Limnothrix sp. P13C2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Pseudanabaenaceae; Limnothrix; unclassified Limnothrix

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3670 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C0V632|A0A1C0V632_9CYAN Transposase OS=Limnothrix sp. P13C2 OX=1880902 GN=BCR12_13195 PE=4 SV=1
MM1 pKa = 6.99TQFGNNLPNFLIGSGVDD18 pKa = 3.34TLIGLGDD25 pKa = 4.14ADD27 pKa = 4.19SLVSSTAGRR36 pKa = 11.84NAIFGNAGADD46 pKa = 3.56SLISQGGADD55 pKa = 4.31TIYY58 pKa = 10.39GGQGADD64 pKa = 3.34IIRR67 pKa = 11.84SRR69 pKa = 11.84GGSLLYY75 pKa = 10.56GDD77 pKa = 5.23RR78 pKa = 11.84GNDD81 pKa = 3.74TIVGEE86 pKa = 4.27ASSTGIIGGSTAGGGDD102 pKa = 3.29TVYY105 pKa = 10.86GGEE108 pKa = 4.42GDD110 pKa = 4.29DD111 pKa = 3.74SLVANSVGPSSLFGNQGNDD130 pKa = 3.05VLVAGNRR137 pKa = 11.84ADD139 pKa = 3.55TLYY142 pKa = 10.97GGQGNDD148 pKa = 3.28TLTSTVGGLLYY159 pKa = 10.7GDD161 pKa = 4.95RR162 pKa = 11.84GNDD165 pKa = 3.69SIVLSGSGVAGATTVYY181 pKa = 10.86GGDD184 pKa = 3.52GDD186 pKa = 4.75DD187 pKa = 5.22AITGGGRR194 pKa = 11.84TLLFGNQGNDD204 pKa = 3.29TITAGAADD212 pKa = 4.04SVYY215 pKa = 10.3GGSGADD221 pKa = 3.29VLTASGSGAFISGDD235 pKa = 3.45LGNDD239 pKa = 2.86ILRR242 pKa = 11.84NTGANNTLAGGEE254 pKa = 4.13GDD256 pKa = 4.07DD257 pKa = 4.79TITFDD262 pKa = 3.96GGAAFSTASGGGGKK276 pKa = 9.55NFLQILPTITGSGNVLIAGSLGDD299 pKa = 3.79TLVGAGANTIQGGAGDD315 pKa = 5.0DD316 pKa = 3.86SLVSQGSNVTLVGGAGRR333 pKa = 11.84DD334 pKa = 3.71TFNVLAGGAISGFNPGEE351 pKa = 4.14GDD353 pKa = 3.74TILSGTAPFTIIAGSTGLYY372 pKa = 8.89LTGTPNRR379 pKa = 11.84DD380 pKa = 3.37TLTGGAGNDD389 pKa = 3.57TLEE392 pKa = 4.43GLGNADD398 pKa = 3.87ILTGGAGADD407 pKa = 3.2VFLFKK412 pKa = 10.68GVAISDD418 pKa = 3.89VASVAFSNNVLQVGGTLGAGTQAAVVQDD446 pKa = 3.42VVYY449 pKa = 8.24FTSLPNPAHH458 pKa = 5.71GQSTGGNAAGTTATYY473 pKa = 10.91SFAGAGTVSSLGGTSGTFIAPPYY496 pKa = 9.58GAALSVTYY504 pKa = 8.43GTTGGVGTVVTPSFGSYY521 pKa = 8.45QATGTTAVVPVSGFGYY537 pKa = 10.46ALSGFDD543 pKa = 4.14TITDD547 pKa = 4.44FEE549 pKa = 4.83TGVDD553 pKa = 3.76QIRR556 pKa = 11.84LEE558 pKa = 4.09GRR560 pKa = 11.84LFSTGASFPNANAPGAFLSVTSITGTNVSNLVNKK594 pKa = 9.91TEE596 pKa = 4.04LLIYY600 pKa = 10.27AQDD603 pKa = 3.28SGILYY608 pKa = 9.93GRR610 pKa = 11.84AVAGGTNTNTAGTGLGSQYY629 pKa = 10.7VYY631 pKa = 11.25SPTPVPAFGTLASLNGPGDD650 pKa = 3.4QKK652 pKa = 11.53VIISSPGTVSVVGTNSNHH670 pKa = 5.75VGTVQYY676 pKa = 10.67FLLPQTPISGVAFSNSGTNTVSSVLPIPFAQVLRR710 pKa = 11.84GGAPVTGLTYY720 pKa = 10.86GRR722 pKa = 11.84DD723 pKa = 3.03IVIFF727 pKa = 4.02

Molecular weight: 70.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C0V266|A0A1C0V266_9CYAN XisI protein OS=Limnothrix sp. P13C2 OX=1880902 GN=BCR12_01825 PE=4 SV=1
MM1 pKa = 7.34AKK3 pKa = 9.55SWVSRR8 pKa = 11.84SIEE11 pKa = 4.05ALSDD15 pKa = 3.89LVWQRR20 pKa = 11.84SCEE23 pKa = 4.21LCDD26 pKa = 4.76RR27 pKa = 11.84PAKK30 pKa = 10.37HH31 pKa = 6.34QGLCLACDD39 pKa = 3.53RR40 pKa = 11.84RR41 pKa = 11.84LLASQLPLEE50 pKa = 4.44QRR52 pKa = 11.84WGRR55 pKa = 11.84LPSALVSSAPLFLAWGLYY73 pKa = 10.21DD74 pKa = 4.12GSLKK78 pKa = 10.49RR79 pKa = 11.84AIAALKK85 pKa = 10.71YY86 pKa = 9.78EE87 pKa = 4.26GHH89 pKa = 7.08PKK91 pKa = 9.93LAATLGQGLGRR102 pKa = 11.84LWLEE106 pKa = 4.32PGTEE110 pKa = 3.79RR111 pKa = 11.84AMATPWQQLDD121 pKa = 3.62RR122 pKa = 11.84AAPWVIPIPLHH133 pKa = 5.12EE134 pKa = 5.28ARR136 pKa = 11.84LQKK139 pKa = 10.55RR140 pKa = 11.84GFNQAEE146 pKa = 4.04LLAKK150 pKa = 9.63HH151 pKa = 5.89FCDD154 pKa = 3.33VTGYY158 pKa = 11.02RR159 pKa = 11.84LIPNGLQRR167 pKa = 11.84IRR169 pKa = 11.84DD170 pKa = 3.82TQAQFGLGQGDD181 pKa = 3.29RR182 pKa = 11.84AANVQGAFALGPGLGQQRR200 pKa = 11.84PRR202 pKa = 11.84SVILLDD208 pKa = 5.2DD209 pKa = 4.8IYY211 pKa = 11.13TSGATVQAASNSLRR225 pKa = 11.84AGGWTVAAVVVVARR239 pKa = 11.84SAQRR243 pKa = 11.84PAPGTHH249 pKa = 6.92PPTRR253 pKa = 11.84SAPTRR258 pKa = 11.84PKK260 pKa = 10.68RR261 pKa = 11.84RR262 pKa = 11.84DD263 pKa = 3.1SS264 pKa = 3.46

Molecular weight: 28.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3670

0

3670

1208963

29

9652

329.4

36.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.418 ± 0.058

0.97 ± 0.012

5.526 ± 0.037

5.643 ± 0.041

3.479 ± 0.026

7.461 ± 0.047

1.794 ± 0.018

5.348 ± 0.032

2.955 ± 0.037

11.479 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.821 ± 0.018

3.171 ± 0.03

5.733 ± 0.045

5.363 ± 0.034

6.693 ± 0.036

5.983 ± 0.033

5.306 ± 0.026

6.616 ± 0.031

1.752 ± 0.024

2.487 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski