Dyella psychrodurans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Rhodanobacteraceae; Dyella

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4047 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A370X1A9|A0A370X1A9_9GAMM GGDEF domain-containing protein OS=Dyella psychrodurans OX=1927960 GN=DWU99_16485 PE=4 SV=1
MM1 pKa = 7.85RR2 pKa = 11.84ISTSWMYY9 pKa = 9.96QQSLSTMLNQEE20 pKa = 4.13SEE22 pKa = 4.34LAQTQNEE29 pKa = 4.55VSTGNAINVPSDD41 pKa = 3.55NPTGAAQIVGLNHH54 pKa = 6.54ILAEE58 pKa = 4.09NSEE61 pKa = 4.31YY62 pKa = 10.9TNNISSANTRR72 pKa = 11.84LSTEE76 pKa = 3.97TSTLTSVSNLLSSVNDD92 pKa = 3.34IALGGINSAMSPSDD106 pKa = 4.02LSNMATQLTQYY117 pKa = 10.66RR118 pKa = 11.84NQLVQLANTTDD129 pKa = 3.58ANGQALFSGTSSTTTPFVTNSSTGAVTYY157 pKa = 10.55AGNDD161 pKa = 3.18QQTFVAVGTGLQVASGDD178 pKa = 3.8PGSSIFMNLAQGNGSFVSSAGSSNTGTLVVGANSVTSTTAFAAATAAGPINDD230 pKa = 4.91TITFGANGTYY240 pKa = 10.67AVTDD244 pKa = 3.58AAGNPVTDD252 pKa = 3.61SSGNPITGTYY262 pKa = 9.06TDD264 pKa = 4.23GGSITFDD271 pKa = 2.98GMSITMSGTPAAGDD285 pKa = 3.76TVKK288 pKa = 10.65VQSDD292 pKa = 3.89TASNTQDD299 pKa = 3.69VFTTLNNMIAALQSGGSTTAISNTLNRR326 pKa = 11.84QLEE329 pKa = 4.45SLNQAMNSVSSAQVSVGSRR348 pKa = 11.84VDD350 pKa = 3.29TLQQQGSSYY359 pKa = 11.4SDD361 pKa = 3.72LNVTYY366 pKa = 10.43KK367 pKa = 10.68SALSDD372 pKa = 3.61VQNVDD377 pKa = 2.84MATAISNLSLQSTALQASQQVFAKK401 pKa = 10.48VQGTSLFNYY410 pKa = 9.72LQGG413 pKa = 3.7

Molecular weight: 42.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A370WZF6|A0A370WZF6_9GAMM Uncharacterized protein OS=Dyella psychrodurans OX=1927960 GN=DWU99_17630 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.06LKK11 pKa = 10.38RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATADD26 pKa = 3.43GRR28 pKa = 11.84KK29 pKa = 9.27VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.95GRR39 pKa = 11.84KK40 pKa = 8.92RR41 pKa = 11.84LIPP44 pKa = 4.02

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4047

0

4047

1326686

29

2898

327.8

35.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.758 ± 0.055

0.889 ± 0.012

5.697 ± 0.032

4.875 ± 0.043

3.512 ± 0.026

8.116 ± 0.041

2.605 ± 0.02

4.678 ± 0.027

3.106 ± 0.033

10.458 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.397 ± 0.019

3.082 ± 0.034

5.14 ± 0.026

3.988 ± 0.027

6.636 ± 0.047

5.911 ± 0.042

5.47 ± 0.05

7.437 ± 0.033

1.563 ± 0.022

2.683 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski