Candidatus Erwinia dacicola

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Erwinia

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3896 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E7Z1B0|A0A1E7Z1B0_9GAMM ATP synthase subunit c OS=Candidatus Erwinia dacicola OX=252393 GN=atpE PE=3 SV=1
MM1 pKa = 7.67NIGLFYY7 pKa = 10.91GSSTCYY13 pKa = 10.49TEE15 pKa = 3.84MAAEE19 pKa = 5.11KK20 pKa = 10.16IRR22 pKa = 11.84DD23 pKa = 4.29FIGEE27 pKa = 4.21DD28 pKa = 3.73LVTLHH33 pKa = 6.35NLKK36 pKa = 10.19EE37 pKa = 4.13DD38 pKa = 3.7SPALMEE44 pKa = 4.87QYY46 pKa = 11.17DD47 pKa = 4.48LLILGIPTWDD57 pKa = 3.34FGEE60 pKa = 4.35LQEE63 pKa = 4.51DD64 pKa = 4.27WEE66 pKa = 5.11AIWTDD71 pKa = 4.0LPTLNLQDD79 pKa = 4.24KK80 pKa = 10.11VVALYY85 pKa = 10.78GIGDD89 pKa = 3.5QGEE92 pKa = 4.14YY93 pKa = 10.77SEE95 pKa = 4.9WFLDD99 pKa = 3.7ALGMLHH105 pKa = 6.87EE106 pKa = 4.84VLAQSGVKK114 pKa = 9.28FVGYY118 pKa = 9.24WPLAGYY124 pKa = 9.23EE125 pKa = 4.16FTSTKK130 pKa = 10.36PLTADD135 pKa = 3.27GQKK138 pKa = 10.36FVGLALDD145 pKa = 5.71DD146 pKa = 3.5ITQFEE151 pKa = 4.4QTDD154 pKa = 3.35EE155 pKa = 6.14RR156 pKa = 11.84IAQWCEE162 pKa = 3.62QILTEE167 pKa = 4.07MAEE170 pKa = 4.18MLL172 pKa = 4.15

Molecular weight: 19.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E7YW38|A0A1E7YW38_9GAMM Esterase OS=Candidatus Erwinia dacicola OX=252393 GN=ACZ87_02270 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MANKK25 pKa = 10.03NGRR28 pKa = 11.84QLLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.2GRR39 pKa = 11.84SRR41 pKa = 11.84LTVSKK46 pKa = 11.03

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3896

0

3896

726939

13

1843

186.6

20.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.514 ± 0.057

1.138 ± 0.016

5.363 ± 0.039

5.897 ± 0.054

3.675 ± 0.037

7.103 ± 0.047

2.248 ± 0.024

5.686 ± 0.039

4.85 ± 0.036

10.369 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.885 ± 0.022

3.856 ± 0.029

4.219 ± 0.028

4.588 ± 0.037

6.027 ± 0.044

6.195 ± 0.044

5.327 ± 0.032

6.919 ± 0.035

1.374 ± 0.021

2.764 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski