Amino acid dipepetide frequency for Pseudomonas phage JBD25

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.816AlaAla: 17.816 ± 3.106
1.273AlaCys: 1.273 ± 0.405
6.761AlaAsp: 6.761 ± 0.661
8.192AlaGlu: 8.192 ± 0.796
3.818AlaPhe: 3.818 ± 0.621
9.624AlaGly: 9.624 ± 0.972
1.113AlaHis: 1.113 ± 0.359
4.852AlaIle: 4.852 ± 0.444
5.17AlaLys: 5.17 ± 0.79
13.203AlaLeu: 13.203 ± 1.102
3.738AlaMet: 3.738 ± 0.537
2.704AlaAsn: 2.704 ± 0.484
5.09AlaPro: 5.09 ± 0.841
6.283AlaGln: 6.283 ± 0.901
7.794AlaArg: 7.794 ± 0.824
6.363AlaSer: 6.363 ± 0.759
6.363AlaThr: 6.363 ± 0.946
6.601AlaVal: 6.601 ± 0.669
2.545AlaTrp: 2.545 ± 0.432
2.466AlaTyr: 2.466 ± 0.436
0.0AlaXaa: 0.0 ± 0.0
Cys
0.795CysAla: 0.795 ± 0.291
0.239CysCys: 0.239 ± 0.138
0.795CysAsp: 0.795 ± 0.252
0.557CysGlu: 0.557 ± 0.188
0.318CysPhe: 0.318 ± 0.151
0.875CysGly: 0.875 ± 0.295
0.318CysHis: 0.318 ± 0.156
0.08CysIle: 0.08 ± 0.075
0.398CysLys: 0.398 ± 0.17
0.398CysLeu: 0.398 ± 0.158
0.0CysMet: 0.0 ± 0.0
0.398CysAsn: 0.398 ± 0.187
0.557CysPro: 0.557 ± 0.191
0.318CysGln: 0.318 ± 0.141
0.795CysArg: 0.795 ± 0.311
0.795CysSer: 0.795 ± 0.256
0.636CysThr: 0.636 ± 0.217
0.557CysVal: 0.557 ± 0.213
0.08CysTrp: 0.08 ± 0.081
0.239CysTyr: 0.239 ± 0.131
0.0CysXaa: 0.0 ± 0.0
Asp
6.522AspAla: 6.522 ± 0.777
0.239AspCys: 0.239 ± 0.139
3.181AspAsp: 3.181 ± 0.472
4.772AspGlu: 4.772 ± 0.577
2.227AspPhe: 2.227 ± 0.42
6.283AspGly: 6.283 ± 0.7
1.273AspHis: 1.273 ± 0.344
2.466AspIle: 2.466 ± 0.432
1.432AspLys: 1.432 ± 0.298
5.647AspLeu: 5.647 ± 0.551
1.113AspMet: 1.113 ± 0.26
1.511AspAsn: 1.511 ± 0.306
2.863AspPro: 2.863 ± 0.455
3.659AspGln: 3.659 ± 0.699
3.261AspArg: 3.261 ± 0.527
2.545AspSer: 2.545 ± 0.349
2.784AspThr: 2.784 ± 0.363
4.693AspVal: 4.693 ± 0.519
0.795AspTrp: 0.795 ± 0.183
1.591AspTyr: 1.591 ± 0.396
0.0AspXaa: 0.0 ± 0.0
Glu
7.397GluAla: 7.397 ± 1.028
0.636GluCys: 0.636 ± 0.207
3.897GluAsp: 3.897 ± 0.685
4.215GluGlu: 4.215 ± 0.681
1.67GluPhe: 1.67 ± 0.348
2.307GluGly: 2.307 ± 0.425
1.511GluHis: 1.511 ± 0.419
3.022GluIle: 3.022 ± 0.397
3.5GluLys: 3.5 ± 0.566
6.999GluLeu: 6.999 ± 0.777
1.432GluMet: 1.432 ± 0.381
1.988GluAsn: 1.988 ± 0.431
1.829GluPro: 1.829 ± 0.427
4.852GluGln: 4.852 ± 0.717
5.488GluArg: 5.488 ± 0.753
3.34GluSer: 3.34 ± 0.627
2.068GluThr: 2.068 ± 0.345
3.5GluVal: 3.5 ± 0.582
1.273GluTrp: 1.273 ± 0.287
1.67GluTyr: 1.67 ± 0.293
0.0GluXaa: 0.0 ± 0.0
Phe
2.943PheAla: 2.943 ± 0.483
0.239PheCys: 0.239 ± 0.138
2.863PheAsp: 2.863 ± 0.463
1.75PheGlu: 1.75 ± 0.33
0.875PhePhe: 0.875 ± 0.279
3.102PheGly: 3.102 ± 0.425
0.477PheHis: 0.477 ± 0.177
1.193PheIle: 1.193 ± 0.235
0.795PheLys: 0.795 ± 0.251
2.545PheLeu: 2.545 ± 0.373
0.636PheMet: 0.636 ± 0.22
1.352PheAsn: 1.352 ± 0.37
0.795PhePro: 0.795 ± 0.25
1.273PheGln: 1.273 ± 0.361
2.386PheArg: 2.386 ± 0.469
1.67PheSer: 1.67 ± 0.384
1.511PheThr: 1.511 ± 0.38
1.352PheVal: 1.352 ± 0.36
0.398PheTrp: 0.398 ± 0.181
0.636PheTyr: 0.636 ± 0.247
0.0PheXaa: 0.0 ± 0.0
Gly
7.635GlyAla: 7.635 ± 1.302
0.954GlyCys: 0.954 ± 0.311
4.374GlyAsp: 4.374 ± 0.623
4.136GlyGlu: 4.136 ± 0.462
2.386GlyPhe: 2.386 ± 0.33
4.852GlyGly: 4.852 ± 0.661
1.432GlyHis: 1.432 ± 0.386
3.34GlyIle: 3.34 ± 0.363
4.374GlyLys: 4.374 ± 0.589
7.556GlyLeu: 7.556 ± 0.875
2.386GlyMet: 2.386 ± 0.482
3.42GlyAsn: 3.42 ± 0.515
1.909GlyPro: 1.909 ± 0.426
4.613GlyGln: 4.613 ± 0.684
4.534GlyArg: 4.534 ± 0.635
3.977GlySer: 3.977 ± 0.854
3.977GlyThr: 3.977 ± 0.545
5.17GlyVal: 5.17 ± 0.617
2.147GlyTrp: 2.147 ± 0.378
2.625GlyTyr: 2.625 ± 0.451
0.0GlyXaa: 0.0 ± 0.0
His
1.909HisAla: 1.909 ± 0.476
0.159HisCys: 0.159 ± 0.117
0.875HisAsp: 0.875 ± 0.229
0.954HisGlu: 0.954 ± 0.301
0.477HisPhe: 0.477 ± 0.218
1.75HisGly: 1.75 ± 0.448
0.239HisHis: 0.239 ± 0.141
0.716HisIle: 0.716 ± 0.197
0.636HisLys: 0.636 ± 0.202
2.227HisLeu: 2.227 ± 0.481
0.159HisMet: 0.159 ± 0.111
0.636HisAsn: 0.636 ± 0.242
1.193HisPro: 1.193 ± 0.391
1.273HisGln: 1.273 ± 0.337
1.273HisArg: 1.273 ± 0.319
1.352HisSer: 1.352 ± 0.349
0.636HisThr: 0.636 ± 0.225
1.193HisVal: 1.193 ± 0.258
0.159HisTrp: 0.159 ± 0.115
0.239HisTyr: 0.239 ± 0.128
0.0HisXaa: 0.0 ± 0.0
Ile
5.329IleAla: 5.329 ± 0.737
0.477IleCys: 0.477 ± 0.206
3.102IleAsp: 3.102 ± 0.474
4.374IleGlu: 4.374 ± 0.537
0.557IlePhe: 0.557 ± 0.229
2.466IleGly: 2.466 ± 0.471
0.954IleHis: 0.954 ± 0.221
1.193IleIle: 1.193 ± 0.309
1.432IleLys: 1.432 ± 0.282
3.34IleLeu: 3.34 ± 0.497
0.318IleMet: 0.318 ± 0.171
1.67IleAsn: 1.67 ± 0.418
2.307IlePro: 2.307 ± 0.442
1.75IleGln: 1.75 ± 0.317
3.181IleArg: 3.181 ± 0.422
1.909IleSer: 1.909 ± 0.49
3.5IleThr: 3.5 ± 0.641
1.909IleVal: 1.909 ± 0.41
0.636IleTrp: 0.636 ± 0.247
1.034IleTyr: 1.034 ± 0.288
0.0IleXaa: 0.0 ± 0.0
Lys
5.647LysAla: 5.647 ± 0.87
0.239LysCys: 0.239 ± 0.166
2.068LysAsp: 2.068 ± 0.453
2.466LysGlu: 2.466 ± 0.516
0.795LysPhe: 0.795 ± 0.226
2.863LysGly: 2.863 ± 0.565
0.716LysHis: 0.716 ± 0.201
1.432LysIle: 1.432 ± 0.309
1.67LysLys: 1.67 ± 0.351
3.897LysLeu: 3.897 ± 0.651
0.318LysMet: 0.318 ± 0.164
1.193LysAsn: 1.193 ± 0.299
2.466LysPro: 2.466 ± 0.608
1.75LysGln: 1.75 ± 0.372
4.772LysArg: 4.772 ± 0.663
2.227LysSer: 2.227 ± 0.431
2.307LysThr: 2.307 ± 0.446
2.227LysVal: 2.227 ± 0.409
0.318LysTrp: 0.318 ± 0.151
0.795LysTyr: 0.795 ± 0.184
0.0LysXaa: 0.0 ± 0.0
Leu
12.646LeuAla: 12.646 ± 1.011
0.795LeuCys: 0.795 ± 0.299
6.999LeuAsp: 6.999 ± 0.618
5.408LeuGlu: 5.408 ± 0.644
2.307LeuPhe: 2.307 ± 0.436
7.397LeuGly: 7.397 ± 0.872
2.147LeuHis: 2.147 ± 0.407
3.579LeuIle: 3.579 ± 0.47
3.897LeuLys: 3.897 ± 0.557
8.51LeuLeu: 8.51 ± 0.774
1.909LeuMet: 1.909 ± 0.335
3.022LeuAsn: 3.022 ± 0.572
5.249LeuPro: 5.249 ± 0.653
5.567LeuGln: 5.567 ± 0.757
7.874LeuArg: 7.874 ± 0.871
5.727LeuSer: 5.727 ± 0.703
6.124LeuThr: 6.124 ± 0.885
7.874LeuVal: 7.874 ± 0.83
0.795LeuTrp: 0.795 ± 0.244
2.386LeuTyr: 2.386 ± 0.468
0.0LeuXaa: 0.0 ± 0.0
Met
3.34MetAla: 3.34 ± 0.48
0.0MetCys: 0.0 ± 0.0
1.113MetAsp: 1.113 ± 0.28
1.67MetGlu: 1.67 ± 0.368
0.477MetPhe: 0.477 ± 0.174
1.988MetGly: 1.988 ± 0.274
0.477MetHis: 0.477 ± 0.204
1.273MetIle: 1.273 ± 0.418
1.034MetLys: 1.034 ± 0.261
1.511MetLeu: 1.511 ± 0.359
0.08MetMet: 0.08 ± 0.085
0.557MetAsn: 0.557 ± 0.216
1.193MetPro: 1.193 ± 0.379
1.113MetGln: 1.113 ± 0.319
1.432MetArg: 1.432 ± 0.313
1.829MetSer: 1.829 ± 0.378
1.273MetThr: 1.273 ± 0.32
1.113MetVal: 1.113 ± 0.306
0.239MetTrp: 0.239 ± 0.133
0.239MetTyr: 0.239 ± 0.172
0.0MetXaa: 0.0 ± 0.0
Asn
3.579AsnAla: 3.579 ± 0.453
0.08AsnCys: 0.08 ± 0.079
1.034AsnAsp: 1.034 ± 0.25
1.67AsnGlu: 1.67 ± 0.378
0.636AsnPhe: 0.636 ± 0.243
3.102AsnGly: 3.102 ± 0.606
0.636AsnHis: 0.636 ± 0.23
1.034AsnIle: 1.034 ± 0.345
1.034AsnLys: 1.034 ± 0.265
3.659AsnLeu: 3.659 ± 0.533
0.477AsnMet: 0.477 ± 0.202
0.795AsnAsn: 0.795 ± 0.322
2.068AsnPro: 2.068 ± 0.451
1.75AsnGln: 1.75 ± 0.41
2.147AsnArg: 2.147 ± 0.378
1.511AsnSer: 1.511 ± 0.312
1.75AsnThr: 1.75 ± 0.505
1.591AsnVal: 1.591 ± 0.263
0.557AsnTrp: 0.557 ± 0.21
1.352AsnTyr: 1.352 ± 0.277
0.0AsnXaa: 0.0 ± 0.0
Pro
7.079ProAla: 7.079 ± 0.728
0.318ProCys: 0.318 ± 0.154
3.34ProAsp: 3.34 ± 0.68
2.545ProGlu: 2.545 ± 0.441
1.591ProPhe: 1.591 ± 0.355
3.261ProGly: 3.261 ± 0.548
0.398ProHis: 0.398 ± 0.166
1.432ProIle: 1.432 ± 0.347
1.67ProLys: 1.67 ± 0.358
4.215ProLeu: 4.215 ± 0.574
1.432ProMet: 1.432 ± 0.351
1.273ProAsn: 1.273 ± 0.282
2.307ProPro: 2.307 ± 0.575
1.432ProGln: 1.432 ± 0.283
3.897ProArg: 3.897 ± 0.514
2.943ProSer: 2.943 ± 0.406
3.102ProThr: 3.102 ± 0.444
3.102ProVal: 3.102 ± 0.593
0.954ProTrp: 0.954 ± 0.29
1.829ProTyr: 1.829 ± 0.431
0.0ProXaa: 0.0 ± 0.0
Gln
7.317GlnAla: 7.317 ± 0.97
0.398GlnCys: 0.398 ± 0.177
2.307GlnAsp: 2.307 ± 0.409
3.579GlnGlu: 3.579 ± 0.729
2.068GlnPhe: 2.068 ± 0.436
2.704GlnGly: 2.704 ± 0.418
0.795GlnHis: 0.795 ± 0.259
1.75GlnIle: 1.75 ± 0.373
1.75GlnLys: 1.75 ± 0.57
6.84GlnLeu: 6.84 ± 0.733
0.954GlnMet: 0.954 ± 0.278
1.113GlnAsn: 1.113 ± 0.337
3.022GlnPro: 3.022 ± 0.452
3.261GlnGln: 3.261 ± 0.662
3.5GlnArg: 3.5 ± 0.449
2.625GlnSer: 2.625 ± 0.456
1.988GlnThr: 1.988 ± 0.321
4.056GlnVal: 4.056 ± 0.439
0.716GlnTrp: 0.716 ± 0.228
1.193GlnTyr: 1.193 ± 0.378
0.0GlnXaa: 0.0 ± 0.0
Arg
6.92ArgAla: 6.92 ± 0.78
0.795ArgCys: 0.795 ± 0.268
4.613ArgAsp: 4.613 ± 0.525
4.136ArgGlu: 4.136 ± 0.758
1.75ArgPhe: 1.75 ± 0.356
5.17ArgGly: 5.17 ± 0.605
2.068ArgHis: 2.068 ± 0.434
4.374ArgIle: 4.374 ± 0.482
3.181ArgLys: 3.181 ± 0.784
8.431ArgLeu: 8.431 ± 0.721
1.432ArgMet: 1.432 ± 0.342
1.909ArgAsn: 1.909 ± 0.402
2.625ArgPro: 2.625 ± 0.575
3.897ArgGln: 3.897 ± 0.715
6.204ArgArg: 6.204 ± 0.98
4.931ArgSer: 4.931 ± 0.648
2.068ArgThr: 2.068 ± 0.374
4.454ArgVal: 4.454 ± 0.663
1.352ArgTrp: 1.352 ± 0.337
2.466ArgTyr: 2.466 ± 0.437
0.0ArgXaa: 0.0 ± 0.0
Ser
7.476SerAla: 7.476 ± 0.833
0.318SerCys: 0.318 ± 0.14
3.102SerAsp: 3.102 ± 0.476
3.34SerGlu: 3.34 ± 0.423
2.227SerPhe: 2.227 ± 0.427
4.852SerGly: 4.852 ± 0.631
0.795SerHis: 0.795 ± 0.223
2.307SerIle: 2.307 ± 0.356
2.386SerLys: 2.386 ± 0.576
5.647SerLeu: 5.647 ± 0.606
1.432SerMet: 1.432 ± 0.351
1.988SerAsn: 1.988 ± 0.406
2.863SerPro: 2.863 ± 0.537
2.068SerGln: 2.068 ± 0.418
3.34SerArg: 3.34 ± 0.544
3.659SerSer: 3.659 ± 0.561
3.34SerThr: 3.34 ± 0.562
3.102SerVal: 3.102 ± 0.535
1.113SerTrp: 1.113 ± 0.371
1.591SerTyr: 1.591 ± 0.363
0.0SerXaa: 0.0 ± 0.0
Thr
6.84ThrAla: 6.84 ± 0.867
0.716ThrCys: 0.716 ± 0.293
2.625ThrAsp: 2.625 ± 0.509
2.386ThrGlu: 2.386 ± 0.482
1.67ThrPhe: 1.67 ± 0.319
5.965ThrGly: 5.965 ± 1.118
0.875ThrHis: 0.875 ± 0.27
1.67ThrIle: 1.67 ± 0.381
1.75ThrLys: 1.75 ± 0.345
5.09ThrLeu: 5.09 ± 0.815
0.954ThrMet: 0.954 ± 0.294
1.988ThrAsn: 1.988 ± 0.342
3.5ThrPro: 3.5 ± 0.429
1.829ThrGln: 1.829 ± 0.405
2.863ThrArg: 2.863 ± 0.458
3.181ThrSer: 3.181 ± 0.416
3.181ThrThr: 3.181 ± 0.739
4.136ThrVal: 4.136 ± 0.76
1.034ThrTrp: 1.034 ± 0.337
1.352ThrTyr: 1.352 ± 0.366
0.0ThrXaa: 0.0 ± 0.0
Val
6.601ValAla: 6.601 ± 0.768
0.636ValCys: 0.636 ± 0.202
4.295ValAsp: 4.295 ± 0.536
4.295ValGlu: 4.295 ± 0.719
1.511ValPhe: 1.511 ± 0.331
4.215ValGly: 4.215 ± 0.572
1.193ValHis: 1.193 ± 0.252
3.5ValIle: 3.5 ± 0.555
2.466ValLys: 2.466 ± 0.473
5.806ValLeu: 5.806 ± 0.588
1.352ValMet: 1.352 ± 0.337
1.511ValAsn: 1.511 ± 0.291
3.977ValPro: 3.977 ± 0.409
2.943ValGln: 2.943 ± 0.526
4.136ValArg: 4.136 ± 0.643
3.42ValSer: 3.42 ± 0.561
4.693ValThr: 4.693 ± 0.673
4.772ValVal: 4.772 ± 0.969
1.273ValTrp: 1.273 ± 0.332
1.591ValTyr: 1.591 ± 0.478
0.0ValXaa: 0.0 ± 0.0
Trp
1.352TrpAla: 1.352 ± 0.28
0.318TrpCys: 0.318 ± 0.143
0.398TrpAsp: 0.398 ± 0.168
0.318TrpGlu: 0.318 ± 0.143
0.716TrpPhe: 0.716 ± 0.199
1.034TrpGly: 1.034 ± 0.357
0.08TrpHis: 0.08 ± 0.077
1.034TrpIle: 1.034 ± 0.345
0.477TrpLys: 0.477 ± 0.186
2.307TrpLeu: 2.307 ± 0.442
0.795TrpMet: 0.795 ± 0.28
0.477TrpAsn: 0.477 ± 0.17
0.636TrpPro: 0.636 ± 0.225
1.273TrpGln: 1.273 ± 0.339
1.511TrpArg: 1.511 ± 0.224
1.273TrpSer: 1.273 ± 0.288
1.034TrpThr: 1.034 ± 0.282
1.511TrpVal: 1.511 ± 0.369
0.557TrpTrp: 0.557 ± 0.263
0.239TrpTyr: 0.239 ± 0.117
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.625TyrAla: 2.625 ± 0.52
0.318TyrCys: 0.318 ± 0.163
1.113TyrAsp: 1.113 ± 0.331
1.829TyrGlu: 1.829 ± 0.368
0.716TyrPhe: 0.716 ± 0.222
1.988TyrGly: 1.988 ± 0.442
0.557TyrHis: 0.557 ± 0.289
1.193TyrIle: 1.193 ± 0.317
1.113TyrLys: 1.113 ± 0.28
2.386TyrLeu: 2.386 ± 0.418
0.954TyrMet: 0.954 ± 0.362
0.954TyrAsn: 0.954 ± 0.235
1.75TyrPro: 1.75 ± 0.482
1.034TyrGln: 1.034 ± 0.314
2.386TyrArg: 2.386 ± 0.426
1.67TyrSer: 1.67 ± 0.423
1.273TyrThr: 1.273 ± 0.378
1.273TyrVal: 1.273 ± 0.293
0.398TyrTrp: 0.398 ± 0.218
0.318TyrTyr: 0.318 ± 0.164
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (12574 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski