[Candida] intermedia

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Metschnikowiaceae; Clavispora; Clavispora/Candida clade

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5921 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L0CSD2|A0A1L0CSD2_9ASCO DNA polymerase OS=[Candida] intermedia OX=45354 GN=SAMEA4029010_CIC11G00000002857 PE=3 SV=1
MM1 pKa = 7.59ILHH4 pKa = 6.57EE5 pKa = 4.55FVSLALLATVVQAATTTVSTTSTFPFTSYY34 pKa = 10.52SQSSTDD40 pKa = 2.79QWLIEE45 pKa = 4.25TDD47 pKa = 3.51GSLTFSSILNLKK59 pKa = 7.91TGKK62 pKa = 9.8NFEE65 pKa = 4.41NYY67 pKa = 10.62GDD69 pKa = 4.04VIMNANPGATLEE81 pKa = 4.19MDD83 pKa = 3.46SLTNYY88 pKa = 7.24EE89 pKa = 4.16TGSVKK94 pKa = 10.11IAASGGSLTFQSSNTYY110 pKa = 8.83TNQGLWVMSTPGDD123 pKa = 3.9LEE125 pKa = 4.11VDD127 pKa = 3.92LTDD130 pKa = 4.85SLYY133 pKa = 10.68TLLNSGSLTYY143 pKa = 10.64EE144 pKa = 3.85SGGDD148 pKa = 3.42MAISVSKK155 pKa = 10.68VFINSEE161 pKa = 4.32EE162 pKa = 4.16VSFTASGKK170 pKa = 8.58GTFSFSSDD178 pKa = 3.36FTNSGTMIVTSSSVSDD194 pKa = 3.37TFKK197 pKa = 10.94ISDD200 pKa = 3.53TFVNDD205 pKa = 2.78NYY207 pKa = 10.67FVYY210 pKa = 10.54NYY212 pKa = 10.18GLNSGTAFSLSSLLSTYY229 pKa = 9.6TNNGIMNIYY238 pKa = 10.23GSTGSTNMNQDD249 pKa = 3.1GPIVNDD255 pKa = 3.77GSICLRR261 pKa = 11.84HH262 pKa = 4.4TTYY265 pKa = 11.24NGGAKK270 pKa = 10.43LSGTGCWVLADD281 pKa = 3.69SSVFTQHH288 pKa = 5.84VARR291 pKa = 11.84LANTQTIVFNDD302 pKa = 3.85TSASIRR308 pKa = 11.84VSSVGLGSHH317 pKa = 4.27YY318 pKa = 9.85TIYY321 pKa = 10.71GVQNGMTFLTSSLLIGSVVYY341 pKa = 10.86SPFTGILTLTLGLLSTTYY359 pKa = 10.61DD360 pKa = 2.67IGLGYY365 pKa = 10.51DD366 pKa = 3.29PTGFTVSSNNVRR378 pKa = 11.84YY379 pKa = 10.2VGNDD383 pKa = 3.12PVKK386 pKa = 10.43RR387 pKa = 11.84DD388 pKa = 3.36IPGEE392 pKa = 4.28CICEE396 pKa = 4.03PQEE399 pKa = 3.86QSSSVFSSSASSSSSISEE417 pKa = 4.21TSSEE421 pKa = 4.14ATSSQSSEE429 pKa = 3.89ATSSQSSEE437 pKa = 3.91VSSSQSSEE445 pKa = 3.75ASSSQSSEE453 pKa = 3.72ASSSQSSEE461 pKa = 3.72ASSSQSSEE469 pKa = 3.72ASSSQSSEE477 pKa = 3.78ATSSQSSEE485 pKa = 3.82ASSSQSSEE493 pKa = 3.72ASSSQSSEE501 pKa = 3.72ASSSQSSEE509 pKa = 3.72ASSSQSSEE517 pKa = 3.72ASSSQSSEE525 pKa = 3.72ASSSQSSEE533 pKa = 3.8AASSQSSEE541 pKa = 4.03ALSSQSSEE549 pKa = 4.07ASPQSSEE556 pKa = 4.07ASSSHH561 pKa = 6.62PASASNSQTEE571 pKa = 4.05QSTAPSVQTSQSVEE585 pKa = 3.61EE586 pKa = 4.29SQALNSNTEE595 pKa = 4.07YY596 pKa = 7.78TTTWTTTNSDD606 pKa = 3.26GSVEE610 pKa = 4.08TDD612 pKa = 2.95SGVVIVTTDD621 pKa = 3.01SAGVLTTSTSKK632 pKa = 10.87LSPAQYY638 pKa = 6.53TTTWTTTNSDD648 pKa = 3.26GSVEE652 pKa = 4.08TDD654 pKa = 2.96SGVVFVTTDD663 pKa = 2.95SAGVLTTSTSKK674 pKa = 10.87LSPAQYY680 pKa = 6.53TTTWTTTNSDD690 pKa = 3.26GSVEE694 pKa = 4.08TDD696 pKa = 2.95SGVVIVTTDD705 pKa = 3.01SAGVLTTSTSKK716 pKa = 10.87LSPAQYY722 pKa = 6.53TTTWTTTNSDD732 pKa = 3.26GSVEE736 pKa = 4.08TDD738 pKa = 2.95SGVVIVTTDD747 pKa = 3.01SAGVLTTSTSKK758 pKa = 10.66LAPAQYY764 pKa = 6.91TTTWTTTNSDD774 pKa = 3.26GSVEE778 pKa = 4.08TDD780 pKa = 2.96SGVVFVTTDD789 pKa = 2.95SAGVLTTSTSKK800 pKa = 10.87LSPAQYY806 pKa = 6.53TTTWTTTNSDD816 pKa = 3.26GSVEE820 pKa = 4.08TDD822 pKa = 2.95SGVVIVTTDD831 pKa = 3.01SAGVLTTSTSKK842 pKa = 10.9LSPAKK847 pKa = 9.75YY848 pKa = 5.3TTTWTTTNSDD858 pKa = 3.26GSVEE862 pKa = 4.08TDD864 pKa = 2.96SGVVFVTTDD873 pKa = 2.95SAGVLTTSTSKK884 pKa = 10.87LSPAQYY890 pKa = 6.53TTTWTTTNSDD900 pKa = 3.26GSVEE904 pKa = 4.08TDD906 pKa = 2.95SGVVIVTTDD915 pKa = 3.01SAGVLTTSTSKK926 pKa = 10.66LAPAQYY932 pKa = 6.91TTTWTTTNSDD942 pKa = 3.26GSVEE946 pKa = 4.08TDD948 pKa = 2.95SGVVIVTTDD957 pKa = 3.01SAGVLTTSTSKK968 pKa = 10.66LAPAQYY974 pKa = 6.91TTTWTTTNSDD984 pKa = 3.26GSVEE988 pKa = 4.08TDD990 pKa = 2.96SGVVFVTTDD999 pKa = 2.95SAGVLTTSTSKK1010 pKa = 10.66LAPAQYY1016 pKa = 6.91TTTWTTTNSDD1026 pKa = 3.26GSVEE1030 pKa = 4.08TDD1032 pKa = 2.95SGVVIVTTDD1041 pKa = 3.01SAGVLTTSTSKK1052 pKa = 10.66LAPAQYY1058 pKa = 6.91TTTWTTTNSDD1068 pKa = 3.26GSVEE1072 pKa = 4.08TDD1074 pKa = 2.96SGVVFVTTDD1083 pKa = 2.95SAGVLTTSTSKK1094 pKa = 10.66LAPAQYY1100 pKa = 6.91TTTWTTTNSDD1110 pKa = 3.26GSVEE1114 pKa = 4.08TDD1116 pKa = 2.95SGVVIVTTDD1125 pKa = 3.01SAGVLTTSTSKK1136 pKa = 10.87LSPAQYY1142 pKa = 6.53TTTWTTTNSDD1152 pKa = 3.26GSVEE1156 pKa = 4.08TDD1158 pKa = 2.96SGVVFVTTDD1167 pKa = 2.95SAGVLTTSTSKK1178 pKa = 10.66LAPAQYY1184 pKa = 6.91TTTWTTTNSDD1194 pKa = 3.26GSVEE1198 pKa = 4.08TDD1200 pKa = 2.96SGVVFVTTDD1209 pKa = 2.95SAGVLTTSTSKK1220 pKa = 10.9LSPAKK1225 pKa = 9.75YY1226 pKa = 5.3TTTWTTTNSDD1236 pKa = 3.26GSVEE1240 pKa = 4.08TDD1242 pKa = 2.96SGVVFVTTDD1251 pKa = 2.95SAGVLTTSTSKK1262 pKa = 10.87LSPAQYY1268 pKa = 6.53TTTWTTTNSDD1278 pKa = 3.26GSVEE1282 pKa = 4.08TDD1284 pKa = 2.95SGVVIVTTDD1293 pKa = 3.01SAGVLTTSTSKK1304 pKa = 10.66LAPAQYY1310 pKa = 6.91TTTWTTTNSDD1320 pKa = 3.26GSVEE1324 pKa = 4.08TDD1326 pKa = 2.95SGVVIVTTDD1335 pKa = 2.98SAGVFTTSTSKK1346 pKa = 10.66LAPAQYY1352 pKa = 6.91TTTWTTTNSDD1362 pKa = 3.26GSVEE1366 pKa = 4.08TDD1368 pKa = 2.96SGVVFVTTDD1377 pKa = 2.95SAGVLTTSTSKK1388 pKa = 10.66LAPAQYY1394 pKa = 6.91TTTWTTTNSDD1404 pKa = 3.26GSVEE1408 pKa = 4.08TDD1410 pKa = 2.95SGVVIVTTDD1419 pKa = 3.01SAGVLTTSTSKK1430 pKa = 10.87LSPAQYY1436 pKa = 6.53TTTWTTTNSDD1446 pKa = 3.26GSVEE1450 pKa = 4.08TDD1452 pKa = 2.95SGVVIVTTDD1461 pKa = 3.01SAGVLTTSTSKK1472 pKa = 10.66LAPAQYY1478 pKa = 6.91TTTWTTTNSDD1488 pKa = 3.26GSVEE1492 pKa = 4.08TDD1494 pKa = 2.95SGVVIVTTDD1503 pKa = 3.01SAGVLTTSTSKK1514 pKa = 10.66LAPAQYY1520 pKa = 6.91TTTWTTTNSDD1530 pKa = 3.26GSVEE1534 pKa = 4.08TDD1536 pKa = 2.96SGVVFVTTDD1545 pKa = 2.95SAGVLTTSTSKK1556 pKa = 10.66LAPAQYY1562 pKa = 6.91TTTWTTTNSDD1572 pKa = 3.26GSVEE1576 pKa = 4.08TDD1578 pKa = 2.95SGVVIVTTDD1587 pKa = 3.01SAGVLTTSTSKK1598 pKa = 10.87LSPAQYY1604 pKa = 6.53TTTWTTTNSDD1614 pKa = 3.26GSVEE1618 pKa = 4.08TDD1620 pKa = 2.96SGVVFVTTDD1629 pKa = 2.95SAGVLTTSTSKK1640 pKa = 10.66LAPAQYY1646 pKa = 6.91TTTWTTTNSDD1656 pKa = 3.26GSVEE1660 pKa = 4.08TDD1662 pKa = 2.95SGVVIVTTDD1671 pKa = 3.01SAGVLTTSTSKK1682 pKa = 10.66LAPAQYY1688 pKa = 6.91TTTWTTTNSDD1698 pKa = 3.26GSVEE1702 pKa = 4.08TDD1704 pKa = 2.96SGVVFVTTDD1713 pKa = 2.95SAGVLTTSTSKK1724 pKa = 10.66LAPAQYY1730 pKa = 6.91TTTWTTTNSDD1740 pKa = 3.26GSVEE1744 pKa = 4.08TDD1746 pKa = 2.96SGVVFVTTDD1755 pKa = 2.95SAGVLTTSTSKK1766 pKa = 10.57LAPASEE1772 pKa = 4.59VTSSGSSTISAEE1784 pKa = 4.05PTSLSTASPLINPAGLVGLDD1804 pKa = 3.86IITDD1808 pKa = 3.75ISVHH1812 pKa = 6.44DD1813 pKa = 4.16NSTGSFIGYY1822 pKa = 9.64KK1823 pKa = 10.06DD1824 pKa = 3.81DD1825 pKa = 4.23LFTVVVTLKK1834 pKa = 10.31SASSIPLGSITQFTVPEE1851 pKa = 4.15VFYY1854 pKa = 11.45GNLNPFEE1861 pKa = 4.59VYY1863 pKa = 10.55SGDD1866 pKa = 3.49GEE1868 pKa = 4.67YY1869 pKa = 10.93VGTISSDD1876 pKa = 3.26DD1877 pKa = 3.95SNVFTIVFDD1886 pKa = 4.19GKK1888 pKa = 10.84FGLTHH1893 pKa = 6.5TDD1895 pKa = 2.47IEE1897 pKa = 4.32AAFTFNVKK1905 pKa = 9.73LRR1907 pKa = 11.84YY1908 pKa = 6.26TTALTKK1914 pKa = 10.32RR1915 pKa = 11.84AEE1917 pKa = 4.07LSLDD1921 pKa = 3.54LSRR1924 pKa = 11.84PVAQPFVFTTVSSAFTSIIFFPDD1947 pKa = 2.98VSLDD1951 pKa = 4.0GIVGKK1956 pKa = 9.61VLIGDD1961 pKa = 3.81VYY1963 pKa = 11.43VFVGDD1968 pKa = 4.16ALSGASNSAISTGYY1982 pKa = 9.84SAPFSYY1988 pKa = 10.25WNNTVTATEE1997 pKa = 4.39TGTSLVTVTSCANGNCHH2014 pKa = 5.87GTSKK2018 pKa = 9.61LTSKK2022 pKa = 10.65ASSAVAPTTVSVTKK2036 pKa = 10.23DD2037 pKa = 2.93LTVIVTITSCSNHH2050 pKa = 4.77VCVPTTSKK2058 pKa = 11.19ALASIATTTVHH2069 pKa = 6.52GEE2071 pKa = 3.97VVLYY2075 pKa = 7.42TTYY2078 pKa = 10.89CPLSSEE2084 pKa = 4.37TGEE2087 pKa = 4.18KK2088 pKa = 10.05AAQEE2092 pKa = 4.4TSGSANSGSNSGSNAGSANAGSNAGSANAGSNSGSNAGSNSGSNAGSNSGSNSGSNAGSNSGSNAGSNSGSNAGSNSGSNSGSNAGSANAGSNVGSNAASGANVQYY2198 pKa = 9.43DD2199 pKa = 3.74TGISTLITATKK2210 pKa = 8.9STSSTSGNTGPAVSVNEE2227 pKa = 3.7NSARR2231 pKa = 11.84GSVNTSSFTAIFIGLLSLLTTFVV2254 pKa = 3.24

Molecular weight: 229.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L0BC87|A0A1L0BC87_9ASCO Protein YIP OS=[Candida] intermedia OX=45354 GN=SAMEA4029010_CIC11G00000003080 PE=3 SV=1
MM1 pKa = 7.42SSKK4 pKa = 9.35PTTPSLKK11 pKa = 10.07SRR13 pKa = 11.84EE14 pKa = 4.04AARR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84LLRR22 pKa = 11.84SSGVYY27 pKa = 10.11KK28 pKa = 10.65ILPQSPKK35 pKa = 8.54IHH37 pKa = 6.3YY38 pKa = 8.79PSEE41 pKa = 4.11VLPLEE46 pKa = 4.32SDD48 pKa = 4.37HH49 pKa = 7.38IQEE52 pKa = 4.5GLRR55 pKa = 11.84ALQEE59 pKa = 4.28SLGEE63 pKa = 4.28LEE65 pKa = 5.81SNMCEE70 pKa = 3.67LAKK73 pKa = 10.34IHH75 pKa = 6.19SAVNSGFNEE84 pKa = 3.73PFAAFLYY91 pKa = 10.7GLLITMFCNNFPGCPTRR108 pKa = 11.84DD109 pKa = 3.27TYY111 pKa = 11.33EE112 pKa = 3.9YY113 pKa = 11.06NQDD116 pKa = 3.21NGQKK120 pKa = 10.15EE121 pKa = 4.37RR122 pKa = 11.84VLLLQKK128 pKa = 10.07RR129 pKa = 11.84VHH131 pKa = 6.43AARR134 pKa = 11.84AEE136 pKa = 3.91NEE138 pKa = 3.96RR139 pKa = 11.84LKK141 pKa = 10.65QQLASQSTSQRR152 pKa = 11.84TIASRR157 pKa = 11.84PALSGQNQAIRR168 pKa = 11.84RR169 pKa = 11.84IGVPSGPSFGNSTRR183 pKa = 11.84GAPLSRR189 pKa = 11.84QKK191 pKa = 10.9KK192 pKa = 8.67VVVAHH197 pKa = 7.56DD198 pKa = 3.94DD199 pKa = 3.9TYY201 pKa = 11.05STTDD205 pKa = 3.16SFVDD209 pKa = 3.35IPTSSKK215 pKa = 10.03LASRR219 pKa = 11.84IQKK222 pKa = 9.08PATAVRR228 pKa = 11.84PGSLNSTGPNLDD240 pKa = 3.09QPPRR244 pKa = 11.84YY245 pKa = 9.17MRR247 pKa = 11.84GLFDD251 pKa = 3.77KK252 pKa = 10.32TSSSNVRR259 pKa = 11.84RR260 pKa = 11.84NLDD263 pKa = 3.18TKK265 pKa = 10.18TRR267 pKa = 11.84ARR269 pKa = 11.84GPTTSSARR277 pKa = 11.84RR278 pKa = 11.84QVEE281 pKa = 3.87RR282 pKa = 11.84ASRR285 pKa = 11.84LAGRR289 pKa = 11.84PPFRR293 pKa = 5.92

Molecular weight: 32.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5921

0

5921

2935372

22

5583

495.8

55.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.515 ± 0.04

1.121 ± 0.012

5.912 ± 0.028

6.654 ± 0.041

4.419 ± 0.026

5.583 ± 0.034

2.172 ± 0.018

5.723 ± 0.024

6.469 ± 0.04

10.091 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.055 ± 0.013

4.995 ± 0.021

4.547 ± 0.028

3.784 ± 0.026

4.414 ± 0.026

8.707 ± 0.07

5.894 ± 0.075

6.524 ± 0.026

1.064 ± 0.011

3.357 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski