Sulfobacillus sp. hq2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Clostridiales Family XVII. Incertae Sedis; Sulfobacillus; unclassified Sulfobacillus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3298 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S3QGP8|A0A2S3QGP8_9FIRM RNA polymerase sigma factor OS=Sulfobacillus sp. hq2 OX=2039167 GN=CO251_08675 PE=3 SV=1
MM1 pKa = 7.73ADD3 pKa = 2.96LRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84VSHH10 pKa = 6.21LVGMAQRR17 pKa = 11.84YY18 pKa = 9.01DD19 pKa = 3.25VGSRR23 pKa = 11.84SRR25 pKa = 11.84EE26 pKa = 3.59GKK28 pKa = 10.13IIEE31 pKa = 4.62EE32 pKa = 4.47IIDD35 pKa = 3.62VLRR38 pKa = 11.84DD39 pKa = 3.26LTLDD43 pKa = 3.71VEE45 pKa = 4.73EE46 pKa = 4.52VTANQVEE53 pKa = 4.38IEE55 pKa = 4.44DD56 pKa = 4.36FLDD59 pKa = 4.38EE60 pKa = 5.12LDD62 pKa = 4.3EE63 pKa = 5.04DD64 pKa = 4.35LLDD67 pKa = 4.5VEE69 pKa = 5.09QEE71 pKa = 4.42LYY73 pKa = 11.1DD74 pKa = 5.77DD75 pKa = 5.0GDD77 pKa = 4.25DD78 pKa = 5.86DD79 pKa = 6.34IEE81 pKa = 5.9FDD83 pKa = 6.1DD84 pKa = 6.56DD85 pKa = 4.52DD86 pKa = 4.2TLDD89 pKa = 4.65DD90 pKa = 4.64EE91 pKa = 5.32GLVGNGFDD99 pKa = 4.73EE100 pKa = 4.79EE101 pKa = 4.2PSYY104 pKa = 10.96IALEE108 pKa = 4.22CPVCHH113 pKa = 6.99RR114 pKa = 11.84EE115 pKa = 3.61SSYY118 pKa = 11.46NGEE121 pKa = 4.1LFDD124 pKa = 6.13RR125 pKa = 11.84DD126 pKa = 5.14DD127 pKa = 3.8IQLSCPHH134 pKa = 7.12CGNVIYY140 pKa = 10.47DD141 pKa = 3.85SEE143 pKa = 4.45EE144 pKa = 3.74DD145 pKa = 4.32CIIMDD150 pKa = 5.74DD151 pKa = 4.09ADD153 pKa = 4.02EE154 pKa = 5.48DD155 pKa = 4.31DD156 pKa = 4.67GPGYY160 pKa = 8.15TAYY163 pKa = 10.49HH164 pKa = 6.5

Molecular weight: 18.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S3QKI2|A0A2S3QKI2_9FIRM Uncharacterized protein OS=Sulfobacillus sp. hq2 OX=2039167 GN=CO251_00380 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNRR9 pKa = 11.84RR10 pKa = 11.84HH11 pKa = 5.61RR12 pKa = 11.84AKK14 pKa = 10.32VHH16 pKa = 5.33GFRR19 pKa = 11.84KK20 pKa = 10.02RR21 pKa = 11.84MSTKK25 pKa = 9.93AGRR28 pKa = 11.84MVIKK32 pKa = 10.46RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.26GRR39 pKa = 11.84KK40 pKa = 8.7RR41 pKa = 11.84LAGG44 pKa = 3.61

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3298

0

3298

984618

27

1424

298.6

32.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.141 ± 0.048

0.634 ± 0.015

4.766 ± 0.037

5.094 ± 0.045

3.531 ± 0.027

7.79 ± 0.038

2.905 ± 0.024

5.586 ± 0.026

2.985 ± 0.027

10.59 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.709 ± 0.02

2.704 ± 0.028

5.403 ± 0.032

4.524 ± 0.032

6.114 ± 0.042

5.717 ± 0.037

5.773 ± 0.035

8.137 ± 0.035

2.083 ± 0.026

2.815 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski