Armillaria solidipes
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20715 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H3CLT8|A0A2H3CLT8_9AGAR FAS1 domain-containing protein OS=Armillaria solidipes OX=1076256 GN=ARMSODRAFT_1010383 PE=4 SV=1
MM1 pKa = 6.9 KK2 pKa = 9.87 TLVLSLLSIPFSAVAQYY19 pKa = 7.79 TWKK22 pKa = 10.31 NVKK25 pKa = 9.87 IGGGGGFVPGIVFNPSEE42 pKa = 4.14 EE43 pKa = 3.74 GLAYY47 pKa = 10.6 VRR49 pKa = 11.84 TDD51 pKa = 2.33 IGGAYY56 pKa = 9.96 RR57 pKa = 11.84 LNDD60 pKa = 4.05 DD61 pKa = 4.65 DD62 pKa = 4.02 TWTPLTDD69 pKa = 3.14 WVDD72 pKa = 3.27 NDD74 pKa = 3.56 RR75 pKa = 11.84 WNMWGVDD82 pKa = 3.84 ALATDD87 pKa = 3.79 PVEE90 pKa = 4.25 TNRR93 pKa = 11.84 LYY95 pKa = 11.04 LATGMYY101 pKa = 8.35 TNSWDD106 pKa = 3.67 PDD108 pKa = 3.38 NGHH111 pKa = 6.67 ILVSEE116 pKa = 4.68 DD117 pKa = 3.36 YY118 pKa = 11.0 GEE120 pKa = 4.96 SFAQVDD126 pKa = 4.29 LPFKK130 pKa = 10.91 VGGNMPGRR138 pKa = 11.84 GVGEE142 pKa = 4.12 RR143 pKa = 11.84 LAVDD147 pKa = 3.76 PNSNNILFFGARR159 pKa = 11.84 SGNGLYY165 pKa = 10.58 KK166 pKa = 10.06 STDD169 pKa = 3.5 YY170 pKa = 11.45 GSTWTQVSSLPDD182 pKa = 3.1 VGTYY186 pKa = 10.28 VVDD189 pKa = 3.6 ATDD192 pKa = 3.34 TSGYY196 pKa = 10.72 NSDD199 pKa = 3.8 PLGIAFVTFDD209 pKa = 3.91 STSGSSGSPTPRR221 pKa = 11.84 VFVGVASNGTDD232 pKa = 3.1 NIFVSEE238 pKa = 4.33 DD239 pKa = 3.65 AGDD242 pKa = 3.32 TWTAIAGQQQTYY254 pKa = 9.21 FPHH257 pKa = 6.91 KK258 pKa = 10.37 GVLSPDD264 pKa = 3.08 EE265 pKa = 4.2 GLLYY269 pKa = 10.7 VSYY272 pKa = 11.46 SDD274 pKa = 3.5 GTGPYY279 pKa = 10.01 DD280 pKa = 3.46 GTLGAVYY287 pKa = 10.0 KK288 pKa = 10.7 YY289 pKa = 10.77 DD290 pKa = 3.38 IANATWTDD298 pKa = 3.2 ITPVSGSDD306 pKa = 3.15 LSFGFGGLSVDD317 pKa = 3.79 VKK319 pKa = 11.41 NPGTLMVAALNLWWPDD335 pKa = 3.16 GQIYY339 pKa = 10.44 RR340 pKa = 11.84 STDD343 pKa = 2.97 SGATWSTFWTWGAYY357 pKa = 9.26 PEE359 pKa = 4.07 INKK362 pKa = 10.02 YY363 pKa = 10.5 YY364 pKa = 10.5 SYY366 pKa = 11.31 SDD368 pKa = 4.29 SLAPWLGVDD377 pKa = 4.74 YY378 pKa = 11.46 VDD380 pKa = 3.63 TTLGDD385 pKa = 3.8 KK386 pKa = 10.73 QIGWMMEE393 pKa = 3.94 ALSIDD398 pKa = 4.37 PFDD401 pKa = 4.74 SDD403 pKa = 3.46 HH404 pKa = 6.59 FLYY407 pKa = 9.0 GTGATIYY414 pKa = 10.49 GSRR417 pKa = 11.84 DD418 pKa = 2.97 LTLWDD423 pKa = 3.65 SVHH426 pKa = 6.48 NISLSSMADD435 pKa = 3.66 GIEE438 pKa = 4.06 EE439 pKa = 4.12 TAVQALISPPGGPSLISGVSDD460 pKa = 2.82 IQGFVHH466 pKa = 6.83 TDD468 pKa = 3.07 LGTPTTSFSSPVWTSVVDD486 pKa = 4.17 LDD488 pKa = 4.17 YY489 pKa = 11.57 AGNVPTNIIRR499 pKa = 11.84 LGNGDD504 pKa = 3.83 SSTGKK509 pKa = 9.78 QVAISTDD516 pKa = 3.44 SGTTWSQDD524 pKa = 2.25 TGAPDD529 pKa = 3.4 NVSGGKK535 pKa = 9.21 IAISADD541 pKa = 2.9 GDD543 pKa = 4.0 TVLWRR548 pKa = 11.84 TSSNGVLVSQNTSAFTTVSTLPTSAVIASDD578 pKa = 3.23 KK579 pKa = 10.01 TNNSVFYY586 pKa = 9.69 GASSSSFYY594 pKa = 11.03 VSNDD598 pKa = 3.07 TGSTFSAVSTLGNSTSPVKK617 pKa = 10.43 IVVNPDD623 pKa = 2.7 VAGDD627 pKa = 3.5 VWVSTDD633 pKa = 2.7 VGLFHH638 pKa = 6.53 STDD641 pKa = 3.43 YY642 pKa = 11.38 GSTFMAIEE650 pKa = 4.4 SVSQAWAIALGTAATDD666 pKa = 3.46 GGYY669 pKa = 9.48 PAVFAAANIGDD680 pKa = 3.65 GVGYY684 pKa = 10.27 YY685 pKa = 10.44 RR686 pKa = 11.84 SDD688 pKa = 3.87 DD689 pKa = 3.96 EE690 pKa = 4.68 GATWTQINDD699 pKa = 3.19 SSLGFSSVSANVMAADD715 pKa = 3.41 PRR717 pKa = 11.84 VYY719 pKa = 10.28 GRR721 pKa = 11.84 VYY723 pKa = 10.17 IGTNGRR729 pKa = 11.84 GIFYY733 pKa = 10.77 GDD735 pKa = 3.25 ISSSGTSTSSTVGPSSSAAVSTSAAAASSAVTSSIQASPSATTASSSEE783 pKa = 4.19 NINGSGSQRR792 pKa = 11.84 LL793 pKa = 3.52
Molecular weight: 83.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.834
IPC_protein 3.897
Toseland 3.656
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.872
Rodwell 3.719
Grimsley 3.554
Solomon 3.91
Lehninger 3.859
Nozaki 4.012
DTASelect 4.329
Thurlkill 3.706
EMBOSS 3.884
Sillero 4.024
Patrickios 1.252
IPC_peptide 3.897
IPC2_peptide 3.999
IPC2.peptide.svr19 3.9
Protein with the highest isoelectric point:
>tr|A0A2H3C3P5|A0A2H3C3P5_9AGAR Uncharacterized protein OS=Armillaria solidipes OX=1076256 GN=ARMSODRAFT_1017547 PE=4 SV=1
SS1 pKa = 5.73 TQVQRR6 pKa = 11.84 PASAIQRR13 pKa = 11.84 PTSIIQRR20 pKa = 11.84 PASVVQRR27 pKa = 11.84 PSSVIQRR34 pKa = 11.84 PASITQRR41 pKa = 11.84 PASAAQRR48 pKa = 11.84 STLAIQHH55 pKa = 6.54 PASAAQRR62 pKa = 11.84 QPLAVQQQ69 pKa = 3.7
Molecular weight: 7.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.485
IPC2_protein 11.067
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.34
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.106
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.163
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
20715
0
20715
7685379
49
4986
371.0
41.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.774 ± 0.017
1.494 ± 0.007
5.687 ± 0.012
5.675 ± 0.016
3.952 ± 0.011
6.192 ± 0.017
2.593 ± 0.008
5.295 ± 0.011
4.444 ± 0.018
9.528 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.206 ± 0.007
3.473 ± 0.009
6.162 ± 0.018
3.554 ± 0.01
6.103 ± 0.015
8.803 ± 0.022
6.086 ± 0.011
6.501 ± 0.011
1.579 ± 0.007
2.899 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here