Calibrachoa mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Alphacarmovirus

Average proteome isoelectric point is 8.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7E3L3|C7E3L3_9TOMB p8 OS=Calibrachoa mottle virus OX=204928 GN=P8 PE=4 SV=1
MM1 pKa = 8.01GILQLAKK8 pKa = 9.28EE9 pKa = 4.18LTVGGVCCTTLLCVGVAALEE29 pKa = 4.05VRR31 pKa = 11.84LALGAYY37 pKa = 7.36EE38 pKa = 4.74FNKK41 pKa = 10.03QCISNVRR48 pKa = 11.84GFIEE52 pKa = 4.74SRR54 pKa = 11.84GQSHH58 pKa = 7.13PSEE61 pKa = 4.2PKK63 pKa = 7.9YY64 pKa = 11.13AKK66 pKa = 10.74AEE68 pKa = 3.95VSPFQAEE75 pKa = 4.04LDD77 pKa = 4.13DD78 pKa = 5.55DD79 pKa = 5.17LEE81 pKa = 6.74DD82 pKa = 4.13DD83 pKa = 4.39AEE85 pKa = 4.13MRR87 pKa = 11.84NYY89 pKa = 10.54LEE91 pKa = 3.93KK92 pKa = 11.05HH93 pKa = 5.27EE94 pKa = 5.74DD95 pKa = 3.51KK96 pKa = 11.3EE97 pKa = 4.39KK98 pKa = 10.93DD99 pKa = 3.51DD100 pKa = 3.86EE101 pKa = 4.69GKK103 pKa = 10.67VVVKK107 pKa = 9.87NVRR110 pKa = 11.84TTLPRR115 pKa = 11.84NRR117 pKa = 11.84HH118 pKa = 4.44TKK120 pKa = 10.15GKK122 pKa = 9.1FLKK125 pKa = 10.64RR126 pKa = 11.84LVADD130 pKa = 4.46TKK132 pKa = 10.88NHH134 pKa = 6.8FGGTPTPTDD143 pKa = 3.56ANRR146 pKa = 11.84LAVMKK151 pKa = 10.44YY152 pKa = 7.02MVGRR156 pKa = 11.84CRR158 pKa = 11.84EE159 pKa = 3.71HH160 pKa = 8.46HH161 pKa = 6.43MVDD164 pKa = 2.8LHH166 pKa = 6.49IRR168 pKa = 11.84QVTEE172 pKa = 3.55LAKK175 pKa = 10.69AAVFTPDD182 pKa = 2.92ILEE185 pKa = 4.29VQSVQLLNSYY195 pKa = 8.84PAYY198 pKa = 10.19RR199 pKa = 11.84RR200 pKa = 11.84RR201 pKa = 11.84CALHH205 pKa = 6.82KK206 pKa = 10.26AHH208 pKa = 6.61QVQVWKK214 pKa = 10.5EE215 pKa = 3.53LLTNCFHH222 pKa = 7.1KK223 pKa = 10.91DD224 pKa = 2.4AWEE227 pKa = 4.25YY228 pKa = 10.37IWFRR232 pKa = 11.84MNGGLARR239 pKa = 11.84SPFQFHH245 pKa = 6.95KK246 pKa = 11.03

Molecular weight: 28.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7E3L4|C7E3L4_9TOMB p9 OS=Calibrachoa mottle virus OX=204928 GN=P9 PE=4 SV=1
MM1 pKa = 7.2EE2 pKa = 5.74RR3 pKa = 11.84EE4 pKa = 4.06HH5 pKa = 6.2PTINKK10 pKa = 9.83AISTKK15 pKa = 10.33EE16 pKa = 3.53KK17 pKa = 10.65SKK19 pKa = 10.75QLNSSKK25 pKa = 11.0DD26 pKa = 3.49KK27 pKa = 11.3NKK29 pKa = 10.66LSGKK33 pKa = 8.19LTAAKK38 pKa = 10.12AVANEE43 pKa = 3.87QARR46 pKa = 11.84GSVYY50 pKa = 10.52GGSFTNVARR59 pKa = 11.84EE60 pKa = 3.89IKK62 pKa = 9.86MEE64 pKa = 3.82IHH66 pKa = 6.22FHH68 pKa = 5.69FF69 pKa = 5.06

Molecular weight: 7.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1502

69

762

300.4

33.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.19 ± 0.533

2.397 ± 0.66

4.461 ± 0.322

5.726 ± 0.825

3.928 ± 0.319

6.658 ± 0.352

2.73 ± 0.715

4.993 ± 0.711

7.457 ± 0.592

8.455 ± 0.364

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.271

4.128 ± 0.544

4.727 ± 0.476

3.995 ± 0.597

5.925 ± 0.74

6.125 ± 1.267

6.258 ± 1.544

7.124 ± 0.443

1.398 ± 0.23

3.728 ± 0.326
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski