Verticillium dahliae partitivirus 1
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M3LA90|A0A0M3LA90_9VIRU RNA-dependent RNA polymerase OS=Verticillium dahliae partitivirus 1 OX=1310424 GN=RdRp PE=4 SV=1
MM1 pKa = 8.04 ADD3 pKa = 3.53 TQSQLPSTVAPSDD16 pKa = 3.72 SASTAGRR23 pKa = 11.84 KK24 pKa = 8.95 SKK26 pKa = 10.2 PGKK29 pKa = 10.18 AEE31 pKa = 3.65 RR32 pKa = 11.84 QARR35 pKa = 11.84 RR36 pKa = 11.84 SAVGSQPGQAASASKK51 pKa = 10.84 ASAFSSGISAPKK63 pKa = 8.53 PQPGKK68 pKa = 10.6 YY69 pKa = 9.01 PVVFQTGAGEE79 pKa = 4.02 PARR82 pKa = 11.84 DD83 pKa = 3.25 QTFALDD89 pKa = 3.5 EE90 pKa = 4.38 RR91 pKa = 11.84 VLEE94 pKa = 4.24 RR95 pKa = 11.84 SLRR98 pKa = 11.84 TFPDD102 pKa = 2.92 RR103 pKa = 11.84 FTFNAKK109 pKa = 8.79 YY110 pKa = 10.89 AEE112 pKa = 4.41 FKK114 pKa = 10.66 SHH116 pKa = 7.52 AEE118 pKa = 3.79 IDD120 pKa = 3.56 DD121 pKa = 4.0 HH122 pKa = 7.05 EE123 pKa = 4.45 FTKK126 pKa = 10.99 DD127 pKa = 3.37 LVVAALLRR135 pKa = 11.84 LAQQVVHH142 pKa = 5.67 SHH144 pKa = 5.41 VNMGLPQGDD153 pKa = 4.28 FAPVASSDD161 pKa = 3.46 VRR163 pKa = 11.84 VPASLAAFISQFGEE177 pKa = 4.1 FSVPTLGTRR186 pKa = 11.84 FLFSDD191 pKa = 3.82 YY192 pKa = 10.61 EE193 pKa = 4.14 NTVRR197 pKa = 11.84 SIVWMAEE204 pKa = 3.74 GVKK207 pKa = 10.56 RR208 pKa = 11.84 NGIGGAPLKK217 pKa = 10.64 RR218 pKa = 11.84 SWLPVRR224 pKa = 11.84 PSDD227 pKa = 3.33 GHH229 pKa = 5.6 TKK231 pKa = 9.31 TVIASRR237 pKa = 11.84 LCEE240 pKa = 3.97 LLAQAEE246 pKa = 4.37 LAVDD250 pKa = 4.04 PDD252 pKa = 3.8 ALEE255 pKa = 4.59 GGVLSGEE262 pKa = 4.45 IPDD265 pKa = 3.29 AWEE268 pKa = 4.08 SIKK271 pKa = 10.71 PVLGDD276 pKa = 3.09 NDD278 pKa = 3.4 EE279 pKa = 4.21 RR280 pKa = 11.84 RR281 pKa = 11.84 DD282 pKa = 3.69 RR283 pKa = 11.84 FDD285 pKa = 5.13 FLFKK289 pKa = 10.45 SYY291 pKa = 10.57 RR292 pKa = 11.84 DD293 pKa = 3.35 APAFVTAFTTASASGVLAEE312 pKa = 6.06 LDD314 pKa = 4.06 LSWDD318 pKa = 3.78 RR319 pKa = 11.84 PSAGHH324 pKa = 6.45 VDD326 pKa = 2.89 WTFNPKK332 pKa = 9.7 EE333 pKa = 4.09 VFTRR337 pKa = 11.84 LSDD340 pKa = 2.85 AWARR344 pKa = 11.84 RR345 pKa = 11.84 STTYY349 pKa = 11.61 ALFFEE354 pKa = 5.28 LSSSQMTRR362 pKa = 11.84 NVSAGSQSQMARR374 pKa = 11.84 VNTRR378 pKa = 11.84 DD379 pKa = 2.96 GVTVVKK385 pKa = 9.06 THH387 pKa = 6.95 LGLSAPEE394 pKa = 4.12 FSLVACFPASVIFTGGLTRR413 pKa = 11.84 RR414 pKa = 11.84 VVVTTPLSVEE424 pKa = 3.73 QRR426 pKa = 11.84 ATEE429 pKa = 4.28 FVQMDD434 pKa = 3.21 WRR436 pKa = 5.26
Molecular weight: 47.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.639
IPC2_protein 6.576
IPC_protein 6.883
Toseland 7.029
ProMoST 7.424
Dawson 7.337
Bjellqvist 7.073
Wikipedia 7.322
Rodwell 7.307
Grimsley 7.146
Solomon 7.439
Lehninger 7.439
Nozaki 7.424
DTASelect 7.585
Thurlkill 7.614
EMBOSS 7.702
Sillero 7.673
Patrickios 4.978
IPC_peptide 7.439
IPC2_peptide 7.073
IPC2.peptide.svr19 7.061
Protein with the highest isoelectric point:
>tr|A0A0M3LA90|A0A0M3LA90_9VIRU RNA-dependent RNA polymerase OS=Verticillium dahliae partitivirus 1 OX=1310424 GN=RdRp PE=4 SV=1
MM1 pKa = 7.93 EE2 pKa = 6.77 DD3 pKa = 3.42 FTQDD7 pKa = 3.31 PTTHH11 pKa = 6.67 NIVAEE16 pKa = 4.58 GSHH19 pKa = 7.06 LIDD22 pKa = 4.56 ALHH25 pKa = 6.35 LRR27 pKa = 11.84 PPKK30 pKa = 10.28 SRR32 pKa = 11.84 SATSEE37 pKa = 4.03 DD38 pKa = 3.8 VVSSNFRR45 pKa = 11.84 SPNLAEE51 pKa = 3.65 IAKK54 pKa = 9.21 YY55 pKa = 10.3 GGYY58 pKa = 7.94 STYY61 pKa = 11.11 SSNSNTDD68 pKa = 2.97 PYY70 pKa = 11.29 VRR72 pKa = 11.84 EE73 pKa = 3.98 TLKK76 pKa = 10.98 LFSRR80 pKa = 11.84 DD81 pKa = 3.06 TYY83 pKa = 10.88 EE84 pKa = 5.05 DD85 pKa = 2.74 IRR87 pKa = 11.84 GFTRR91 pKa = 11.84 RR92 pKa = 11.84 PQGTPGMYY100 pKa = 8.42 TALKK104 pKa = 9.81 KK105 pKa = 10.83 FSGEE109 pKa = 3.94 RR110 pKa = 11.84 NTFGDD115 pKa = 4.73 LSPSQQSSMRR125 pKa = 11.84 RR126 pKa = 11.84 AIGKK130 pKa = 9.39 AKK132 pKa = 10.31 KK133 pKa = 10.09 AFKK136 pKa = 10.39 LPYY139 pKa = 9.55 KK140 pKa = 10.34 RR141 pKa = 11.84 EE142 pKa = 3.91 PLDD145 pKa = 3.15 WHH147 pKa = 6.48 EE148 pKa = 4.21 VGQFLRR154 pKa = 11.84 RR155 pKa = 11.84 DD156 pKa = 3.56 TAAGATFMGQKK167 pKa = 10.11 KK168 pKa = 9.68 GDD170 pKa = 3.59 VMEE173 pKa = 5.62 AIYY176 pKa = 10.8 HH177 pKa = 5.12 EE178 pKa = 4.58 ARR180 pKa = 11.84 WLGHH184 pKa = 6.1 RR185 pKa = 11.84 MKK187 pKa = 10.64 QDD189 pKa = 2.56 GRR191 pKa = 11.84 AGFDD195 pKa = 3.4 PTRR198 pKa = 11.84 MRR200 pKa = 11.84 FPPCLAGQRR209 pKa = 11.84 GGMSEE214 pKa = 3.87 IDD216 pKa = 3.64 DD217 pKa = 4.28 PKK219 pKa = 11.01 TRR221 pKa = 11.84 LVWIYY226 pKa = 8.84 PAEE229 pKa = 4.02 MLVVEE234 pKa = 5.03 GFYY237 pKa = 11.12 APLMYY242 pKa = 9.66 RR243 pKa = 11.84 DD244 pKa = 4.75 FMSDD248 pKa = 3.17 PNSPMLNGKK257 pKa = 8.31 SAQRR261 pKa = 11.84 LYY263 pKa = 10.74 TEE265 pKa = 4.78 WCCKK269 pKa = 10.29 LRR271 pKa = 11.84 DD272 pKa = 3.54 GEE274 pKa = 4.38 TLYY277 pKa = 11.52 GIDD280 pKa = 4.38 FSSFDD285 pKa = 3.59 TKK287 pKa = 10.85 VPAWLIRR294 pKa = 11.84 VAFDD298 pKa = 4.45 ILRR301 pKa = 11.84 QNINFEE307 pKa = 4.4 TFGGKK312 pKa = 9.0 PVDD315 pKa = 3.87 KK316 pKa = 10.81 QDD318 pKa = 3.18 AQKK321 pKa = 9.9 WRR323 pKa = 11.84 NVWDD327 pKa = 3.45 AMVWYY332 pKa = 8.66 FINTPILMPDD342 pKa = 2.44 GRR344 pKa = 11.84 MFRR347 pKa = 11.84 KK348 pKa = 9.65 FRR350 pKa = 11.84 GVPSGSWWTQMIDD363 pKa = 3.35 SVVNHH368 pKa = 6.47 ILIDD372 pKa = 3.82 YY373 pKa = 9.97 LADD376 pKa = 3.6 CQRR379 pKa = 11.84 VEE381 pKa = 3.79 IRR383 pKa = 11.84 NLRR386 pKa = 11.84 VLGDD390 pKa = 3.83 DD391 pKa = 4.09 SAFCSGDD398 pKa = 3.46 QFDD401 pKa = 5.38 LEE403 pKa = 4.38 LAKK406 pKa = 10.61 GDD408 pKa = 4.12 CEE410 pKa = 4.14 PTGMVIKK417 pKa = 10.24 PEE419 pKa = 3.85 KK420 pKa = 10.31 CEE422 pKa = 3.87 RR423 pKa = 11.84 TKK425 pKa = 11.38 DD426 pKa = 3.35 PGEE429 pKa = 4.12 FKK431 pKa = 11.16 LLGTTYY437 pKa = 10.59 RR438 pKa = 11.84 GGHH441 pKa = 5.07 VFRR444 pKa = 11.84 DD445 pKa = 3.58 TEE447 pKa = 4.14 EE448 pKa = 3.94 WFKK451 pKa = 11.12 LALYY455 pKa = 9.52 PEE457 pKa = 4.73 SSVLTLDD464 pKa = 3.19 ISFTRR469 pKa = 11.84 LIGLWLGGAMWDD481 pKa = 4.3 KK482 pKa = 10.88 RR483 pKa = 11.84 FCEE486 pKa = 4.09 YY487 pKa = 10.34 MDD489 pKa = 4.67 FFQSSYY495 pKa = 10.42 PCPEE499 pKa = 4.29 EE500 pKa = 4.24 GWFSKK505 pKa = 10.13 DD506 pKa = 3.02 QKK508 pKa = 10.51 RR509 pKa = 11.84 WLEE512 pKa = 3.89 IIYY515 pKa = 10.23 SGKK518 pKa = 10.27 APRR521 pKa = 11.84 GWTSKK526 pKa = 10.79 KK527 pKa = 9.83 SLFWRR532 pKa = 11.84 SIFYY536 pKa = 10.51 AYY538 pKa = 10.44 GG539 pKa = 3.3
Molecular weight: 62.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.197
IPC2_protein 7.644
IPC_protein 7.497
Toseland 7.161
ProMoST 8.053
Dawson 8.214
Bjellqvist 8.521
Wikipedia 8.156
Rodwell 8.244
Grimsley 7.19
Solomon 8.287
Lehninger 8.302
Nozaki 8.668
DTASelect 8.287
Thurlkill 8.346
EMBOSS 8.419
Sillero 8.653
Patrickios 4.431
IPC_peptide 8.287
IPC2_peptide 7.424
IPC2.peptide.svr19 7.695
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
975
436
539
487.5
54.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.308 ± 2.015
1.128 ± 0.398
6.667 ± 0.418
5.333 ± 0.171
6.154 ± 0.023
7.282 ± 0.511
1.538 ± 0.04
3.385 ± 0.785
5.026 ± 0.806
7.487 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.564 ± 0.706
2.256 ± 0.251
5.744 ± 0.131
3.59 ± 0.32
7.487 ± 0.088
8.615 ± 1.15
6.051 ± 0.357
6.154 ± 1.523
2.462 ± 0.509
2.769 ± 0.965
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here