GDD1398 pKa = 3.63 MDD1400 pKa = 3.94 VDD1402 pKa = 4.17 YY1403 pKa = 11.75 NDD1405 pKa = 3.49 MRR1407 pKa = 11.84 ALMNLIRR1414 pKa = 11.84 AGQATLEE1421 pKa = 4.06 EE1422 pKa = 4.65 HH1423 pKa = 7.33 DD1424 pKa = 4.49 FNNDD1428 pKa = 2.68 GLLNSGDD1435 pKa = 3.63 VSALRR1440 pKa = 11.84 SMCSRR1445 pKa = 11.84 RR1446 pKa = 11.84 ACSTRR1451 pKa = 3.02
Molecular weight: 154.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.694
IPC_protein 3.745
Toseland 3.516
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.567
Grimsley 3.427
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.101
Thurlkill 3.567
EMBOSS 3.681
Sillero 3.872
Patrickios 1.494
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.765
Protein with the highest isoelectric point:
>tr|K6WWA3|K6WWA3_9ALTE Sodium/glucose cotransporter OS=Aliiglaciecola lipolytica E3 OX=1127673 GN=sglT PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.14 RR12 pKa = 11.84 KK13 pKa = 9.13 RR14 pKa = 11.84 SHH16 pKa = 6.17 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 9.2 VLANRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.38 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4327
0
4327
1425066
37
3016
329.3
36.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.203 ± 0.034
0.984 ± 0.012
5.717 ± 0.031
6.121 ± 0.029
4.411 ± 0.026
6.535 ± 0.038
2.164 ± 0.016
6.64 ± 0.027
5.537 ± 0.038
10.2 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.442 ± 0.02
4.838 ± 0.026
3.828 ± 0.02
4.832 ± 0.034
4.223 ± 0.031
6.927 ± 0.031
5.277 ± 0.028
6.763 ± 0.03
1.255 ± 0.014
3.102 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here